GREMLIN Database
EST - Carboxylesterase
UniProt: O32232 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 246 (219)
Sequences: 59713 (49235)
Seq/√Len: 3327.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
88_A110_G4.5501.00
51_E71_N3.7861.00
34_L234_H3.1831.00
73_M102_L2.9501.00
215_K236_D2.9451.00
217_Y236_D2.8081.00
190_R218_E2.8021.00
11_F44_T2.7301.00
115_C192_D2.6601.00
41_R238_Y2.5461.00
88_A109_K2.4811.00
86_S109_K2.4351.00
228_K232_L2.4231.00
114_M233_V2.3391.00
204_Y212_K2.3311.00
190_R216_W2.2201.00
102_L106_V2.0721.00
73_M106_V2.0411.00
37_Y234_H1.9821.00
89_A99_S1.9731.00
73_M77_E1.9071.00
197_T216_W1.8971.00
101_K105_T1.8791.00
76_Y87_I1.8621.00
96_G116_A1.8381.00
88_A241_L1.7791.00
17_A44_T1.7761.00
16_K88_A1.7711.00
103_G111_I1.7371.00
21_L50_Y1.7141.00
184_T207_V1.6821.00
17_A46_H1.6821.00
220_S228_K1.6801.00
21_L72_V1.6611.00
185_F215_K1.6241.00
202_I206_E1.6021.00
72_V102_L1.5911.00
50_Y98_F1.5831.00
22_H50_Y1.5791.00
22_H53_H1.5701.00
16_K109_K1.5671.00
236_D239_E1.5651.00
69_W101_K1.5651.00
88_A112_V1.5331.00
43_Y88_A1.5291.00
48_P72_V1.5171.00
28_T55_V1.5001.00
37_Y238_Y1.4721.00
113_P116_A1.4591.00
185_F240_F1.4591.00
99_S111_I1.4501.00
26_G30_D1.4471.00
232_L236_D1.4461.00
17_A81_S1.4331.00
70_K105_T1.4211.00
188_Q200_A1.4121.00
28_T49_Q1.3501.00
101_K104_Y1.3341.00
66_E105_T1.3331.00
35_G47_A1.3311.00
176_N179_M1.3251.00
92_L225_T1.3251.00
100_L113_P1.3181.00
25_T53_H1.2941.00
102_L108_I1.2851.00
46_H75_G1.2841.00
20_L90_C1.2451.00
235_Q239_E1.2441.00
28_T47_A1.2431.00
104_Y176_N1.2421.00
209_T212_K1.2391.00
112_V237_V1.2321.00
75_G79_L1.2301.00
90_C114_M1.2281.00
37_Y40_E1.2191.00
232_L235_Q1.1971.00
64_G67_D1.1891.00
217_Y220_S1.1861.00
204_Y214_L1.1631.00
201_N205_N1.1591.00
18_V43_Y1.1581.00
16_K86_S1.1551.00
25_T93_S1.1451.00
156_E159_P1.1421.00
188_Q216_W1.1401.00
16_K43_Y1.1331.00
188_Q214_L1.1161.00
197_T201_N1.1151.00
21_L99_S1.1121.00
20_L45_C1.1061.00
12_K15_D1.1031.00
112_V185_F1.0971.00
187_V217_Y1.0871.00
23_G93_S1.0851.00
207_V212_K1.0761.00
213_Q236_D1.0711.00
73_M105_T1.0501.00
81_S87_I1.0501.00
110_G183_P1.0421.00
30_D225_T1.0331.00
127_Y130_V1.0321.00
87_I108_I1.0301.00
141_E144_S1.0161.00
160_M163_L1.0071.00
155_F159_P0.9941.00
178_D206_E0.9861.00
185_F213_Q0.9861.00
201_N214_L0.9821.00
20_L31_V0.9781.00
19_L76_Y0.9751.00
201_N216_W0.9751.00
159_P162_T0.9721.00
39_N45_C0.9701.00
37_Y231_D0.9661.00
233_V237_V0.9601.00
90_C237_V0.9591.00
166_L170_I0.9561.00
21_L89_A0.9561.00
91_G99_S0.9531.00
69_W98_F0.9521.00
114_M225_T0.9461.00
50_Y94_L0.9451.00
163_L167_Q0.9421.00
46_H79_L0.9411.00
48_P75_G0.9411.00
202_I205_N0.9401.00
162_T167_Q0.9311.00
93_S222_H0.9311.00
221_G227_D0.9281.00
49_Q54_G0.9271.00
188_Q197_T0.9251.00
217_Y232_L0.9251.00
160_M164_K0.9241.00
161_N164_K0.9221.00
92_L223_V0.9201.00
81_S86_S0.9171.00
166_L169_L0.9161.00
32_R47_A0.9131.00
215_K232_L0.8921.00
161_N165_A0.8911.00
220_S227_D0.8911.00
76_Y102_L0.8861.00
147_Q151_E0.8781.00
77_E106_V0.8741.00
101_K176_N0.8731.00
21_L48_P0.8571.00
128_Q132_S0.8561.00
231_D235_Q0.8541.00
19_L87_I0.8541.00
201_N204_Y0.8471.00
37_Y41_R0.8401.00
163_L166_L0.8381.00
190_R219_E0.8351.00
164_K168_D0.8331.00
162_T166_L0.8331.00
43_Y241_L0.8331.00
144_S147_Q0.8291.00
146_E149_E0.8291.00
68_W94_L0.8231.00
24_F94_L0.8221.00
145_P149_E0.8201.00
33_M36_R0.8201.00
215_K239_E0.8161.00
156_E160_M0.8141.00
17_A87_I0.8141.00
186_V212_K0.8111.00
76_Y81_S0.8111.00
156_E163_L0.8101.00
151_E154_E0.8071.00
165_A168_D0.8071.00
66_E101_K0.8061.00
74_D78_Y0.7991.00
114_M224_I0.7941.00
69_W105_T0.7911.00
142_G145_P0.7881.00
104_Y179_M0.7871.00
73_M76_Y0.7861.00
28_T48_P0.7841.00
35_G45_C0.7841.00
92_L115_C0.7811.00
18_V90_C0.7801.00
155_F158_T0.7781.00
220_S224_I0.7781.00
187_V236_D0.7771.00
128_Q131_L0.7741.00
97_V117_P0.7731.00
115_C224_I0.7711.00
36_R39_N0.7671.00
89_A108_I0.7661.00
187_V233_V0.7591.00
90_C112_V0.7581.00
111_I184_T0.7581.00
15_D44_T0.7571.00
74_D77_E0.7521.00
50_Y68_W0.7511.00
144_S151_E0.7481.00
190_R197_T0.7461.00
11_F15_D0.7421.00
70_K73_M0.7401.00
49_Q55_V0.7361.00
18_V45_C0.7351.00
200_A214_L0.7341.00
158_T166_L0.7251.00
162_T165_A0.7231.00
62_H67_D0.7201.00
169_L172_D0.7191.00
87_I109_K0.7181.00
208_E212_K0.7171.00
234_H238_Y0.7151.00
110_G241_L0.7141.00
147_Q152_M0.7141.00
67_D70_K0.7131.00
16_K110_G0.7071.00
103_G108_I0.7061.00
22_H48_P0.7031.00
35_G39_N0.7021.00
159_P163_L0.6951.00
91_G96_G0.6931.00
36_R40_E0.6891.00
167_Q170_I0.6881.00
230_R234_H0.6831.00
148_I152_M0.6831.00
129_G133_Y0.6821.00
199_S202_I0.6811.00
103_G182_S0.6771.00
158_T162_T0.6761.00
147_Q150_E0.6751.00
126_M129_G0.6751.00
19_L89_A0.6711.00
141_E145_P0.6681.00
150_E153_K0.6661.00
149_E152_M0.6621.00
115_C189_A0.6591.00
165_A169_L0.6591.00
43_Y238_Y0.6591.00
112_V241_L0.6581.00
50_Y53_H0.6581.00
17_A43_Y0.6571.00
158_T163_L0.6551.00
115_C188_Q0.6501.00
191_H219_E0.6501.00
70_K74_D0.6481.00
164_K167_Q0.6461.00
235_Q238_Y0.6441.00
127_Y131_L0.6431.00
80_K85_E0.6421.00
158_T161_N0.6421.00
148_I151_E0.6401.00
173_V176_N0.6371.00
19_L72_V0.6371.00
32_R36_R0.6341.00
40_E238_Y0.6311.00
143_K147_Q0.6301.00
159_P169_L0.6271.00
100_L118_M0.6251.00
217_Y228_K0.6251.00
110_G240_F0.6231.00
144_S152_M0.6191.00
146_E150_E0.6181.00
29_A32_R0.6151.00
89_A102_L0.6141.00
14_G44_T0.6111.00
23_G92_L0.6091.00
18_V241_L0.6091.00
162_T170_I0.6091.00
46_H81_S0.6041.00
13_G44_T0.6031.00
157_K161_N0.5951.00
92_L114_M0.5951.00
159_P170_I0.5941.00
75_G78_Y0.5941.00
162_T169_L0.5931.00
219_E228_K0.5921.00
159_P167_Q0.5921.00
129_G132_S0.5911.00
130_V133_Y0.5901.00
69_W102_L0.5901.00
228_K231_D0.5891.00
157_K160_M0.5841.00
145_P151_E0.5811.00
18_V88_A0.5771.00
144_S149_E0.5751.00
145_P148_I0.5741.00
33_M230_R0.5721.00
113_P186_V0.5681.00
142_G151_E0.5661.00
38_L237_V0.5641.00
24_F53_H0.5631.00
198_E201_N0.5621.00
131_L134_A0.5591.00
130_V134_A0.5571.00
174_R206_E0.5561.00
51_E68_W0.5551.00
149_E153_K0.5551.00
103_G180_I0.5551.00
11_F14_G0.5541.00
111_I182_S0.5541.00
67_D71_N0.5531.00
143_K146_E0.5521.00
163_L170_I0.5451.00
31_V35_G0.5451.00
34_L37_Y0.5441.00
159_P164_K0.5391.00
142_G147_Q0.5371.00
19_L48_P0.5371.00
25_T94_L0.5361.00
91_G95_G0.5361.00
30_D92_L0.5351.00
142_G152_M0.5351.00
186_V214_L0.5341.00
81_S85_E0.5341.00
108_I182_S0.5341.00
34_L233_V0.5261.00
69_W73_M0.5191.00
126_M130_V0.5161.00
20_L47_A0.5121.00
49_Q56_P0.5111.00
143_K151_E0.5111.00
15_D43_Y0.5101.00
223_V226_L0.5081.00
159_P166_L0.5071.00
105_T176_N0.5051.00
143_K148_I0.5031.00
156_E162_T0.5031.00
156_E164_K0.5031.00
26_G223_V0.5021.00
127_Y134_A0.5001.00
149_E154_E0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3oosA 1 0.9472 100 0.247 Contact Map
3om8A 4 0.9472 100 0.25 Contact Map
4inzA 1 0.9512 100 0.251 Contact Map
2pujA 4 0.9675 100 0.252 Contact Map
2xuaA 2 0.9512 100 0.253 Contact Map
1ehyA 1 0.9268 100 0.255 Contact Map
1womA 1 0.9634 100 0.255 Contact Map
3bf7A 1 0.9512 100 0.257 Contact Map
4psuA 1 0.9512 100 0.258 Contact Map
1iupA 2 0.9634 100 0.263 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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