GREMLIN Database
THIN - Thiamine pyrophosphokinase
UniProt: O34664 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 214 (204)
Sequences: 2139 (1788)
Seq/√Len: 125.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
169_L172_F4.3371.00
73_Y84_L3.9081.00
173_K192_E3.3281.00
8_A82_L3.2371.00
76_E173_K3.1511.00
170_T199_T3.0351.00
155_I172_F2.9901.00
88_W92_K2.7551.00
9_G33_K2.5771.00
29_I89_A2.4651.00
193_L198_G2.3951.00
165_H204_K2.2971.00
79_Q87_D2.0481.00
176_L179_C2.0071.00
128_R135_H1.9941.00
166_E203_A1.9891.00
47_E88_W1.9891.00
178_N203_A1.9591.00
69_A92_K1.9321.00
33_K36_V1.8941.00
31_V85_A1.8811.00
166_E178_N1.8801.00
143_E201_S1.8731.00
170_T177_N1.8531.00
99_I102_I1.8471.00
88_W91_E1.8451.00
96_I126_K1.8241.00
27_L46_V1.8181.00
172_F198_G1.8061.00
7_V38_L1.7781.00
120_G127_I1.7671.00
145_D197_R1.7581.00
107_A110_F1.7451.00
154_Y192_E1.7241.00
48_A63_I1.6841.00
143_E199_T1.6431.00
155_I198_G1.6131.00
4_I94_P1.5941.00
83_D119_K1.5761.00
36_V59_E1.5671.00
105_G133_Q1.5481.00
117_L127_I1.4981.00
98_Q128_R1.4961.00
82_L99_I1.4871.00
103_T110_F1.4301.00
106_R190_S1.4131.00
139_F144_Y1.4081.00
80_T189_I1.4031.00
36_V56_T1.4011.00
131_D134_N1.3881.00
77_K84_L1.3881.00
117_L138_M1.3861.00
60_R64_E1.3751.00
157_F169_L1.3731.00
37_T41_A1.3511.00
147_E197_R1.3411.00
30_G38_L1.3401.00
39_L59_E1.3191.00
35_T55_I1.3081.00
90_L125_I1.3051.00
56_T59_E1.2781.00
145_D199_T1.2651.00
179_C187_L1.2571.00
11_P105_G1.2561.00
38_L43_I1.2521.00
166_E207_L1.2511.00
96_I128_R1.2451.00
59_E63_I1.2401.00
8_A102_I1.2350.99
168_T177_N1.2250.99
165_H180_H1.2250.99
39_L63_I1.2240.99
61_R65_K1.2180.99
5_N25_H1.2150.99
79_Q84_L1.2030.99
45_P48_A1.1980.99
71_H88_W1.1920.99
169_L179_C1.1790.99
28_W43_I1.1750.99
39_L62_R1.1660.99
139_F200_F1.1610.99
156_S189_I1.1590.99
55_I63_I1.1570.99
172_F193_L1.1490.99
99_I129_L1.1470.99
64_E72_V1.1450.99
172_F175_P1.1410.99
6_I31_V1.1290.99
103_T136_I1.1160.99
89_A97_I1.0730.99
61_R64_E1.0680.99
86_L97_I1.0660.98
160_F183_L1.0640.98
6_I89_A1.0640.98
27_L47_E1.0640.98
25_H98_Q1.0570.98
118_Y183_L1.0560.98
170_T175_P1.0520.98
113_N129_L1.0480.98
36_V39_L1.0390.98
157_F188_C1.0380.98
4_I93_Q1.0140.98
74_Q77_K1.0130.98
98_Q130_I1.0040.98
49_F85_A0.9940.98
27_L92_K0.9850.97
202_F205_G0.9830.97
181_I188_C0.9810.97
30_G35_T0.9790.97
107_A136_I0.9750.97
36_V55_I0.9720.97
138_M160_F0.9670.97
5_N100_F0.9640.97
137_Q209_M0.9600.97
21_Y100_F0.9510.97
182_T185_S0.9470.97
173_K194_I0.9440.97
134_N154_Y0.9370.97
157_F209_M0.9280.96
139_F209_M0.9200.96
57_E61_R0.9180.96
166_E180_H0.9160.96
30_G48_A0.9070.96
168_T178_N0.9050.96
7_V30_G0.9020.96
102_I113_N0.8960.96
31_V34_G0.8950.96
29_I49_F0.8940.96
49_F88_W0.8870.95
48_A52_F0.8840.95
158_I189_I0.8840.95
13_N132_K0.8800.95
29_I85_A0.8750.95
47_E92_K0.8750.95
121_V140_P0.8710.95
202_F206_I0.8620.95
157_F207_L0.8580.94
103_T131_D0.8550.94
76_E79_Q0.8520.94
22_T28_W0.8460.94
110_F114_I0.8450.94
109_H113_N0.8390.94
63_I68_P0.8290.93
148_K198_G0.8280.93
55_I60_R0.8240.93
6_I29_I0.8210.93
57_E60_R0.8200.93
28_W45_P0.8190.93
164_I202_F0.8180.93
114_I136_I0.8170.93
159_P164_I0.8170.93
48_A67_A0.8130.93
29_I88_W0.8100.93
155_I193_L0.8030.92
79_Q83_D0.8030.92
105_G110_F0.7990.92
27_L94_P0.7990.92
40_D56_T0.7960.92
102_I129_L0.7950.92
164_I167_L0.7950.92
139_F142_G0.7910.92
37_T40_D0.7900.92
112_G189_I0.7880.91
89_A94_P0.7840.91
67_A70_L0.7760.91
115_Q184_G0.7750.91
164_I207_L0.7740.91
12_K18_L0.7720.91
86_L120_G0.7600.90
175_P194_I0.7520.89
103_T174_Y0.7490.89
168_T203_A0.7480.89
35_T39_L0.7460.89
170_T201_S0.7460.89
118_Y138_M0.7440.89
30_G36_V0.7430.89
83_D116_L0.7430.89
87_D119_K0.7390.89
73_Y79_Q0.7380.89
167_L202_F0.7360.88
159_P183_L0.7350.88
147_E196_S0.7310.88
160_F208_I0.7240.88
148_K153_R0.7180.87
156_S181_I0.7180.87
148_K193_L0.7170.87
44_I68_P0.7160.87
106_R154_Y0.7150.87
62_R66_A0.7130.87
159_P184_G0.7110.87
35_T63_I0.7100.87
68_P71_H0.7090.87
149_D153_R0.7050.86
128_R137_Q0.7050.86
31_V120_G0.7000.86
38_L45_P0.6990.86
90_L97_I0.6990.86
45_P67_A0.6840.85
83_D187_L0.6790.84
114_I138_M0.6760.84
33_K59_E0.6730.84
155_I209_M0.6710.83
7_V34_G0.6700.83
58_Q61_R0.6690.83
52_F154_Y0.6670.83
101_G104_G0.6650.83
47_E69_A0.6650.83
30_G34_G0.6580.82
157_F172_F0.6550.82
73_Y77_K0.6540.82
35_T48_A0.6540.82
129_L136_I0.6530.82
62_R65_K0.6490.81
103_T107_A0.6450.81
30_G33_K0.6450.81
112_G188_C0.6450.81
186_T189_I0.6440.81
76_E174_Y0.6430.81
6_I83_D0.6400.80
9_G34_G0.6370.80
60_R72_V0.6370.80
155_I169_L0.6370.80
167_L179_C0.6340.80
100_F130_I0.6330.80
156_S209_M0.6330.80
74_Q79_Q0.6310.80
113_N116_L0.6300.79
165_H203_A0.6290.79
7_V12_K0.6260.79
7_V100_F0.6250.79
161_S206_I0.6230.79
167_L181_I0.6220.79
83_D87_D0.6220.79
9_G82_L0.6210.78
97_I127_I0.6180.78
115_Q183_L0.6120.77
4_I27_L0.6100.77
112_G190_S0.6070.77
175_P195_H0.6040.77
63_I67_A0.6020.76
179_C188_C0.5990.76
28_W38_L0.5970.76
157_F200_F0.5940.75
79_Q185_S0.5940.75
167_L188_C0.5930.75
168_T201_S0.5870.74
38_L41_A0.5850.74
107_A134_N0.5820.74
34_G77_K0.5810.74
107_A114_I0.5800.74
32_D109_H0.5790.73
77_K189_I0.5790.73
83_D186_T0.5780.73
99_I127_I0.5780.73
31_V101_G0.5740.73
34_G190_S0.5740.73
82_L112_G0.5730.73
166_E202_F0.5720.73
115_Q185_S0.5700.72
181_I187_L0.5690.72
115_Q158_I0.5660.72
167_L176_L0.5660.72
33_K52_F0.5640.72
39_L42_G0.5550.70
169_L198_G0.5540.70
74_Q84_L0.5530.70
77_K82_L0.5500.70
166_E204_K0.5500.70
159_P179_C0.5480.69
144_Y209_M0.5470.69
155_I167_L0.5460.69
42_G203_A0.5450.69
33_K37_T0.5430.69
32_D50_G0.5420.69
5_N22_T0.5410.68
44_I69_A0.5390.68
118_Y122_K0.5370.68
35_T60_R0.5310.67
77_K205_G0.5310.67
161_S183_L0.5310.67
163_D182_T0.5300.67
103_T129_L0.5270.66
52_F59_E0.5230.66
111_L114_I0.5210.66
120_G125_I0.5190.65
82_L186_T0.5190.65
112_G158_I0.5190.65
91_E194_I0.5170.65
87_D186_T0.5140.64
14_L40_D0.5130.64
21_Y130_I0.5130.64
55_I67_A0.5130.64
28_W100_F0.5130.64
44_I67_A0.5130.64
203_A207_L0.5120.64
49_F74_Q0.5120.64
122_K140_P0.5090.64
124_N127_I0.5080.64
82_L179_C0.5060.63
44_I66_A0.5060.63
141_P202_F0.5050.63
39_L67_A0.5040.63
180_H203_A0.5040.63
83_D86_L0.5040.63
118_Y184_G0.5000.62
181_I186_T0.5000.62
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3lm8A 2 0.9953 100 0.09 Contact Map
3k94A 2 0.986 100 0.107 Contact Map
3melA 2 0.9813 100 0.124 Contact Map
3l8mA 5 0.9579 100 0.133 Contact Map
2g9zA 2 0.9813 100 0.14 Contact Map
3s4yA 2 0.9766 100 0.141 Contact Map
3cq9A 2 0.972 100 0.143 Contact Map
3ihkA 3 0.9533 100 0.151 Contact Map
1ig0A 2 0.9953 100 0.153 Contact Map
2omkA 2 0.9299 100 0.216 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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