GREMLIN Database
TRMB - tRNA (guanine-N(7)-)-methyltransferase
UniProt: O34522 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 213 (192)
Sequences: 3862 (2517)
Seq/√Len: 181.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
110_K144_G4.1891.00
58_K85_S4.1341.00
60_N65_Y2.6961.00
106_P142_V2.6801.00
112_V139_Y2.6261.00
173_L210_E2.6141.00
156_R160_E2.5941.00
82_V90_V2.5401.00
106_P141_E2.5361.00
30_K33_T2.4771.00
42_H63_I2.2771.00
114_L117_S2.2161.00
114_L149_I2.2101.00
151_F162_S2.1731.00
37_N64_N2.1701.00
143_M147_G2.1591.00
53_I67_G2.0941.00
137_K169_Y2.0681.00
133_S165_S2.0671.00
114_L131_T2.0501.00
42_H113_Y2.0381.00
47_T67_G1.9901.00
111_R148_S1.9041.00
140_E171_L1.8991.00
101_T138_K1.8911.00
55_G59_Q1.8341.00
42_H111_R1.8091.00
34_V91_K1.7931.00
161_Y165_S1.7801.00
40_P110_K1.7731.00
190_M195_E1.7341.00
156_R203_P1.7011.00
23_N26_D1.6901.00
167_S209_V1.6171.00
60_N63_I1.5951.00
101_T141_E1.5861.00
51_Q195_E1.5771.00
43_I109_V1.5741.00
31_W41_I1.5451.00
40_P64_N1.4921.00
55_G58_K1.4911.00
131_T162_S1.4861.00
156_R205_Y1.4841.00
195_E199_A1.4831.00
39_N62_D1.4821.00
39_N64_N1.4751.00
32_N38_D1.4691.00
57_A87_A1.4601.00
78_A92_L1.4551.00
72_K94_N1.4441.00
100_L138_K1.4301.00
153_T159_F1.4211.00
140_E172_L1.4041.00
68_I97_A1.3751.00
169_Y172_L1.3711.00
45_V97_A1.3671.00
66_I104_F1.3661.00
35_F64_N1.3431.00
109_V112_V1.3411.00
41_I64_N1.3231.00
125_H128_R1.3181.00
82_V92_L1.3141.00
148_S210_E1.3121.00
109_V142_V1.2621.00
54_S81_K1.2581.00
148_S208_E1.2451.00
107_G144_G1.2371.00
126_E157_G1.2361.00
52_F113_Y1.2271.00
175_Y208_E1.2241.00
157_G160_E1.2231.00
133_S168_E1.2221.00
75_I92_L1.2021.00
112_V143_M1.1941.00
150_H206_R1.1921.00
31_W104_F1.1751.00
104_F108_E1.1721.00
95_I103_V1.1641.00
99_T102_D1.1371.00
28_K105_E1.1341.00
177_S180_L1.1321.00
105_E108_E1.1321.00
183_S186_E1.1241.00
120_W125_H1.1231.00
57_A89_N1.1171.00
29_G108_E1.1091.00
111_R150_H1.0961.00
114_L130_L1.0661.00
126_E161_Y1.0581.00
58_K87_A1.0541.00
123_K126_E1.0521.00
107_G145_K1.0481.00
134_H137_K1.0331.00
137_K140_E1.0321.00
196_K200_L1.0311.00
43_I68_I1.0301.00
179_D182_N1.0170.99
35_F66_I1.0080.99
43_I112_V1.0050.99
140_E169_Y1.0030.99
37_N41_I0.9990.99
190_M198_S0.9980.99
136_L165_S0.9940.99
149_I166_F0.9900.99
198_S204_I0.9790.99
56_M60_N0.9730.99
45_V112_V0.9700.99
67_G78_A0.9610.99
197_F202_Q0.9610.99
27_Y30_K0.9390.99
138_K141_E0.9370.99
42_H56_M0.9070.99
52_F56_M0.9020.99
38_D64_N0.9010.99
49_K77_T0.8960.99
202_Q205_Y0.8900.99
130_L135_F0.8900.99
151_F158_L0.8850.99
54_S82_V0.8820.99
132_Y161_Y0.8810.98
133_S137_K0.8750.98
63_I111_R0.8740.98
47_T78_A0.8720.98
163_L176_V0.8590.98
51_Q81_K0.8590.98
52_F115_N0.8580.98
56_M113_Y0.8520.98
163_L209_V0.8470.98
42_H65_Y0.8470.98
75_I94_N0.8440.98
47_T69_E0.8360.98
82_V87_A0.8290.98
56_M189_I0.8280.98
54_S78_A0.8280.98
162_S207_A0.8260.98
51_Q192_E0.8250.98
81_K85_S0.8230.98
77_T81_K0.8210.98
68_I93_L0.8160.98
121_P126_E0.8100.97
53_I90_V0.8070.97
193_Y196_K0.8060.97
41_I66_I0.7980.97
120_W128_R0.7960.97
114_L139_Y0.7880.97
197_F200_L0.7870.97
188_N206_R0.7840.97
42_H60_N0.7820.97
135_F139_Y0.7780.97
73_S77_T0.7770.97
166_F207_A0.7750.97
77_T80_Q0.7710.97
130_L139_Y0.7680.96
136_L149_I0.7600.96
27_Y34_V0.7550.96
30_K108_E0.7520.96
55_G189_I0.7470.96
122_K125_H0.7400.96
54_S87_A0.7390.96
19_I22_S0.7390.96
80_Q84_D0.7340.95
49_K192_E0.7320.95
63_I110_K0.7290.95
54_S58_K0.7230.95
118_D122_K0.7230.95
31_W108_E0.7220.95
56_M65_Y0.7220.95
175_Y210_E0.7220.95
38_D88_Q0.7170.95
195_E198_S0.7170.95
126_E132_Y0.7160.95
41_I110_K0.7150.95
157_G161_Y0.7140.95
138_K142_V0.7130.95
159_F207_A0.7050.94
104_F142_V0.6990.94
36_G39_N0.6980.94
166_F209_V0.6960.94
160_E178_L0.6950.94
24_P27_Y0.6930.94
40_P63_I0.6920.94
80_Q83_K0.6880.94
71_F74_V0.6870.93
112_V147_G0.6840.93
134_H138_K0.6840.93
133_S169_Y0.6810.93
32_N41_I0.6780.93
194_E198_S0.6760.93
156_R202_Q0.6760.93
45_V114_L0.6750.93
43_I104_F0.6710.93
76_V80_Q0.6690.93
51_Q55_G0.6640.92
124_R128_R0.6630.92
65_Y113_Y0.6600.92
124_R127_K0.6580.92
164_K168_E0.6550.92
156_R178_L0.6510.92
116_F152_K0.6510.92
87_A90_V0.6450.91
166_F172_L0.6450.91
70_L98_D0.6400.91
121_P155_N0.6400.91
148_S174_T0.6330.90
144_G147_G0.6310.90
73_S132_Y0.6310.90
32_N110_K0.6310.90
140_E145_K0.6300.90
163_L174_T0.6290.90
47_T53_I0.6270.90
24_P102_D0.6260.90
137_K141_E0.6200.89
22_S93_L0.6170.89
82_V86_E0.6100.89
41_I109_V0.6080.89
78_A95_I0.6060.88
43_I100_L0.6060.88
71_F96_D0.6060.88
133_S161_Y0.6050.88
100_L142_V0.6000.88
47_T92_L0.6000.88
70_L94_N0.5990.88
24_P103_V0.5980.88
78_A90_V0.5930.87
167_S178_L0.5920.87
187_G208_E0.5880.87
68_I96_D0.5880.87
170_G173_L0.5870.87
53_I65_Y0.5850.87
197_F204_I0.5830.86
152_K159_F0.5790.86
25_A102_D0.5770.86
198_S202_Q0.5760.86
69_E205_Y0.5750.86
131_T139_Y0.5740.86
69_E97_A0.5740.86
47_T71_F0.5740.86
150_H185_L0.5740.86
137_K170_G0.5720.85
98_D138_K0.5720.85
93_L103_V0.5710.85
174_T210_E0.5690.85
118_D127_K0.5670.85
123_K164_K0.5650.85
79_V83_K0.5630.84
57_A60_N0.5620.84
114_L136_L0.5620.84
141_E145_K0.5620.84
116_F204_I0.5590.84
65_Y89_N0.5580.84
136_L166_F0.5580.84
149_I171_L0.5560.84
54_S85_S0.5550.84
136_L169_Y0.5520.83
32_N210_E0.5510.83
131_T166_F0.5510.83
121_P125_H0.5500.83
164_K167_S0.5490.83
118_D128_R0.5450.83
126_E165_S0.5420.82
44_E120_W0.5410.82
152_K194_E0.5360.81
162_S209_V0.5350.81
117_S158_L0.5340.81
189_I204_I0.5320.81
29_G105_E0.5290.81
44_E47_T0.5270.80
115_N192_E0.5270.80
19_I23_N0.5240.80
74_V118_D0.5230.80
21_I79_V0.5220.80
73_S76_V0.5200.80
163_L178_L0.5160.79
167_S174_T0.5150.79
53_I78_A0.5110.78
152_K188_N0.5100.78
158_L162_S0.5090.78
177_S181_H0.5090.78
163_L167_S0.5080.78
155_N197_F0.5070.78
192_E197_F0.5070.78
74_V193_Y0.5060.78
52_F192_E0.5060.78
120_W124_R0.5050.78
27_Y33_T0.5040.77
110_K146_G0.5030.77
36_G88_Q0.5030.77
74_V79_V0.5000.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2fcaA 2 0.9624 100 0.503 Contact Map
2vdvE 2 0.9108 100 0.528 Contact Map
3ckkA 1 0.8451 100 0.529 Contact Map
1yzhA 1 0.9671 100 0.552 Contact Map
3dxyA 1 0.9531 100 0.567 Contact Map
4rwzA 1 0.8545 99.6 0.748 Contact Map
3p2eA 1 0.8498 99.6 0.753 Contact Map
1o54A 4 0.8967 99.5 0.765 Contact Map
1i9gA 3 0.9108 99.5 0.767 Contact Map
3mb5A 3 0.9061 99.5 0.769 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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