YWMB - BACSU - GREMLIN Contacts

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YWMB - Uncharacterized protein YwmB

UniProt:	O32277	Sequences: MKKKQVSHAIIISVMLSFVIAVFHTIHASELTPLAQMAEGMERQDVSIDKWTLHAKQNLSLTEKEFYQKVQRLKQEYRQYDWVIAREDKMIKAIGTYTDKKNRTSFRLQLVTTLKKHNPTSYLLYEQMSLETPDSWNDTYEQFERETLGIFQEKVVIFTCLNGHLDDNMNIVLQKKANQLLNEFQARSVEHVVEPNFVSISAFTDEWEEYIMTSKHKMNLQIALRSAGMGGKHTVTVGTPIVTTEY	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	246 (238)
Sequences:	212 (193)
Seq/√Len:	12.5

BACSU - YWMB

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
181_L	202_A	5.541	1.00
201_S	218_M	3.822	0.99
204_T	207_W	3.602	0.99
181_L	186_A	3.394	0.98
122_Y	186_A	2.896	0.95
106_F	125_Y	2.872	0.95
122_Y	163_G	2.763	0.94
190_E	201_S	2.721	0.93
194_E	225_R	2.704	0.93
213_T	218_M	2.474	0.90
160_C	168_N	2.474	0.90
238_G	245_E	2.465	0.89
194_E	197_F	2.433	0.89
201_S	225_R	2.411	0.88
82_W	95_G	2.301	0.86
218_M	241_I	2.259	0.85
186_A	200_I	2.207	0.83
197_F	225_R	2.151	0.81
157_I	160_C	2.129	0.81
51_W	158_F	2.049	0.78
160_C	241_I	2.022	0.77
236_T	245_E	2.013	0.77
167_D	226_S	1.994	0.76
186_A	202_A	1.928	0.74
210_Y	217_K	1.893	0.72
11_I	16_L	1.868	0.71
125_Y	194_E	1.860	0.71
75_Q	161_L	1.834	0.70
195_P	220_L	1.805	0.68
177_A	202_A	1.802	0.68
184_F	245_E	1.799	0.68
199_S	242_V	1.725	0.65
130_L	235_V	1.717	0.64
82_W	194_E	1.708	0.64
221_Q	243_T	1.673	0.62
221_Q	236_T	1.661	0.62
123_L	220_L	1.661	0.62
125_Y	197_F	1.637	0.60
51_W	207_W	1.602	0.59
240_P	246_Y	1.584	0.58
168_N	242_V	1.580	0.58
199_S	245_E	1.571	0.57
158_F	194_E	1.550	0.56
204_T	208_E	1.545	0.56
33_P	207_W	1.543	0.56
201_S	213_T	1.538	0.56
125_Y	173_L	1.531	0.55
199_S	225_R	1.518	0.55
219_N	240_P	1.510	0.54
34_L	37_M	1.491	0.53
80_Y	95_G	1.479	0.53
56_K	125_Y	1.473	0.52
51_W	184_F	1.456	0.51
220_L	237_V	1.453	0.51
184_F	221_Q	1.449	0.51
163_G	199_S	1.416	0.49
51_W	125_Y	1.416	0.49
70_V	187_R	1.415	0.49
106_F	158_F	1.411	0.49
221_Q	245_E	1.409	0.49
163_G	186_A	1.395	0.48
238_G	241_I	1.384	0.48
197_F	202_A	1.382	0.48
65_E	74_K	1.381	0.47
218_M	238_G	1.379	0.47
56_K	158_F	1.372	0.47
51_W	194_E	1.372	0.47
223_A	246_Y	1.371	0.47
125_Y	158_F	1.363	0.47
190_E	221_Q	1.353	0.46
223_A	242_V	1.347	0.46
45_D	237_V	1.337	0.45
66_F	103_R	1.330	0.45
236_T	243_T	1.317	0.44
190_E	243_T	1.288	0.43
49_D	164_H	1.277	0.42
56_K	153_E	1.274	0.42
50_K	162_N	1.267	0.42
126_E	164_H	1.262	0.41
43_R	180_L	1.261	0.41
35_A	237_V	1.236	0.40
46_V	199_S	1.235	0.40
196_N	227_A	1.227	0.40
188_S	191_H	1.224	0.40
184_F	220_L	1.217	0.39
221_Q	238_G	1.216	0.39
190_E	199_S	1.216	0.39
106_F	194_E	1.216	0.39
230_G	234_T	1.209	0.39
13_S	19_V	1.184	0.38
11_I	223_A	1.183	0.38
201_S	243_T	1.176	0.37
238_G	246_Y	1.169	0.37
51_W	129_S	1.147	0.36
51_W	132_T	1.142	0.36
201_S	245_E	1.130	0.35
58_N	83_V	1.127	0.35
41_M	46_V	1.112	0.34
207_W	236_T	1.108	0.34
160_C	167_D	1.099	0.34
205_D	224_L	1.085	0.33
53_L	200_I	1.082	0.33
187_R	222_I	1.079	0.33
88_D	200_I	1.078	0.33
153_E	237_V	1.074	0.33
121_S	177_A	1.068	0.32
51_W	173_L	1.061	0.32
54_H	184_F	1.044	0.31
139_T	199_S	1.038	0.31
67_Y	84_I	1.033	0.31
221_Q	234_T	1.029	0.31
199_S	246_Y	1.028	0.30
9_A	21_A	1.027	0.30
41_M	68_Q	1.025	0.30
160_C	166_D	1.017	0.30
6_V	65_E	1.017	0.30
108_L	150_I	1.012	0.30
30_E	142_Q	1.009	0.30
76_E	200_I	1.001	0.29
125_Y	225_R	0.996	0.29
125_Y	243_T	0.996	0.29
25_T	29_S	0.991	0.29
64_K	95_G	0.990	0.29
241_I	245_E	0.987	0.29
13_S	210_Y	0.975	0.28
145_R	223_A	0.971	0.28
238_G	243_T	0.969	0.28
211_I	222_I	0.964	0.28
173_L	221_Q	0.963	0.28
218_M	243_T	0.962	0.28
9_A	223_A	0.954	0.27
197_F	201_S	0.952	0.27
62_T	65_E	0.949	0.27
46_V	207_W	0.945	0.27
199_S	238_G	0.943	0.27
117_H	200_I	0.939	0.27
74_K	77_Y	0.927	0.26
191_H	201_S	0.915	0.26
182_N	211_I	0.909	0.26
82_W	202_A	0.903	0.25
13_S	18_F	0.897	0.25
176_K	238_G	0.896	0.25
80_Y	231_G	0.894	0.25
139_T	243_T	0.893	0.25
51_W	56_K	0.888	0.25
221_Q	241_I	0.887	0.25
112_T	210_Y	0.885	0.25
19_V	23_F	0.884	0.25
106_F	197_F	0.883	0.25
40_G	231_G	0.877	0.24
190_E	225_R	0.877	0.24
45_D	223_A	0.876	0.24
161_L	165_L	0.873	0.24
225_R	242_V	0.872	0.24
73_L	77_Y	0.869	0.24
203_F	219_N	0.867	0.24
56_K	236_T	0.866	0.24
123_L	167_D	0.864	0.24
153_E	158_F	0.863	0.24
165_L	235_V	0.863	0.24
11_I	102_N	0.862	0.24
159_T	235_V	0.861	0.24
145_R	181_L	0.855	0.24
200_I	226_S	0.853	0.23
228_G	245_E	0.852	0.23
15_M	24_H	0.851	0.23
55_A	164_H	0.850	0.23
188_S	207_W	0.844	0.23
79_Q	153_E	0.843	0.23
14_V	179_Q	0.842	0.23
218_M	236_T	0.842	0.23
241_I	246_Y	0.840	0.23
199_S	243_T	0.839	0.23
198_V	204_T	0.836	0.23
46_V	165_L	0.835	0.23
238_G	242_V	0.835	0.23
164_H	232_K	0.835	0.23
11_I	144_E	0.834	0.23
149_G	230_G	0.831	0.23
38_A	184_F	0.824	0.22
189_V	213_T	0.820	0.22
139_T	201_S	0.819	0.22
49_D	135_S	0.818	0.22
88_D	222_I	0.816	0.22
62_T	157_I	0.812	0.22
51_W	106_F	0.808	0.22
15_M	25_T	0.808	0.22
86_R	215_K	0.807	0.22
123_L	144_E	0.805	0.22
160_C	169_M	0.797	0.21
57_Q	153_E	0.795	0.21
192_V	242_V	0.795	0.21
71_Q	201_S	0.792	0.21
85_A	149_G	0.791	0.21
125_Y	172_V	0.790	0.21
110_L	198_V	0.787	0.21
16_L	20_I	0.782	0.21
157_I	199_S	0.778	0.21
202_A	219_N	0.771	0.21
34_L	62_T	0.768	0.20
34_L	40_G	0.765	0.20
89_K	94_I	0.764	0.20
99_D	195_P	0.763	0.20
39_E	136_W	0.761	0.20
127_Q	201_S	0.760	0.20
21_A	116_K	0.760	0.20
129_S	221_Q	0.756	0.20
23_F	110_L	0.754	0.20
196_N	220_L	0.752	0.20
159_T	174_Q	0.750	0.20
54_H	207_W	0.739	0.19
135_S	141_E	0.737	0.19
207_W	221_Q	0.735	0.19
172_V	238_G	0.732	0.19
225_R	234_T	0.730	0.19
28_A	152_Q	0.726	0.19
48_I	51_W	0.725	0.19
134_D	137_N	0.723	0.19
121_S	199_S	0.717	0.19
56_K	121_S	0.713	0.19
46_V	125_Y	0.710	0.19
158_F	241_I	0.708	0.19
43_R	182_N	0.707	0.18
33_P	239_T	0.706	0.18
49_D	111_V	0.704	0.18
124_L	224_L	0.704	0.18
41_M	136_W	0.700	0.18
239_T	246_Y	0.699	0.18
81_D	209_E	0.695	0.18
121_S	124_L	0.695	0.18
16_L	214_S	0.687	0.18
204_T	223_A	0.678	0.18
80_Y	92_K	0.677	0.18
129_S	238_G	0.676	0.18
229_M	244_T	0.673	0.17
126_E	224_L	0.668	0.17
155_V	163_G	0.668	0.17
56_K	228_G	0.667	0.17
177_A	181_L	0.664	0.17
201_S	211_I	0.663	0.17
37_M	161_L	0.662	0.17
159_T	168_N	0.662	0.17
46_V	121_S	0.661	0.17
226_S	234_T	0.661	0.17
68_Q	170_N	0.660	0.17
54_H	201_S	0.658	0.17
125_Y	192_V	0.658	0.17
202_A	213_T	0.651	0.17
168_N	202_A	0.648	0.17
10_I	166_D	0.646	0.17
123_L	209_E	0.645	0.17
201_S	242_V	0.640	0.16
223_A	240_P	0.638	0.16
31_L	202_A	0.638	0.16
58_N	77_Y	0.635	0.16
51_W	197_F	0.635	0.16
48_I	117_H	0.628	0.16
199_S	202_A	0.626	0.16
148_L	200_I	0.623	0.16
92_K	204_T	0.622	0.16
207_W	238_G	0.617	0.16
158_F	229_M	0.616	0.16
137_N	231_G	0.613	0.16
184_F	238_G	0.612	0.16
15_M	43_R	0.609	0.16
93_A	237_V	0.606	0.16
74_K	82_W	0.606	0.16
59_L	88_D	0.604	0.16
203_F	217_K	0.600	0.15
41_M	45_D	0.599	0.15
157_I	223_A	0.599	0.15
33_P	204_T	0.596	0.15
69_K	154_K	0.596	0.15
125_Y	201_S	0.595	0.15
129_S	173_L	0.595	0.15
8_H	48_I	0.592	0.15
89_K	211_I	0.591	0.15
53_L	85_A	0.589	0.15
173_L	243_T	0.589	0.15
145_R	183_E	0.587	0.15
56_K	132_T	0.586	0.15
124_L	181_L	0.585	0.15
106_F	129_S	0.585	0.15
52_T	162_N	0.583	0.15
22_V	165_L	0.583	0.15
12_I	208_E	0.581	0.15
53_L	203_F	0.580	0.15
227_A	230_G	0.580	0.15
10_I	134_D	0.579	0.15
41_M	208_E	0.578	0.15
11_I	87_E	0.577	0.15
218_M	221_Q	0.577	0.15
125_Y	236_T	0.575	0.15
89_K	213_T	0.575	0.15
42_E	61_L	0.574	0.15
34_L	51_W	0.572	0.15
168_N	200_I	0.572	0.15
225_R	245_E	0.570	0.15
59_L	214_S	0.566	0.15
34_L	125_Y	0.566	0.15
111_V	203_F	0.564	0.14
18_F	196_N	0.562	0.14
37_M	77_Y	0.560	0.14
50_K	110_L	0.558	0.14
25_T	28_A	0.554	0.14
167_D	234_T	0.554	0.14
84_I	142_Q	0.553	0.14
11_I	19_V	0.553	0.14
37_M	204_T	0.553	0.14
135_S	145_R	0.552	0.14
92_K	239_T	0.547	0.14
190_E	211_I	0.546	0.14
6_V	93_A	0.545	0.14
56_K	245_E	0.544	0.14
40_G	163_G	0.544	0.14
186_A	190_E	0.543	0.14
191_H	202_A	0.541	0.14
105_S	203_F	0.540	0.14
201_S	244_T	0.540	0.14
106_F	239_T	0.539	0.14
69_K	136_W	0.539	0.14
54_H	122_Y	0.536	0.14
228_G	236_T	0.535	0.14
173_L	184_F	0.533	0.14
8_H	14_V	0.532	0.14
10_I	13_S	0.531	0.14
116_K	184_F	0.531	0.14
39_E	121_S	0.530	0.14
54_H	242_V	0.530	0.14
11_I	237_V	0.529	0.14
55_A	147_T	0.525	0.13
51_W	229_M	0.525	0.13
146_E	227_A	0.524	0.13
192_V	238_G	0.523	0.13
66_F	70_V	0.519	0.13
119_P	203_F	0.518	0.13
52_T	127_Q	0.517	0.13
137_N	147_T	0.516	0.13
171_I	231_G	0.515	0.13
137_N	140_Y	0.515	0.13
198_V	225_R	0.514	0.13
72_R	150_I	0.514	0.13
156_V	238_G	0.514	0.13
177_A	235_V	0.513	0.13
65_E	239_T	0.512	0.13
66_F	202_A	0.509	0.13
76_E	87_E	0.509	0.13
11_I	145_R	0.508	0.13
219_N	246_Y	0.508	0.13
26_I	120_T	0.507	0.13
48_I	238_G	0.507	0.13
96_T	125_Y	0.503	0.13
18_F	34_L	0.503	0.13
129_S	245_E	0.502	0.13
170_N	202_A	0.501	0.13
64_K	138_D	0.501	0.13
169_M	221_Q	0.500	0.13

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2fpnA	2	0.8333	100	0.207	Contact Map
4gycB	2	0.0976	34.6	0.942	Contact Map
1l7bA	1	0.2967	22	0.948	Contact Map
2f95B	2	0.0813	16.9	0.95	Contact Map
2nteA	1	0.2764	12.8	0.953	Contact Map
4cq4A	4	0.3333	12.4	0.953	Contact Map
2etxA	2	0.252	10.3	0.955	Contact Map
2qguA	1	0.6057	9.9	0.955	Contact Map
4fybB	1	0.561	9.1	0.956	Contact Map
4nv5A	1	0.5	9	0.956	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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