GREMLIN Database
AZOR2 - FMN-dependent NADH-azoreductase 2
UniProt: O32224 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 211 (187)
Sequences: 4933 (3515)
Seq/√Len: 257.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
3_K38_E3.8631.00
40_V92_S3.4241.00
25_A201_I2.9551.00
40_V89_Q2.9261.00
42_I89_Q2.8681.00
51_H109_P2.7661.00
126_K133_V2.7631.00
3_K40_V2.7121.00
91_A123_K2.5901.00
26_F144_I2.4181.00
24_K28_E2.3721.00
88_D92_S2.2631.00
43_D46_K2.1451.00
141_A170_M2.1361.00
51_H113_A2.1171.00
4_V37_D2.0351.00
3_K92_S1.9901.00
6_Y27_I1.9801.00
143_H180_G1.9201.00
190_P194_Q1.8281.00
9_A110_V1.8211.00
84_G88_D1.8061.00
141_A175_V1.7971.00
95_K142_I1.7661.00
87_S123_K1.7641.00
119_A173_F1.7521.00
115_L169_M1.7431.00
8_T23_G1.7111.00
19_S146_A1.6821.00
29_S205_K1.6041.00
8_T41_H1.5771.00
107_F165_Y1.5751.00
23_G99_V1.5621.00
198_E201_I1.5471.00
115_L119_A1.5261.00
50_P53_D1.5161.00
11_P103_W1.5111.00
27_I31_K1.5021.00
25_A29_S1.5021.00
26_F30_Y1.4971.00
96_Y141_A1.4871.00
194_Q198_E1.4591.00
88_D91_A1.4571.00
8_T20_M1.4171.00
82_R85_E1.4171.00
25_A28_E1.4061.00
95_K140_K1.3731.00
140_K179_D1.3531.00
30_Y37_D1.3511.00
88_D123_K1.3211.00
45_Y110_V1.3211.00
90_F118_V1.3201.00
193_A197_K1.3161.00
87_S124_S1.3121.00
4_V30_Y1.3011.00
96_Y175_V1.2691.00
28_E32_E1.2621.00
28_E31_K1.2451.00
29_S32_E1.2431.00
6_Y24_K1.2321.00
160_E164_R1.2311.00
128_T131_G1.2241.00
119_A169_M1.2211.00
90_F96_Y1.1911.00
25_A205_K1.1811.00
4_V95_K1.1801.00
29_S33_A1.1791.00
18_Y186_H1.1651.00
19_S99_V1.1601.00
198_E202_A1.1441.00
126_K135_L1.1421.00
105_F172_F1.1421.00
6_Y23_G1.1361.00
47_E86_L1.1311.00
112_K116_D1.1271.00
199_D203_R1.1261.00
22_T204_A1.1241.00
53_D56_V1.1221.00
23_G41_H1.1191.00
202_A205_K1.1171.00
9_A111_M1.1111.00
84_G123_K1.0981.00
189_E192_K1.0931.00
140_K177_S1.0901.00
91_A136_L1.0871.00
136_L175_V1.0831.00
22_T146_A1.0831.00
11_P110_V1.0801.00
118_V173_F1.0711.00
81_G85_E1.0641.00
57_F61_G1.0591.00
47_E85_E1.0541.00
163_H167_G1.0431.00
93_A96_Y1.0311.00
30_Y95_K1.0231.00
128_T133_V1.0221.00
18_Y193_A1.0121.00
37_D95_K1.0121.00
6_Y41_H1.0121.00
5_L89_Q1.0121.00
7_I98_F0.9961.00
192_K195_Q0.9941.00
26_F142_I0.9921.00
83_L120_V0.9861.00
100_T111_M0.9841.00
13_D46_K0.9671.00
89_Q114_Y0.9591.00
147_R182_F0.9591.00
21_A201_I0.9441.00
113_A117_S0.9401.00
115_L165_Y0.9351.00
42_I86_L0.9331.00
49_I117_S0.9321.00
51_H117_S0.9301.00
150_Y185_G0.9241.00
98_F111_M0.9181.00
86_L114_Y0.9121.00
21_A193_A0.9071.00
191_D195_Q0.9061.00
104_N116_D0.9051.00
4_V97_V0.8961.00
109_P113_A0.8961.00
201_I205_K0.8911.00
24_K41_H0.8891.00
42_I114_Y0.8881.00
147_R184_E0.8841.00
18_Y197_K0.8751.00
145_Q182_F0.8731.00
100_T143_H0.8711.00
31_K38_E0.8691.00
94_D139_K0.8671.00
49_I113_A0.8631.00
22_T144_I0.8621.00
27_I41_H0.8570.99
127_Y132_P0.8510.99
147_R162_G0.8490.99
167_G171_N0.8480.99
5_L93_A0.8460.99
195_Q199_D0.8440.99
133_V137_T0.8350.99
29_S201_I0.8310.99
150_Y184_E0.8250.99
161_M164_R0.8200.99
107_F111_M0.8150.99
87_S120_V0.8080.99
33_A37_D0.8060.99
142_I181_I0.8000.99
84_G120_V0.7900.99
157_A160_E0.7830.99
201_I204_A0.7820.99
90_F114_Y0.7760.99
138_D177_S0.7740.99
40_V93_A0.7700.99
151_Y159_M0.7680.99
56_V117_S0.7660.99
102_L162_G0.7630.99
38_E92_S0.7620.99
13_D54_A0.7530.99
155_P160_E0.7520.99
7_I44_L0.7500.99
190_P193_A0.7490.99
28_E201_I0.7470.99
98_F170_M0.7440.99
161_M168_I0.7420.99
18_Y183_V0.7420.99
8_T24_K0.7410.99
82_R117_S0.7410.99
150_Y153_E0.7390.99
26_F97_V0.7350.98
7_I42_I0.7300.98
21_A197_K0.7290.98
56_V60_W0.7250.98
59_G80_V0.7230.98
151_Y154_G0.7210.98
90_F136_L0.7190.98
22_T183_V0.7190.98
103_W108_P0.7190.98
83_L87_S0.7180.98
160_E163_H0.7140.98
102_L147_R0.7120.98
146_A186_H0.7100.98
51_H110_V0.7090.98
198_E205_K0.7060.98
12_H58_S0.6990.98
144_I200_A0.6990.98
83_L117_S0.6970.98
20_M23_G0.6950.98
18_Y21_A0.6920.98
195_Q198_E0.6870.98
78_A81_G0.6850.98
101_P108_P0.6840.98
144_I181_I0.6820.97
197_K200_A0.6790.97
115_L166_I0.6790.97
52_I56_V0.6750.97
100_T162_G0.6740.97
155_P159_M0.6730.97
147_R154_G0.6710.97
59_G62_K0.6700.97
82_R86_L0.6630.97
9_A45_Y0.6600.97
44_L110_V0.6570.97
85_E88_D0.6450.96
140_K175_V0.6430.96
192_K196_I0.6420.96
164_R168_I0.6360.96
3_K94_D0.6360.96
105_F168_I0.6350.96
56_V80_V0.6330.96
77_K81_G0.6330.96
126_K129_E0.6310.96
3_K93_A0.6290.96
85_E89_Q0.6290.96
87_S91_A0.6250.96
191_D194_Q0.6230.96
151_Y184_E0.6190.96
25_A197_K0.6180.96
6_Y99_V0.6130.95
49_I82_R0.6130.95
47_E89_Q0.6120.95
103_W110_V0.6100.95
188_A192_K0.6090.95
167_G180_G0.6060.95
196_I199_D0.6000.95
61_G64_Q0.5960.95
104_N125_F0.5960.95
130_Q133_V0.5940.94
109_P117_S0.5940.94
8_T43_D0.5920.94
26_F95_K0.5910.94
8_T99_V0.5900.94
156_A161_M0.5880.94
155_P158_E0.5850.94
193_A196_I0.5850.94
23_G27_I0.5850.94
57_F60_W0.5790.94
170_M178_F0.5770.94
106_S112_K0.5750.93
98_F166_I0.5740.93
199_D202_A0.5730.93
107_F169_M0.5720.93
145_Q162_G0.5720.93
21_A25_A0.5700.93
126_K130_Q0.5620.93
86_L89_Q0.5600.93
47_E82_R0.5580.92
132_P172_F0.5560.92
163_H180_G0.5550.92
153_E157_A0.5550.92
22_T200_A0.5520.92
194_Q197_K0.5520.92
158_E164_R0.5440.92
159_M168_I0.5410.91
143_H170_M0.5400.91
179_D203_R0.5390.91
53_D57_F0.5380.91
108_P113_A0.5370.91
87_S118_V0.5350.91
44_L86_L0.5350.91
53_D79_K0.5320.91
31_K41_H0.5290.90
138_D176_P0.5240.90
12_H54_A0.5240.90
58_S62_K0.5220.90
81_G84_G0.5210.90
129_E133_V0.5190.90
102_L151_Y0.5190.90
169_M173_F0.5160.89
100_T108_P0.5150.89
58_S61_G0.5120.89
27_I39_V0.5110.89
154_G157_A0.5100.89
106_S165_Y0.5090.89
171_N178_F0.5070.88
42_I47_E0.5050.88
165_Y169_M0.5040.88
143_H178_F0.5030.88
150_Y154_G0.5030.88
102_L145_Q0.5020.88
12_H17_S0.5000.88
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3w77A 2 0.981 100 0.348 Contact Map
3w7aA 2 0.9953 100 0.353 Contact Map
2hpvA 2 0.9621 100 0.368 Contact Map
1d4aA 2 0.981 100 0.371 Contact Map
3u7iA 2 0.9668 100 0.379 Contact Map
4n65A 2 0.9479 100 0.389 Contact Map
4gi5A 2 0.981 100 0.393 Contact Map
4c0xA 2 0.9384 100 0.393 Contact Map
1t5bA 2 0.9384 100 0.394 Contact Map
4fglA 2 0.981 100 0.417 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0077 seconds.