GREMLIN Database
ASPG2 - L-asparaginase 2
UniProt: O34482 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 375 (313)
Sequences: 3397 (2439)
Seq/√Len: 137.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
151_L163_I4.3831.00
52_N97_N4.1221.00
280_D310_S4.0351.00
269_Y293_A3.6441.00
147_T163_I3.5231.00
101_E122_R3.3441.00
155_V161_V3.3341.00
200_V212_V3.1581.00
280_D307_G3.0511.00
54_R132_V2.8991.00
364_D367_K2.8681.00
367_K370_A2.8331.00
193_A240_F2.8151.00
106_V115_I2.6021.00
56_L103_I2.5991.00
358_A371_Y2.4761.00
207_A225_S2.4411.00
52_N99_S2.3931.00
99_S132_V2.3661.00
289_G355_L2.2941.00
123_I137_V2.2341.00
266_D287_A2.1781.00
136_V188_A2.1711.00
293_A348_P2.1401.00
57_A63_A2.1291.00
113_N273_N2.1061.00
309_D313_K2.0971.00
127_L135_I2.0881.00
321_S343_S2.0861.00
127_L159_K2.0761.00
127_L157_S2.0761.00
201_V206_I2.0751.00
283_V311_A2.0741.00
215_T224_K2.0581.00
207_A212_V2.0561.00
54_R126_L1.9361.00
320_R340_L1.9041.00
206_I232_G1.9041.00
120_A154_T1.8561.00
56_L101_E1.8131.00
82_V100_G1.8071.00
187_V206_I1.7981.00
183_N204_D1.7681.00
112_D115_I1.7681.00
56_L122_R1.7631.00
276_S280_D1.7521.00
319_V343_S1.7481.00
116_L272_Q1.7421.00
262_L288_K1.7301.00
294_G320_R1.7231.00
307_G310_S1.7211.00
144_L200_V1.7081.00
70_T74_E1.6901.00
319_V372_F1.6831.00
152_N210_R1.6821.00
67_Q73_T1.6791.00
104_V115_I1.6641.00
164_V202_L1.6591.00
365_P369_Q1.6431.00
103_I122_R1.6381.00
358_A368_I1.6301.00
278_L282_A1.6141.00
113_N356_M1.5861.00
111_I115_I1.5851.00
116_L353_M1.5841.00
280_D284_K1.5501.00
183_N201_V1.5321.00
215_T222_T1.5301.00
150_F154_T1.5101.00
276_S307_G1.5101.00
126_L131_D1.5091.00
319_V355_L1.5031.00
122_R125_H1.4961.00
358_A362_T1.4901.00
122_R126_L1.4821.00
182_Y186_K1.4801.00
267_I348_P1.4781.00
187_V201_V1.4681.00
123_I161_V1.4591.00
81_G84_S1.4541.00
264_Q275_G1.4491.00
365_P368_I1.4411.00
139_H147_T1.4191.00
310_S314_K1.4131.00
83_E87_E1.4031.00
317_T368_I1.3751.00
117_L154_T1.3631.00
216_N326_N1.3491.00
279_F307_G1.3461.00
279_F311_A1.3361.00
309_D338_K1.3281.00
217_T322_T1.3221.00
124_N128_A1.3001.00
197_G208_S1.2941.00
116_L150_F1.2891.00
264_Q288_K1.2781.00
321_S348_P1.2781.00
234_I238_I1.2661.00
57_A82_V1.2641.00
369_Q373_N1.2511.00
190_A193_A1.2281.00
50_L97_N1.2221.00
290_I311_A1.2201.00
193_A196_K1.2101.00
115_I118_K1.2101.00
264_Q277_Y1.2091.00
136_V189_G1.2061.00
57_A102_Q1.2051.00
175_A178_P1.2031.00
120_A124_N1.1971.00
106_V111_I1.1941.00
101_E126_L1.1911.00
196_K240_F1.1821.00
269_Y348_P1.1801.00
205_R221_D1.1700.99
110_N302_D1.1650.99
266_D282_A1.1610.99
268_I279_F1.1610.99
282_A287_A1.1530.99
300_L308_A1.1500.99
179_S183_N1.1430.99
279_F304_A1.1380.99
136_V185_V1.1270.99
131_D159_K1.1080.99
92_M185_V1.1080.99
51_P191_P1.1040.99
283_V316_V1.1030.99
191_P196_K1.1030.99
211_Y242_N1.0930.99
312_V318_V1.0920.99
52_N133_D1.0890.99
80_V170_S1.0780.99
308_A340_L1.0780.99
121_K125_H1.0770.99
233_T241_N1.0620.99
82_V85_L1.0610.99
199_L231_V1.0580.99
265_V359_L1.0560.99
123_I135_I1.0550.99
119_L137_V1.0550.99
113_N116_L1.0500.99
263_P360_T1.0500.99
281_A285_A1.0480.99
144_L165_G1.0450.99
362_T371_Y1.0440.99
53_I133_D1.0360.99
270_G301_S1.0330.99
264_Q287_A1.0240.99
162_V199_L1.0220.99
276_S310_S1.0170.99
317_T341_L1.0140.98
292_F308_A1.0030.98
99_S126_L0.9920.98
135_I159_K0.9910.98
137_V163_I0.9880.98
114_K118_K0.9820.98
268_I282_A0.9780.98
146_E323_R0.9720.98
141_T165_G0.9700.98
121_K258_N0.9700.98
165_G202_L0.9600.98
277_Y281_A0.9580.98
145_E349_Q0.9570.98
56_L137_V0.9560.98
53_I189_G0.9510.98
109_T299_S0.9480.98
207_A230_F0.9470.98
196_K243_E0.9460.98
124_N256_V0.9330.97
201_V234_I0.9330.97
187_V193_A0.9330.97
352_R356_M0.9300.97
54_R101_E0.9280.97
142_D296_G0.9280.97
124_N258_N0.9270.97
149_Y153_L0.9260.97
323_R349_Q0.9250.97
363_N367_K0.9230.97
213_T348_P0.9200.97
97_N133_D0.9200.97
152_N209_A0.9190.97
65_A84_S0.9170.97
143_T295_S0.9160.97
96_A189_G0.9150.97
104_V119_L0.9140.97
270_G292_F0.9140.97
168_R220_T0.9070.97
96_A185_V0.9040.97
274_D304_A0.9000.97
95_I182_Y0.8950.97
62_I177_G0.8950.97
299_S333_Q0.8790.96
362_T367_K0.8780.96
355_L368_I0.8700.96
312_V339_D0.8670.96
270_G295_S0.8630.96
367_K371_Y0.8530.96
55_I138_T0.8490.95
312_V338_K0.8450.95
144_L163_I0.8450.95
193_A199_L0.8440.95
211_Y350_K0.8390.95
187_V234_I0.8340.95
187_V238_I0.8310.95
142_D299_S0.8260.95
149_Y353_M0.8250.95
117_L121_K0.8190.95
132_V159_K0.8160.94
174_S179_S0.8140.94
205_R230_F0.8110.94
113_N272_Q0.8110.94
283_V290_I0.8050.94
197_G231_V0.8020.94
63_A138_T0.8020.94
238_I241_N0.8000.94
90_P182_Y0.7990.94
145_E323_R0.7990.94
203_N221_D0.7980.94
333_Q336_A0.7970.94
58_T139_H0.7950.94
291_V319_V0.7930.94
218_T297_N0.7920.93
149_Y210_R0.7900.93
321_S351_A0.7890.93
215_T325_G0.7830.93
179_S220_T0.7830.93
241_N344_N0.7810.93
270_G304_A0.7800.93
216_N219_T0.7790.93
279_F308_A0.7790.93
270_G300_L0.7780.93
196_K245_T0.7740.93
351_A372_F0.7730.93
257_S361_K0.7730.93
72_T172_A0.7720.93
211_Y345_S0.7670.92
277_Y285_A0.7620.92
190_A240_F0.7620.92
211_Y354_L0.7580.92
51_P133_D0.7570.92
55_I181_L0.7560.92
312_V315_G0.7520.92
274_D301_S0.7520.92
283_V307_G0.7520.92
284_K310_S0.7480.91
213_T347_N0.7440.91
102_Q105_N0.7440.91
113_N117_L0.7410.91
348_P351_A0.7380.91
123_I127_L0.7380.91
306_K309_D0.7370.91
56_L126_L0.7370.91
141_T214_K0.7350.91
346_L354_L0.7350.91
199_L206_I0.7350.91
156_K255_S0.7320.90
153_L354_L0.7320.90
300_L304_A0.7310.90
310_S313_K0.7290.90
91_E178_P0.7250.90
206_I238_I0.7240.90
152_N208_S0.7240.90
268_I278_L0.7230.90
364_D371_Y0.7210.90
217_T328_V0.7210.90
265_V291_V0.7210.90
162_V187_V0.7190.90
132_V135_I0.7180.90
214_K296_G0.7170.90
308_A312_V0.7160.90
170_S175_A0.7150.89
162_V188_A0.7110.89
306_K310_S0.7060.89
90_P96_A0.7050.89
217_T324_T0.7050.89
331_P334_D0.7030.89
137_V151_L0.7030.89
156_K257_S0.7010.89
125_H128_A0.7010.89
295_S301_S0.7000.88
169_P172_A0.6960.88
65_A79_V0.6930.88
216_N327_G0.6920.88
176_D214_K0.6920.88
205_R224_K0.6920.88
289_G351_A0.6880.88
110_N335_Y0.6880.88
347_N350_K0.6880.88
153_L357_L0.6830.87
126_L132_V0.6820.87
107_G110_N0.6800.87
55_I185_V0.6790.87
120_A256_V0.6790.87
163_I209_A0.6780.87
311_A316_V0.6760.87
331_P342_A0.6760.87
320_R331_P0.6750.87
134_G160_P0.6750.87
218_T330_T0.6740.87
54_R99_S0.6740.87
343_S372_F0.6730.86
292_F300_L0.6730.86
232_G240_F0.6720.86
272_Q349_Q0.6720.86
164_V184_A0.6720.86
357_L361_K0.6700.86
208_S211_Y0.6600.85
104_V118_K0.6600.85
66_D70_T0.6590.85
66_D171_T0.6570.85
127_L155_V0.6570.85
276_S303_A0.6550.85
90_P95_I0.6550.85
317_T365_P0.6550.85
109_T336_A0.6540.85
288_K316_V0.6540.85
266_D275_G0.6530.85
294_G300_L0.6530.85
307_G311_A0.6500.84
270_G274_D0.6500.84
305_E338_K0.6490.84
312_V340_L0.6490.84
120_A150_F0.6480.84
283_V286_G0.6480.84
64_G109_T0.6470.84
208_S231_V0.6470.84
62_I166_S0.6460.84
63_A82_V0.6450.84
109_T295_S0.6440.84
114_K314_K0.6410.84
283_V314_K0.6380.83
58_T119_L0.6380.83
288_K363_N0.6380.83
148_A212_V0.6380.83
53_I185_V0.6370.83
56_L123_I0.6330.83
226_E326_N0.6320.83
299_S334_D0.6310.83
123_I155_V0.6290.83
205_R233_T0.6290.83
148_A200_V0.6280.82
289_G321_S0.6280.82
124_N156_K0.6270.82
299_S336_A0.6260.82
116_L147_T0.6260.82
118_K122_R0.6260.82
146_E352_R0.6250.82
296_G299_S0.6250.82
221_D224_K0.6250.82
145_E212_V0.6240.82
62_I138_T0.6230.82
51_P96_A0.6220.82
311_A317_T0.6210.82
54_R123_I0.6210.82
366_Q370_A0.6200.82
67_Q74_E0.6190.82
297_N320_R0.6190.82
302_D306_K0.6180.81
95_I189_G0.6170.81
107_G209_A0.6150.81
83_E99_S0.6140.81
167_M296_G0.6130.81
341_L369_Q0.6090.80
149_Y354_L0.6050.80
124_N157_S0.6050.80
289_G317_T0.6020.80
153_L350_K0.6010.80
167_M202_L0.6000.79
126_L135_I0.5980.79
359_L362_T0.5970.79
213_T325_G0.5960.79
166_S279_F0.5960.79
106_V112_D0.5950.79
284_K314_K0.5950.79
167_M222_T0.5940.79
289_G359_L0.5920.79
68_S73_T0.5890.78
153_L210_R0.5880.78
213_T349_Q0.5860.78
350_K354_L0.5850.78
235_A297_N0.5840.78
343_S351_A0.5840.78
121_K259_L0.5830.78
141_T207_A0.5820.77
57_A64_G0.5800.77
202_L207_A0.5800.77
199_L240_F0.5800.77
350_K353_M0.5790.77
170_S177_G0.5760.77
274_D279_F0.5750.77
276_S279_F0.5750.77
227_E345_S0.5750.77
117_L150_F0.5740.76
103_I273_N0.5740.76
89_V95_I0.5720.76
167_M214_K0.5700.76
127_L132_V0.5700.76
58_T61_T0.5690.76
157_S252_T0.5690.76
186_K237_D0.5690.76
89_V185_V0.5680.76
287_A316_V0.5680.76
118_K125_H0.5680.76
269_Y349_Q0.5680.76
328_V344_N0.5670.76
127_L131_D0.5650.75
364_D370_A0.5630.75
89_V96_A0.5630.75
66_D172_A0.5620.75
120_A155_V0.5610.75
241_N244_I0.5600.75
210_R345_S0.5580.74
117_L259_L0.5580.74
162_V206_I0.5570.74
184_A201_V0.5560.74
263_P288_K0.5560.74
186_K190_A0.5550.74
121_K263_P0.5540.74
192_E196_K0.5540.74
149_Y209_A0.5510.74
92_M96_A0.5510.74
59_G62_I0.5500.73
160_P196_K0.5470.73
138_T177_G0.5460.73
297_N330_T0.5460.73
362_T365_P0.5440.73
56_L135_I0.5410.72
278_L281_A0.5400.72
268_I274_D0.5390.72
225_S229_G0.5390.72
259_L366_Q0.5370.72
279_F290_I0.5370.72
187_V240_F0.5370.72
165_G214_K0.5340.71
329_V342_A0.5330.71
278_L287_A0.5320.71
332_N336_A0.5300.71
183_N237_D0.5290.70
152_N197_G0.5290.70
267_I352_R0.5280.70
305_E309_D0.5250.70
84_S87_E0.5250.70
109_T202_L0.5240.70
138_T197_G0.5240.70
292_F320_R0.5230.70
259_L360_T0.5220.69
99_S133_D0.5210.69
168_R174_S0.5190.69
301_S304_A0.5180.69
317_T359_L0.5170.69
52_N131_D0.5170.69
116_L139_H0.5160.69
271_Y274_D0.5160.69
111_I147_T0.5160.69
303_A306_K0.5160.69
117_L258_N0.5150.68
148_A163_I0.5150.68
330_T333_Q0.5130.68
57_A100_G0.5130.68
98_V136_V0.5120.68
89_V182_Y0.5100.68
74_E79_V0.5080.67
186_K238_I0.5080.67
167_M323_R0.5070.67
120_A151_L0.5060.67
67_Q70_T0.5060.67
199_L232_G0.5060.67
123_I151_L0.5060.67
107_G144_L0.5030.67
108_S139_H0.5000.66
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1zq1A 2 0.976 100 0.093 Contact Map
2d6fA 2 0.9573 100 0.096 Contact Map
3nxkA 4 0.8693 100 0.151 Contact Map
1wsaA 4 0.8453 100 0.153 Contact Map
1o7jA 4 0.864 100 0.154 Contact Map
2wltA 4 0.8613 100 0.154 Contact Map
1agxA 4 0.8667 100 0.156 Contact Map
1nnsA 4 0.8613 100 0.158 Contact Map
4pgaA 4 0.8693 100 0.169 Contact Map
2himA 4 0.8453 100 0.181 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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