GREMLIN Database
YEFB - Uncharacterized protein YefB
UniProt: O34574 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 300 (271)
Sequences: 4266 (3890)
Seq/√Len: 236.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
75_P78_D4.7511.00
76_L80_L3.5471.00
106_V277_E3.0491.00
191_K226_D3.0401.00
106_V113_S2.9461.00
185_I234_L2.8641.00
108_K281_H2.8491.00
39_T67_E2.7691.00
192_L227_I2.7601.00
184_K233_T2.7431.00
39_T76_L2.6161.00
143_E147_T2.5371.00
188_I230_F2.3701.00
188_I234_L2.3641.00
112_R127_S2.3591.00
34_P39_T2.3131.00
28_R32_E2.2771.00
37_T76_L2.2571.00
104_T272_H2.2181.00
32_E86_L2.1921.00
189_N234_L2.1861.00
192_L230_F2.1851.00
207_A219_A2.1741.00
196_K227_I2.1031.00
195_K227_I2.0791.00
109_D137_S2.0261.00
268_P272_H2.0061.00
9_P12_L1.9731.00
9_P14_R1.9171.00
200_S222_I1.9011.00
9_P13_A1.8991.00
32_E87_I1.8831.00
189_N238_S1.8581.00
79_F83_Q1.8561.00
204_I219_A1.8261.00
101_F147_T1.8081.00
34_P65_I1.8061.00
212_S215_D1.8041.00
195_K223_T1.8041.00
221_N225_E1.7681.00
198_D208_N1.7331.00
227_I231_E1.7151.00
31_L82_V1.7081.00
34_P41_Q1.6771.00
40_L69_S1.6741.00
85_I89_R1.6451.00
34_P37_T1.6401.00
37_T79_F1.6251.00
123_Y143_E1.6151.00
67_E76_L1.6061.00
263_E266_L1.5941.00
199_K227_I1.5871.00
153_N156_K1.5571.00
216_Y220_I1.5521.00
195_K226_D1.5411.00
219_A222_I1.5361.00
72_P76_L1.5111.00
39_T68_K1.5081.00
80_L84_E1.5071.00
222_I226_D1.4831.00
279_E287_E1.4811.00
46_Y58_V1.4721.00
185_I189_N1.4671.00
185_I238_S1.4651.00
206_L213_K1.4611.00
229_N233_T1.4301.00
195_K230_F1.4281.00
60_L63_Q1.4281.00
105_A146_L1.4261.00
201_N206_L1.4121.00
58_V62_N1.4071.00
196_K231_E1.4031.00
199_K220_I1.3991.00
192_L234_L1.3861.00
234_L238_S1.3681.00
41_Q44_R1.3561.00
39_T65_I1.3481.00
231_E235_H1.3361.00
78_D82_V1.3221.00
142_S145_D1.3171.00
101_F143_E1.3041.00
23_H26_S1.2961.00
191_K194_D1.2901.00
78_D81_I1.2701.00
74_I82_V1.2661.00
41_Q65_I1.2611.00
200_S218_L1.2601.00
79_F82_V1.2571.00
198_D207_A1.2481.00
99_H123_Y1.2411.00
281_H285_T1.2391.00
233_T236_Q1.2371.00
59_N63_Q1.2271.00
105_A123_Y1.2121.00
226_D229_N1.2101.00
81_I84_E1.2031.00
185_I237_L1.2001.00
217_Q221_N1.1961.00
281_H287_E1.1951.00
191_K195_K1.1941.00
206_L216_Y1.1881.00
33_N36_Y1.1841.00
85_I88_T1.1831.00
198_D223_T1.1651.00
201_N209_G1.1621.00
181_I237_L1.1531.00
225_E229_N1.1501.00
16_K19_T1.1451.00
184_K187_E1.1351.00
188_I233_T1.1291.00
228_S231_E1.1271.00
15_K21_K1.1231.00
114_M280_V1.1171.00
181_I240_D1.1151.00
192_L231_E1.1111.00
36_Y82_V1.1021.00
169_E172_E1.0821.00
78_D85_I1.0791.00
31_L83_Q1.0771.00
108_K279_E1.0731.00
15_K22_W1.0691.00
193_N196_K1.0611.00
208_N213_K1.0531.00
109_D282_A1.0511.00
104_T271_L1.0501.00
203_V207_A1.0421.00
10_S13_A1.0401.00
186_I189_N1.0371.00
99_H102_T1.0351.00
194_D208_N1.0351.00
43_C58_V1.0231.00
119_N122_G1.0231.00
275_V278_I1.0171.00
114_M138_D1.0171.00
84_E88_T1.0111.00
37_T80_L1.0091.00
224_N228_S1.0061.00
228_S232_T1.0051.00
39_T69_S1.0051.00
202_L207_A1.0031.00
148_D152_T1.0011.00
14_R22_W0.9901.00
215_D224_N0.9901.00
213_K217_Q0.9891.00
194_D198_D0.9761.00
189_N193_N0.9621.00
40_L70_H0.9571.00
100_L271_L0.9561.00
101_F278_I0.9561.00
73_I82_V0.9551.00
201_N204_I0.9491.00
100_L123_Y0.9491.00
219_A223_T0.9471.00
210_V213_K0.9431.00
35_H70_H0.9361.00
220_I224_N0.9331.00
59_N62_N0.9321.00
127_S131_H0.9281.00
205_S210_V0.9281.00
195_K198_D0.9261.00
199_K223_T0.9261.00
25_S29_K0.9111.00
195_K199_K0.9101.00
181_I241_L0.9091.00
126_G130_K0.9091.00
193_N197_R0.9041.00
201_N205_S0.9011.00
233_T237_L0.8991.00
151_I155_N0.8971.00
101_F146_L0.8920.99
37_T72_P0.8920.99
9_P15_K0.8920.99
9_P16_K0.8890.99
100_L147_T0.8870.99
43_C63_Q0.8810.99
215_D220_I0.8750.99
105_A278_I0.8750.99
199_K224_N0.8750.99
271_L275_V0.8630.99
101_F150_V0.8630.99
10_S14_R0.8620.99
84_E87_I0.8600.99
232_T236_Q0.8590.99
210_V217_Q0.8560.99
230_F233_T0.8540.99
30_I36_Y0.8530.99
187_E191_K0.8520.99
198_D204_I0.8510.99
275_V288_I0.8460.99
44_R63_Q0.8390.99
263_E267_T0.8350.99
16_K20_D0.8340.99
36_Y74_I0.8310.99
199_K215_D0.8250.99
110_C125_C0.8240.99
182_E241_L0.8240.99
199_K203_V0.8230.99
235_H238_S0.8190.99
232_T235_H0.8130.99
37_T41_Q0.8110.99
66_I69_S0.8070.99
236_Q240_D0.8070.99
249_E252_D0.8050.99
107_C110_C0.8030.99
201_N207_A0.8000.99
150_V288_I0.7990.99
10_S22_W0.7970.99
37_T75_P0.7950.99
46_Y56_R0.7930.99
264_G267_T0.7920.99
202_L220_I0.7880.99
230_F234_L0.7860.99
188_I192_L0.7810.99
147_T151_I0.7790.99
111_R279_E0.7790.99
35_H40_L0.7780.99
186_I190_E0.7770.99
68_K71_E0.7760.99
68_K76_L0.7710.99
47_R55_R0.7700.98
256_S259_K0.7680.98
128_Y139_H0.7650.98
83_Q86_L0.7650.98
248_K252_D0.7580.98
189_N192_L0.7560.98
184_K237_L0.7550.98
256_S260_F0.7520.98
41_Q63_Q0.7480.98
270_M274_L0.7470.98
32_E83_Q0.7470.98
163_K166_Y0.7430.98
104_T275_V0.7380.98
192_L195_K0.7340.98
98_V102_T0.7340.98
40_L68_K0.7340.98
116_F120_R0.7300.98
203_V215_D0.7290.98
150_V153_N0.7230.98
203_V216_Y0.7210.98
204_I213_K0.7210.98
162_S165_N0.7210.98
202_L216_Y0.7190.98
263_E270_M0.7190.98
36_Y73_I0.7180.98
77_E81_I0.7170.98
154_L274_L0.7160.98
256_S261_M0.7150.98
191_K230_F0.7150.98
105_A114_M0.7140.98
115_H126_G0.7120.97
246_I249_E0.7080.97
43_C46_Y0.7080.97
205_S219_A0.7070.97
190_E194_D0.7060.97
185_I241_L0.7060.97
87_I90_K0.7050.97
218_L222_I0.7030.97
165_N168_K0.7010.97
17_N22_W0.6980.97
35_H71_E0.6970.97
10_S15_K0.6970.97
254_K258_D0.6970.97
152_T156_K0.6960.97
24_G28_R0.6940.97
131_H135_A0.6930.97
28_R86_L0.6920.97
181_I184_K0.6890.97
10_S16_K0.6860.97
40_L66_I0.6850.97
81_I85_I0.6830.97
107_C125_C0.6830.97
110_C136_C0.6830.97
206_L220_I0.6770.97
100_L264_G0.6750.97
188_I191_K0.6710.96
13_A16_K0.6680.96
58_V63_Q0.6580.96
225_E228_S0.6570.96
106_V279_E0.6570.96
22_W27_I0.6550.96
27_I30_I0.6550.96
34_P44_R0.6550.96
109_D139_H0.6510.96
182_E186_I0.6500.96
99_H116_F0.6490.96
212_S224_N0.6490.96
99_H143_E0.6460.96
40_L71_E0.6440.95
136_C139_H0.6430.95
116_F121_K0.6400.95
276_D279_E0.6360.95
126_G129_N0.6350.95
250_I257_L0.6340.95
117_K122_G0.6340.95
221_N224_N0.6340.95
215_D219_A0.6330.95
117_K120_R0.6320.95
123_Y147_T0.6310.95
127_S135_A0.6280.95
9_P17_N0.6270.95
124_V129_N0.6260.95
34_P60_L0.6250.95
196_K199_K0.6240.95
211_I216_Y0.6210.95
181_I185_I0.6210.95
144_I148_D0.6180.94
42_Q267_T0.6170.94
203_V206_L0.6160.94
128_Y136_C0.6150.94
205_S213_K0.6150.94
128_Y140_Y0.6130.94
268_P271_L0.6110.94
206_L211_I0.6110.94
251_I255_K0.6110.94
206_L210_V0.6090.94
234_L241_L0.6040.94
156_K159_L0.6030.94
26_S29_K0.6010.94
209_G212_S0.6000.93
123_Y268_P0.5990.93
231_E234_L0.5980.93
264_G268_P0.5980.93
31_L86_L0.5980.93
226_D230_F0.5970.93
118_K121_K0.5970.93
22_W68_K0.5950.93
86_L89_R0.5950.93
141_V280_V0.5930.93
194_D197_R0.5920.93
103_N113_S0.5890.93
213_K220_I0.5830.92
36_Y70_H0.5790.92
204_I223_T0.5760.92
214_E228_S0.5750.92
23_H29_K0.5730.92
192_L196_K0.5720.92
56_R59_N0.5720.92
24_G27_I0.5720.92
178_K241_L0.5710.92
197_R201_N0.5700.92
270_M273_L0.5700.92
249_E254_K0.5700.92
69_S72_P0.5690.92
168_K171_S0.5680.91
203_V219_A0.5660.91
253_F257_L0.5650.91
251_I254_K0.5650.91
197_R200_S0.5640.91
52_S55_R0.5640.91
16_K22_W0.5640.91
267_T270_M0.5630.91
247_E251_I0.5600.91
182_E185_I0.5590.91
14_R17_N0.5580.91
202_L206_L0.5570.91
210_V215_D0.5550.91
257_L260_F0.5540.90
139_H282_A0.5540.90
196_K218_L0.5520.90
168_K172_E0.5510.90
196_K200_S0.5510.90
141_V146_L0.5510.90
202_L223_T0.5480.90
205_S208_N0.5480.90
227_I230_F0.5480.90
100_L268_P0.5470.90
107_C136_C0.5470.90
217_Q220_I0.5440.90
211_I215_D0.5380.89
222_I229_N0.5330.89
260_F263_E0.5320.89
115_H129_N0.5310.89
39_T70_H0.5310.89
42_Q45_E0.5310.89
138_D282_A0.5310.89
47_R52_S0.5300.88
167_F170_L0.5290.88
12_L17_N0.5280.88
190_E193_N0.5260.88
74_I83_Q0.5250.88
184_K188_I0.5240.88
207_A223_T0.5230.88
98_V268_P0.5210.88
58_V61_E0.5180.87
45_E55_R0.5180.87
43_C64_I0.5150.87
155_N261_M0.5150.87
202_L219_A0.5150.87
114_M139_H0.5140.87
74_I78_D0.5130.87
53_K58_V0.5110.87
109_D128_Y0.5100.87
114_M127_S0.5100.87
253_F256_S0.5100.87
42_Q64_I0.5100.87
117_K121_K0.5100.87
104_T268_P0.5090.86
114_M123_Y0.5090.86
151_I267_T0.5070.86
37_T83_Q0.5070.86
252_D256_S0.5030.86
52_S56_R0.5030.86
17_N20_D0.5010.86
36_Y83_Q0.5010.86
200_S219_A0.5000.85
103_N106_V0.5000.85
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4kisA 2 0.86 100 0.348 Contact Map
4bqqA 2 0.3567 99.9 0.595 Contact Map
3h0gB 1 0.52 84.5 0.919 Contact Map
4y66B 1 0.56 83.6 0.92 Contact Map
4y66A 1 0.6433 78.1 0.925 Contact Map
4qiwB 1 0.49 70.2 0.929 Contact Map
1twfB 2 0.5167 69.7 0.929 Contact Map
4aybB 1 0.5033 68 0.93 Contact Map
4iloA 3 0.4767 66.7 0.931 Contact Map
3na7A 1 0.47 63.7 0.932 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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