PFKA - BACSU - GREMLIN Contacts

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PFKA - ATP-dependent 6-phosphofructokinase

UniProt:	O34529	Sequences: MKRIGVLTSGGDSPGMNAAVRAVVRKAIYHDVEVYGIYNGYAGLISGKIEKLELGSVGDIIHRGGTKLYTARCPEFKTVEGREKGIANLKKLGIEGLVVIGGDGSYMGAKKLTEHGFPCVGVPGTIDNDIPGTDFTIGFDTALNTVIDAIDKIRDTATSHERTYVIEVMGRHAGDIALWAGLAGGAESILIPEADYDMHEIIARLKRGHERGKKHSIIIVAEGVGSGVEFGKRIEEETNLETRVSVLGHIQRGGSPSAADRVLASRLGAYAVELLLEGKGGRCVGIQNNKLVDHDIIEILETKHTVEQNMYQLSKELSI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	319 (304)
Sequences:	4644 (2510)
Seq/√Len:	143.9

ECOLI - PFKA
BACSU - PFKA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
3_R	33_E	4.513	1.00
33_E	51_K	3.751	1.00
181_G	189_I	3.381	1.00
89_L	94_I	3.234	1.00
5_G	37_I	3.214	1.00
38_Y	50_E	3.211	1.00
99_V	105_S	3.049	1.00
282_R	293_D	2.959	1.00
87_A	91_K	2.878	1.00
110_K	114_E	2.835	1.00
140_D	257_S	2.641	1.00
53_E	56_S	2.613	1.00
47_G	91_K	2.558	1.00
177_A	219_I	2.484	1.00
36_G	68_L	2.431	1.00
47_G	92_L	2.426	1.00
274_L	293_D	2.392	1.00
146_V	176_I	2.357	1.00
279_K	282_R	2.318	1.00
233_R	237_E	2.214	1.00
232_K	236_E	2.185	1.00
38_Y	48_K	2.182	1.00
42_A	46_S	2.180	1.00
199_H	237_E	2.177	1.00
47_G	88_N	2.175	1.00
35_Y	51_K	2.153	1.00
49_I	92_L	2.153	1.00
150_I	219_I	2.151	1.00
88_N	91_K	2.148	1.00
140_D	144_N	2.057	1.00
78_T	81_G	2.054	1.00
32_V	276_L	2.052	1.00
206_K	210_E	2.050	1.00
35_Y	49_I	2.035	1.00
143_L	176_I	2.018	1.00
27_A	34_V	1.997	1.00
6_V	23_V	1.988	1.00
36_G	52_L	1.918	1.00
5_G	94_I	1.908	1.00
3_R	35_Y	1.901	1.00
270_Y	273_E	1.877	1.00
144_N	257_S	1.864	1.00
23_V	98_V	1.862	1.00
26_K	273_E	1.838	1.00
191_I	306_V	1.832	1.00
285_G	292_V	1.817	1.00
233_R	236_E	1.800	1.00
83_E	87_A	1.799	1.00
139_F	179_W	1.766	1.00
168_V	219_I	1.744	1.00
39_N	48_K	1.736	1.00
234_I	238_T	1.730	1.00
37_I	49_I	1.715	1.00
270_Y	291_L	1.706	1.00
279_K	293_D	1.694	1.00
282_R	295_D	1.680	1.00
146_V	180_A	1.674	1.00
287_Q	292_V	1.664	1.00
28_I	54_L	1.656	1.00
3_R	93_G	1.653	1.00
114_E	297_I	1.653	1.00
273_E	277_E	1.645	1.00
60_I	67_K	1.631	1.00
149_A	250_I	1.620	1.00
202_I	237_E	1.602	1.00
30_H	276_L	1.582	1.00
207_R	210_E	1.572	1.00
98_V	275_L	1.564	1.00
153_I	166_I	1.564	1.00
37_I	44_L	1.554	1.00
50_E	56_S	1.553	1.00
39_N	161_E	1.543	1.00
148_D	152_K	1.532	1.00
19_A	122_V	1.525	1.00
188_S	201_I	1.524	1.00
106_Y	121_G	1.493	1.00
39_N	69_Y	1.480	1.00
111_K	114_E	1.478	1.00
87_A	90_K	1.473	1.00
150_I	166_I	1.468	1.00
165_V	230_F	1.446	1.00
86_I	115_H	1.440	1.00
74_P	77_K	1.414	1.00
202_I	234_I	1.407	1.00
228_V	232_K	1.374	1.00
198_M	233_R	1.367	1.00
41_Y	108_G	1.351	1.00
205_L	216_S	1.329	1.00
150_I	184_G	1.326	1.00
96_G	275_L	1.315	1.00
137_I	264_A	1.314	1.00
84_K	87_A	1.297	1.00
23_V	272_V	1.289	1.00
295_D	298_E	1.275	1.00
231_G	244_V	1.270	1.00
21_R	265_S	1.260	1.00
203_A	207_R	1.259	1.00
287_Q	294_H	1.246	1.00
220_V	230_F	1.246	1.00
151_D	261_R	1.235	1.00
285_G	294_H	1.235	1.00
274_L	282_R	1.231	1.00
270_Y	274_L	1.225	1.00
35_Y	92_L	1.224	1.00
165_V	218_I	1.223	1.00
86_I	90_K	1.223	1.00
168_V	177_A	1.222	1.00
113_T	281_G	1.218	1.00
150_I	217_I	1.216	1.00
27_A	32_V	1.206	1.00
7_L	44_L	1.198	1.00
9_S	105_S	1.195	1.00
79_V	83_E	1.187	1.00
206_K	237_E	1.174	1.00
229_E	233_R	1.163	1.00
19_A	137_I	1.158	1.00
270_Y	293_D	1.146	0.99
110_K	297_I	1.143	0.99
45_I	73_C	1.139	0.99
23_V	268_G	1.133	0.99
24_V	57_V	1.129	0.99
6_V	100_I	1.128	0.99
245_S	250_I	1.120	0.99
187_E	213_K	1.113	0.99
197_D	200_E	1.112	0.99
200_E	203_A	1.109	0.99
120_V	282_R	1.104	0.99
283_C	294_H	1.093	0.99
8_T	16_M	1.091	0.99
109_A	119_C	1.089	0.99
99_V	109_A	1.076	0.99
142_A	176_I	1.072	0.99
7_L	37_I	1.071	0.99
187_E	208_G	1.069	0.99
274_L	284_V	1.068	0.99
89_L	97_L	1.066	0.99
104_G	108_G	1.063	0.99
26_K	269_A	1.060	0.99
150_I	180_A	1.058	0.99
106_Y	283_C	1.057	0.99
131_P	302_T	1.057	0.99
109_A	296_I	1.049	0.99
235_E	241_E	1.039	0.99
74_P	104_G	1.037	0.99
204_R	207_R	1.037	0.99
174_G	193_E	1.031	0.99
83_E	115_H	1.028	0.99
145_T	152_K	1.026	0.99
82_R	111_K	1.019	0.99
69_Y	161_E	1.018	0.99
4_I	27_A	1.013	0.99
229_E	232_K	1.011	0.99
207_R	211_R	1.007	0.99
24_V	28_I	1.006	0.99
24_V	34_V	0.994	0.99
232_K	235_E	0.994	0.99
7_L	99_V	0.991	0.98
35_Y	94_I	0.984	0.98
231_G	235_E	0.982	0.98
22_A	268_G	0.974	0.98
208_G	211_R	0.973	0.98
42_A	73_C	0.971	0.98
172_H	303_K	0.970	0.98
203_A	206_K	0.965	0.98
245_S	248_G	0.964	0.98
27_A	272_V	0.959	0.98
11_G	127_D	0.951	0.98
145_T	148_D	0.944	0.98
75_E	84_K	0.944	0.98
107_M	111_K	0.943	0.98
209_H	240_L	0.940	0.98
294_H	299_I	0.934	0.98
4_I	98_V	0.929	0.98
178_L	191_I	0.928	0.98
113_T	280_G	0.926	0.98
163_T	234_I	0.918	0.97
157_A	164_Y	0.912	0.97
143_L	146_V	0.909	0.97
5_G	97_L	0.906	0.97
45_I	88_N	0.904	0.97
153_I	164_Y	0.901	0.97
135_F	263_L	0.900	0.97
8_T	66_T	0.899	0.97
165_V	242_T	0.890	0.97
6_V	20_V	0.889	0.97
119_C	296_I	0.868	0.96
16_M	124_G	0.865	0.96
83_E	90_K	0.857	0.96
100_I	124_G	0.855	0.96
200_E	204_R	0.853	0.96
126_I	169_M	0.850	0.96
135_F	259_A	0.850	0.96
86_I	117_F	0.844	0.96
166_I	217_I	0.843	0.96
259_A	263_L	0.840	0.96
231_G	242_T	0.839	0.96
296_I	300_L	0.836	0.96
228_V	231_G	0.830	0.95
126_I	176_I	0.829	0.95
26_K	30_H	0.827	0.95
202_I	206_K	0.824	0.95
164_Y	245_S	0.822	0.95
266_R	291_L	0.818	0.95
5_G	35_Y	0.818	0.95
11_G	72_R	0.816	0.95
258_A	262_V	0.814	0.95
72_R	108_G	0.809	0.95
84_K	88_N	0.807	0.95
238_T	242_T	0.806	0.95
132_G	299_I	0.801	0.95
103_D	107_M	0.801	0.95
208_G	216_S	0.798	0.94
82_R	115_H	0.798	0.94
228_V	246_V	0.796	0.94
165_V	234_I	0.795	0.94
227_G	244_V	0.794	0.94
4_I	272_V	0.793	0.94
12_D	156_T	0.791	0.94
13_S	16_M	0.789	0.94
73_C	104_G	0.786	0.94
131_P	299_I	0.784	0.94
96_G	118_P	0.784	0.94
127_D	171_R	0.784	0.94
147_I	184_G	0.781	0.94
263_L	266_R	0.780	0.94
25_R	265_S	0.778	0.94
113_T	119_C	0.774	0.93
69_Y	214_K	0.773	0.93
4_I	96_G	0.770	0.93
132_G	294_H	0.770	0.93
188_S	204_R	0.763	0.93
118_P	280_G	0.763	0.93
86_I	112_L	0.762	0.93
203_A	210_E	0.757	0.93
146_V	247_L	0.754	0.93
181_G	186_A	0.754	0.93
88_N	92_L	0.753	0.92
231_G	238_T	0.749	0.92
20_V	100_I	0.749	0.92
143_L	258_A	0.748	0.92
20_V	67_K	0.748	0.92
267_L	286_I	0.747	0.92
81_G	84_K	0.743	0.92
24_V	52_L	0.741	0.92
73_C	81_G	0.732	0.91
234_I	242_T	0.732	0.91
174_G	192_P	0.731	0.91
165_V	231_G	0.730	0.91
267_L	291_L	0.726	0.91
199_H	203_A	0.726	0.91
76_F	81_G	0.724	0.91
198_M	202_I	0.723	0.91
262_V	266_R	0.722	0.91
122_V	284_V	0.722	0.91
284_V	293_D	0.720	0.91
8_T	12_D	0.719	0.91
130_I	299_I	0.718	0.91
195_D	225_G	0.717	0.91
19_A	100_I	0.716	0.90
58_G	62_H	0.715	0.90
107_M	300_L	0.714	0.90
66_T	159_S	0.714	0.90
130_I	267_L	0.711	0.90
227_G	234_I	0.710	0.90
274_L	279_K	0.710	0.90
103_D	173_A	0.707	0.90
292_V	295_D	0.707	0.90
168_V	176_I	0.707	0.90
104_G	127_D	0.707	0.90
286_I	289_N	0.705	0.90
158_T	214_K	0.704	0.90
140_D	259_A	0.704	0.90
277_E	293_D	0.702	0.90
188_S	218_I	0.701	0.90
14_P	145_T	0.700	0.89
26_K	29_Y	0.697	0.89
107_M	110_K	0.697	0.89
89_L	117_F	0.696	0.89
132_G	302_T	0.696	0.89
5_G	49_I	0.694	0.89
267_L	284_V	0.693	0.89
122_V	137_I	0.693	0.89
26_K	272_V	0.690	0.89
24_V	27_A	0.688	0.89
228_V	244_V	0.686	0.89
139_F	175_D	0.683	0.88
287_Q	290_K	0.681	0.88
11_G	103_D	0.681	0.88
206_K	209_H	0.679	0.88
239_N	242_T	0.675	0.88
52_L	67_K	0.670	0.87
274_L	277_E	0.669	0.87
247_L	251_Q	0.669	0.87
173_A	179_W	0.665	0.87
122_V	271_A	0.665	0.87
89_L	118_P	0.664	0.87
129_D	252_R	0.660	0.87
225_G	230_F	0.660	0.87
30_H	273_E	0.659	0.86
21_R	25_R	0.658	0.86
199_H	202_I	0.658	0.86
37_I	88_N	0.657	0.86
37_I	94_I	0.656	0.86
191_I	223_G	0.653	0.86
132_G	285_G	0.652	0.86
41_Y	44_L	0.649	0.86
72_R	127_D	0.648	0.86
52_L	57_V	0.648	0.86
44_L	97_L	0.648	0.86
103_D	171_R	0.647	0.85
169_M	251_Q	0.647	0.85
66_T	155_D	0.644	0.85
273_E	276_L	0.640	0.85
143_L	179_W	0.638	0.85
192_P	223_G	0.636	0.85
56_S	59_D	0.635	0.84
66_T	70_T	0.632	0.84
4_I	275_L	0.630	0.84
266_R	286_I	0.628	0.84
32_V	275_L	0.624	0.83
9_S	75_E	0.624	0.83
204_R	208_G	0.624	0.83
90_K	116_G	0.624	0.83
6_V	27_A	0.623	0.83
243_R	249_H	0.622	0.83
90_K	93_G	0.622	0.83
166_I	219_I	0.622	0.83
61_I	265_S	0.620	0.83
246_V	251_Q	0.620	0.83
13_S	124_G	0.619	0.83
163_T	218_I	0.619	0.83
42_A	75_E	0.618	0.83
175_D	307_E	0.617	0.83
177_A	189_I	0.617	0.83
127_D	169_M	0.614	0.82
163_T	216_S	0.613	0.82
9_S	108_G	0.611	0.82
206_K	238_T	0.611	0.82
154_R	215_H	0.608	0.82
163_T	215_H	0.607	0.82
82_R	108_G	0.607	0.82
259_A	266_R	0.607	0.82
219_I	230_F	0.607	0.82
270_Y	277_E	0.606	0.82
165_V	227_G	0.606	0.82
11_G	104_G	0.606	0.82
201_I	205_L	0.604	0.81
3_R	94_I	0.603	0.81
146_V	168_V	0.600	0.81
146_V	150_I	0.597	0.81
25_R	58_G	0.597	0.81
220_V	227_G	0.596	0.81
57_V	60_I	0.595	0.80
294_H	298_E	0.593	0.80
130_I	171_R	0.591	0.80
124_G	137_I	0.590	0.80
172_H	225_G	0.589	0.80
11_G	222_E	0.589	0.80
75_E	85_G	0.588	0.80
7_L	97_L	0.588	0.80
198_M	230_F	0.587	0.80
153_I	217_I	0.584	0.79
98_V	122_V	0.583	0.79
151_D	157_A	0.582	0.79
198_M	229_E	0.582	0.79
298_E	301_E	0.582	0.79
25_R	54_L	0.580	0.79
37_I	92_L	0.579	0.79
205_L	218_I	0.578	0.78
190_L	201_I	0.574	0.78
3_R	95_E	0.573	0.78
41_Y	104_G	0.572	0.78
41_Y	136_T	0.571	0.78
133_T	283_C	0.570	0.78
32_V	272_V	0.569	0.77
269_A	273_E	0.569	0.77
110_K	281_G	0.568	0.77
285_G	293_D	0.566	0.77
120_V	274_L	0.564	0.77
18_A	265_S	0.562	0.77
158_T	215_H	0.562	0.77
37_I	47_G	0.562	0.77
199_H	206_K	0.561	0.76
18_A	61_I	0.559	0.76
230_F	234_I	0.558	0.76
175_D	179_W	0.557	0.76
134_D	288_N	0.557	0.76
49_I	94_I	0.556	0.76
298_E	302_T	0.555	0.76
75_E	81_G	0.553	0.75
259_A	286_I	0.553	0.75
28_I	57_V	0.552	0.75
153_I	157_A	0.550	0.75
183_A	262_V	0.550	0.75
133_T	285_G	0.550	0.75
153_I	245_S	0.550	0.75
14_P	141_T	0.549	0.75
154_R	217_I	0.549	0.75
74_P	85_G	0.548	0.75
79_V	115_H	0.548	0.75
283_C	296_I	0.547	0.75
129_D	171_R	0.547	0.75
77_K	81_G	0.547	0.75
82_R	86_I	0.547	0.75
15_G	19_A	0.546	0.74
29_Y	276_L	0.545	0.74
9_S	12_D	0.544	0.74
47_G	84_K	0.543	0.74
103_D	172_H	0.543	0.74
22_A	137_I	0.542	0.74
191_I	194_A	0.540	0.74
171_R	223_G	0.539	0.74
143_L	180_A	0.538	0.73
76_F	82_R	0.537	0.73
195_D	224_V	0.537	0.73
58_G	208_G	0.537	0.73
25_R	56_S	0.537	0.73
10_G	102_G	0.532	0.73
63_R	255_S	0.532	0.73
110_K	296_I	0.530	0.72
68_L	100_I	0.530	0.72
59_D	215_H	0.529	0.72
85_G	112_L	0.529	0.72
103_D	127_D	0.529	0.72
46_S	75_E	0.528	0.72
11_G	129_D	0.527	0.72
196_Y	224_V	0.526	0.72
129_D	169_M	0.524	0.71
145_T	254_G	0.519	0.71
25_R	29_Y	0.518	0.71
202_I	233_R	0.515	0.70
190_L	196_Y	0.515	0.70
190_L	204_R	0.514	0.70
41_Y	73_C	0.514	0.70
134_D	287_Q	0.514	0.70
275_L	278_G	0.513	0.70
144_N	258_A	0.512	0.70
276_L	279_K	0.512	0.70
188_S	216_S	0.511	0.70
40_G	72_R	0.511	0.70
235_E	238_T	0.510	0.69
267_L	285_G	0.510	0.69
71_A	109_A	0.510	0.69
103_D	129_D	0.510	0.69
6_V	68_L	0.509	0.69
302_T	305_T	0.508	0.69
297_I	301_E	0.506	0.69
297_I	300_L	0.506	0.69
44_L	85_G	0.506	0.69
5_G	89_L	0.505	0.69
104_G	107_M	0.503	0.68
15_G	20_V	0.503	0.68
208_G	215_H	0.502	0.68
148_D	151_D	0.501	0.68
80_E	83_E	0.501	0.68
110_K	300_L	0.500	0.68

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1zxxA	3	1	100	0.026	Contact Map
4i7eA	3	1	100	0.026	Contact Map
1pfkA	3	0.9969	100	0.038	Contact Map
3o8oB	1	1	100	0.048	Contact Map
3opyB	2	0.9969	100	0.051	Contact Map
4xz2A	3	0.9843	100	0.051	Contact Map
3o8oA	1	0.9969	100	0.057	Contact Map
3opyA	2	0.9969	100	0.06	Contact Map
3hnoA	2	0.9875	100	0.087	Contact Map
2higA	3	0.9812	100	0.098	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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