YTCC - BACSU - GREMLIN Contacts

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YTCC - Putative glycosyltransferase YtcC

UniProt:	O34413	Sequences: MKLAFICTEKLPAPAVRGGAIQMMIDGVTPYFSSRYDLTIFSIEDPSLPKRETKDGVHYIHLPKEHYREAVAEELRASSFDLIHVFNRPLNVSLYKKASPNSKIVLSLHNEMFSEKKMTFAQGKEVLDNVSMITTVSEFIKQTVIERFPEAEDITKVVYSGVDLNSYPPVWTMKGSAVRKTYRKKYGIEDKKVILFAGRLSPTKGPHLLIHSMRRILQQHPDAVLVIAGGKWFSDDSENQYVTYLRTLALPYRDHIIFTKFIPADDIPNLFLMADVFVCSSQWNEPLARVNYEAMAAGTPLITTNRGGNGEVVKHEVTGLVIDSYNKPSSFAKAIDRAFTDQELMNKMTKNARKHVEALFTFTHAAKRLNTVYQSVLTPKNKQFPPPFLTQNFDLSSINQLFVKAKT	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	407 (345)
Sequences:	52438 (44220)
Seq/√Len:	2380.7

ECOLI - RFAG WCAL GLGA WCAI RFAB WBBK WCAC
BACSU - YTCC COTSA YPJH EPSD EPSF GLGA YQGM TUAC TUAH YKVP CGEB TAGE
HALSA - Q9HQQ0 Q9HSW1 Q9HS45 Q9HRP8 Q9HSV4 Q9HQT2 Q9HSV2 Q9HRP9
SULSO - Q97XT7 Q980C8 Q97ZD3 Q97V61 Q97VL4 Q97UM0 Q97XT8 Q97UU3 Q97XT9 Q97V80 Q97XU2 Q7LXI2 Q97XT5 Q7LXM9 Q97XT6 Q97XT1 Q97XF6 Q7LXF8 Q7LXF5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
195_L	274_A	4.848	1.00
224_V	257_I	3.655	1.00
132_M	156_K	3.267	1.00
238_E	242_V	3.255	1.00
277_F	294_A	3.144	1.00
84_H	373_Y	2.910	1.00
193_V	226_V	2.817	1.00
215_R	332_A	2.787	1.00
355_H	359_L	2.785	1.00
191_K	224_V	2.717	1.00
226_V	259_T	2.672	1.00
196_F	206_P	2.654	1.00
211_H	328_P	2.225	1.00
192_K	339_F	2.170	1.00
141_K	145_I	2.169	1.00
219_Q	336_D	2.162	1.00
39_T	80_F	2.162	1.00
205_G	208_L	2.127	1.00
39_T	78_S	2.099	1.00
211_H	244_Y	2.090	1.00
334_A	337_R	2.048	1.00
305_N	310_G	2.036	1.00
213_M	225_L	1.996	1.00
228_A	270_L	1.994	1.00
36_Y	370_N	1.969	1.00
193_V	224_V	1.965	1.00
226_V	257_I	1.955	1.00
215_R	329_S	1.948	1.00
341_D	344_L	1.938	1.00
4_A	80_F	1.923	1.00
313_V	319_G	1.918	1.00
194_I	276_V	1.892	1.00
337_R	344_L	1.874	1.00
298_G	353_R	1.873	1.00
192_K	275_D	1.849	1.00
237_S	240_Q	1.843	1.00
345_M	349_T	1.829	1.00
193_V	273_M	1.825	1.00
132_M	372_V	1.806	1.00
39_T	60_I	1.805	1.00
318_T	355_H	1.791	1.00
320_L	334_A	1.788	1.00
314_K	317_V	1.778	1.00
364_H	368_R	1.777	1.00
161_G	362_F	1.772	1.00
84_H	107_S	1.734	1.00
323_D	330_S	1.718	1.00
220_H	336_D	1.714	1.00
138_E	141_K	1.713	1.00
305_N	323_D	1.712	1.00
156_K	375_S	1.709	1.00
205_G	282_Q	1.707	1.00
244_Y	250_L	1.700	1.00
336_D	340_T	1.699	1.00
277_F	299_T	1.692	1.00
209_L	225_L	1.687	1.00
230_G	238_E	1.677	1.00
35_R	367_K	1.674	1.00
106_L	130_V	1.672	1.00
35_R	370_N	1.662	1.00
141_K	157_V	1.652	1.00
370_N	374_Q	1.637	1.00
314_K	355_H	1.632	1.00
197_A	267_I	1.627	1.00
337_R	341_D	1.617	1.00
31_Y	366_A	1.566	1.00
263_P	266_D	1.558	1.00
135_T	155_T	1.547	1.00
320_L	337_R	1.544	1.00
138_E	311_E	1.541	1.00
213_M	223_A	1.541	1.00
26_D	30_P	1.524	1.00
31_Y	363_T	1.505	1.00
317_V	351_N	1.505	1.00
209_L	278_V	1.502	1.00
138_E	157_V	1.498	1.00
326_N	330_S	1.488	1.00
330_S	334_A	1.487	1.00
344_L	347_K	1.478	1.00
31_Y	34_S	1.474	1.00
214_R	250_L	1.471	1.00
39_T	58_H	1.462	1.00
347_K	351_N	1.460	1.00
246_R	253_R	1.460	1.00
203_T	283_W	1.448	1.00
266_D	269_N	1.430	1.00
314_K	318_T	1.427	1.00
354_K	358_A	1.420	1.00
303_T	309_N	1.406	1.00
4_A	41_F	1.400	1.00
216_I	336_D	1.400	1.00
228_A	259_T	1.384	1.00
208_L	328_P	1.378	1.00
141_K	155_T	1.377	1.00
85_V	104_I	1.359	1.00
134_T	372_V	1.353	1.00
274_A	299_T	1.320	1.00
262_I	266_D	1.320	1.00
226_V	270_L	1.311	1.00
215_R	328_P	1.309	1.00
214_R	218_Q	1.305	1.00
208_L	282_Q	1.302	1.00
135_T	141_K	1.301	1.00
207_H	244_Y	1.301	1.00
328_P	332_A	1.299	1.00
322_I	334_A	1.296	1.00
268_P	297_A	1.294	1.00
212_S	331_F	1.293	1.00
5_F	32_F	1.289	1.00
193_V	275_D	1.286	1.00
194_I	335_I	1.274	1.00
200_L	227_I	1.273	1.00
5_F	86_F	1.273	1.00
368_R	371_T	1.265	1.00
145_I	152_E	1.263	1.00
134_T	369_L	1.258	1.00
194_I	278_V	1.257	1.00
220_H	340_T	1.254	1.00
196_F	209_L	1.249	1.00
287_L	292_Y	1.244	1.00
195_L	228_A	1.237	1.00
330_S	333_K	1.229	1.00
206_P	227_I	1.226	1.00
132_M	375_S	1.225	1.00
351_N	354_K	1.205	1.00
364_H	367_K	1.205	1.00
226_V	274_A	1.199	1.00
242_V	246_R	1.195	1.00
138_E	142_Q	1.181	1.00
151_A	154_I	1.179	1.00
332_A	336_D	1.172	1.00
167_Y	297_A	1.166	1.00
192_K	276_V	1.159	1.00
279_C	291_N	1.134	1.00
264_A	267_I	1.132	1.00
142_Q	145_I	1.132	1.00
243_T	247_T	1.122	1.00
291_N	309_N	1.120	1.00
307_G	310_G	1.118	1.00
349_T	352_A	1.117	1.00
267_I	271_F	1.111	1.00
24_M	289_R	1.110	1.00
298_G	350_K	1.102	1.00
272_L	297_A	1.102	1.00
362_F	365_A	1.098	1.00
136_V	140_I	1.097	1.00
361_T	364_H	1.096	1.00
282_Q	325_Y	1.091	1.00
135_T	144_V	1.090	1.00
244_Y	247_T	1.085	1.00
31_Y	35_R	1.084	1.00
166_S	268_P	1.082	1.00
346_N	350_K	1.080	1.00
237_S	241_Y	1.077	1.00
207_H	211_H	1.077	1.00
230_G	261_F	1.075	1.00
239_N	243_T	1.069	1.00
365_A	368_R	1.069	1.00
239_N	242_V	1.064	1.00
279_C	290_V	1.058	1.00
136_V	160_S	1.058	1.00
322_I	331_F	1.049	1.00
192_K	223_A	1.048	1.00
344_L	348_M	1.047	1.00
109_H	289_R	1.047	1.00
201_S	232_W	1.040	1.00
281_S	285_E	1.039	1.00
280_S	331_F	1.037	1.00
193_V	274_A	1.035	1.00
208_L	325_Y	1.034	1.00
5_F	29_T	1.033	1.00
338_A	348_M	1.030	1.00
320_L	348_M	1.029	1.00
303_T	321_V	1.029	1.00
299_T	349_T	1.026	1.00
271_F	297_A	1.026	1.00
342_Q	345_M	1.017	1.00
208_L	211_H	1.017	1.00
304_T	322_I	1.017	1.00
96_K	100_P	1.014	1.00
208_L	304_T	1.013	1.00
215_R	219_Q	1.012	1.00
85_V	106_L	1.012	1.00
27_G	362_F	1.011	1.00
315_H	334_A	1.006	1.00
86_F	109_H	1.004	1.00
277_F	290_V	0.998	1.00
156_K	372_V	0.996	1.00
167_Y	268_P	0.996	1.00
195_L	270_L	0.994	1.00
259_T	270_L	0.986	1.00
268_P	272_L	0.983	1.00
27_G	30_P	0.983	1.00
215_R	218_Q	0.982	1.00
191_K	222_D	0.980	1.00
365_A	369_L	0.979	1.00
194_I	213_M	0.979	1.00
333_K	337_R	0.971	1.00
192_K	222_D	0.957	1.00
197_A	228_A	0.956	1.00
320_L	338_A	0.955	1.00
288_A	309_N	0.951	1.00
160_S	289_R	0.948	1.00
367_K	371_T	0.946	1.00
135_T	140_I	0.943	1.00
19_G	22_Q	0.942	1.00
295_M	301_L	0.940	1.00
161_G	365_A	0.934	1.00
197_A	271_F	0.933	1.00
342_Q	346_N	0.928	1.00
285_E	288_A	0.928	1.00
69_E	73_E	0.927	1.00
199_R	203_T	0.926	1.00
308_G	311_E	0.925	1.00
136_V	286_P	0.925	1.00
295_M	352_A	0.924	1.00
228_A	262_I	0.918	1.00
350_K	354_K	0.917	1.00
143_T	146_E	0.916	1.00
164_L	361_T	0.913	1.00
294_A	299_T	0.905	1.00
142_Q	146_E	0.905	1.00
216_I	223_A	0.899	1.00
23_M	27_G	0.899	1.00
196_F	227_I	0.898	1.00
349_T	353_R	0.896	1.00
303_T	313_V	0.896	1.00
216_I	335_I	0.896	1.00
90_L	93_S	0.894	1.00
208_L	331_F	0.892	1.00
209_L	280_S	0.891	1.00
262_I	270_L	0.887	1.00
291_N	303_T	0.886	1.00
320_L	344_L	0.884	1.00
207_H	282_Q	0.882	1.00
29_T	33_S	0.879	1.00
185_K	269_N	0.876	1.00
346_N	349_T	0.874	1.00
24_M	109_H	0.874	1.00
323_D	326_N	0.873	1.00
72_A	99_S	0.871	1.00
224_V	255_H	0.865	1.00
216_I	339_F	0.864	1.00
211_H	250_L	0.863	1.00
29_T	40_I	0.858	1.00
25_I	86_F	0.858	1.00
302_I	322_I	0.855	1.00
204_K	285_E	0.855	1.00
3_L	36_Y	0.854	1.00
223_A	339_F	0.853	1.00
163_D	166_S	0.853	1.00
240_Q	244_Y	0.852	1.00
291_N	301_L	0.852	1.00
213_M	255_H	0.850	1.00
300_P	349_T	0.849	1.00
318_T	352_A	0.846	1.00
130_V	133_I	0.845	1.00
214_R	252_Y	0.844	1.00
226_V	273_M	0.843	1.00
243_T	246_R	0.843	1.00
367_K	370_N	0.838	1.00
212_S	332_A	0.835	1.00
276_V	338_A	0.835	1.00
271_F	299_T	0.835	1.00
105_V	134_T	0.834	1.00
259_T	262_I	0.830	1.00
72_A	76_R	0.828	1.00
348_M	351_N	0.828	1.00
163_D	167_Y	0.826	1.00
134_T	158_V	0.826	1.00
216_I	332_A	0.818	1.00
104_I	130_V	0.818	1.00
295_M	318_T	0.816	1.00
245_L	258_F	0.811	1.00
196_F	280_S	0.809	1.00
139_F	142_Q	0.809	1.00
27_G	163_D	0.808	1.00
156_K	368_R	0.806	1.00
295_M	355_H	0.804	1.00
279_C	309_N	0.802	1.00
65_E	69_E	0.801	1.00
39_T	43_I	0.801	1.00
195_L	271_F	0.799	1.00
333_K	336_D	0.792	1.00
125_E	129_N	0.787	1.00
302_I	334_A	0.783	1.00
306_R	309_N	0.782	1.00
278_V	335_I	0.780	1.00
145_I	155_T	0.778	1.00
355_H	360_F	0.777	1.00
204_K	290_V	0.776	1.00
361_T	365_A	0.776	1.00
329_S	333_K	0.775	1.00
245_L	252_Y	0.775	1.00
24_M	160_S	0.773	1.00
291_N	312_V	0.772	1.00
158_V	372_V	0.769	1.00
313_V	321_V	0.767	1.00
121_A	125_E	0.767	1.00
275_D	345_M	0.764	1.00
19_G	199_R	0.762	1.00
24_M	107_S	0.759	1.00
362_F	366_A	0.757	1.00
97_K	104_I	0.752	1.00
300_P	338_A	0.751	1.00
23_M	264_A	0.751	1.00
212_S	328_P	0.749	1.00
196_F	277_F	0.749	1.00
269_N	272_L	0.746	1.00
211_H	215_R	0.746	1.00
216_I	220_H	0.745	1.00
276_V	335_I	0.743	1.00
198_G	206_P	0.742	1.00
219_Q	332_A	0.741	1.00
363_T	367_K	0.741	1.00
95_Y	99_S	0.741	1.00
204_K	281_S	0.737	1.00
264_A	268_P	0.735	1.00
240_Q	243_T	0.734	1.00
170_V	272_L	0.734	1.00
159_Y	368_R	0.732	1.00
136_V	289_R	0.730	1.00
159_Y	311_E	0.727	1.00
270_L	273_M	0.727	1.00
35_R	363_T	0.726	1.00
84_H	369_L	0.724	1.00
304_T	324_S	0.724	1.00
33_S	36_Y	0.722	1.00
326_N	329_S	0.720	1.00
37_D	58_H	0.720	1.00
143_T	147_R	0.718	1.00
211_H	214_R	0.718	1.00
305_N	321_V	0.716	1.00
353_R	356_V	0.715	1.00
105_V	376_V	0.714	1.00
74_E	78_S	0.712	1.00
202_P	207_H	0.711	1.00
241_Y	245_L	0.709	1.00
27_G	31_Y	0.708	1.00
139_F	143_T	0.708	1.00
109_H	136_V	0.707	1.00
231_K	238_E	0.706	1.00
310_G	321_V	0.706	1.00
369_L	373_Y	0.705	1.00
371_T	375_S	0.705	1.00
33_S	56_G	0.702	1.00
33_S	38_L	0.696	1.00
302_I	338_A	0.694	1.00
372_V	375_S	0.693	1.00
197_A	277_F	0.693	1.00
30_P	55_D	0.693	1.00
196_F	225_L	0.692	1.00
35_R	366_A	0.690	1.00
337_R	340_T	0.687	1.00
269_N	273_M	0.686	1.00
348_M	352_A	0.685	1.00
110_N	203_T	0.684	1.00
185_K	266_D	0.684	1.00
292_Y	360_F	0.684	1.00
347_K	350_K	0.681	1.00
210_I	245_L	0.680	1.00
231_K	237_S	0.679	1.00
121_A	124_K	0.676	1.00
23_M	26_D	0.675	1.00
194_I	339_F	0.675	1.00
194_I	223_A	0.675	1.00
105_V	373_Y	0.674	1.00
132_M	376_V	0.674	1.00
207_H	240_Q	0.669	1.00
315_H	318_T	0.669	1.00
86_F	107_S	0.667	1.00
192_K	342_Q	0.665	1.00
82_L	373_Y	0.665	1.00
371_T	374_Q	0.663	1.00
288_A	291_N	0.663	1.00
279_C	288_A	0.662	1.00
329_S	332_A	0.662	1.00
7_C	25_I	0.658	1.00
124_K	128_D	0.658	1.00
219_Q	333_K	0.655	1.00
220_H	223_A	0.654	1.00
343_E	347_K	0.653	1.00
287_L	360_F	0.653	1.00
207_H	237_S	0.653	1.00
24_M	369_L	0.652	1.00
141_K	152_E	0.651	1.00
158_V	369_L	0.650	1.00
89_P	92_V	0.650	1.00
68_R	72_A	0.647	1.00
238_E	241_Y	0.644	1.00
318_T	351_N	0.643	1.00
202_P	282_Q	0.642	1.00
69_E	72_A	0.642	1.00
164_L	296_A	0.642	1.00
278_V	331_F	0.641	1.00
73_E	77_A	0.640	1.00
103_K	131_S	0.639	1.00
105_V	132_M	0.638	1.00
372_V	376_V	0.632	1.00
156_K	159_Y	0.632	1.00
9_E	22_Q	0.629	1.00
66_H	70_A	0.629	1.00
145_I	149_P	0.628	1.00
324_S	330_S	0.627	1.00
304_T	331_F	0.627	1.00
7_C	21_I	0.624	1.00
159_Y	360_F	0.623	1.00
5_F	38_L	0.618	1.00
338_A	345_M	0.617	1.00
295_M	349_T	0.616	1.00
198_G	204_K	0.609	1.00
313_V	318_T	0.608	1.00
100_P	103_K	0.607	1.00
341_D	345_M	0.603	1.00
158_V	368_R	0.602	1.00
210_I	256_I	0.602	1.00
34_S	363_T	0.600	1.00
316_E	337_R	0.600	1.00
205_G	280_S	0.600	1.00
270_L	274_A	0.599	1.00
107_S	369_L	0.598	1.00
29_T	38_L	0.598	1.00
139_F	310_G	0.595	1.00
31_Y	362_F	0.595	1.00
366_A	370_N	0.592	1.00
202_P	237_S	0.592	1.00
277_F	301_L	0.590	1.00
89_P	93_S	0.590	1.00
315_H	320_L	0.587	1.00
222_D	255_H	0.587	1.00
250_L	253_R	0.587	1.00
30_P	34_S	0.587	1.00
189_E	192_K	0.585	1.00
28_V	369_L	0.584	1.00
352_A	355_H	0.584	1.00
83_I	97_K	0.584	1.00
271_F	290_V	0.582	1.00
216_I	221_P	0.578	1.00
212_S	335_I	0.577	1.00
96_K	99_S	0.577	1.00
36_Y	374_Q	0.576	1.00
343_E	346_N	0.576	1.00
359_L	364_H	0.575	1.00
275_D	342_Q	0.574	1.00
80_F	102_S	0.573	1.00
97_K	100_P	0.572	1.00
205_G	325_Y	0.571	1.00
98_A	129_N	0.569	1.00
209_L	213_M	0.567	1.00
21_I	87_N	0.567	1.00
351_N	355_H	0.566	1.00
192_K	195_L	0.563	1.00
221_P	340_T	0.562	1.00
302_I	335_I	0.561	1.00
247_T	250_L	0.560	1.00
29_T	57_V	0.560	1.00
137_S	140_I	0.559	1.00
195_L	277_F	0.559	1.00
213_M	216_I	0.558	1.00
262_I	267_I	0.557	1.00
200_L	206_P	0.557	1.00
24_M	28_V	0.554	1.00
76_R	100_P	0.554	1.00
276_V	302_I	0.552	1.00
192_K	345_M	0.550	1.00
200_L	238_E	0.549	1.00
55_D	58_H	0.547	1.00
109_H	160_S	0.545	1.00
165_N	168_P	0.543	1.00
229_G	260_K	0.543	1.00
168_P	272_L	0.539	1.00
18_G	261_F	0.539	1.00
322_I	330_S	0.538	1.00
305_N	324_S	0.537	1.00
5_F	373_Y	0.537	1.00
135_T	157_V	0.536	1.00
280_S	304_T	0.536	1.00
75_L	80_F	0.534	1.00
27_G	289_R	0.531	1.00
228_A	267_I	0.530	1.00
290_V	294_A	0.529	1.00
24_M	86_F	0.527	1.00
82_L	105_V	0.527	1.00
74_E	77_A	0.526	1.00
242_V	245_L	0.525	1.00
168_P	171_W	0.525	1.00
220_H	339_F	0.524	1.00
200_L	231_K	0.524	1.00
283_W	286_P	0.523	1.00
108_L	140_I	0.521	1.00
163_D	362_F	0.520	1.00
195_L	299_T	0.517	1.00
138_E	159_Y	0.516	1.00
368_R	372_V	0.515	1.00
42_S	59_Y	0.514	1.00
159_Y	287_L	0.512	1.00
295_M	356_V	0.510	1.00
350_K	353_R	0.510	1.00
221_P	336_D	0.510	1.00
208_L	212_S	0.510	1.00
33_S	57_V	0.508	1.00
166_S	264_A	0.508	1.00
107_S	134_T	0.507	1.00
306_R	310_G	0.505	1.00
83_I	102_S	0.505	1.00
205_G	304_T	0.505	1.00
79_S	102_S	0.504	1.00
276_V	345_M	0.504	1.00
110_N	115_E	0.504	1.00
225_L	256_I	0.504	1.00
164_L	356_V	0.504	1.00
300_P	345_M	0.502	1.00
4_A	39_T	0.502	1.00
292_Y	356_V	0.502	1.00
271_F	277_F	0.501	1.00
328_P	331_F	0.500	1.00
112_M	115_E	0.500	1.00
214_R	251_P	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3c48A	2	0.9091	100	0.202	Contact Map
2r60A	1	0.9189	100	0.203	Contact Map
3froA	3	0.914	100	0.211	Contact Map
1rzuA	1	0.8943	100	0.212	Contact Map
4n9wA	1	0.8428	100	0.213	Contact Map
3okpA	1	0.8796	100	0.227	Contact Map
2qzsA	1	0.887	100	0.229	Contact Map
2x6qA	1	0.8722	100	0.243	Contact Map
3oy2A	2	0.86	100	0.244	Contact Map
2jjmA	4	0.8403	100	0.253	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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