GREMLIN Database
AGAL - Alpha-galactosidase
UniProt: O34645 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 432 (412)
Sequences: 1744 (1031)
Seq/√Len: 50.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
70_K136_E5.6161.00
78_Y319_E4.0951.00
73_L138_C3.9371.00
69_R133_D3.7501.00
101_R419_D3.4031.00
189_G209_D3.3481.00
134_M143_F3.3371.00
134_M163_I3.1631.00
352_D357_T3.1401.00
47_Q65_S3.1371.00
391_K418_D3.1221.00
205_E212_D3.0781.00
317_A324_F3.0341.00
395_Y417_C3.0341.00
68_D71_L2.9851.00
70_K133_D2.9171.00
32_E62_A2.8251.00
32_E64_N2.7831.00
328_G351_A2.7781.00
104_L416_M2.7391.00
337_T363_E2.5831.00
211_T216_E2.5521.00
135_E161_T2.5411.00
47_Q51_E2.5301.00
364_L372_N2.4491.00
186_E189_G2.4271.00
166_I356_I2.3961.00
335_L345_V2.3771.00
3_K34_A2.3521.00
394_V417_C2.3031.00
208_K213_L2.2991.00
85_V126_V2.2611.00
132_K135_E2.2611.00
34_A64_N2.2591.00
44_Q48_L2.2541.00
388_T393_A2.2531.00
275_L283_V2.2351.00
333_T341_S2.2311.00
134_M138_C2.1961.00
117_I382_T2.1561.00
328_G349_C2.1511.00
135_E162_N2.1331.00
93_V279_P2.1321.00
159_R384_E2.1221.00
129_D132_K2.1151.00
236_E259_F2.0961.00
102_Y415_S2.0961.00
131_A157_M2.0601.00
338_N369_A2.0311.00
104_L114_I2.0251.00
339_L372_N1.9831.00
105_R268_I1.9590.99
233_V237_L1.9450.99
124_I156_A1.9170.99
89_K282_C1.8950.99
189_G208_K1.8880.99
70_K137_M1.8840.99
102_Y419_D1.8760.99
233_V236_E1.8710.99
283_V287_E1.8700.99
27_A316_E1.8510.99
142_W164_K1.8360.99
213_L216_E1.8220.99
44_Q47_Q1.8150.99
158_L331_L1.7940.99
54_R63_I1.7940.99
334_G341_S1.7910.99
124_I152_T1.7850.99
18_V33_F1.7810.99
202_W260_I1.7540.99
128_F156_A1.7420.99
71_L74_Q1.7370.99
316_E320_T1.7210.99
350_V359_C1.7050.99
94_I423_A1.7010.99
384_E388_T1.6820.98
390_K393_A1.6590.98
34_A66_Y1.6580.98
234_R237_L1.6580.98
140_D164_K1.6260.98
384_E393_A1.6130.98
318_M321_N1.6080.98
183_L217_I1.5830.98
123_T126_V1.5700.98
219_R223_E1.5510.97
48_L51_E1.5420.97
393_A396_Q1.5400.97
78_Y318_M1.5300.97
3_K32_E1.5290.97
338_N362_G1.5270.97
216_E220_R1.5240.97
28_L315_I1.5210.97
64_N71_L1.5140.97
114_I404_T1.5140.97
323_P352_D1.5070.97
193_R205_E1.4960.97
45_E293_R1.4920.97
325_T350_V1.4920.97
352_D355_K1.4910.97
93_V278_Y1.4890.97
89_K93_V1.4860.97
77_G140_D1.4830.96
5_T76_A1.4810.96
117_I378_T1.4800.96
40_P44_Q1.4780.96
127_L157_M1.4740.96
10_G37_D1.4630.96
287_E303_T1.4530.96
131_A134_M1.4530.96
237_L240_K1.4350.96
112_V375_N1.4300.96
337_T362_G1.4210.96
386_A389_R1.4130.95
157_M165_T1.4110.95
352_D356_I1.3870.95
54_R61_V1.3590.94
369_A373_R1.3530.94
385_A394_V1.3490.94
185_M208_K1.3340.94
237_L241_F1.3110.93
290_E293_R1.3100.93
5_T34_A1.2970.93
104_L409_S1.2920.93
102_Y416_M1.2920.93
79_V134_M1.2810.92
326_F351_A1.2780.92
124_I386_A1.2770.92
306_R312_S1.2770.92
66_Y72_A1.2700.92
266_E270_E1.2690.92
416_M420_L1.2600.92
278_Y282_C1.2490.91
31_F62_A1.2470.91
98_I423_A1.2410.91
354_K357_T1.2390.91
39_D87_G1.2310.91
79_V141_A1.2300.91
5_T73_L1.2290.91
74_Q137_M1.2280.91
128_F387_V1.2260.90
191_E207_K1.2110.90
398_A420_L1.2060.90
125_P386_A1.2030.90
337_T341_S1.1970.89
93_V97_E1.1970.89
81_N127_L1.1900.89
307_S311_G1.1890.89
94_I424_H1.1750.89
422_A425_G1.1690.88
27_A322_E1.1630.88
279_P282_C1.1540.88
51_E55_D1.1530.88
44_Q65_S1.1520.88
75_N140_D1.1520.88
4_I28_L1.1460.87
299_N302_L1.1350.87
195_A204_L1.1270.86
131_A163_I1.1210.86
366_E377_N1.1200.86
41_K293_R1.1110.86
236_E240_K1.1080.85
36_Y72_A1.1070.85
338_N373_R1.1030.85
336_I345_V1.1020.85
84_Q92_T1.1000.85
335_L347_V1.0950.85
195_A364_L1.0940.85
337_T360_F1.0920.85
99_P102_Y1.0910.84
391_K417_C1.0880.84
200_M375_N1.0880.84
321_N353_R1.0850.84
267_L271_L1.0770.84
215_P242_G1.0760.84
378_T398_A1.0750.84
263_N406_A1.0730.83
325_T359_C1.0720.83
127_L153_L1.0620.83
216_E219_R1.0590.83
269_S273_I1.0590.83
73_L137_M1.0530.82
99_P420_L1.0500.82
2_K31_F1.0480.82
330_V349_C1.0450.82
95_D122_R1.0410.82
135_E163_I1.0400.82
294_D299_N1.0380.81
50_L63_I1.0350.81
176_T192_E1.0310.81
7_I143_F1.0280.81
350_V357_T1.0250.81
233_V240_K1.0220.80
104_L405_S1.0190.80
284_K288_N1.0190.80
263_N409_S1.0190.80
133_D137_M1.0180.80
405_S409_S1.0140.80
394_V421_F1.0080.79
222_K239_D1.0060.79
367_Q396_Q1.0050.79
160_Y387_V1.0040.79
307_S310_Y1.0000.79
391_K411_K0.9990.79
116_G122_R0.9990.79
225_Q228_K0.9970.79
118_F421_F0.9940.78
235_F239_D0.9910.78
43_L65_S0.9880.78
92_T96_F0.9880.78
151_A379_Q0.9800.77
7_I130_I0.9780.77
194_I203_L0.9760.77
205_E214_Y0.9720.77
325_T348_T0.9710.77
89_K94_I0.9670.77
93_V282_C0.9660.76
111_T116_G0.9660.76
83_I150_M0.9640.76
40_P67_D0.9570.76
212_D215_P0.9520.75
233_V241_F0.9470.75
218_K238_M0.9470.75
200_M371_L0.9400.74
307_S312_S0.9370.74
83_I130_I0.9350.74
155_G380_L0.9340.74
153_L156_A0.9280.74
291_K294_D0.9260.73
169_C199_H0.9260.73
234_R238_M0.9250.73
174_V178_D0.9240.73
106_Q115_G0.9200.73
391_K421_F0.9190.73
132_K136_E0.9150.72
232_M237_L0.9100.72
338_N345_V0.9040.72
194_I201_A0.9040.72
120_S344_V0.9010.71
126_V129_D0.8990.71
176_T203_L0.8980.71
398_A410_M0.8950.71
5_T36_Y0.8950.71
171_S254_E0.8860.70
227_T230_H0.8820.70
117_I398_A0.8770.69
97_E279_P0.8770.69
343_A376_I0.8720.69
236_E411_K0.8710.69
271_L274_P0.8680.68
101_R271_L0.8650.68
375_N401_D0.8620.68
191_E325_T0.8600.68
421_F425_G0.8600.68
336_I362_G0.8590.68
330_V351_A0.8570.67
112_V401_D0.8560.67
76_A138_C0.8530.67
313_R324_F0.8520.67
129_D133_D0.8520.67
274_P277_E0.8520.67
155_G159_R0.8520.67
166_I318_M0.8460.66
233_V238_M0.8450.66
398_A416_M0.8380.66
186_E208_K0.8320.65
142_W318_M0.8320.65
9_A410_M0.8310.65
349_C358_P0.8290.65
118_F424_H0.8270.65
343_A380_L0.8270.65
391_K395_Y0.8260.65
236_E239_D0.8250.64
389_R429_P0.8210.64
132_K160_Y0.8190.64
12_T289_W0.8150.63
99_P416_M0.8140.63
150_M172_V0.8110.63
120_S124_I0.8090.63
159_R380_L0.8090.63
220_R224_K0.8020.62
78_Y315_I0.7990.62
394_V429_P0.7940.61
415_S418_D0.7930.61
13_I82_A0.7920.61
370_A377_N0.7910.61
56_R298_N0.7900.61
84_Q96_F0.7890.61
2_K319_E0.7880.61
73_L79_V0.7840.60
176_T187_H0.7820.60
144_L315_I0.7710.59
338_N363_E0.7690.59
40_P219_R0.7690.59
420_L423_A0.7670.59
54_R59_P0.7640.58
99_P115_G0.7630.58
327_G348_T0.7630.58
215_P219_R0.7620.58
351_A356_I0.7600.58
238_M241_F0.7590.58
112_V259_F0.7560.58
290_E294_D0.7560.58
6_F80_I0.7550.58
193_R361_A0.7530.57
316_E322_E0.7500.57
180_F185_M0.7490.57
85_V123_T0.7490.57
232_M236_E0.7470.57
155_G376_I0.7430.56
415_S419_D0.7400.56
51_E62_A0.7390.56
340_P343_A0.7390.56
173_Q314_I0.7390.56
201_A246_T0.7390.56
234_R240_K0.7380.56
306_R311_G0.7380.56
41_K341_S0.7350.56
143_F157_M0.7350.56
158_L165_T0.7330.55
114_I398_A0.7330.55
404_T410_M0.7300.55
177_K187_H0.7300.55
374_T400_L0.7290.55
76_A79_V0.7280.55
399_M410_M0.7280.55
4_I21_D0.7270.55
175_C201_A0.7270.55
348_T365_P0.7210.54
237_L245_V0.7210.54
377_N380_L0.7190.54
308_K311_G0.7180.54
179_L206_V0.7160.54
106_Q109_A0.7160.54
95_D116_G0.7160.54
381_M396_Q0.7160.54
69_R73_L0.7140.53
66_Y71_L0.7120.53
35_L50_L0.7120.53
146_Y311_G0.7100.53
154_T167_G0.7090.53
51_E71_L0.7060.53
46_S65_S0.7060.53
234_R241_F0.7040.52
170_H307_S0.7000.52
50_L54_R0.6990.52
395_Y410_M0.6990.52
69_R129_D0.6990.52
146_Y151_A0.6980.52
112_V200_M0.6970.52
33_F61_V0.6970.52
184_G239_D0.6960.52
191_E205_E0.6950.51
111_T152_T0.6950.51
118_F420_L0.6950.51
118_F138_C0.6950.51
200_M401_D0.6940.51
14_F82_A0.6910.51
395_Y398_A0.6900.51
119_R259_F0.6880.51
48_L65_S0.6850.50
44_Q357_T0.6850.50
111_T259_F0.6830.50
92_T277_E0.6830.50
106_Q379_Q0.6810.50
100_K268_I0.6800.50
143_F165_T0.6800.50
157_M163_I0.6790.50
195_A361_A0.6780.50
416_M419_D0.6770.50
46_S50_L0.6760.49
7_I73_L0.6750.49
117_I394_V0.6740.49
169_C198_N0.6730.49
170_H309_E0.6730.49
202_W396_Q0.6720.49
140_D298_N0.6710.49
120_S156_A0.6690.49
94_I98_I0.6680.49
182_A217_I0.6680.49
99_P118_F0.6670.49
171_S246_T0.6670.49
367_Q372_N0.6660.48
382_T398_A0.6630.48
285_Q289_W0.6630.48
20_G154_T0.6630.48
317_A326_F0.6610.48
286_I290_E0.6600.48
11_S16_K0.6590.48
130_I143_F0.6590.48
194_I327_G0.6560.47
148_N198_N0.6540.47
426_D429_P0.6530.47
246_T256_H0.6520.47
192_E203_L0.6520.47
98_I419_D0.6510.47
96_F106_Q0.6510.47
399_M407_E0.6500.47
291_K303_T0.6490.47
110_D119_R0.6490.47
214_Y218_K0.6490.47
71_L422_A0.6480.47
179_L217_I0.6480.47
13_I16_K0.6470.46
156_A386_A0.6470.46
188_D211_T0.6460.46
41_K334_G0.6460.46
33_F418_D0.6460.46
219_R239_D0.6450.46
204_L243_Y0.6440.46
277_E281_R0.6430.46
304_H310_Y0.6430.46
185_M189_G0.6430.46
73_L133_D0.6420.46
9_A47_Q0.6410.46
53_L141_A0.6390.46
55_D190_I0.6380.46
347_V361_A0.6380.46
160_Y388_T0.6360.45
114_I416_M0.6350.45
268_I271_L0.6340.45
64_N136_E0.6330.45
41_K44_Q0.6330.45
22_C317_A0.6330.45
96_F110_D0.6330.45
208_K211_T0.6330.45
285_Q379_Q0.6320.45
366_E373_R0.6310.45
8_G11_S0.6310.45
144_L166_I0.6290.45
123_T386_A0.6290.45
74_Q293_R0.6280.45
112_V378_T0.6280.45
106_Q150_M0.6210.44
84_Q119_R0.6200.44
417_C421_F0.6200.44
43_L67_D0.6190.44
376_I379_Q0.6180.44
35_L63_I0.6170.43
14_F17_N0.6100.43
4_I315_I0.6080.43
151_A375_N0.6080.43
33_F211_T0.6080.43
207_K212_D0.6070.42
151_A200_M0.6060.42
338_N364_L0.6060.42
13_I88_Y0.6040.42
323_P353_R0.6040.42
386_A428_L0.6010.42
341_S363_E0.6010.42
228_K231_D0.6000.42
269_S272_Q0.5990.42
282_C286_I0.5980.41
176_T206_V0.5960.41
7_I38_I0.5950.41
165_T344_V0.5950.41
112_V152_T0.5950.41
106_Q110_D0.5940.41
294_D300_K0.5940.41
226_K230_H0.5940.41
89_K426_D0.5930.41
84_Q379_Q0.5910.41
79_V138_C0.5890.41
96_F109_A0.5890.41
345_V348_T0.5880.40
171_S201_A0.5880.40
4_I103_G0.5870.40
2_K77_G0.5870.40
33_F71_L0.5870.40
278_Y281_R0.5860.40
372_N396_Q0.5840.40
378_T382_T0.5830.40
333_T360_F0.5830.40
285_Q288_N0.5810.40
47_Q63_I0.5810.40
283_V286_I0.5810.40
54_R60_S0.5810.40
27_A315_I0.5800.40
67_D290_E0.5800.40
148_N199_H0.5800.40
316_E349_C0.5790.40
236_E241_F0.5760.39
100_K271_L0.5750.39
171_S175_C0.5730.39
398_A404_T0.5720.39
51_E140_D0.5690.39
111_T122_R0.5690.39
335_L342_K0.5690.39
102_Y271_L0.5690.39
267_L270_E0.5690.39
347_V358_P0.5680.39
10_G38_I0.5680.39
204_L363_E0.5680.39
381_M397_A0.5670.38
329_N344_V0.5660.38
362_G369_A0.5650.38
44_Q269_S0.5640.38
75_N298_N0.5640.38
342_K386_A0.5620.38
152_T406_A0.5620.38
55_D186_E0.5620.38
100_K277_E0.5620.38
92_T111_T0.5590.38
371_L381_M0.5590.38
204_L365_P0.5580.38
117_I120_S0.5580.38
148_N169_C0.5580.38
261_K407_E0.5570.37
323_P350_V0.5570.37
36_Y73_L0.5570.37
234_R411_K0.5560.37
177_K181_K0.5560.37
25_T60_S0.5540.37
123_T153_L0.5530.37
183_L208_K0.5520.37
113_G122_R0.5510.37
321_N352_D0.5500.37
281_R424_H0.5500.37
304_H307_S0.5500.37
6_F17_N0.5470.36
172_V327_G0.5460.36
132_K272_Q0.5450.36
95_D113_G0.5450.36
21_D170_H0.5430.36
51_E239_D0.5420.36
49_M297_V0.5420.36
408_L416_M0.5410.36
318_M322_E0.5410.36
96_F277_E0.5400.36
142_W166_I0.5400.36
16_K20_G0.5400.36
132_K161_T0.5400.36
17_N96_F0.5390.36
16_K82_A0.5390.36
195_A365_P0.5390.36
44_Q75_N0.5390.36
5_T64_N0.5380.36
147_T427_W0.5380.36
73_L112_V0.5380.36
119_R123_T0.5380.36
290_E425_G0.5370.36
284_K287_E0.5370.36
5_T74_Q0.5360.35
80_I315_I0.5360.35
84_Q106_Q0.5350.35
98_I416_M0.5350.35
73_L130_I0.5350.35
241_F245_V0.5340.35
365_P368_L0.5340.35
210_G429_P0.5330.35
266_E384_E0.5330.35
193_R204_L0.5330.35
25_T317_A0.5330.35
392_E396_Q0.5320.35
21_D307_S0.5310.35
118_F373_R0.5310.35
167_G402_P0.5310.35
163_I345_V0.5310.35
92_T424_H0.5310.35
7_I69_R0.5290.35
182_A235_F0.5290.35
306_R310_Y0.5290.35
81_N147_T0.5280.35
40_P136_E0.5280.35
51_E64_N0.5270.35
96_F379_Q0.5270.35
373_R377_N0.5260.35
339_L383_I0.5250.34
183_L216_E0.5240.34
333_T358_P0.5240.34
53_L57_Y0.5240.34
152_T321_N0.5230.34
14_F21_D0.5230.34
191_E323_P0.5230.34
51_E183_L0.5220.34
3_K76_A0.5220.34
369_A380_L0.5210.34
180_F190_I0.5200.34
7_I36_Y0.5190.34
147_T270_E0.5190.34
143_F163_I0.5180.34
81_N134_M0.5170.34
190_I208_K0.5170.34
157_M162_N0.5170.34
289_W292_M0.5170.34
96_F111_T0.5170.34
180_F202_W0.5170.34
81_N165_T0.5160.34
221_A239_D0.5160.34
381_M400_L0.5160.34
293_R296_I0.5150.33
50_L82_A0.5140.33
13_I84_Q0.5140.33
81_N143_F0.5120.33
158_L329_N0.5110.33
135_E157_M0.5100.33
62_A101_R0.5100.33
184_G211_T0.5100.33
6_F87_G0.5090.33
213_L218_K0.5070.33
316_E324_F0.5070.33
238_M245_V0.5070.33
375_N379_Q0.5060.33
42_R255_Y0.5060.33
138_C163_I0.5060.33
84_Q259_F0.5050.33
340_P380_L0.5050.33
33_F174_V0.5050.33
378_T401_D0.5050.33
71_L226_K0.5040.32
16_K307_S0.5030.32
111_T119_R0.5020.32
113_G116_G0.5020.32
306_R309_E0.5020.32
178_D231_D0.5020.32
103_G273_I0.5010.32
406_A410_M0.5010.32
217_I244_Y0.5000.32
94_I101_R0.5000.32
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1obbA 2 0.9653 100 0.352 Contact Map
1up7A 3 0.9468 100 0.36 Contact Map
1u8xX 1 0.956 100 0.364 Contact Map
1s6yA 1 0.9306 100 0.364 Contact Map
3fefA 3 0.9444 100 0.397 Contact Map
3u95A 2 0.9769 100 0.413 Contact Map
1pzgA 4 0.7222 100 0.716 Contact Map
4rorA 4 0.7222 100 0.721 Contact Map
3p7mA 4 0.6898 100 0.726 Contact Map
2hjrA 4 0.6944 100 0.728 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0127 seconds.