GREMLIN Database
PFKA - ATP-dependent 6-phosphofructokinase
UniProt: O34529 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 319 (304)
Sequences: 4644 (2510)
Seq/√Len: 143.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
3_R33_E4.5131.00
33_E51_K3.7511.00
181_G189_I3.3811.00
89_L94_I3.2341.00
5_G37_I3.2141.00
38_Y50_E3.2111.00
99_V105_S3.0491.00
282_R293_D2.9591.00
87_A91_K2.8781.00
110_K114_E2.8351.00
140_D257_S2.6411.00
53_E56_S2.6131.00
47_G91_K2.5581.00
177_A219_I2.4841.00
36_G68_L2.4311.00
47_G92_L2.4261.00
274_L293_D2.3921.00
146_V176_I2.3571.00
279_K282_R2.3181.00
233_R237_E2.2141.00
232_K236_E2.1851.00
38_Y48_K2.1821.00
42_A46_S2.1801.00
199_H237_E2.1771.00
47_G88_N2.1751.00
35_Y51_K2.1531.00
49_I92_L2.1531.00
150_I219_I2.1511.00
88_N91_K2.1481.00
140_D144_N2.0571.00
78_T81_G2.0541.00
32_V276_L2.0521.00
206_K210_E2.0501.00
35_Y49_I2.0351.00
143_L176_I2.0181.00
27_A34_V1.9971.00
6_V23_V1.9881.00
36_G52_L1.9181.00
5_G94_I1.9081.00
3_R35_Y1.9011.00
270_Y273_E1.8771.00
144_N257_S1.8641.00
23_V98_V1.8621.00
26_K273_E1.8381.00
191_I306_V1.8321.00
285_G292_V1.8171.00
233_R236_E1.8001.00
83_E87_A1.7991.00
139_F179_W1.7661.00
168_V219_I1.7441.00
39_N48_K1.7361.00
234_I238_T1.7301.00
37_I49_I1.7151.00
270_Y291_L1.7061.00
279_K293_D1.6941.00
282_R295_D1.6801.00
146_V180_A1.6741.00
287_Q292_V1.6641.00
28_I54_L1.6561.00
3_R93_G1.6531.00
114_E297_I1.6531.00
273_E277_E1.6451.00
60_I67_K1.6311.00
149_A250_I1.6201.00
202_I237_E1.6021.00
30_H276_L1.5821.00
207_R210_E1.5721.00
98_V275_L1.5641.00
153_I166_I1.5641.00
37_I44_L1.5541.00
50_E56_S1.5531.00
39_N161_E1.5431.00
148_D152_K1.5321.00
19_A122_V1.5251.00
188_S201_I1.5241.00
106_Y121_G1.4931.00
39_N69_Y1.4801.00
111_K114_E1.4781.00
87_A90_K1.4731.00
150_I166_I1.4681.00
165_V230_F1.4461.00
86_I115_H1.4401.00
74_P77_K1.4141.00
202_I234_I1.4071.00
228_V232_K1.3741.00
198_M233_R1.3671.00
41_Y108_G1.3511.00
205_L216_S1.3291.00
150_I184_G1.3261.00
96_G275_L1.3151.00
137_I264_A1.3141.00
84_K87_A1.2971.00
23_V272_V1.2891.00
295_D298_E1.2751.00
231_G244_V1.2701.00
21_R265_S1.2601.00
203_A207_R1.2591.00
287_Q294_H1.2461.00
220_V230_F1.2461.00
151_D261_R1.2351.00
285_G294_H1.2351.00
274_L282_R1.2311.00
270_Y274_L1.2251.00
35_Y92_L1.2241.00
165_V218_I1.2231.00
86_I90_K1.2231.00
168_V177_A1.2221.00
113_T281_G1.2181.00
150_I217_I1.2161.00
27_A32_V1.2061.00
7_L44_L1.1981.00
9_S105_S1.1951.00
79_V83_E1.1871.00
206_K237_E1.1741.00
229_E233_R1.1631.00
19_A137_I1.1581.00
270_Y293_D1.1460.99
110_K297_I1.1430.99
45_I73_C1.1390.99
23_V268_G1.1330.99
24_V57_V1.1290.99
6_V100_I1.1280.99
245_S250_I1.1200.99
187_E213_K1.1130.99
197_D200_E1.1120.99
200_E203_A1.1090.99
120_V282_R1.1040.99
283_C294_H1.0930.99
8_T16_M1.0910.99
109_A119_C1.0890.99
99_V109_A1.0760.99
142_A176_I1.0720.99
7_L37_I1.0710.99
187_E208_G1.0690.99
274_L284_V1.0680.99
89_L97_L1.0660.99
104_G108_G1.0630.99
26_K269_A1.0600.99
150_I180_A1.0580.99
106_Y283_C1.0570.99
131_P302_T1.0570.99
109_A296_I1.0490.99
235_E241_E1.0390.99
74_P104_G1.0370.99
204_R207_R1.0370.99
174_G193_E1.0310.99
83_E115_H1.0280.99
145_T152_K1.0260.99
82_R111_K1.0190.99
69_Y161_E1.0180.99
4_I27_A1.0130.99
229_E232_K1.0110.99
207_R211_R1.0070.99
24_V28_I1.0060.99
24_V34_V0.9940.99
232_K235_E0.9940.99
7_L99_V0.9910.98
35_Y94_I0.9840.98
231_G235_E0.9820.98
22_A268_G0.9740.98
208_G211_R0.9730.98
42_A73_C0.9710.98
172_H303_K0.9700.98
203_A206_K0.9650.98
245_S248_G0.9640.98
27_A272_V0.9590.98
11_G127_D0.9510.98
145_T148_D0.9440.98
75_E84_K0.9440.98
107_M111_K0.9430.98
209_H240_L0.9400.98
294_H299_I0.9340.98
4_I98_V0.9290.98
178_L191_I0.9280.98
113_T280_G0.9260.98
163_T234_I0.9180.97
157_A164_Y0.9120.97
143_L146_V0.9090.97
5_G97_L0.9060.97
45_I88_N0.9040.97
153_I164_Y0.9010.97
135_F263_L0.9000.97
8_T66_T0.8990.97
165_V242_T0.8900.97
6_V20_V0.8890.97
119_C296_I0.8680.96
16_M124_G0.8650.96
83_E90_K0.8570.96
100_I124_G0.8550.96
200_E204_R0.8530.96
126_I169_M0.8500.96
135_F259_A0.8500.96
86_I117_F0.8440.96
166_I217_I0.8430.96
259_A263_L0.8400.96
231_G242_T0.8390.96
296_I300_L0.8360.96
228_V231_G0.8300.95
126_I176_I0.8290.95
26_K30_H0.8270.95
202_I206_K0.8240.95
164_Y245_S0.8220.95
266_R291_L0.8180.95
5_G35_Y0.8180.95
11_G72_R0.8160.95
258_A262_V0.8140.95
72_R108_G0.8090.95
84_K88_N0.8070.95
238_T242_T0.8060.95
132_G299_I0.8010.95
103_D107_M0.8010.95
208_G216_S0.7980.94
82_R115_H0.7980.94
228_V246_V0.7960.94
165_V234_I0.7950.94
227_G244_V0.7940.94
4_I272_V0.7930.94
12_D156_T0.7910.94
13_S16_M0.7890.94
73_C104_G0.7860.94
131_P299_I0.7840.94
96_G118_P0.7840.94
127_D171_R0.7840.94
147_I184_G0.7810.94
263_L266_R0.7800.94
25_R265_S0.7780.94
113_T119_C0.7740.93
69_Y214_K0.7730.93
4_I96_G0.7700.93
132_G294_H0.7700.93
188_S204_R0.7630.93
118_P280_G0.7630.93
86_I112_L0.7620.93
203_A210_E0.7570.93
146_V247_L0.7540.93
181_G186_A0.7540.93
88_N92_L0.7530.92
231_G238_T0.7490.92
20_V100_I0.7490.92
143_L258_A0.7480.92
20_V67_K0.7480.92
267_L286_I0.7470.92
81_G84_K0.7430.92
24_V52_L0.7410.92
73_C81_G0.7320.91
234_I242_T0.7320.91
174_G192_P0.7310.91
165_V231_G0.7300.91
267_L291_L0.7260.91
199_H203_A0.7260.91
76_F81_G0.7240.91
198_M202_I0.7230.91
262_V266_R0.7220.91
122_V284_V0.7220.91
284_V293_D0.7200.91
8_T12_D0.7190.91
130_I299_I0.7180.91
195_D225_G0.7170.91
19_A100_I0.7160.90
58_G62_H0.7150.90
107_M300_L0.7140.90
66_T159_S0.7140.90
130_I267_L0.7110.90
227_G234_I0.7100.90
274_L279_K0.7100.90
103_D173_A0.7070.90
292_V295_D0.7070.90
168_V176_I0.7070.90
104_G127_D0.7070.90
286_I289_N0.7050.90
158_T214_K0.7040.90
140_D259_A0.7040.90
277_E293_D0.7020.90
188_S218_I0.7010.90
14_P145_T0.7000.89
26_K29_Y0.6970.89
107_M110_K0.6970.89
89_L117_F0.6960.89
132_G302_T0.6960.89
5_G49_I0.6940.89
267_L284_V0.6930.89
122_V137_I0.6930.89
26_K272_V0.6900.89
24_V27_A0.6880.89
228_V244_V0.6860.89
139_F175_D0.6830.88
287_Q290_K0.6810.88
11_G103_D0.6810.88
206_K209_H0.6790.88
239_N242_T0.6750.88
52_L67_K0.6700.87
274_L277_E0.6690.87
247_L251_Q0.6690.87
173_A179_W0.6650.87
122_V271_A0.6650.87
89_L118_P0.6640.87
129_D252_R0.6600.87
225_G230_F0.6600.87
30_H273_E0.6590.86
21_R25_R0.6580.86
199_H202_I0.6580.86
37_I88_N0.6570.86
37_I94_I0.6560.86
191_I223_G0.6530.86
132_G285_G0.6520.86
41_Y44_L0.6490.86
72_R127_D0.6480.86
52_L57_V0.6480.86
44_L97_L0.6480.86
103_D171_R0.6470.85
169_M251_Q0.6470.85
66_T155_D0.6440.85
273_E276_L0.6400.85
143_L179_W0.6380.85
192_P223_G0.6360.85
56_S59_D0.6350.84
66_T70_T0.6320.84
4_I275_L0.6300.84
266_R286_I0.6280.84
32_V275_L0.6240.83
9_S75_E0.6240.83
204_R208_G0.6240.83
90_K116_G0.6240.83
6_V27_A0.6230.83
243_R249_H0.6220.83
90_K93_G0.6220.83
166_I219_I0.6220.83
61_I265_S0.6200.83
246_V251_Q0.6200.83
13_S124_G0.6190.83
163_T218_I0.6190.83
42_A75_E0.6180.83
175_D307_E0.6170.83
177_A189_I0.6170.83
127_D169_M0.6140.82
163_T216_S0.6130.82
9_S108_G0.6110.82
206_K238_T0.6110.82
154_R215_H0.6080.82
163_T215_H0.6070.82
82_R108_G0.6070.82
259_A266_R0.6070.82
219_I230_F0.6070.82
270_Y277_E0.6060.82
165_V227_G0.6060.82
11_G104_G0.6060.82
201_I205_L0.6040.81
3_R94_I0.6030.81
146_V168_V0.6000.81
146_V150_I0.5970.81
25_R58_G0.5970.81
220_V227_G0.5960.81
57_V60_I0.5950.80
294_H298_E0.5930.80
130_I171_R0.5910.80
124_G137_I0.5900.80
172_H225_G0.5890.80
11_G222_E0.5890.80
75_E85_G0.5880.80
7_L97_L0.5880.80
198_M230_F0.5870.80
153_I217_I0.5840.79
98_V122_V0.5830.79
151_D157_A0.5820.79
198_M229_E0.5820.79
298_E301_E0.5820.79
25_R54_L0.5800.79
37_I92_L0.5790.79
205_L218_I0.5780.78
190_L201_I0.5740.78
3_R95_E0.5730.78
41_Y104_G0.5720.78
41_Y136_T0.5710.78
133_T283_C0.5700.78
32_V272_V0.5690.77
269_A273_E0.5690.77
110_K281_G0.5680.77
285_G293_D0.5660.77
120_V274_L0.5640.77
18_A265_S0.5620.77
158_T215_H0.5620.77
37_I47_G0.5620.77
199_H206_K0.5610.76
18_A61_I0.5590.76
230_F234_I0.5580.76
175_D179_W0.5570.76
134_D288_N0.5570.76
49_I94_I0.5560.76
298_E302_T0.5550.76
75_E81_G0.5530.75
259_A286_I0.5530.75
28_I57_V0.5520.75
153_I157_A0.5500.75
183_A262_V0.5500.75
133_T285_G0.5500.75
153_I245_S0.5500.75
14_P141_T0.5490.75
154_R217_I0.5490.75
74_P85_G0.5480.75
79_V115_H0.5480.75
283_C296_I0.5470.75
129_D171_R0.5470.75
77_K81_G0.5470.75
82_R86_I0.5470.75
15_G19_A0.5460.74
29_Y276_L0.5450.74
9_S12_D0.5440.74
47_G84_K0.5430.74
103_D172_H0.5430.74
22_A137_I0.5420.74
191_I194_A0.5400.74
171_R223_G0.5390.74
143_L180_A0.5380.73
76_F82_R0.5370.73
195_D224_V0.5370.73
58_G208_G0.5370.73
25_R56_S0.5370.73
10_G102_G0.5320.73
63_R255_S0.5320.73
110_K296_I0.5300.72
68_L100_I0.5300.72
59_D215_H0.5290.72
85_G112_L0.5290.72
103_D127_D0.5290.72
46_S75_E0.5280.72
11_G129_D0.5270.72
196_Y224_V0.5260.72
129_D169_M0.5240.71
145_T254_G0.5190.71
25_R29_Y0.5180.71
202_I233_R0.5150.70
190_L196_Y0.5150.70
190_L204_R0.5140.70
41_Y73_C0.5140.70
134_D287_Q0.5140.70
275_L278_G0.5130.70
144_N258_A0.5120.70
276_L279_K0.5120.70
188_S216_S0.5110.70
40_G72_R0.5110.70
235_E238_T0.5100.69
267_L285_G0.5100.69
71_A109_A0.5100.69
103_D129_D0.5100.69
6_V68_L0.5090.69
302_T305_T0.5080.69
297_I301_E0.5060.69
297_I300_L0.5060.69
44_L85_G0.5060.69
5_G89_L0.5050.69
104_G107_M0.5030.68
15_G20_V0.5030.68
208_G215_H0.5020.68
148_D151_D0.5010.68
80_E83_E0.5010.68
110_K300_L0.5000.68
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zxxA 3 1 100 0.026 Contact Map
4i7eA 3 1 100 0.026 Contact Map
1pfkA 3 0.9969 100 0.038 Contact Map
3o8oB 1 1 100 0.048 Contact Map
3opyB 2 0.9969 100 0.051 Contact Map
4xz2A 3 0.9843 100 0.051 Contact Map
3o8oA 1 0.9969 100 0.057 Contact Map
3opyA 2 0.9969 100 0.06 Contact Map
3hnoA 2 0.9875 100 0.087 Contact Map
2higA 3 0.9812 100 0.098 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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