GREMLIN Database
MED - Transcriptional activator protein med
UniProt: O32436 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 317 (306)
Sequences: 3747 (2794)
Seq/√Len: 159.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
306_T312_E4.6921.00
136_F172_Y3.7381.00
294_Q298_Q3.5171.00
91_G100_F3.4791.00
305_K310_T3.4681.00
170_A177_I3.4361.00
262_E266_K3.2741.00
302_K306_T3.0511.00
149_N174_N3.0431.00
201_K228_L2.9681.00
75_I102_L2.7031.00
140_M303_L2.6651.00
79_E106_D2.5791.00
304_I308_N2.5701.00
45_T49_K2.5681.00
151_V170_A2.5481.00
150_Q178_E2.5361.00
146_S149_N2.5161.00
144_H299_R2.5091.00
57_K258_E2.4711.00
184_T215_Y2.4101.00
150_Q180_N2.4081.00
39_N45_T2.3571.00
53_N258_E2.1951.00
299_R302_K2.1751.00
146_S206_D2.1451.00
277_D280_T2.1401.00
145_M293_D2.0631.00
82_H112_F2.0601.00
293_D296_F2.0521.00
52_L55_Q2.0181.00
301_A304_I1.9661.00
138_G285_M1.9391.00
301_A305_K1.9361.00
140_M173_E1.9211.00
297_Q301_A1.9201.00
82_H107_Y1.9011.00
150_Q205_A1.8991.00
154_I219_V1.8701.00
134_M249_T1.8541.00
259_I263_Q1.8531.00
79_E107_Y1.8491.00
141_T300_I1.8141.00
53_N57_K1.7651.00
298_Q302_K1.7431.00
129_F256_A1.7381.00
48_Y52_L1.7331.00
52_L56_S1.7091.00
140_M172_Y1.6991.00
167_I171_K1.6961.00
186_H189_D1.6921.00
172_Y303_L1.6781.00
202_N226_D1.6581.00
284_E304_I1.6571.00
138_G231_I1.6531.00
193_A215_Y1.6371.00
305_K308_N1.6351.00
130_S277_D1.6251.00
89_L110_M1.6241.00
131_G161_P1.6221.00
31_G81_F1.5891.00
38_I64_Y1.5761.00
306_T313_L1.5731.00
145_M296_F1.5131.00
294_Q297_Q1.5121.00
54_I261_A1.5091.00
65_K81_F1.4981.00
194_V225_K1.4741.00
263_Q268_T1.4591.00
278_L308_N1.4381.00
163_V167_I1.4351.00
197_Y208_V1.4261.00
63_Y86_V1.4261.00
194_V198_Q1.4241.00
190_D215_Y1.4131.00
232_G237_Q1.3961.00
143_A146_S1.3771.00
180_N205_A1.3721.00
78_I91_G1.3691.00
281_G284_E1.3631.00
54_I58_Y1.3611.00
14_L17_G1.3471.00
58_Y258_E1.3461.00
31_G86_V1.3331.00
28_E264_F1.3311.00
143_A177_I1.3291.00
299_R303_L1.3281.00
201_K223_I1.3121.00
58_Y262_E1.3101.00
130_S133_A1.2831.00
11_V14_L1.2811.00
306_T310_T1.2801.00
133_A279_N1.2581.00
198_Q202_N1.2521.00
298_Q301_A1.2471.00
79_E83_K1.2391.00
29_K61_D1.2331.00
194_V221_Q1.2271.00
252_N255_K1.2171.00
105_E121_A1.2151.00
29_K86_V1.2111.00
47_G257_Y1.2041.00
137_F300_I1.2031.00
148_T206_D1.2021.00
53_N254_D1.1981.00
27_I61_D1.1761.00
138_G249_T1.1691.00
57_K262_E1.1651.00
150_Q204_G1.1581.00
83_K107_Y1.1561.00
137_F304_I1.1491.00
303_L306_T1.1451.00
73_D76_N1.1421.00
141_T247_T1.1411.00
136_F303_L1.1361.00
152_G205_A1.1281.00
184_T196_L1.1241.00
220_I245_V1.1221.00
18_C21_T1.1161.00
88_L261_A1.1151.00
289_S297_Q1.0961.00
144_H296_F1.0900.99
146_S207_V1.0890.99
49_K52_L1.0880.99
180_N203_E1.0860.99
258_E262_E1.0860.99
134_M283_V1.0850.99
34_F64_Y1.0790.99
101_N105_E1.0770.99
55_Q61_D1.0740.99
79_E103_V1.0730.99
36_D44_G1.0530.99
146_S170_A1.0500.99
97_A117_A1.0460.99
125_T269_L1.0450.99
75_I79_E1.0420.99
78_I103_V1.0420.99
222_Q225_K1.0360.99
75_I103_V1.0320.99
28_E265_N1.0280.99
224_K243_N1.0250.99
247_T286_G1.0230.99
195_K198_Q1.0190.99
30_V88_L1.0190.99
51_L62_V1.0110.99
15_L18_C1.0090.99
7_M10_S1.0050.99
252_N284_E1.0010.99
182_K200_M1.0000.99
259_I262_E0.9970.99
152_G200_M0.9960.99
111_E269_L0.9930.99
198_Q222_Q0.9900.99
143_A173_E0.9900.99
227_G244_T0.9900.99
10_S13_L0.9810.99
229_Y246_L0.9810.99
9_F12_L0.9800.99
79_E82_H0.9790.99
81_F86_V0.9780.99
92_H115_S0.9760.99
48_Y64_Y0.9740.99
38_I48_Y0.9670.99
207_V231_I0.9640.99
219_V230_A0.9640.99
201_K208_V0.9610.99
184_T193_A0.9600.99
230_A244_T0.9530.99
18_C24_K0.9450.99
50_G254_D0.9390.98
121_A124_V0.9320.98
220_I230_A0.9310.98
192_T195_K0.9260.98
67_G77_A0.9150.98
174_N177_I0.9150.98
78_I100_F0.9140.98
85_G110_M0.9120.98
140_M299_R0.9110.98
55_Q62_V0.9100.98
104_G121_A0.9040.98
250_V284_E0.9040.98
101_N124_V0.9020.98
130_S275_Y0.9000.98
255_K259_I0.9000.98
120_K126_S0.8960.98
90_Y113_V0.8950.98
198_Q226_D0.8940.98
88_L264_F0.8930.98
12_L15_L0.8860.98
113_V127_V0.8850.98
189_D192_T0.8810.98
130_S280_T0.8780.98
182_K196_L0.8750.98
229_Y291_L0.8740.98
209_Y249_T0.8700.97
220_I244_T0.8690.97
87_N110_M0.8690.97
140_M144_H0.8670.97
232_G248_S0.8650.97
303_L313_L0.8650.97
88_L113_V0.8620.97
97_A101_N0.8610.97
70_T73_D0.8530.97
82_H110_M0.8530.97
167_I179_V0.8510.97
141_T292_V0.8500.97
305_K312_E0.8500.97
145_M207_V0.8400.97
200_M205_A0.8360.97
135_G249_T0.8360.97
32_M47_G0.8330.97
220_I241_G0.8290.97
223_I230_A0.8290.97
155_A159_W0.8290.97
35_P95_E0.8220.96
30_V261_A0.8220.96
78_I96_Y0.8180.96
36_D40_D0.8070.96
238_S286_G0.8060.96
149_N206_D0.8060.96
133_A277_D0.8020.96
98_E101_N0.7960.96
32_M51_L0.7950.96
100_F124_V0.7950.96
167_I181_T0.7910.96
281_G308_N0.7890.96
7_M11_V0.7870.95
153_V211_A0.7840.95
148_T204_G0.7820.95
302_K305_K0.7780.95
51_L55_Q0.7770.95
82_H103_V0.7740.95
241_G244_T0.7730.95
262_E265_N0.7730.95
230_A245_V0.7730.95
38_I45_T0.7730.95
8_I14_L0.7710.95
144_H173_E0.7690.95
263_Q266_K0.7670.95
295_D298_Q0.7650.95
302_K312_E0.7640.95
34_F48_Y0.7600.95
114_I117_A0.7590.95
212_G216_N0.7580.94
295_D299_R0.7580.94
28_E87_N0.7560.94
156_S213_D0.7550.94
101_N121_A0.7550.94
53_N56_S0.7520.94
231_I247_T0.7500.94
129_F251_Q0.7500.94
146_S177_I0.7460.94
145_M246_L0.7460.94
53_N255_K0.7450.94
169_G172_Y0.7440.94
170_A179_V0.7420.94
111_E264_F0.7420.94
195_K199_K0.7410.94
234_V251_Q0.7330.93
34_F38_I0.7330.93
116_N251_Q0.7320.93
54_I60_V0.7300.93
276_Y280_T0.7270.93
211_A233_Y0.7200.93
141_T231_I0.7180.93
136_F169_G0.7180.93
26_K87_N0.7160.93
288_F291_L0.7150.93
247_T287_T0.7150.93
46_K254_D0.7140.93
137_F278_L0.7140.93
8_I11_V0.7130.92
78_I82_H0.7100.92
143_A174_N0.7090.92
47_G253_V0.7090.92
18_C23_F0.7080.92
48_Y62_V0.7050.92
102_L106_D0.7050.92
143_A149_N0.7050.92
125_T260_I0.7050.92
278_L307_Y0.7040.92
7_M13_L0.7040.92
158_T185_D0.7040.92
210_P219_V0.7030.92
41_L240_L0.7010.92
136_F140_M0.7010.92
154_I197_Y0.7010.92
54_I62_V0.7010.92
140_M170_A0.6980.92
192_T196_L0.6970.92
60_V261_A0.6960.92
50_G54_I0.6960.92
54_I258_E0.6900.91
197_Y201_K0.6890.91
221_Q224_K0.6870.91
90_Y261_A0.6860.91
29_K87_N0.6800.91
35_P93_G0.6780.90
131_G251_Q0.6780.90
159_W162_E0.6770.90
131_G249_T0.6740.90
238_S242_E0.6730.90
233_Y251_Q0.6730.90
135_G209_Y0.6690.90
150_Q203_E0.6690.90
49_K235_T0.6660.90
135_G211_A0.6660.90
141_T145_M0.6660.90
207_V246_L0.6630.89
71_E95_E0.6620.89
127_V260_I0.6600.89
20_Q23_F0.6550.89
115_S253_V0.6530.89
58_Y265_N0.6510.89
180_N200_M0.6510.89
191_T194_V0.6510.89
80_D84_R0.6510.89
288_F292_V0.6490.88
292_V296_F0.6480.88
47_G92_H0.6470.88
15_L19_G0.6470.88
118_K126_S0.6440.88
97_A114_I0.6440.88
281_G304_I0.6420.88
243_N291_L0.6410.88
250_V286_G0.6380.88
222_Q226_D0.6380.88
168_K171_K0.6380.88
224_K244_T0.6380.88
86_V110_M0.6360.87
65_K77_A0.6330.87
149_N176_D0.6320.87
109_D122_D0.6320.87
260_I263_Q0.6310.87
40_D44_G0.6310.87
56_S109_D0.6290.87
103_V106_D0.6290.87
201_K222_Q0.6290.87
245_V248_S0.6290.87
101_N119_A0.6270.87
163_V183_Y0.6260.87
236_D286_G0.6260.87
118_K128_H0.6250.87
140_M300_I0.6250.87
83_K106_D0.6230.86
127_V276_Y0.6230.86
196_L200_M0.6200.86
290_P297_Q0.6200.86
11_V15_L0.6180.86
21_T24_K0.6170.86
7_M17_G0.6170.86
75_I99_V0.6150.86
48_Y51_L0.6130.85
177_I181_T0.6120.85
164_D168_K0.6100.85
292_V297_Q0.6100.85
160_Q163_V0.6090.85
40_D45_T0.6080.85
145_M229_Y0.6080.85
8_I12_L0.6070.85
35_P44_G0.6060.85
135_G162_E0.6060.85
119_A122_D0.6060.85
18_C25_G0.6030.85
113_V260_I0.6030.85
13_L16_S0.6030.85
126_S273_D0.6010.84
125_T270_E0.6000.84
92_H127_V0.5980.84
278_L304_I0.5960.84
264_F269_L0.5940.84
10_S14_L0.5920.83
154_I210_P0.5910.83
74_I96_Y0.5910.83
153_V163_V0.5890.83
255_K258_E0.5870.83
63_Y84_R0.5830.83
55_Q60_V0.5830.83
287_T291_L0.5820.82
163_V181_T0.5810.82
38_I52_L0.5810.82
259_I280_T0.5800.82
151_V177_I0.5790.82
198_Q225_K0.5770.82
138_G247_T0.5760.82
216_N233_Y0.5730.81
49_K254_D0.5710.81
141_T144_H0.5700.81
20_Q24_K0.5690.81
63_Y81_F0.5680.81
14_L18_C0.5620.80
107_Y112_F0.5590.80
285_M300_I0.5580.80
237_Q240_L0.5580.80
78_I112_F0.5580.80
277_D282_V0.5540.79
111_E125_T0.5530.79
166_F209_Y0.5490.79
263_Q270_E0.5480.79
166_F169_G0.5470.78
193_A218_P0.5460.78
214_G217_V0.5440.78
296_F300_I0.5440.78
188_D215_Y0.5430.78
120_K273_D0.5430.78
144_H295_D0.5410.78
54_I265_N0.5380.77
194_V222_Q0.5380.77
172_Y175_P0.5380.77
125_T271_G0.5380.77
73_D77_A0.5360.77
46_K49_K0.5360.77
98_E102_L0.5360.77
172_Y306_T0.5330.77
157_F187_W0.5330.77
247_T288_F0.5330.77
89_L112_F0.5320.76
238_S243_N0.5310.76
298_Q305_K0.5310.76
165_G169_G0.5290.76
125_T263_Q0.5280.76
35_P92_H0.5280.76
97_A119_A0.5270.76
193_A216_N0.5260.76
170_A173_E0.5260.76
171_K175_P0.5250.76
276_Y282_V0.5220.75
16_S19_G0.5210.75
151_V166_F0.5200.75
224_K241_G0.5200.75
115_S257_Y0.5200.75
128_H275_Y0.5200.75
16_S20_Q0.5190.75
29_K63_Y0.5180.75
33_L77_A0.5170.74
87_N109_D0.5170.74
43_W162_E0.5150.74
8_I13_L0.5140.74
9_F16_S0.5120.74
168_K172_Y0.5110.74
146_S151_V0.5100.73
191_T195_K0.5090.73
141_T296_F0.5070.73
212_G215_Y0.5070.73
270_E273_D0.5070.73
210_P216_N0.5070.73
109_D147_K0.5060.73
114_I126_S0.5060.73
43_W116_N0.5060.73
151_V163_V0.5050.73
135_G153_V0.5050.73
132_E161_P0.5050.73
91_G96_Y0.5040.73
119_A124_V0.5030.72
221_Q225_K0.5030.72
48_Y55_Q0.5020.72
162_E233_Y0.5020.72
81_F89_L0.5000.72
243_N290_P0.5000.72
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4pevA 1 0.9117 100 0.377 Contact Map
4iilA 1 0.9148 100 0.399 Contact Map
3s99A 1 0.9054 100 0.41 Contact Map
4p98A 1 0.8991 100 0.414 Contact Map
2fqxA 1 0.9148 100 0.431 Contact Map
2hqbA 1 0.8864 100 0.435 Contact Map
4ycsA 1 0.3785 100 0.61 Contact Map
4wzzA 1 0.8675 99.9 0.713 Contact Map
4yo7A 1 0.8423 99.7 0.758 Contact Map
4z0nA 1 0.8486 99.7 0.762 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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