GREMLIN Database
BSN2 - Extracellular ribonuclease
UniProt: O32150 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 288 (229)
Sequences: 711 (491)
Seq/√Len: 32.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
246_L283_A4.9331.00
256_D260_K4.0521.00
247_E251_Q3.4941.00
68_A83_I2.9641.00
276_F286_I2.9511.00
141_K153_G2.9331.00
249_N283_A2.8301.00
108_N111_N2.7991.00
160_R208_M2.7671.00
67_G79_T2.7101.00
204_D252_D2.6051.00
196_E202_K2.5961.00
115_L205_V2.5621.00
177_N247_E2.5571.00
98_A102_T2.4410.99
60_D63_T2.4250.99
71_K79_T2.3780.99
156_I265_I2.3370.99
173_M190_Y2.2660.99
138_V205_V2.2370.99
102_T156_I2.2220.99
199_D202_K2.1890.99
250_K284_D2.1610.99
105_D111_N2.1390.99
81_H85_S2.1200.98
126_N164_V2.0850.98
68_A71_K2.0670.98
200_D255_D2.0650.98
209_L227_L2.0220.98
157_H208_M1.9460.97
214_V276_F1.9400.97
63_T66_E1.9060.97
131_G184_K1.8470.96
98_A156_I1.8050.96
256_D259_R1.7990.96
90_L98_A1.7980.96
246_L250_K1.7710.95
99_L159_L1.7580.95
123_K126_N1.7500.95
118_N198_R1.6940.94
69_E112_V1.6880.94
239_H243_S1.6820.94
132_D164_V1.6760.94
165_Q169_A1.6510.93
71_K75_S1.6130.92
101_E264_I1.6060.92
111_N120_S1.6000.92
60_D66_E1.5900.92
74_D235_S1.5870.92
131_G165_Q1.5850.92
81_H218_G1.5380.90
266_Y271_H1.5180.90
77_K214_V1.5110.89
259_R263_E1.4860.89
105_D113_I1.4750.88
91_S94_E1.4670.88
138_V208_M1.4630.88
101_E269_Y1.4610.88
188_N195_W1.4390.87
95_V159_L1.4270.87
139_W212_M1.3750.84
79_T82_R1.3610.84
143_H174_D1.3600.84
263_E267_E1.3560.84
123_K132_D1.3390.83
61_T66_E1.3390.83
153_G159_L1.3340.83
143_H209_L1.3330.82
170_R175_F1.3280.82
102_T269_Y1.3130.82
138_V160_R1.3050.81
156_I269_Y1.2920.80
185_A189_Y1.2680.79
144_G152_P1.2660.79
108_N113_I1.2580.79
77_K217_E1.2420.78
58_Y61_T1.2410.78
246_L249_N1.2260.77
105_D120_S1.2160.76
58_Y62_D1.2030.75
68_A79_T1.1970.75
279_H282_W1.1900.75
126_N132_D1.1860.74
141_K159_L1.1860.74
145_D153_G1.1830.74
75_S79_T1.1830.74
138_V209_L1.1730.74
118_N201_V1.1700.73
198_R201_V1.1550.72
115_L138_V1.1340.71
102_T265_I1.1160.70
196_E199_D1.1000.69
98_A269_Y1.0970.68
165_Q184_K1.0960.68
102_T160_R1.0910.68
113_I201_V1.0860.68
158_H196_E1.0790.67
128_G131_G1.0680.66
82_R85_S1.0670.66
144_G158_H1.0540.65
148_T153_G1.0540.65
169_A195_W1.0490.65
58_Y63_T1.0490.65
282_W285_E1.0430.64
210_F283_A1.0270.63
139_W240_G1.0210.63
228_N233_N1.0200.63
65_Y275_P1.0190.63
176_D194_S1.0090.62
99_L112_V1.0030.61
96_W135_R1.0010.61
264_I269_Y0.9990.61
76_L286_I0.9810.60
215_R253_P0.9780.59
123_K164_V0.9660.58
150_K154_T0.9640.58
182_Y195_W0.9480.57
157_H160_R0.9440.57
84_I215_R0.9430.56
98_A265_I0.9320.56
210_F214_V0.9310.56
144_G151_G0.9280.55
86_G215_R0.9150.54
126_N131_G0.9120.54
185_A188_N0.9110.54
158_H208_M0.9110.54
121_R125_L0.9050.53
98_A112_V0.9030.53
127_G164_V0.9030.53
229_D243_S0.8950.53
210_F242_Q0.8920.52
138_V141_K0.8910.52
87_H216_Y0.8850.52
220_D223_P0.8840.52
195_W210_F0.8810.51
95_V99_L0.8790.51
208_M265_I0.8780.51
62_D251_Q0.8770.51
224_D227_L0.8750.51
226_E229_D0.8720.51
74_D78_S0.8710.51
61_T83_I0.8650.50
191_D194_S0.8640.50
160_R205_V0.8550.49
115_L160_R0.8540.49
258_E277_I0.8530.49
119_E122_S0.8520.49
139_W144_G0.8510.49
245_L249_N0.8360.48
147_G151_G0.8330.47
200_D204_D0.8320.47
75_S247_E0.8230.47
141_K212_M0.8190.46
78_S282_W0.8160.46
60_D279_H0.8130.46
140_A239_H0.8120.46
144_G170_R0.8110.46
216_Y270_Q0.8100.46
125_L132_D0.8050.45
139_W143_H0.8050.45
99_L144_G0.8010.45
87_H283_A0.7980.45
124_N131_G0.7910.44
135_R154_T0.7910.44
263_E266_Y0.7890.44
206_A275_P0.7870.44
96_W112_V0.7860.44
61_T112_V0.7840.43
67_G183_A0.7830.43
103_D160_R0.7740.43
191_D225_L0.7680.42
148_T172_N0.7660.42
80_L286_I0.7650.42
95_V135_R0.7630.42
252_D255_D0.7620.42
150_K153_G0.7610.42
68_A80_L0.7580.41
78_S82_R0.7510.41
83_I86_G0.7490.41
134_N170_R0.7480.41
260_K264_I0.7480.41
216_Y272_N0.7420.40
119_E171_G0.7390.40
62_D282_W0.7360.40
164_V171_G0.7310.39
106_P120_S0.7300.39
113_I120_S0.7300.39
107_R111_N0.7260.39
66_E267_E0.7200.38
199_D254_V0.7140.38
213_A287_W0.7140.38
124_N164_V0.7120.38
95_V161_P0.7110.38
114_L121_R0.7090.37
103_D106_P0.7070.37
91_S154_T0.7070.37
104_E200_D0.7020.37
71_K251_Q0.6980.37
69_E79_T0.6960.36
210_F254_V0.6920.36
254_V258_E0.6920.36
284_D287_W0.6920.36
141_K205_V0.6860.36
225_L229_D0.6850.36
78_S217_E0.6850.36
123_K127_G0.6790.35
138_V257_R0.6780.35
126_N130_V0.6770.35
177_N244_V0.6750.35
159_L172_N0.6750.35
210_F287_W0.6740.35
89_M150_K0.6690.34
157_H277_I0.6680.34
123_K128_G0.6680.34
210_F245_L0.6670.34
60_D263_E0.6660.34
172_N235_S0.6640.34
154_T170_R0.6630.34
65_Y206_A0.6630.34
64_Y117_T0.6620.34
223_P226_E0.6610.34
67_G75_S0.6610.34
138_V175_F0.6610.34
125_L129_N0.6600.34
143_H223_P0.6580.34
150_K171_G0.6560.33
142_S172_N0.6540.33
210_F227_L0.6540.33
101_E104_E0.6490.33
139_W245_L0.6420.32
186_P190_Y0.6330.32
85_S254_V0.6330.32
258_E272_N0.6330.32
215_R272_N0.6310.32
67_G71_K0.6310.32
86_G94_E0.6300.32
186_P189_Y0.6290.32
148_T154_T0.6270.31
104_E108_N0.6270.31
240_G244_V0.6230.31
75_S250_K0.6230.31
264_I267_E0.6220.31
227_L246_L0.6190.31
133_W170_R0.6160.31
260_K263_E0.6150.31
140_A175_F0.6120.30
122_S128_G0.6110.30
70_G76_L0.6100.30
66_E75_S0.6100.30
141_K148_T0.6070.30
247_E250_K0.6060.30
94_E149_S0.6020.30
104_E141_K0.6010.30
106_P111_N0.6000.29
249_N284_D0.5990.29
214_V271_H0.5980.29
141_K158_H0.5970.29
148_T233_N0.5960.29
92_Y137_H0.5940.29
101_E171_G0.5940.29
114_L178_G0.5920.29
121_R163_D0.5910.29
245_L270_Q0.5890.29
112_V158_H0.5880.29
143_H160_R0.5870.29
105_D108_N0.5850.28
85_S89_M0.5840.28
161_P166_V0.5830.28
127_G132_D0.5830.28
125_L131_G0.5810.28
221_G236_A0.5810.28
78_S85_S0.5800.28
170_R190_Y0.5800.28
175_F209_L0.5790.28
205_V209_L0.5790.28
213_A217_E0.5780.28
183_A189_Y0.5740.28
195_W220_D0.5730.28
160_R175_F0.5730.28
225_L228_N0.5720.28
258_E287_W0.5710.28
72_T249_N0.5710.28
210_F282_W0.5670.27
209_L239_H0.5660.27
160_R277_I0.5630.27
173_M192_G0.5630.27
244_V277_I0.5630.27
90_L96_W0.5610.27
98_A135_R0.5600.27
67_G82_R0.5590.27
86_G269_Y0.5540.26
139_W201_V0.5520.26
99_L161_P0.5520.26
121_R131_G0.5480.26
74_D234_G0.5480.26
264_I271_H0.5470.26
266_Y272_N0.5470.26
144_G196_E0.5450.26
215_R258_E0.5450.26
163_D252_D0.5420.26
152_P158_H0.5410.26
158_H212_M0.5410.26
163_D204_D0.5400.26
82_R86_G0.5390.25
233_N281_E0.5350.25
257_R287_W0.5340.25
128_G243_S0.5330.25
133_W144_G0.5330.25
139_W227_L0.5330.25
137_H167_N0.5320.25
178_G194_S0.5320.25
237_P287_W0.5310.25
68_A282_W0.5290.25
75_S180_T0.5250.25
216_Y253_P0.5230.24
134_N162_A0.5230.24
62_D148_T0.5230.24
201_V252_D0.5220.24
78_S214_V0.5210.24
70_G147_G0.5210.24
185_A193_D0.5190.24
88_T247_E0.5190.24
80_L83_I0.5180.24
58_Y175_F0.5170.24
247_E255_D0.5170.24
123_K131_G0.5160.24
172_N219_D0.5130.24
85_S244_V0.5120.24
213_A237_P0.5110.24
82_R192_G0.5110.24
103_D277_I0.5110.24
204_D255_D0.5080.24
70_G73_G0.5080.24
114_L174_D0.5060.23
222_Y227_L0.5060.23
153_G243_S0.5050.23
112_V175_F0.5050.23
139_W198_R0.5050.23
163_D166_V0.5030.23
94_E98_A0.5030.23
121_R184_K0.5020.23
117_T197_P0.5010.23
227_L239_H0.5010.23
83_I238_Y0.5000.23
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2pu3A 1 0.5833 100 0.487 Contact Map
4un4B 1 0.7951 70.7 0.957 Contact Map
3m7kA 2 0.4271 31.1 0.966 Contact Map
4h9dA 3 0.3368 25.7 0.968 Contact Map
4ogeA 1 0.7674 15.4 0.971 Contact Map
4p6kA 2 0.0521 7.4 0.975 Contact Map
4p1wA 1 0.1042 6.4 0.976 Contact Map
2mfqB 1 0.0799 4.2 0.978 Contact Map
4ph1A 1 0.0868 2.6 0.98 Contact Map
3zbiC 1 0.0972 2.2 0.981 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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