GREMLIN Database
SIRB - Sirohydrochlorin ferrochelatase
UniProt: O34632 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 261 (231)
Sequences: 1693 (1115)
Seq/√Len: 73.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
12_S17_A4.5541.00
115_H229_H3.7601.00
7_Y25_L3.5631.00
31_H232_N3.4631.00
78_K138_D3.3731.00
63_H96_R3.2451.00
5_I238_V3.1701.00
115_H227_H3.0431.00
22_A41_F2.7531.00
31_H231_K2.7201.00
48_T51_T2.6701.00
111_K155_M2.6361.00
71_L193_Y2.6231.00
113_V131_L2.5361.00
6_L52_G2.5141.00
32_I239_R2.3821.00
38_E55_A2.3731.00
6_L64_I2.3031.00
28_C234_F2.2141.00
8_V66_V2.2131.00
32_I235_L2.1381.00
126_N186_I2.1121.00
27_G231_K2.0891.00
63_H98_S2.0841.00
76_H80_D2.0281.00
127_A187_T1.9791.00
32_I35_P1.9391.00
108_E236_N1.9351.00
3_Q238_V1.9081.00
78_K140_D1.8941.00
66_V85_I1.8641.00
27_G226_F1.7891.00
74_A200_L1.7741.00
10_H76_H1.7561.00
172_N175_E1.7381.00
35_P235_L1.7311.00
27_G228_P1.7191.00
53_F97_I1.6701.00
168_A171_P1.6571.00
13_R44_L1.6341.00
233_A236_N1.6050.99
3_Q63_H1.6010.99
85_I99_Y1.5990.99
146_T165_F1.5860.99
101_K240_E1.5750.99
235_L239_R1.5360.99
76_H82_P1.5270.99
112_A227_H1.5150.99
108_E232_N1.5000.99
170_G175_E1.4820.99
15_K19_Q1.4640.99
192_P201_M1.4180.99
121_G124_Y1.4040.99
130_V176_V1.3970.98
26_E29_K1.3960.98
106_D236_N1.3840.98
14_V202_N1.3590.98
49_I81_I1.3490.98
25_L39_I1.3420.98
40_S52_G1.3350.98
132_I164_C1.3230.98
4_A61_A1.3060.98
230_V233_A1.3050.98
9_G133_G1.3000.98
12_S44_L1.2920.97
208_V212_K1.2850.97
111_K229_H1.2830.97
162_I179_E1.2820.97
16_K202_N1.2590.97
134_R200_L1.2560.97
164_C176_V1.2540.97
117_M189_F1.2180.96
19_Q22_A1.2170.96
110_V151_L1.2140.96
73_T144_D1.2110.96
192_P221_S1.2030.96
75_A134_R1.2010.96
114_Y118_K1.1980.96
199_M203_E1.1860.96
156_V159_K1.1760.96
10_H80_D1.1760.96
43_E75_A1.1650.95
65_A100_G1.1650.95
12_S42_L1.1610.95
128_R180_L1.1550.95
76_H200_L1.1510.95
4_A36_V1.1500.95
114_Y156_V1.1370.95
194_L201_M1.1270.94
166_L194_L1.1240.94
116_R119_D1.1070.94
113_V191_V1.1070.94
35_P238_V1.1040.94
65_A242_A1.0990.94
149_A163_P1.0960.93
13_R199_M1.0950.93
74_A136_S1.0780.93
43_E76_H1.0750.93
7_Y37_Q1.0730.93
165_F171_P1.0700.93
7_Y21_A1.0680.93
86_V89_A1.0670.93
236_N240_E1.0560.92
53_F88_V1.0550.92
49_I84_E1.0490.92
6_L40_S1.0450.92
134_R196_F1.0330.91
52_G192_P1.0260.91
67_V100_G1.0120.90
146_T150_N1.0100.90
23_A26_E1.0070.90
10_H43_E1.0040.90
105_I193_Y1.0030.90
107_E151_L1.0020.90
134_R199_M1.0020.90
65_A238_V1.0020.90
65_A241_T0.9920.90
175_E178_S0.9900.89
28_C235_L0.9890.89
70_L168_A0.9880.89
131_L161_V0.9880.89
74_A77_A0.9830.89
50_E84_E0.9660.88
187_T219_Y0.9590.88
117_M191_V0.9590.88
29_K37_Q0.9590.88
43_E134_R0.9550.88
117_M129_V0.9550.88
113_V193_Y0.9490.87
6_L56_C0.9400.87
149_A161_V0.9390.87
34_V54_E0.9360.87
70_L77_A0.9250.86
142_K165_F0.9220.86
20_E197_T0.9150.85
71_L76_H0.9150.85
38_E59_Q0.9140.85
42_L74_A0.9100.85
30_A33_S0.9070.85
65_A98_S0.9010.84
114_Y155_M0.8930.84
16_K19_Q0.8880.84
13_R200_L0.8860.83
144_D151_L0.8850.83
18_Q45_Q0.8820.83
43_E80_D0.8810.83
177_F188_T0.8780.83
54_E58_K0.8760.83
6_L192_P0.8650.82
118_K124_Y0.8640.82
110_V148_I0.8610.82
36_V59_Q0.8590.81
70_L74_A0.8580.81
21_A39_I0.8560.81
100_G234_F0.8560.81
189_F221_S0.8470.81
53_F64_I0.8390.80
10_H200_L0.8360.80
36_V61_A0.8360.80
130_V164_C0.8250.79
71_L195_L0.8180.78
49_I53_F0.8180.78
28_C37_Q0.8170.78
201_M223_Y0.8080.77
195_L230_V0.8080.77
70_L80_D0.8040.77
70_L79_H0.8030.77
130_V177_F0.8030.77
42_L193_Y0.7990.77
22_A39_I0.7900.76
136_S198_G0.7900.76
197_T202_N0.7860.75
164_C180_L0.7850.75
137_S195_L0.7850.75
128_R185_G0.7840.75
128_R188_T0.7840.75
75_A137_S0.7760.74
8_V159_K0.7750.74
77_A102_P0.7680.74
44_L74_A0.7620.73
70_L200_L0.7580.73
75_A196_F0.7560.73
144_D147_G0.7490.72
176_V180_L0.7460.72
109_V230_V0.7390.71
10_H82_P0.7380.71
43_E132_I0.7370.71
46_E83_E0.7370.71
25_L29_K0.7340.70
9_G21_A0.7330.70
194_L200_L0.7330.70
64_I97_I0.7190.69
20_E196_F0.7170.69
162_I183_D0.7170.69
142_K169_C0.7170.69
143_R147_G0.7160.68
50_E87_R0.7150.68
172_N176_V0.7140.68
129_V161_V0.7140.68
127_A189_F0.7080.68
73_T78_K0.7060.67
137_S165_F0.7030.67
8_V177_F0.7020.67
151_L154_E0.7020.67
178_S182_K0.7010.67
112_A230_V0.7010.67
221_S225_G0.7010.67
73_T166_L0.7000.67
18_Q226_F0.6980.66
75_A79_H0.6960.66
81_I85_I0.6940.66
132_I204_I0.6920.66
51_T55_A0.6920.66
3_Q65_A0.6910.66
20_E225_G0.6890.65
8_V40_S0.6890.65
12_S82_P0.6880.65
74_A198_G0.6870.65
82_P200_L0.6830.65
134_R194_L0.6830.65
78_K204_I0.6790.64
20_E23_A0.6790.64
111_K114_Y0.6790.64
152_L156_V0.6770.64
76_H198_G0.6740.64
232_N235_L0.6720.64
23_A27_G0.6660.63
27_G30_A0.6650.63
41_F57_V0.6650.63
12_S194_L0.6650.63
167_T171_P0.6650.63
127_A223_Y0.6630.62
26_E96_R0.6630.62
125_E159_K0.6610.62
13_R82_P0.6590.62
80_D198_G0.6580.62
5_I65_A0.6540.61
174_K177_F0.6510.61
20_E223_Y0.6470.61
44_L200_L0.6470.61
140_D144_D0.6440.60
81_I201_M0.6430.60
167_T192_P0.6420.60
173_Y207_E0.6370.59
123_P126_N0.6360.59
148_I193_Y0.6320.59
228_P232_N0.6300.59
48_T172_N0.6300.59
235_L238_V0.6290.58
103_I237_R0.6290.58
209_Q212_K0.6280.58
42_L194_L0.6270.58
8_V13_R0.6260.58
196_F200_L0.6230.58
87_R90_S0.6210.57
174_K210_K0.6200.57
116_R227_H0.6160.57
230_V239_R0.6150.57
218_V222_S0.6120.56
21_A37_Q0.6100.56
43_E52_G0.6080.56
150_N153_Q0.6070.56
44_L130_V0.6070.56
233_A237_R0.6070.56
173_Y178_S0.6070.56
212_K219_Y0.6040.55
10_H70_L0.6010.55
194_L224_I0.5970.55
189_F233_A0.5960.54
120_I189_F0.5940.54
43_E49_I0.5920.54
28_C108_E0.5920.54
56_C61_A0.5900.54
201_M205_E0.5820.53
47_P76_H0.5800.52
189_F192_P0.5800.52
22_A26_E0.5770.52
98_S241_T0.5750.52
31_H235_L0.5750.52
69_L234_F0.5730.52
9_G41_F0.5730.52
50_E54_E0.5720.51
110_V114_Y0.5700.51
24_F130_V0.5690.51
120_I221_S0.5690.51
71_L196_F0.5660.51
134_R167_T0.5650.51
16_K233_A0.5640.50
117_M201_M0.5640.50
47_P80_D0.5610.50
58_K119_D0.5600.50
13_R40_S0.5590.50
111_K115_H0.5590.50
70_L76_H0.5570.50
140_D147_G0.5560.49
46_E51_T0.5550.49
77_A141_V0.5530.49
210_K219_Y0.5520.49
54_E59_Q0.5520.49
10_H198_G0.5490.49
7_Y168_A0.5470.48
21_A112_A0.5420.48
46_E49_I0.5410.48
212_K218_V0.5410.48
59_Q62_T0.5400.47
61_A95_V0.5390.47
194_L198_G0.5390.47
153_Q169_C0.5380.47
72_L196_F0.5380.47
101_K237_R0.5380.47
63_H183_D0.5370.47
10_H13_R0.5360.47
165_F169_C0.5350.47
238_V242_A0.5310.46
68_P198_G0.5300.46
103_I230_V0.5300.46
105_I134_R0.5300.46
76_H137_S0.5280.46
152_L227_H0.5250.46
97_I163_P0.5240.45
40_S48_T0.5230.45
9_G39_I0.5230.45
85_I97_I0.5220.45
147_G151_L0.5160.44
171_P196_F0.5150.44
86_V179_E0.5140.44
17_A185_G0.5140.44
67_V238_V0.5130.44
132_I173_Y0.5120.44
17_A195_L0.5120.44
134_R192_P0.5120.44
102_P137_S0.5110.44
89_A96_R0.5110.44
106_D193_Y0.5100.44
205_E222_S0.5090.44
6_L53_F0.5080.44
44_L72_L0.5070.43
75_A167_T0.5060.43
131_L177_F0.5050.43
131_L138_D0.5050.43
239_R242_A0.5020.43
154_E240_E0.5010.43
180_L185_G0.5010.43
43_E79_H0.5010.43
150_N154_E0.5000.43
44_L134_R0.5000.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2jh3A 2 0.9425 100 0.411 Contact Map
2xvyA 3 0.9234 100 0.428 Contact Map
4ccsA 1 0.8352 100 0.436 Contact Map
2xwpA 5 0.9042 100 0.463 Contact Map
1lbqA 2 0.9808 100 0.565 Contact Map
3hcnA 2 0.9923 100 0.567 Contact Map
2h1vA 1 0.9119 100 0.581 Contact Map
2xwsA 2 0.4521 99.9 0.645 Contact Map
1tjnA 4 0.4483 99.9 0.648 Contact Map
3lyhA 2 0.4636 99.9 0.672 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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