SPPA - BACSU - GREMLIN Contacts

Home
GREMLIN
- Submit - Monomer
- Submit - Complex
Contacts
Metagenomics
- Contacts
- Structures
Structures
- List
Old Projects
- Complexes (2014)
- PFAM (2013)

SPPA - Putative signal peptide peptidase SppA

UniProt:	O34525	Sequences: MNAKRWIALVIALGIFGVSIIVSISMSFFESVKGAQTDLTSLTDESQEKTLENGSPSSKIAVLEVSGTIQDNGDSSSLLGADGYNHRTFLKNLERAKDDKTVKGIVLKVNSPGGGVYESAEIHKKLEEIKKETKKPIYVSMGSMAASGGYYISTAADKIFATPETLTGSLGVIMESVNYSKLADKLGISFETIKSGAHKDIMSPSREMTKEEKNIMQSMVDNSYEGFVDVISKGRGMPKAEVKKIADGRVYDGRQAKKLNLVDELGFYDDTITAMKKDHKDLKNASVISYEESFGLGSLFSMGANKMFKSEIDFLNMREILSQSGSPRMMYLYAK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	335 (256)
Sequences:	2008 (1405)
Seq/√Len:	87.8

ECOLI - SPPA SOHB
BACSU - SPPA
HALSA - Q9HRN3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
105_I	129_I	4.757	1.00
172_V	223_S	4.482	1.00
109_V	153_S	4.183	1.00
158_K	264_E	3.736	1.00
138_Y	278_D	3.669	1.00
237_M	242_V	3.583	1.00
139_V	156_A	3.135	1.00
161_A	262_V	3.081	1.00
160_F	274_A	3.056	1.00
94_E	125_K	2.955	1.00
241_E	244_K	2.908	1.00
158_K	274_A	2.873	1.00
104_G	275_M	2.691	1.00
206_R	212_E	2.527	1.00
197_A	211_E	2.386	1.00
262_V	265_L	2.385	1.00
163_P	265_L	2.381	1.00
130_K	157_D	2.293	1.00
93_L	126_L	2.277	1.00
257_K	262_V	2.210	1.00
275_M	279_H	2.186	1.00
93_L	129_I	2.165	1.00
238_P	241_E	2.074	1.00
245_I	259_L	2.047	1.00
225_E	239_K	2.032	1.00
194_K	200_D	2.007	1.00
231_I	242_V	1.985	1.00
228_V	239_K	1.907	1.00
126_L	137_I	1.883	1.00
127_E	131_K	1.881	1.00
66_S	110_N	1.853	1.00
64_E	108_K	1.852	1.00
65_V	84_Y	1.843	1.00
255_Q	258_K	1.795	1.00
86_H	90_L	1.787	1.00
63_L	96_A	1.784	1.00
254_R	257_K	1.776	1.00
217_Q	221_D	1.773	1.00
123_H	151_Y	1.767	1.00
229_D	233_K	1.763	1.00
59_K	288_I	1.726	1.00
106_V	140_S	1.725	1.00
115_G	118_E	1.720	1.00
125_K	128_E	1.661	1.00
71_D	86_H	1.638	1.00
229_D	239_K	1.630	1.00
274_A	278_D	1.618	1.00
65_V	152_I	1.605	1.00
252_D	255_Q	1.603	1.00
68_T	112_P	1.600	1.00
106_V	268_Y	1.596	1.00
228_V	243_K	1.595	1.00
141_M	167_T	1.584	1.00
268_Y	272_I	1.539	1.00
257_K	264_E	1.508	1.00
276_K	285_A	1.505	1.00
107_L	126_L	1.495	1.00
122_I	152_I	1.481	0.99
267_F	270_D	1.479	0.99
154_T	234_G	1.479	0.99
226_G	230_V	1.473	0.99
198_H	212_E	1.469	0.99
97_K	125_K	1.460	0.99
72_N	85_N	1.438	0.99
264_E	274_A	1.430	0.99
245_I	251_Y	1.429	0.99
110_N	143_S	1.428	0.99
239_K	243_K	1.413	0.99
243_K	247_D	1.410	0.99
254_R	258_K	1.393	0.99
61_A	102_V	1.387	0.99
210_K	213_K	1.387	0.99
97_K	129_I	1.369	0.99
108_K	140_S	1.366	0.99
63_L	92_N	1.363	0.99
274_A	277_K	1.363	0.99
181_K	184_D	1.349	0.99
100_K	103_K	1.345	0.99
97_K	128_E	1.342	0.99
124_K	128_E	1.330	0.99
181_K	185_K	1.325	0.99
200_D	206_R	1.316	0.99
179_Y	183_A	1.307	0.99
160_F	271_T	1.302	0.99
60_I	106_V	1.298	0.99
141_M	165_T	1.295	0.99
193_I	202_M	1.291	0.99
106_V	279_H	1.286	0.99
257_K	265_L	1.282	0.99
107_L	139_V	1.271	0.98
65_V	89_F	1.263	0.98
138_Y	275_M	1.241	0.98
59_K	99_D	1.231	0.98
102_V	135_K	1.216	0.98
93_L	105_I	1.209	0.98
96_A	105_I	1.189	0.98
58_S	103_K	1.185	0.98
179_Y	205_S	1.182	0.97
96_A	135_K	1.182	0.97
266_G	271_T	1.180	0.97
225_E	243_K	1.168	0.97
144_M	166_L	1.168	0.97
160_F	270_D	1.167	0.97
119_S	148_G	1.155	0.97
84_Y	89_F	1.139	0.97
127_E	233_K	1.134	0.97
271_T	275_M	1.133	0.97
138_Y	271_T	1.121	0.97
160_F	264_E	1.118	0.96
106_V	275_M	1.104	0.96
208_M	213_K	1.102	0.96
170_L	231_I	1.097	0.96
172_V	220_V	1.076	0.96
232_S	237_M	1.043	0.95
145_A	149_G	1.028	0.94
220_V	223_S	1.020	0.94
62_V	268_Y	1.010	0.94
127_E	130_K	1.008	0.94
193_I	217_Q	1.005	0.94
89_F	107_L	1.004	0.94
255_Q	259_L	0.997	0.93
253_G	266_G	0.994	0.93
154_T	231_I	0.991	0.93
104_G	279_H	0.986	0.93
63_L	93_L	0.980	0.93
270_D	273_T	0.977	0.93
210_K	214_N	0.971	0.92
59_K	102_V	0.967	0.92
120_A	230_V	0.958	0.92
120_A	151_Y	0.953	0.92
70_Q	118_E	0.950	0.92
120_A	226_G	0.944	0.91
112_P	149_G	0.942	0.91
158_K	278_D	0.939	0.91
59_K	286_S	0.931	0.91
147_S	169_S	0.929	0.91
183_A	190_F	0.926	0.91
127_E	234_G	0.925	0.91
69_I	115_G	0.919	0.90
74_D	88_T	0.918	0.90
183_A	205_S	0.916	0.90
63_L	89_F	0.916	0.90
141_M	145_A	0.914	0.90
104_G	138_Y	0.902	0.89
127_E	155_A	0.902	0.89
94_E	98_D	0.896	0.89
99_D	135_K	0.895	0.89
182_L	186_L	0.889	0.89
85_N	88_T	0.888	0.89
105_I	137_I	0.879	0.88
129_I	134_K	0.870	0.88
162_T	267_F	0.865	0.87
96_A	102_V	0.856	0.87
282_L	285_A	0.843	0.86
237_M	241_E	0.843	0.86
90_L	93_L	0.841	0.86
74_D	85_N	0.834	0.85
190_F	205_S	0.827	0.85
114_G	169_S	0.826	0.85
123_H	230_V	0.821	0.84
238_P	245_I	0.820	0.84
157_D	278_D	0.816	0.84
154_T	228_V	0.814	0.84
179_Y	204_P	0.811	0.84
243_K	246_A	0.811	0.84
199_K	207_E	0.811	0.84
147_S	227_F	0.810	0.84
114_G	147_S	0.809	0.84
123_H	154_T	0.807	0.83
113_G	169_S	0.807	0.83
113_G	147_S	0.807	0.83
123_H	127_E	0.806	0.83
134_K	277_K	0.802	0.83
228_V	232_S	0.801	0.83
160_F	265_L	0.798	0.83
151_Y	230_V	0.794	0.82
68_T	72_N	0.791	0.82
57_S	284_N	0.787	0.82
138_Y	279_H	0.783	0.82
147_S	168_G	0.783	0.82
64_E	289_S	0.779	0.81
192_T	200_D	0.778	0.81
209_T	212_E	0.777	0.81
149_G	251_Y	0.777	0.81
107_L	152_I	0.774	0.81
114_G	168_G	0.773	0.81
170_L	248_G	0.773	0.81
169_S	227_F	0.770	0.80
123_H	226_G	0.765	0.80
308_F	311_E	0.764	0.80
129_I	133_T	0.762	0.80
266_G	270_D	0.755	0.79
238_P	243_K	0.754	0.79
181_K	189_S	0.754	0.79
264_E	270_D	0.750	0.79
108_K	142_G	0.746	0.78
72_N	115_G	0.744	0.78
69_I	72_N	0.739	0.78
90_L	121_E	0.732	0.77
109_V	139_V	0.731	0.77
161_A	257_K	0.728	0.77
113_G	227_F	0.726	0.76
134_K	157_D	0.726	0.76
121_E	125_K	0.725	0.76
182_L	185_K	0.725	0.76
200_D	212_E	0.716	0.75
111_S	119_S	0.715	0.75
60_I	104_G	0.715	0.75
138_Y	274_A	0.713	0.75
304_A	307_M	0.711	0.75
60_I	140_S	0.708	0.75
235_R	263_D	0.706	0.74
135_K	157_D	0.699	0.74
60_I	279_H	0.697	0.73
86_H	118_E	0.697	0.73
139_V	153_S	0.696	0.73
114_G	227_F	0.695	0.73
111_S	149_G	0.692	0.73
211_E	215_I	0.691	0.73
194_K	206_R	0.691	0.73
104_G	271_T	0.686	0.72
297_G	303_G	0.685	0.72
173_I	195_S	0.684	0.72
168_G	227_F	0.683	0.72
180_S	184_D	0.683	0.72
50_T	54_G	0.681	0.72
113_G	168_G	0.681	0.72
155_A	234_G	0.679	0.72
253_G	262_V	0.679	0.72
130_K	156_A	0.671	0.71
136_P	232_S	0.670	0.71
303_G	308_F	0.665	0.70
170_L	227_F	0.664	0.70
86_H	121_E	0.663	0.70
92_N	96_A	0.663	0.70
117_Y	121_E	0.662	0.70
138_Y	158_K	0.661	0.69
301_S	304_A	0.658	0.69
89_F	152_I	0.656	0.69
130_K	155_A	0.655	0.69
105_I	282_L	0.653	0.69
60_I	287_V	0.651	0.68
176_S	208_M	0.650	0.68
181_K	296_L	0.648	0.68
90_L	125_K	0.646	0.68
299_L	304_A	0.646	0.68
116_V	223_S	0.646	0.68
303_G	306_K	0.645	0.68
183_A	188_I	0.644	0.68
304_A	308_F	0.643	0.67
174_M	216_M	0.639	0.67
60_I	275_M	0.639	0.67
157_D	264_E	0.638	0.67
123_H	234_G	0.637	0.67
96_A	129_I	0.636	0.67
296_L	299_L	0.634	0.66
216_M	281_D	0.631	0.66
59_K	101_T	0.631	0.66
225_E	228_V	0.630	0.66
147_S	170_L	0.628	0.66
199_K	247_D	0.627	0.65
221_D	225_E	0.623	0.65
89_F	93_L	0.620	0.65
272_I	276_K	0.620	0.65
105_I	135_K	0.620	0.65
111_S	248_G	0.619	0.64
167_T	250_V	0.617	0.64
206_R	209_T	0.615	0.64
272_I	287_V	0.612	0.64
228_V	247_D	0.612	0.64
247_D	251_Y	0.611	0.63
140_S	165_T	0.610	0.63
302_M	307_M	0.609	0.63
117_Y	189_S	0.608	0.63
172_V	227_F	0.607	0.63
62_V	272_I	0.607	0.63
125_K	129_I	0.605	0.63
198_H	211_E	0.605	0.63
271_T	278_D	0.603	0.62
196_G	199_K	0.602	0.62
240_A	243_K	0.602	0.62
146_A	169_S	0.601	0.62
271_T	279_H	0.600	0.62
173_I	192_T	0.600	0.62
112_P	118_E	0.597	0.62
298_S	301_S	0.597	0.62
131_K	185_K	0.596	0.62
307_M	310_S	0.595	0.61
84_Y	152_I	0.595	0.61
154_T	185_K	0.593	0.61
198_H	206_R	0.593	0.61
298_S	305_N	0.590	0.61
242_V	246_A	0.587	0.60
214_N	218_S	0.586	0.60
62_V	289_S	0.585	0.60
154_T	242_V	0.584	0.60
169_S	172_V	0.584	0.60
146_A	166_L	0.584	0.60
296_L	304_A	0.583	0.60
193_I	211_E	0.583	0.60
151_Y	155_A	0.582	0.60
123_H	155_A	0.582	0.60
141_M	161_A	0.581	0.60
292_E	295_G	0.579	0.59
150_Y	248_G	0.578	0.59
219_M	250_V	0.578	0.59
119_S	122_I	0.577	0.59
166_L	250_V	0.577	0.59
57_S	286_S	0.573	0.59
306_K	309_K	0.572	0.58
84_Y	116_V	0.572	0.58
94_E	97_K	0.568	0.58
59_K	272_I	0.568	0.58
298_S	302_M	0.568	0.58
230_V	234_G	0.566	0.58
196_G	212_E	0.563	0.57
218_S	221_D	0.563	0.57
122_I	142_G	0.562	0.57
107_L	271_T	0.561	0.57
169_S	248_G	0.561	0.57
194_K	212_E	0.561	0.57
233_K	254_R	0.560	0.57
284_N	302_M	0.559	0.57
215_I	218_S	0.558	0.57
161_A	165_T	0.557	0.56
297_G	301_S	0.557	0.56
114_G	170_L	0.555	0.56
60_I	160_F	0.555	0.56
63_L	126_L	0.554	0.56
174_M	212_E	0.554	0.56
176_S	204_P	0.554	0.56
158_K	185_K	0.553	0.56
241_E	245_I	0.552	0.56
302_M	306_K	0.550	0.55
211_E	214_N	0.549	0.55
145_A	148_G	0.548	0.55
69_I	148_G	0.545	0.55
204_P	302_M	0.545	0.55
147_S	248_G	0.544	0.55
54_G	225_E	0.544	0.55
104_G	136_P	0.542	0.54
167_T	262_V	0.541	0.54
232_S	239_K	0.540	0.54
229_D	232_S	0.540	0.54
225_E	229_D	0.539	0.54
111_S	114_G	0.538	0.54
114_G	248_G	0.538	0.54
184_D	189_S	0.535	0.53
224_Y	232_S	0.535	0.53
195_S	220_V	0.534	0.53
70_Q	83_G	0.533	0.53
210_K	258_K	0.531	0.53
115_G	119_S	0.531	0.53
286_S	303_G	0.530	0.53
168_G	248_G	0.529	0.53
149_G	169_S	0.529	0.53
109_V	145_A	0.526	0.52
96_A	288_I	0.526	0.52
121_E	162_T	0.526	0.52
179_Y	186_L	0.526	0.52
186_L	234_G	0.524	0.52
57_S	300_F	0.520	0.51
223_S	226_G	0.520	0.51
117_Y	223_S	0.519	0.51
260_N	266_G	0.518	0.51
146_A	168_G	0.518	0.51
294_F	297_G	0.515	0.51
237_M	260_N	0.515	0.51
224_Y	249_R	0.514	0.51
148_G	169_S	0.512	0.50
111_S	147_S	0.512	0.50
73_G	91_K	0.511	0.50
194_K	201_I	0.510	0.50
126_L	156_A	0.509	0.50
178_N	191_E	0.509	0.50
146_A	227_F	0.506	0.50
71_D	87_R	0.505	0.49
49_K	53_N	0.504	0.49
160_F	250_V	0.503	0.49
173_I	200_D	0.502	0.49
293_S	302_M	0.501	0.49
232_S	242_V	0.501	0.49
126_L	155_A	0.501	0.49
203_S	255_Q	0.500	0.49

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3bf0A	3	0.8418	100	0.414	Contact Map
4kwbA	5	0.7224	100	0.478	Contact Map
3rstA	3	0.6806	100	0.52	Contact Map
4gm2A	3	0.5075	100	0.69	Contact Map
3vivA	2	0.606	100	0.694	Contact Map
2f6iA	5	0.5343	100	0.706	Contact Map
3p2lA	4	0.5612	100	0.71	Contact Map
2cbyA	3	0.4985	100	0.71	Contact Map
4u0gA	4	0.5821	99.9	0.715	Contact Map
1yg6A	4	0.5672	99.9	0.718	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0093 seconds.