GREMLIN Database
SPPA - Putative signal peptide peptidase SppA
UniProt: O34525 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 335 (256)
Sequences: 2008 (1405)
Seq/√Len: 87.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
105_I129_I4.7571.00
172_V223_S4.4821.00
109_V153_S4.1831.00
158_K264_E3.7361.00
138_Y278_D3.6691.00
237_M242_V3.5831.00
139_V156_A3.1351.00
161_A262_V3.0811.00
160_F274_A3.0561.00
94_E125_K2.9551.00
241_E244_K2.9081.00
158_K274_A2.8731.00
104_G275_M2.6911.00
206_R212_E2.5271.00
197_A211_E2.3861.00
262_V265_L2.3851.00
163_P265_L2.3811.00
130_K157_D2.2931.00
93_L126_L2.2771.00
257_K262_V2.2101.00
275_M279_H2.1861.00
93_L129_I2.1651.00
238_P241_E2.0741.00
245_I259_L2.0471.00
225_E239_K2.0321.00
194_K200_D2.0071.00
231_I242_V1.9851.00
228_V239_K1.9071.00
126_L137_I1.8831.00
127_E131_K1.8811.00
66_S110_N1.8531.00
64_E108_K1.8521.00
65_V84_Y1.8431.00
255_Q258_K1.7951.00
86_H90_L1.7871.00
63_L96_A1.7841.00
254_R257_K1.7761.00
217_Q221_D1.7731.00
123_H151_Y1.7671.00
229_D233_K1.7631.00
59_K288_I1.7261.00
106_V140_S1.7251.00
115_G118_E1.7201.00
125_K128_E1.6611.00
71_D86_H1.6381.00
229_D239_K1.6301.00
274_A278_D1.6181.00
65_V152_I1.6051.00
252_D255_Q1.6031.00
68_T112_P1.6001.00
106_V268_Y1.5961.00
228_V243_K1.5951.00
141_M167_T1.5841.00
268_Y272_I1.5391.00
257_K264_E1.5081.00
276_K285_A1.5051.00
107_L126_L1.4951.00
122_I152_I1.4810.99
267_F270_D1.4790.99
154_T234_G1.4790.99
226_G230_V1.4730.99
198_H212_E1.4690.99
97_K125_K1.4600.99
72_N85_N1.4380.99
264_E274_A1.4300.99
245_I251_Y1.4290.99
110_N143_S1.4280.99
239_K243_K1.4130.99
243_K247_D1.4100.99
254_R258_K1.3930.99
61_A102_V1.3870.99
210_K213_K1.3870.99
97_K129_I1.3690.99
108_K140_S1.3660.99
63_L92_N1.3630.99
274_A277_K1.3630.99
181_K184_D1.3490.99
100_K103_K1.3450.99
97_K128_E1.3420.99
124_K128_E1.3300.99
181_K185_K1.3250.99
200_D206_R1.3160.99
179_Y183_A1.3070.99
160_F271_T1.3020.99
60_I106_V1.2980.99
141_M165_T1.2950.99
193_I202_M1.2910.99
106_V279_H1.2860.99
257_K265_L1.2820.99
107_L139_V1.2710.98
65_V89_F1.2630.98
138_Y275_M1.2410.98
59_K99_D1.2310.98
102_V135_K1.2160.98
93_L105_I1.2090.98
96_A105_I1.1890.98
58_S103_K1.1850.98
179_Y205_S1.1820.97
96_A135_K1.1820.97
266_G271_T1.1800.97
225_E243_K1.1680.97
144_M166_L1.1680.97
160_F270_D1.1670.97
119_S148_G1.1550.97
84_Y89_F1.1390.97
127_E233_K1.1340.97
271_T275_M1.1330.97
138_Y271_T1.1210.97
160_F264_E1.1180.96
106_V275_M1.1040.96
208_M213_K1.1020.96
170_L231_I1.0970.96
172_V220_V1.0760.96
232_S237_M1.0430.95
145_A149_G1.0280.94
220_V223_S1.0200.94
62_V268_Y1.0100.94
127_E130_K1.0080.94
193_I217_Q1.0050.94
89_F107_L1.0040.94
255_Q259_L0.9970.93
253_G266_G0.9940.93
154_T231_I0.9910.93
104_G279_H0.9860.93
63_L93_L0.9800.93
270_D273_T0.9770.93
210_K214_N0.9710.92
59_K102_V0.9670.92
120_A230_V0.9580.92
120_A151_Y0.9530.92
70_Q118_E0.9500.92
120_A226_G0.9440.91
112_P149_G0.9420.91
158_K278_D0.9390.91
59_K286_S0.9310.91
147_S169_S0.9290.91
183_A190_F0.9260.91
127_E234_G0.9250.91
69_I115_G0.9190.90
74_D88_T0.9180.90
183_A205_S0.9160.90
63_L89_F0.9160.90
141_M145_A0.9140.90
104_G138_Y0.9020.89
127_E155_A0.9020.89
94_E98_D0.8960.89
99_D135_K0.8950.89
182_L186_L0.8890.89
85_N88_T0.8880.89
105_I137_I0.8790.88
129_I134_K0.8700.88
162_T267_F0.8650.87
96_A102_V0.8560.87
282_L285_A0.8430.86
237_M241_E0.8430.86
90_L93_L0.8410.86
74_D85_N0.8340.85
190_F205_S0.8270.85
114_G169_S0.8260.85
123_H230_V0.8210.84
238_P245_I0.8200.84
157_D278_D0.8160.84
154_T228_V0.8140.84
179_Y204_P0.8110.84
243_K246_A0.8110.84
199_K207_E0.8110.84
147_S227_F0.8100.84
114_G147_S0.8090.84
123_H154_T0.8070.83
113_G169_S0.8070.83
113_G147_S0.8070.83
123_H127_E0.8060.83
134_K277_K0.8020.83
228_V232_S0.8010.83
160_F265_L0.7980.83
151_Y230_V0.7940.82
68_T72_N0.7910.82
57_S284_N0.7870.82
138_Y279_H0.7830.82
147_S168_G0.7830.82
64_E289_S0.7790.81
192_T200_D0.7780.81
209_T212_E0.7770.81
149_G251_Y0.7770.81
107_L152_I0.7740.81
114_G168_G0.7730.81
170_L248_G0.7730.81
169_S227_F0.7700.80
123_H226_G0.7650.80
308_F311_E0.7640.80
129_I133_T0.7620.80
266_G270_D0.7550.79
238_P243_K0.7540.79
181_K189_S0.7540.79
264_E270_D0.7500.79
108_K142_G0.7460.78
72_N115_G0.7440.78
69_I72_N0.7390.78
90_L121_E0.7320.77
109_V139_V0.7310.77
161_A257_K0.7280.77
113_G227_F0.7260.76
134_K157_D0.7260.76
121_E125_K0.7250.76
182_L185_K0.7250.76
200_D212_E0.7160.75
111_S119_S0.7150.75
60_I104_G0.7150.75
138_Y274_A0.7130.75
304_A307_M0.7110.75
60_I140_S0.7080.75
235_R263_D0.7060.74
135_K157_D0.6990.74
60_I279_H0.6970.73
86_H118_E0.6970.73
139_V153_S0.6960.73
114_G227_F0.6950.73
111_S149_G0.6920.73
211_E215_I0.6910.73
194_K206_R0.6910.73
104_G271_T0.6860.72
297_G303_G0.6850.72
173_I195_S0.6840.72
168_G227_F0.6830.72
180_S184_D0.6830.72
50_T54_G0.6810.72
113_G168_G0.6810.72
155_A234_G0.6790.72
253_G262_V0.6790.72
130_K156_A0.6710.71
136_P232_S0.6700.71
303_G308_F0.6650.70
170_L227_F0.6640.70
86_H121_E0.6630.70
92_N96_A0.6630.70
117_Y121_E0.6620.70
138_Y158_K0.6610.69
301_S304_A0.6580.69
89_F152_I0.6560.69
130_K155_A0.6550.69
105_I282_L0.6530.69
60_I287_V0.6510.68
176_S208_M0.6500.68
181_K296_L0.6480.68
90_L125_K0.6460.68
299_L304_A0.6460.68
116_V223_S0.6460.68
303_G306_K0.6450.68
183_A188_I0.6440.68
304_A308_F0.6430.67
174_M216_M0.6390.67
60_I275_M0.6390.67
157_D264_E0.6380.67
123_H234_G0.6370.67
96_A129_I0.6360.67
296_L299_L0.6340.66
216_M281_D0.6310.66
59_K101_T0.6310.66
225_E228_V0.6300.66
147_S170_L0.6280.66
199_K247_D0.6270.65
221_D225_E0.6230.65
89_F93_L0.6200.65
272_I276_K0.6200.65
105_I135_K0.6200.65
111_S248_G0.6190.64
167_T250_V0.6170.64
206_R209_T0.6150.64
272_I287_V0.6120.64
228_V247_D0.6120.64
247_D251_Y0.6110.63
140_S165_T0.6100.63
302_M307_M0.6090.63
117_Y189_S0.6080.63
172_V227_F0.6070.63
62_V272_I0.6070.63
125_K129_I0.6050.63
198_H211_E0.6050.63
271_T278_D0.6030.62
196_G199_K0.6020.62
240_A243_K0.6020.62
146_A169_S0.6010.62
271_T279_H0.6000.62
173_I192_T0.6000.62
112_P118_E0.5970.62
298_S301_S0.5970.62
131_K185_K0.5960.62
307_M310_S0.5950.61
84_Y152_I0.5950.61
154_T185_K0.5930.61
198_H206_R0.5930.61
298_S305_N0.5900.61
242_V246_A0.5870.60
214_N218_S0.5860.60
62_V289_S0.5850.60
154_T242_V0.5840.60
169_S172_V0.5840.60
146_A166_L0.5840.60
296_L304_A0.5830.60
193_I211_E0.5830.60
151_Y155_A0.5820.60
123_H155_A0.5820.60
141_M161_A0.5810.60
292_E295_G0.5790.59
150_Y248_G0.5780.59
219_M250_V0.5780.59
119_S122_I0.5770.59
166_L250_V0.5770.59
57_S286_S0.5730.59
306_K309_K0.5720.58
84_Y116_V0.5720.58
94_E97_K0.5680.58
59_K272_I0.5680.58
298_S302_M0.5680.58
230_V234_G0.5660.58
196_G212_E0.5630.57
218_S221_D0.5630.57
122_I142_G0.5620.57
107_L271_T0.5610.57
169_S248_G0.5610.57
194_K212_E0.5610.57
233_K254_R0.5600.57
284_N302_M0.5590.57
215_I218_S0.5580.57
161_A165_T0.5570.56
297_G301_S0.5570.56
114_G170_L0.5550.56
60_I160_F0.5550.56
63_L126_L0.5540.56
174_M212_E0.5540.56
176_S204_P0.5540.56
158_K185_K0.5530.56
241_E245_I0.5520.56
302_M306_K0.5500.55
211_E214_N0.5490.55
145_A148_G0.5480.55
69_I148_G0.5450.55
204_P302_M0.5450.55
147_S248_G0.5440.55
54_G225_E0.5440.55
104_G136_P0.5420.54
167_T262_V0.5410.54
232_S239_K0.5400.54
229_D232_S0.5400.54
225_E229_D0.5390.54
111_S114_G0.5380.54
114_G248_G0.5380.54
184_D189_S0.5350.53
224_Y232_S0.5350.53
195_S220_V0.5340.53
70_Q83_G0.5330.53
210_K258_K0.5310.53
115_G119_S0.5310.53
286_S303_G0.5300.53
168_G248_G0.5290.53
149_G169_S0.5290.53
109_V145_A0.5260.52
96_A288_I0.5260.52
121_E162_T0.5260.52
179_Y186_L0.5260.52
186_L234_G0.5240.52
57_S300_F0.5200.51
223_S226_G0.5200.51
117_Y223_S0.5190.51
260_N266_G0.5180.51
146_A168_G0.5180.51
294_F297_G0.5150.51
237_M260_N0.5150.51
224_Y249_R0.5140.51
148_G169_S0.5120.50
111_S147_S0.5120.50
73_G91_K0.5110.50
194_K201_I0.5100.50
126_L156_A0.5090.50
178_N191_E0.5090.50
146_A227_F0.5060.50
71_D87_R0.5050.49
49_K53_N0.5040.49
160_F250_V0.5030.49
173_I200_D0.5020.49
293_S302_M0.5010.49
232_S242_V0.5010.49
126_L155_A0.5010.49
203_S255_Q0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3bf0A 3 0.8418 100 0.414 Contact Map
4kwbA 5 0.7224 100 0.478 Contact Map
3rstA 3 0.6806 100 0.52 Contact Map
4gm2A 3 0.5075 100 0.69 Contact Map
3vivA 2 0.606 100 0.694 Contact Map
2f6iA 5 0.5343 100 0.706 Contact Map
3p2lA 4 0.5612 100 0.71 Contact Map
2cbyA 3 0.4985 100 0.71 Contact Map
4u0gA 4 0.5821 99.9 0.715 Contact Map
1yg6A 4 0.5672 99.9 0.718 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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