GREMLIN Database
TKRA - Probable 2-ketogluconate reductase
UniProt: O32264 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 325 (286)
Sequences: 19415 (13291)
Seq/√Len: 785.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
222_G225_E4.2451.00
223_E251_R3.2041.00
245_D248_A2.9411.00
151_L167_A2.6651.00
254_Q280_L2.5041.00
150_T174_E2.5021.00
213_L242_K2.4161.00
47_E71_K2.3921.00
198_D228_L2.3441.00
150_T176_L2.2201.00
231_N257_W2.1671.00
201_L225_E2.1641.00
152_G200_L2.1531.00
176_L203_Q2.1221.00
202_E228_L2.0751.00
156_M177_Y2.0531.00
125_D129_R2.0341.00
219_H267_E2.0001.00
61_R90_E1.9591.00
111_F167_A1.9571.00
310_A315_Q1.9431.00
196_E199_T1.9291.00
227_K257_W1.9101.00
120_R123_E1.8891.00
251_R255_E1.8681.00
162_Q165_R1.8651.00
118_A206_F1.8631.00
152_G204_S1.8471.00
72_V96_T1.8361.00
61_R65_E1.7921.00
112_S121_V1.7861.00
219_H245_D1.7821.00
174_E193_K1.7731.00
114_I206_F1.7661.00
233_A258_I1.7411.00
221_I226_F1.7351.00
198_D225_E1.7291.00
234_I260_G1.7051.00
254_Q276_P1.7041.00
250_I280_L1.6961.00
94_V320_L1.6861.00
111_F171_F1.6801.00
223_E227_K1.6731.00
235_F244_V1.6721.00
226_F252_A1.6661.00
45_E69_K1.6451.00
113_L262_G1.6261.00
280_L283_V1.6251.00
87_A90_E1.6181.00
199_T203_Q1.5921.00
116_S122_A1.5841.00
36_S62_E1.5651.00
153_I160_G1.5621.00
104_D147_H1.5391.00
246_E267_E1.5341.00
221_I225_E1.5321.00
151_L173_M1.5311.00
205_D230_K1.5241.00
213_L218_Y1.5231.00
65_E91_R1.5161.00
309_L313_Q1.5141.00
109_L290_G1.5041.00
215_D218_Y1.5011.00
149_Q295_K1.5001.00
229_M233_A1.4901.00
144_I149_Q1.4851.00
224_R228_L1.4791.00
250_I254_Q1.4711.00
249_L261_A1.4701.00
117_S260_G1.4391.00
201_L221_I1.4371.00
35_P38_V1.4361.00
36_S60_N1.4191.00
167_A173_M1.4001.00
126_R129_R1.3981.00
223_E248_A1.3951.00
163_A210_I1.3921.00
218_Y242_K1.3911.00
225_E228_L1.3861.00
108_D166_R1.3801.00
162_Q166_R1.3771.00
145_D299_N1.3751.00
234_I259_R1.3751.00
174_E203_Q1.3731.00
40_F66_H1.3521.00
110_A163_A1.3501.00
176_L195_A1.3431.00
88_M95_G1.3421.00
209_L243_T1.3391.00
310_A318_K1.3311.00
48_G72_V1.3241.00
239_S243_T1.3181.00
104_D162_Q1.3111.00
210_I238_I1.2931.00
126_R130_A1.2861.00
153_I208_L1.2841.00
127_F132_K1.2741.00
43_L67_A1.2711.00
64_L91_R1.2651.00
306_E310_A1.2591.00
60_N63_L1.2541.00
209_L235_F1.2461.00
110_A238_I1.2431.00
61_R87_A1.2431.00
302_K306_E1.2401.00
211_T243_T1.2381.00
47_E69_K1.2371.00
200_L204_S1.2271.00
153_I164_A1.2261.00
117_S234_I1.2261.00
193_K203_Q1.2181.00
165_R172_D1.2041.00
109_L113_L1.1991.00
122_A125_D1.1871.00
266_Y285_L1.1761.00
154_I211_T1.1721.00
86_E90_E1.1681.00
198_D224_R1.1631.00
224_R227_K1.1551.00
311_A317_P1.1541.00
154_I220_M1.1491.00
72_V311_A1.1401.00
156_M161_E1.1351.00
87_A91_R1.1311.00
131_G279_Q1.1301.00
154_I221_I1.1291.00
117_S262_G1.1241.00
176_L204_S1.1211.00
115_L171_F1.1181.00
306_E318_K1.1171.00
64_L88_M1.1171.00
75_N83_F1.1161.00
107_A166_R1.1131.00
206_F234_I1.1091.00
303_Q319_N1.1051.00
83_F88_M1.1051.00
163_A208_L1.1011.00
197_L225_E1.1001.00
230_K233_A1.0921.00
104_D166_R1.0901.00
207_I229_M1.0831.00
247_K276_P1.0771.00
117_S284_T1.0731.00
150_T203_Q1.0691.00
114_I151_L1.0641.00
112_S115_L1.0621.00
220_M243_T1.0611.00
65_E87_A1.0591.00
74_S307_N1.0561.00
86_E89_K1.0451.00
271_V274_D1.0421.00
208_L238_I1.0401.00
109_L286_L1.0391.00
222_G248_A1.0371.00
253_L283_V1.0371.00
102_L291_S1.0331.00
246_E275_N1.0201.00
232_S257_W1.0161.00
268_K272_T1.0091.00
114_I167_A1.0031.00
252_A257_W0.9991.00
154_I207_I0.9981.00
226_F235_F0.9951.00
254_Q279_Q0.9911.00
180_R216_E0.9901.00
197_L201_L0.9891.00
36_S40_F0.9841.00
38_V41_E0.9771.00
165_R168_K0.9741.00
154_I197_L0.9721.00
74_S304_A0.9711.00
298_F302_K0.9691.00
109_L262_G0.9641.00
118_A144_I0.9581.00
246_E268_K0.9521.00
168_K191_G0.9471.00
250_I276_P0.9461.00
94_V317_P0.9451.00
252_A258_I0.9351.00
182_R194_Y0.9331.00
84_D87_A0.9321.00
163_A167_A0.9311.00
195_A199_T0.9231.00
85_I89_K0.9201.00
118_A149_Q0.9201.00
29_W46_A0.9191.00
41_E44_K0.9121.00
204_S207_I0.9111.00
182_R187_E0.9101.00
111_F166_R0.9021.00
48_G308_M0.9011.00
179_N183_K0.9001.00
222_G245_D0.8981.00
113_L284_T0.8981.00
221_I235_F0.8941.00
83_F95_G0.8891.00
147_H172_D0.8881.00
161_E183_K0.8851.00
211_T220_M0.8841.00
212_P217_T0.8841.00
75_N82_N0.8831.00
154_I209_L0.8801.00
74_S96_T0.8771.00
151_L208_L0.8761.00
161_E186_T0.8731.00
44_K66_H0.8651.00
307_N310_A0.8601.00
126_R281_D0.8591.00
105_T145_D0.8531.00
116_S121_V0.8511.00
195_A203_Q0.8501.00
226_F229_M0.8501.00
195_A200_L0.8501.00
244_V249_L0.8461.00
48_G312_I0.8401.00
153_I163_A0.8381.00
145_D293_T0.8381.00
122_A126_R0.8351.00
118_A234_I0.8351.00
116_S284_T0.8341.00
77_S82_N0.8301.00
226_F257_W0.8291.00
164_A175_V0.8251.00
209_L221_I0.8221.00
111_F163_A0.8211.00
103_D162_Q0.8201.00
40_F63_L0.8191.00
251_R254_Q0.8091.00
156_M164_A0.8091.00
144_I295_K0.8071.00
183_K186_T0.8051.00
177_Y192_V0.8021.00
201_L207_I0.8021.00
268_K271_V0.8011.00
260_G284_T0.8001.00
247_K251_R0.8001.00
38_V42_K0.7961.00
271_V275_N0.7951.00
50_L304_A0.7911.00
198_D202_E0.7861.00
232_S259_R0.7821.00
106_V290_G0.7801.00
144_I294_A0.7791.00
73_V88_M0.7771.00
72_V308_M0.7751.00
62_E65_E0.7721.00
249_L258_I0.7681.00
64_L73_V0.7661.00
104_D108_D0.7631.00
112_S116_S0.7611.00
261_A283_V0.7591.00
104_D145_D0.7551.00
110_A210_I0.7551.00
246_E277_L0.7511.00
177_Y194_Y0.7491.00
207_I233_A0.7481.00
247_K267_E0.7471.00
103_D158_R0.7471.00
178_H197_L0.7461.00
299_N302_K0.7451.00
199_T202_E0.7451.00
263_L283_V0.7451.00
227_K255_E0.7441.00
146_V173_M0.7431.00
223_E257_W0.7431.00
118_A122_A0.7411.00
187_E194_Y0.7391.00
27_E46_A0.7361.00
110_A208_L0.7341.00
114_I163_A0.7291.00
98_T303_Q0.7281.00
201_L228_L0.7241.00
111_F114_I0.7201.00
49_L59_I0.7191.00
100_Y158_R0.7171.00
27_E42_K0.7161.00
37_D41_E0.7161.00
124_L289_I0.7141.00
166_R171_F0.7141.00
61_R91_R0.7121.00
61_R84_D0.7111.00
75_N80_Y0.7101.00
27_E30_Q0.7091.00
147_H299_N0.7081.00
244_V261_A0.7061.00
150_T204_S0.7051.00
213_L217_T0.7011.00
144_I232_S0.7011.00
43_L49_L0.7001.00
40_F62_E0.6991.00
197_L220_M0.6991.00
80_Y85_I0.6981.00
266_Y271_V0.6971.00
107_A162_Q0.6941.00
251_R257_W0.6901.00
105_T296_V0.6871.00
108_D119_R0.6851.00
253_L282_N0.6851.00
201_L229_M0.6841.00
184_Q187_E0.6821.00
272_T275_N0.6821.00
114_I146_V0.6801.00
209_L239_S0.6801.00
149_Q206_F0.6741.00
173_M206_F0.6691.00
305_A309_L0.6671.00
74_S308_M0.6651.00
316_T320_L0.6631.00
234_I262_G0.6621.00
294_A298_F0.6611.00
192_V195_A0.6601.00
44_K68_P0.6591.00
218_Y267_E0.6561.00
207_I230_K0.6561.00
179_N182_R0.6531.00
98_T300_M0.6521.00
306_E309_L0.6491.00
110_A113_L0.6481.00
128_V278_L0.6471.00
100_Y303_Q0.6441.00
125_D128_V0.6381.00
191_G195_A0.6351.00
150_T230_K0.6351.00
161_E165_R0.6341.00
150_T205_D0.6331.00
232_S258_I0.6331.00
127_F130_A0.6331.00
62_E66_H0.6321.00
71_K311_A0.6321.00
249_L263_L0.6291.00
114_I208_L0.6291.00
51_T59_I0.6281.00
278_L285_L0.6281.00
45_E68_P0.6271.00
310_A316_T0.6271.00
74_S98_T0.6261.00
152_G178_H0.6261.00
67_A70_L0.6251.00
114_I236_V0.6241.00
310_A317_P0.6211.00
105_T108_D0.6191.00
176_L200_L0.6181.00
211_T239_S0.6151.00
223_E255_E0.6141.00
81_D213_L0.6131.00
26_Y29_W0.6101.00
80_Y97_H0.6101.00
100_Y103_D0.6071.00
124_L287_P0.6051.00
214_T217_T0.6051.00
125_D285_L0.6031.00
59_I64_L0.6021.00
271_V278_L0.6011.00
223_E252_A0.6001.00
262_G284_T0.6001.00
68_P91_R0.5991.00
25_R30_Q0.5991.00
166_R170_G0.5991.00
151_L163_A0.5981.00
253_L261_A0.5971.00
156_M194_Y0.5961.00
246_E250_I0.5951.00
25_R29_W0.5951.00
242_K267_E0.5911.00
185_E189_S0.5891.00
113_L116_S0.5871.00
252_A255_E0.5871.00
109_L119_R0.5871.00
177_Y187_E0.5841.00
219_H242_K0.5821.00
104_D172_D0.5821.00
168_K175_V0.5811.00
292_A297_R0.5801.00
132_K135_T0.5761.00
48_G74_S0.5711.00
317_P320_L0.5701.00
105_T293_T0.5691.00
219_H222_G0.5691.00
149_Q173_M0.5681.00
254_Q281_D0.5661.00
146_V171_F0.5651.00
207_I221_I0.5571.00
109_L112_S0.5571.00
37_D62_E0.5521.00
76_Q300_M0.5511.00
156_M179_N0.5511.00
274_D278_L0.5491.00
101_T303_Q0.5491.00
127_F141_L0.5471.00
113_L117_S0.5461.00
184_Q194_Y0.5461.00
106_V110_A0.5461.00
123_E127_F0.5431.00
91_R94_V0.5431.00
176_L193_K0.5421.00
302_K305_A0.5411.00
274_D279_Q0.5401.00
116_S125_D0.5391.00
118_A173_M0.5371.00
211_T217_T0.5361.00
40_F67_A0.5331.00
64_L93_V0.5291.00
165_R170_G0.5281.00
102_L105_T0.5271.00
303_Q307_N0.5271.00
122_A284_T0.5271.00
201_L220_M0.5251.00
240_R288_H0.5251.00
101_T291_S0.5251.00
309_L315_Q0.5241.00
154_I200_L0.5241.00
127_F134_G0.5241.00
144_I259_R0.5231.00
101_T300_M0.5231.00
51_T75_N0.5231.00
118_A146_V0.5221.00
164_A177_Y0.5211.00
129_R279_Q0.5181.00
42_K46_A0.5171.00
304_A307_N0.5161.00
303_Q306_E0.5141.00
127_F140_A0.5141.00
128_V266_Y0.5131.00
34_L38_V0.5131.00
149_Q205_D0.5081.00
217_T243_T0.5081.00
81_D242_K0.5061.00
231_N252_A0.5011.00
128_V285_L0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1ygyA 4 0.9692 100 0.192 Contact Map
2g76A 2 0.9292 100 0.198 Contact Map
3ba1A 2 0.96 100 0.198 Contact Map
2d0iA 2 0.9754 100 0.199 Contact Map
4g2nA 3 0.9723 100 0.202 Contact Map
3wwyA 4 0.9723 100 0.207 Contact Map
3k5pA 3 0.9569 100 0.207 Contact Map
4lswA 2 0.9723 100 0.211 Contact Map
1sc6A 4 0.9323 100 0.212 Contact Map
2gcgA 2 0.9815 100 0.213 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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