YVBT - BACSU - GREMLIN Contacts

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YVBT - Uncharacterized protein YvbT

UniProt:	O32254	Sequences: MSNNQRKDTLLSVLNLSPVVQGGTIAESFRNSMDLARRAEEWGYHRYWLAEHHNIEGVASSATAVLIGHIAGGTKKIRVGSGGIMLPNHSSLVIAEQFGTLETLYPGRIDLGLGRAPGTDQLTARALRRNINSGEDFPEQLEELRNYFKPSGNVRNQVRAIPGEGIDVPIWLLGSSGFSARLAGELGLPFAFAAHFSPANTVPALELYRNSFTPSDVLDEPYAMVGVTIIAADTNEKAQHLATSHYQRFLDLVRGTPNQLKPPVEDMDQIWSPYEKAMVNEQLSSTIVGGPEEVKAKLEDFVKTTQADEIMVNSETFEHADRMRSFEIIADVWKNR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	336 (295)
Sequences:	16442 (10491)
Seq/√Len:	610.8

ECOLI - YHBW RUTA SSUD
BACSU - YVBT YDDN YCEB YTMO YWCH SSUD YXEK MOXC
HALSA - Q9HQZ9
SULSO - Q97Y17

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
235_N	293_E	5.361	1.00
37_R	73_G	3.405	1.00
78_R	110_D	2.805	1.00
235_N	290_G	2.787	1.00
38_R	41_E	2.689	1.00
138_P	185_E	2.677	1.00
297_K	300_D	2.562	1.00
40_E	76_K	2.558	1.00
234_T	237_K	2.546	1.00
206_E	210_N	2.501	1.00
181_R	211_S	2.493	1.00
71_A	105_Y	2.471	1.00
235_N	239_Q	2.436	1.00
67_I	79_V	2.385	1.00
38_R	327_E	2.357	1.00
290_G	293_E	2.339	1.00
180_A	207_L	2.238	1.00
232_A	237_K	2.236	1.00
299_E	303_K	2.233	1.00
142_E	186_L	2.221	1.00
210_N	214_P	2.186	1.00
37_R	41_E	2.172	1.00
30_R	106_P	2.149	1.00
205_L	209_R	2.143	1.00
212_F	221_P	2.127	1.00
33_M	37_R	2.105	1.00
205_L	223_A	2.095	1.00
36_A	70_I	2.043	1.00
207_L	211_S	2.014	1.00
149_K	167_D	1.955	1.00
47_Y	77_I	1.940	1.00
236_E	240_H	1.937	1.00
202_V	206_E	1.926	1.00
34_D	37_R	1.919	1.00
102_E	107_G	1.888	1.00
324_R	327_E	1.870	1.00
98_F	109_I	1.866	1.00
33_M	73_G	1.859	1.00
71_A	79_V	1.846	1.00
62_A	97_Q	1.838	1.00
48_W	80_G	1.825	1.00
184_G	211_S	1.818	1.00
287_I	294_V	1.808	1.00
243_T	247_Q	1.798	1.00
145_R	167_D	1.702	1.00
36_A	47_Y	1.674	1.00
38_R	42_W	1.638	1.00
142_E	146_N	1.630	1.00
207_L	210_N	1.630	1.00
211_S	214_P	1.626	1.00
98_F	111_L	1.625	1.00
70_I	79_V	1.605	1.00
152_G	155_R	1.604	1.00
38_R	323_M	1.599	1.00
293_E	296_A	1.593	1.00
174_G	190_F	1.576	1.00
233_D	324_R	1.560	1.00
324_R	328_I	1.551	1.00
145_R	186_L	1.538	1.00
181_R	214_P	1.538	1.00
203_P	206_E	1.531	1.00
231_A	294_V	1.527	1.00
292_E	296_A	1.508	1.00
24_T	27_E	1.445	1.00
33_M	69_H	1.443	1.00
295_K	299_E	1.439	1.00
181_R	185_E	1.433	1.00
237_K	241_L	1.425	1.00
299_E	302_V	1.413	1.00
152_G	157_Q	1.400	1.00
41_E	75_K	1.397	1.00
69_H	72_G	1.396	1.00
40_E	74_T	1.389	1.00
293_E	297_K	1.388	1.00
138_P	186_L	1.388	1.00
325_S	329_I	1.384	1.00
145_R	170_I	1.384	1.00
36_A	74_T	1.381	1.00
34_D	323_M	1.366	1.00
225_V	307_A	1.358	1.00
212_F	217_V	1.352	1.00
69_H	104_L	1.340	1.00
49_L	67_I	1.327	1.00
296_A	300_D	1.301	1.00
89_H	97_Q	1.300	1.00
232_A	238_A	1.286	1.00
125_R	128_R	1.281	1.00
291_P	328_I	1.278	1.00
319_H	323_M	1.270	1.00
27_E	31_N	1.269	1.00
160_A	163_G	1.263	1.00
30_R	34_D	1.257	1.00
62_A	65_V	1.256	1.00
222_Y	308_D	1.256	1.00
171_W	224_M	1.253	1.00
181_R	215_S	1.249	1.00
300_D	303_K	1.239	1.00
240_H	243_T	1.236	1.00
230_I	241_L	1.230	1.00
94_I	140_Q	1.230	1.00
237_K	240_H	1.222	1.00
34_D	38_R	1.221	1.00
231_A	291_P	1.220	1.00
177_G	207_L	1.215	1.00
138_P	142_E	1.208	1.00
40_E	75_K	1.207	1.00
185_E	215_S	1.205	1.00
171_W	189_P	1.201	1.00
184_G	215_S	1.199	1.00
259_Q	263_P	1.192	1.00
67_I	81_S	1.186	1.00
289_G	294_V	1.178	1.00
145_R	188_L	1.178	1.00
132_N	135_E	1.172	1.00
211_S	215_S	1.171	1.00
111_L	144_L	1.168	1.00
150_P	153_N	1.165	1.00
181_R	207_L	1.165	1.00
231_A	328_I	1.160	1.00
240_H	244_S	1.156	1.00
80_G	110_D	1.155	1.00
42_W	326_F	1.154	1.00
141_L	186_L	1.153	1.00
36_A	77_I	1.149	1.00
65_V	68_G	1.137	1.00
178_F	181_R	1.135	1.00
61_S	100_T	1.131	1.00
242_A	246_Y	1.127	1.00
198_P	300_D	1.125	1.00
233_D	237_K	1.122	1.00
113_L	141_L	1.121	1.00
143_E	155_R	1.113	1.00
49_L	70_I	1.112	1.00
144_L	148_F	1.110	1.00
101_L	109_I	1.103	1.00
164_E	167_D	1.095	1.00
64_A	97_Q	1.085	1.00
153_N	157_Q	1.078	1.00
135_E	178_F	1.077	1.00
202_V	305_T	1.077	1.00
119_T	123_T	1.069	1.00
287_I	297_K	1.068	1.00
65_V	101_L	1.065	1.00
37_R	75_K	1.064	1.00
124_A	129_R	1.051	1.00
261_K	264_V	1.047	1.00
212_F	218_L	1.041	1.00
34_D	319_H	1.039	1.00
136_D	139_E	1.035	1.00
93_V	97_Q	1.035	1.00
31_N	34_D	1.034	1.00
69_H	73_G	1.034	1.00
197_S	200_N	1.033	1.00
203_P	207_L	1.031	1.00
222_Y	309_E	1.022	1.00
143_E	146_N	1.015	1.00
146_N	151_S	1.015	1.00
102_E	165_G	1.013	1.00
41_E	327_E	1.011	1.00
239_Q	243_T	1.007	1.00
200_N	203_P	1.003	1.00
242_A	288_V	1.000	1.00
325_S	328_I	0.996	1.00
146_N	150_P	0.991	1.00
83_G	173_L	0.984	1.00
102_E	166_I	0.984	1.00
135_E	151_S	0.982	1.00
229_I	312_V	0.980	1.00
26_A	30_R	0.973	1.00
23_G	31_N	0.971	1.00
227_V	287_I	0.970	1.00
183_A	190_F	0.970	1.00
139_E	143_E	0.968	1.00
277_A	280_N	0.960	1.00
182_L	186_L	0.959	1.00
46_R	80_G	0.958	1.00
259_Q	262_P	0.957	1.00
227_V	298_L	0.955	1.00
236_E	239_Q	0.945	1.00
64_A	84_I	0.944	1.00
145_R	149_K	0.937	1.00
140_Q	144_L	0.937	1.00
106_P	163_G	0.932	1.00
138_P	182_L	0.930	1.00
213_T	218_L	0.928	1.00
31_N	319_H	0.928	1.00
132_N	136_D	0.921	1.00
180_A	211_S	0.919	1.00
232_A	241_L	0.916	1.00
124_A	130_N	0.913	1.00
191_A	224_M	0.901	1.00
312_V	329_I	0.901	1.00
30_R	33_M	0.901	1.00
209_R	223_A	0.891	1.00
91_S	143_E	0.876	1.00
296_A	299_E	0.872	1.00
83_G	114_G	0.870	1.00
34_D	41_E	0.865	1.00
123_T	128_R	0.862	1.00
231_A	329_I	0.862	1.00
260_L	263_P	0.858	1.00
126_A	129_R	0.856	1.00
250_L	254_R	0.856	1.00
136_D	140_Q	0.846	1.00
198_P	202_V	0.845	1.00
67_I	71_A	0.844	1.00
102_E	167_D	0.844	1.00
123_T	126_A	0.843	1.00
46_R	309_E	0.839	1.00
29_F	104_L	0.837	1.00
172_L	183_A	0.837	1.00
241_L	244_S	0.836	1.00
150_P	155_R	0.831	1.00
244_S	247_Q	0.829	1.00
239_Q	242_A	0.829	1.00
142_E	155_R	0.821	1.00
71_A	109_I	0.812	1.00
68_G	72_G	0.811	1.00
250_L	253_V	0.803	1.00
239_Q	283_L	0.800	1.00
180_A	184_G	0.800	1.00
103_T	166_I	0.796	1.00
28_S	31_N	0.796	1.00
85_M	116_A	0.795	1.00
205_L	208_Y	0.790	1.00
265_E	274_Y	0.789	1.00
72_G	105_Y	0.787	1.00
233_D	328_I	0.787	1.00
124_A	127_L	0.787	1.00
212_F	215_S	0.786	1.00
148_F	168_V	0.785	1.00
134_G	178_F	0.785	1.00
208_Y	212_F	0.783	1.00
160_A	164_E	0.781	1.00
71_A	101_L	0.780	1.00
126_A	130_N	0.779	1.00
244_S	248_R	0.779	1.00
86_L	94_I	0.777	1.00
190_F	208_Y	0.777	1.00
86_L	140_Q	0.777	1.00
243_T	246_Y	0.772	1.00
234_T	238_A	0.772	1.00
229_I	329_I	0.769	1.00
278_M	282_Q	0.763	1.00
123_T	127_L	0.763	1.00
94_I	144_L	0.763	1.00
138_P	178_F	0.761	1.00
278_M	281_E	0.758	1.00
206_E	306_Q	0.755	1.00
323_M	327_E	0.754	1.00
29_F	69_H	0.752	1.00
33_M	70_I	0.752	1.00
158_V	161_I	0.751	1.00
131_I	178_F	0.750	1.00
124_A	128_R	0.746	1.00
209_R	213_T	0.739	1.00
180_A	190_F	0.739	1.00
78_R	169_P	0.731	1.00
146_N	149_K	0.730	1.00
67_I	109_I	0.729	1.00
181_R	210_N	0.727	1.00
67_I	101_L	0.727	1.00
98_F	148_F	0.725	1.00
172_L	179_S	0.725	1.00
248_R	251_D	0.724	1.00
199_A	202_V	0.723	1.00
110_D	171_W	0.720	1.00
102_E	164_E	0.720	1.00
99_G	158_V	0.715	1.00
276_K	279_V	0.715	1.00
212_F	219_D	0.713	1.00
83_G	94_I	0.711	1.00
258_N	261_K	0.711	1.00
127_L	130_N	0.707	1.00
172_L	187_G	0.706	1.00
142_E	185_E	0.705	1.00
91_S	140_Q	0.705	1.00
146_N	153_N	0.704	1.00
25_I	28_S	0.703	1.00
125_R	129_R	0.702	1.00
115_R	133_S	0.702	1.00
53_H	96_E	0.702	1.00
239_Q	288_V	0.700	1.00
89_H	93_V	0.698	1.00
48_W	82_G	0.697	1.00
172_L	182_L	0.696	1.00
153_N	156_N	0.695	1.00
177_G	211_S	0.695	1.00
224_M	309_E	0.694	1.00
246_Y	249_F	0.691	1.00
81_S	109_I	0.690	1.00
90_S	93_V	0.688	1.00
255_G	258_N	0.682	1.00
208_Y	221_P	0.682	1.00
233_D	291_P	0.680	1.00
51_E	89_H	0.678	1.00
177_G	181_R	0.678	1.00
212_F	220_E	0.675	1.00
31_N	35_L	0.675	1.00
80_G	171_W	0.673	1.00
115_R	137_F	0.673	1.00
209_R	308_D	0.671	1.00
247_Q	251_D	0.668	1.00
174_G	179_S	0.667	1.00
276_K	280_N	0.665	1.00
302_V	307_A	0.663	1.00
49_L	66_L	0.662	1.00
262_P	265_E	0.658	1.00
46_R	311_M	0.658	1.00
178_F	182_L	0.657	1.00
33_M	72_G	0.652	1.00
30_R	72_G	0.651	1.00
132_N	151_S	0.650	1.00
39_A	326_F	0.650	1.00
45_H	76_K	0.649	1.00
226_G	311_M	0.648	1.00
205_L	305_T	0.645	1.00
259_Q	264_V	0.645	1.00
40_E	77_I	0.641	1.00
64_A	98_F	0.640	1.00
147_Y	153_N	0.640	1.00
79_V	105_Y	0.639	1.00
92_L	147_Y	0.638	1.00
223_A	307_A	0.638	1.00
265_E	273_P	0.637	1.00
102_E	105_Y	0.636	1.00
121_Q	124_A	0.634	1.00
235_N	238_A	0.634	1.00
159_R	162_P	0.632	1.00
39_A	77_I	0.632	1.00
47_Y	70_I	0.631	1.00
103_T	163_G	0.630	1.00
248_R	252_L	0.629	1.00
298_L	310_I	0.629	1.00
45_H	78_R	0.628	1.00
68_G	101_L	0.627	1.00
33_M	36_A	0.626	1.00
142_E	145_R	0.624	1.00
298_L	312_V	0.623	1.00
74_T	108_R	0.618	1.00
30_R	75_K	0.616	1.00
139_E	142_E	0.616	1.00
232_A	235_N	0.610	1.00
68_G	105_Y	0.609	1.00
251_D	254_R	0.609	1.00
169_P	222_Y	0.607	1.00
29_F	33_M	0.607	1.00
292_E	295_K	0.606	1.00
185_E	211_S	0.605	1.00
161_I	164_E	0.604	1.00
201_T	204_A	0.603	1.00
261_K	265_E	0.600	1.00
89_H	94_I	0.596	1.00
137_F	140_Q	0.595	1.00
229_I	287_I	0.594	1.00
192_F	204_A	0.594	1.00
146_N	155_R	0.592	1.00
113_L	144_L	0.591	1.00
42_W	323_M	0.589	1.00
184_G	212_F	0.589	1.00
66_L	100_T	0.588	1.00
135_E	152_G	0.586	1.00
314_S	326_F	0.585	1.00
86_L	113_L	0.584	1.00
122_L	125_R	0.582	1.00
215_S	218_L	0.582	1.00
314_S	329_I	0.582	1.00
185_E	214_P	0.581	1.00
291_P	295_K	0.581	1.00
102_E	162_P	0.577	1.00
27_E	30_R	0.576	1.00
200_N	204_A	0.574	1.00
275_E	279_V	0.573	1.00
209_R	212_F	0.573	1.00
143_E	153_N	0.572	1.00
42_W	327_E	0.567	1.00
209_R	219_D	0.567	1.00
121_Q	125_R	0.567	1.00
234_T	240_H	0.567	1.00
139_E	157_Q	0.566	1.00
254_R	275_E	0.563	1.00
280_N	284_S	0.563	1.00
112_G	173_L	0.563	1.00
143_E	147_Y	0.562	1.00
119_T	122_L	0.561	1.00
99_G	160_A	0.559	1.00
150_P	154_V	0.557	1.00
180_A	208_Y	0.555	1.00
23_G	27_E	0.553	1.00
285_S	297_K	0.553	1.00
159_R	164_E	0.550	0.99
132_N	152_G	0.548	0.99
279_V	283_L	0.548	0.99
209_R	306_Q	0.546	0.99
209_R	218_L	0.545	0.99
99_G	148_F	0.543	0.99
74_T	77_I	0.543	0.99
99_G	103_T	0.542	0.99
125_R	130_N	0.541	0.99
279_V	282_Q	0.541	0.99
210_N	213_T	0.538	0.99
35_L	326_F	0.538	0.99
113_L	170_I	0.536	0.99
220_E	308_D	0.534	0.99
218_L	221_P	0.533	0.99
297_K	301_F	0.532	0.99
84_I	94_I	0.530	0.99
122_L	130_N	0.529	0.99
227_V	310_I	0.526	0.99
102_E	168_V	0.525	0.99
175_S	194_A	0.525	0.99
154_V	157_Q	0.524	0.99
122_L	127_L	0.523	0.99
206_E	209_R	0.522	0.99
68_G	71_A	0.522	0.99
209_R	220_E	0.522	0.99
91_S	144_L	0.522	0.99
110_D	309_E	0.521	0.99
230_I	242_A	0.520	0.99
141_L	172_L	0.519	0.99
257_P	261_K	0.518	0.99
69_H	105_Y	0.518	0.99
245_H	248_R	0.518	0.99
122_L	126_A	0.518	0.99
32_S	35_L	0.517	0.99
208_Y	223_A	0.517	0.99
65_V	69_H	0.517	0.99
281_E	284_S	0.516	0.99
238_A	242_A	0.515	0.99
95_A	148_F	0.515	0.99
105_Y	109_I	0.513	0.99
137_F	172_L	0.511	0.99
65_V	104_L	0.511	0.99
204_A	207_L	0.511	0.99
175_S	204_A	0.509	0.99
63_T	81_S	0.507	0.99
171_W	309_E	0.506	0.99
171_W	222_Y	0.506	0.99
65_V	97_Q	0.502	0.99
228_T	313_N	0.500	0.99
177_G	203_P	0.500	0.99
182_L	185_E	0.500	0.99

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
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HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1lucA	1	0.9226	100	0.229	Contact Map
2wgkA	2	0.9702	100	0.242	Contact Map
4us5A	3	0.9583	100	0.248	Contact Map
1lucB	1	0.9196	100	0.248	Contact Map
2i7gA	2	0.9464	100	0.258	Contact Map
1z69A	4	0.9167	100	0.27	Contact Map
3raoA	2	0.9524	100	0.274	Contact Map
1ezwA	3	0.9256	100	0.285	Contact Map
3c8nA	2	0.9405	100	0.291	Contact Map
3b9oA	2	0.9583	100	0.292	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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