GREMLIN Database
PUCK - Uric acid permease PucK
UniProt: O32140 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 430 (392)
Sequences: 4325 (2243)
Seq/√Len: 113.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
167_G198_A4.9541.00
171_F194_A4.9021.00
103_I283_G4.8501.00
147_L165_G3.8971.00
134_I407_G3.8861.00
215_W326_K3.7771.00
172_I191_G3.6221.00
52_F322_G3.5441.00
108_I238_L3.5291.00
197_A201_F3.4271.00
208_S212_E3.4011.00
96_P228_T3.1181.00
378_L410_T2.9951.00
175_L190_L2.9431.00
100_G229_F2.9281.00
97_A228_T2.8781.00
207_F210_V2.7291.00
164_L199_A2.7211.00
375_S411_A2.6011.00
55_G319_V2.5991.00
164_L204_K2.5631.00
367_G419_Q2.4641.00
167_G194_A2.4421.00
222_F284_L2.4421.00
213_A326_K2.3821.00
94_G97_A2.3761.00
14_G313_I2.3421.00
18_M317_I2.3331.00
57_A278_A2.3121.00
103_I280_L2.3081.00
164_L202_M2.2831.00
252_Y271_G2.2751.00
104_A234_V2.2621.00
169_T345_V2.2061.00
193_I197_A2.2011.00
71_G265_E2.1891.00
196_T205_V2.1671.00
11_M317_I2.1491.00
10_L313_I2.0911.00
197_A200_Y2.0611.00
122_F416_A2.0611.00
59_L315_G2.0341.00
59_L312_A2.0301.00
53_M285_F2.0261.00
171_F198_A2.0131.00
210_V330_L2.0121.00
110_V280_L2.0061.00
110_V273_R1.9871.00
305_M310_V1.9811.00
107_L111_L1.9681.00
171_F175_L1.9641.00
163_L167_G1.9321.00
62_L312_A1.9261.00
211_L214_S1.9191.00
167_G171_F1.9131.00
32_V86_M1.9001.00
413_A417_F1.8781.00
107_L280_L1.8681.00
106_G280_L1.8601.00
160_D204_K1.8561.00
112_A245_L1.8111.00
86_M98_I1.8091.00
198_A202_M1.7951.00
159_L163_L1.7941.00
256_A263_L1.7551.00
209_E333_V1.7381.00
213_A329_A1.7371.00
196_T333_V1.7331.00
146_N336_T1.7041.00
259_T262_R1.6921.00
208_S211_L1.6721.00
137_I351_I1.6661.00
124_P415_H1.6631.00
52_F319_V1.6631.00
113_A273_R1.6571.00
175_L179_F1.6231.00
102_I283_G1.6071.00
264_S267_D1.5971.00
316_I320_A1.5861.00
182_F186_I1.5851.00
18_M314_T1.5691.00
375_S410_T1.5671.00
163_L166_F1.5621.00
174_L178_F1.5511.00
210_V213_A1.5421.00
110_V277_L1.5371.00
110_V276_G1.5361.00
54_C75_V1.5131.00
267_D270_K1.5061.00
172_I342_A1.5011.00
97_A230_E1.4631.00
230_E233_A1.4381.00
371_I414_L1.4341.00
64_R71_G1.4221.00
166_F170_A1.4101.00
4_Q309_N1.4041.00
195_G338_V1.3851.00
378_L406_I1.3771.00
179_F183_I1.3671.00
89_I233_A1.3671.00
183_I190_L1.3611.00
198_A201_F1.3601.00
200_Y207_F1.3591.00
261_R267_D1.3471.00
93_Y230_E1.3461.00
221_L284_L1.3341.00
59_L62_L1.3241.00
221_L286_N1.3201.00
17_H296_N1.3201.00
169_T374_S1.3181.00
83_V87_I1.3171.00
126_V372_I1.3171.00
160_D202_M1.3141.00
223_Y286_N1.3071.00
209_E212_E1.3041.00
170_A174_L1.2940.99
59_L319_V1.2940.99
103_I227_P1.2900.99
409_L413_A1.2860.99
58_T75_V1.2840.99
300_V306_K1.2830.99
201_F204_K1.2820.99
111_L115_F1.2750.99
63_W312_A1.2740.99
148_A156_F1.2730.99
257_D360_S1.2690.99
56_A319_V1.2690.99
8_L12_M1.2610.99
179_F184_R1.2550.99
361_V368_N1.2460.99
397_S401_G1.2450.99
363_L372_I1.2410.99
222_F225_G1.2370.99
229_F234_V1.2350.99
21_M25_A1.2290.99
255_L300_V1.2210.99
93_Y233_A1.2200.99
251_V294_S1.2120.99
106_G279_I1.2120.99
194_A198_A1.2100.99
374_S410_T1.2050.99
62_L311_I1.1940.99
74_V255_L1.1940.99
326_K329_A1.1860.99
52_F285_F1.1800.99
186_I190_L1.1580.99
106_G276_G1.1580.99
90_G93_Y1.1540.99
66_R270_K1.1490.99
134_I379_G1.1350.99
126_V130_S1.1220.98
324_V328_A1.1190.98
103_I284_L1.1150.98
190_L194_A1.1120.98
163_L202_M1.1100.98
81_T244_S1.1100.98
79_T244_S1.1010.98
91_S94_G1.0990.98
174_L417_F1.0970.98
109_V244_S1.0920.98
77_G293_F1.0910.98
144_M381_G1.0870.98
35_A220_S1.0770.98
200_Y205_V1.0740.98
217_H325_P1.0600.98
155_E158_S1.0550.98
66_R71_G1.0510.98
376_V380_L1.0430.98
7_A11_M1.0420.98
100_G104_A1.0390.98
251_V297_V1.0370.97
247_E291_T1.0360.97
96_P225_G1.0330.97
396_A399_L1.0300.97
5_H307_S1.0280.97
105_A241_A1.0280.97
133_M376_V1.0260.97
248_S252_Y1.0200.97
64_R277_L1.0190.97
160_D163_L1.0180.97
89_I237_M1.0170.97
231_L234_V1.0140.97
85_P240_V1.0110.97
149_G157_G1.0110.97
167_G202_M0.9990.97
100_G227_P0.9980.97
161_N337_P0.9890.97
76_L292_A0.9890.97
149_G156_F0.9850.97
5_H10_L0.9830.97
320_A324_V0.9810.96
222_F227_P0.9800.96
20_A299_I0.9790.96
396_A400_A0.9760.96
56_A281_L0.9700.96
11_M15_L0.9680.96
252_Y275_E0.9680.96
370_L374_S0.9640.96
163_L198_A0.9580.96
386_P400_A0.9560.96
160_D164_L0.9540.96
282_G290_Y0.9520.96
5_H310_V0.9520.96
15_L317_I0.9480.96
56_A60_L0.9440.96
4_Q10_L0.9410.96
309_N312_A0.9360.95
199_A204_K0.9350.95
248_S271_G0.9340.95
59_L316_I0.9290.95
93_Y97_A0.9270.95
281_L285_F0.9260.95
75_V314_T0.9240.95
28_V80_F0.9180.95
199_A203_G0.9170.95
74_V292_A0.9140.95
206_D209_E0.9020.94
164_L198_A0.8980.94
89_I101_A0.8880.94
218_V323_L0.8840.94
61_Q72_L0.8780.94
11_M313_I0.8770.94
215_W324_V0.8710.93
101_A233_A0.8680.93
140_I377_S0.8670.93
17_H21_M0.8650.93
24_G295_Q0.8650.93
17_H20_A0.8650.93
232_P236_T0.8600.93
133_M355_I0.8540.93
214_S217_H0.8540.93
352_S369_L0.8510.92
153_S158_S0.8470.92
196_T207_F0.8460.92
158_S161_N0.8450.92
79_T291_T0.8380.92
103_I229_F0.8380.92
145_N156_F0.8370.92
196_T334_I0.8370.92
153_S161_N0.8360.92
24_G347_F0.8350.92
327_A330_L0.8320.92
171_F191_G0.8320.92
6_N9_Q0.8310.92
207_F333_V0.8270.91
132_V251_V0.8220.91
364_D368_N0.8220.91
220_S223_Y0.8210.91
312_A315_G0.8190.91
351_I376_V0.8160.91
123_F408_S0.8150.91
55_G315_G0.8140.91
109_V276_G0.8100.90
242_I246_V0.8100.90
259_T306_K0.8100.90
189_L193_I0.8070.90
262_R266_K0.8030.90
74_V252_Y0.8000.90
146_N347_F0.7960.90
30_L339_L0.7950.90
28_V78_C0.7920.89
117_G269_E0.7910.89
30_L33_G0.7900.89
53_M281_L0.7890.89
113_A269_E0.7880.89
13_L17_H0.7870.89
53_M287_A0.7870.89
81_T346_M0.7860.89
62_L308_V0.7790.89
138_S404_I0.7790.89
177_Y352_S0.7780.89
373_A377_S0.7770.89
345_V370_L0.7760.89
97_A233_A0.7740.88
169_T173_L0.7710.88
74_V299_I0.7700.88
371_I375_S0.7700.88
60_L64_R0.7700.88
249_T268_L0.7690.88
191_G194_A0.7680.88
248_S275_E0.7660.88
312_A316_I0.7610.88
161_N204_K0.7610.88
75_V311_I0.7600.87
367_G371_I0.7570.87
148_A157_G0.7560.87
166_F202_M0.7550.87
175_L183_I0.7550.87
161_N164_L0.7530.87
110_V114_G0.7530.87
145_N153_S0.7490.87
352_S372_I0.7490.87
297_V300_V0.7460.87
321_I327_A0.7460.87
348_G352_S0.7460.87
122_F412_I0.7450.86
277_L281_L0.7440.86
18_M321_I0.7440.86
244_S291_T0.7440.86
84_G87_I0.7400.86
93_Y228_T0.7400.86
101_A105_A0.7340.86
103_I107_L0.7330.86
116_F272_Y0.7310.85
303_S317_I0.7290.85
231_L235_V0.7290.85
23_A29_P0.7280.85
97_A101_A0.7260.85
53_M282_G0.7260.85
242_I254_A0.7250.85
199_A202_M0.7240.85
197_A320_A0.7240.85
210_V326_K0.7240.85
210_V333_V0.7220.85
109_V241_A0.7210.85
363_L368_N0.7200.85
378_L382_A0.7200.85
215_W219_P0.7200.85
170_A173_L0.7140.84
104_A229_F0.7130.84
50_D78_C0.7130.84
153_S156_F0.7120.84
146_N339_L0.7110.84
379_G407_G0.7100.84
175_L191_G0.7100.84
96_P99_Y0.7100.84
118_K122_F0.7100.84
192_L342_A0.7050.83
83_V242_I0.7050.83
130_S375_S0.7050.83
48_A328_A0.7040.83
279_I290_Y0.7040.83
73_P311_I0.7010.83
78_C82_A0.7010.83
60_L274_A0.7000.83
50_D288_F0.7000.83
79_T82_A0.6990.83
107_L110_V0.6990.83
205_V333_V0.6960.82
14_G314_T0.6960.82
11_M211_L0.6930.82
176_F370_L0.6930.82
21_M296_N0.6920.82
25_A54_C0.6920.82
374_S414_L0.6880.82
52_F323_L0.6880.82
248_S291_T0.6870.82
266_K270_K0.6860.82
76_L248_S0.6850.81
183_I187_A0.6850.81
156_F385_V0.6850.81
197_A211_L0.6820.81
49_I53_M0.6820.81
164_L337_P0.6810.81
382_A406_I0.6800.81
176_F302_L0.6800.81
255_L292_A0.6760.81
32_V192_L0.6760.81
95_V99_Y0.6760.81
317_I321_I0.6730.80
88_S92_T0.6720.80
147_L344_I0.6720.80
218_V221_L0.6700.80
237_M337_P0.6680.80
170_A193_I0.6660.80
88_S91_S0.6650.80
50_D54_C0.6640.79
117_G121_R0.6630.79
194_A197_A0.6630.79
190_L193_I0.6590.79
84_G88_S0.6580.79
258_I301_Q0.6570.79
185_S419_Q0.6560.79
174_L179_F0.6560.79
109_V245_L0.6550.79
316_I360_S0.6550.79
54_C74_V0.6530.78
126_V368_N0.6520.78
210_V329_A0.6520.78
25_A50_D0.6500.78
149_G162_V0.6500.78
36_I90_G0.6490.78
131_V169_T0.6490.78
145_N148_A0.6490.78
362_D368_N0.6480.78
27_L30_L0.6480.78
148_A162_V0.6480.78
72_L271_G0.6470.78
27_L31_I0.6440.77
27_L146_N0.6440.77
162_V166_F0.6430.77
280_L284_L0.6420.77
193_I330_L0.6400.77
171_F174_L0.6390.77
207_F330_L0.6390.77
7_A317_I0.6380.77
86_M102_I0.6360.76
294_S353_Y0.6320.76
180_K184_R0.6320.76
403_G407_G0.6320.76
238_L241_A0.6310.76
21_M75_V0.6310.76
49_I52_F0.6300.76
86_M237_M0.6300.76
123_F181_G0.6300.76
18_M236_T0.6270.75
192_L246_V0.6270.75
118_K123_F0.6270.75
90_G98_I0.6260.75
255_L297_V0.6260.75
371_I419_Q0.6250.75
301_Q356_K0.6240.75
73_P101_A0.6230.75
247_E275_E0.6210.75
99_Y103_I0.6210.75
143_A168_V0.6210.75
356_K363_L0.6190.75
59_L63_W0.6180.74
153_S157_G0.6160.74
126_V371_I0.6150.74
218_V322_G0.6130.74
363_L369_L0.6120.74
58_T315_G0.6120.74
358_L361_V0.6100.73
132_V135_I0.6090.73
174_L413_A0.6090.73
128_T325_P0.6090.73
379_G403_G0.6080.73
377_S380_L0.6080.73
244_S247_E0.6070.73
57_A274_A0.6070.73
25_A28_V0.6050.73
4_Q7_A0.6030.73
226_P284_L0.6020.72
17_H299_I0.6010.72
213_A325_P0.6000.72
90_G95_V0.6000.72
73_P259_T0.5990.72
202_M327_A0.5990.72
14_G132_V0.5960.72
329_A332_T0.5940.71
81_T135_I0.5910.71
179_F378_L0.5890.71
133_M358_L0.5890.71
260_N306_K0.5880.71
241_A279_I0.5870.71
78_C291_T0.5870.71
329_A333_V0.5870.71
124_P298_G0.5870.71
103_I226_P0.5860.70
188_I299_I0.5850.70
251_V292_A0.5850.70
348_G369_L0.5850.70
320_A399_L0.5830.70
216_L324_V0.5810.70
118_K265_E0.5810.70
279_I282_G0.5810.70
179_F182_F0.5800.70
192_L339_L0.5770.69
142_T146_N0.5750.69
322_G328_A0.5740.69
200_Y203_G0.5730.69
372_I376_V0.5730.69
130_S411_A0.5720.69
26_I331_T0.5720.69
254_A353_Y0.5710.69
146_N343_M0.5680.68
249_T253_F0.5680.68
94_G228_T0.5680.68
263_L267_D0.5680.68
138_S402_S0.5670.68
251_V353_Y0.5650.68
15_L148_A0.5640.68
275_E291_T0.5620.67
138_S403_G0.5610.67
252_Y263_L0.5610.67
158_S163_L0.5600.67
140_I373_A0.5600.67
200_Y417_F0.5590.67
60_L110_V0.5590.67
79_T282_G0.5590.67
140_I380_L0.5580.67
165_G338_V0.5580.67
55_G59_L0.5570.67
161_N165_G0.5570.67
195_G203_G0.5570.67
129_G354_G0.5570.67
127_V415_H0.5550.66
177_Y413_A0.5550.66
87_I218_V0.5550.66
148_A153_S0.5530.66
374_S380_L0.5530.66
271_G275_E0.5520.66
97_A100_G0.5510.66
143_A165_G0.5510.66
53_M278_A0.5490.66
149_G386_P0.5490.66
98_I101_A0.5490.66
295_Q347_F0.5480.65
356_K360_S0.5480.65
14_G63_W0.5480.65
222_F226_P0.5470.65
21_M356_K0.5470.65
248_S292_A0.5470.65
89_I100_G0.5460.65
339_L342_A0.5450.65
250_G379_G0.5450.65
177_Y350_V0.5440.65
180_K420_T0.5440.65
224_F227_P0.5440.65
223_Y227_P0.5430.65
133_M351_I0.5430.65
175_L194_A0.5420.65
353_Y356_K0.5420.65
358_L369_L0.5410.64
255_L331_T0.5400.64
160_D198_A0.5390.64
208_S213_A0.5390.64
76_L279_I0.5380.64
108_I162_V0.5370.64
25_A78_C0.5370.64
294_S297_V0.5370.64
56_A285_F0.5350.64
294_S405_V0.5350.64
236_T399_L0.5340.63
361_V369_L0.5340.63
25_A318_L0.5330.63
89_I98_I0.5330.63
218_V242_I0.5330.63
407_G411_A0.5320.63
308_V312_A0.5310.63
135_I293_F0.5310.63
164_L192_L0.5310.63
11_M320_A0.5300.63
18_M237_M0.5280.63
301_Q344_I0.5280.63
244_S252_Y0.5280.63
7_A10_L0.5270.62
31_I87_I0.5270.62
412_I416_A0.5260.62
140_I344_I0.5260.62
22_Y50_D0.5260.62
61_Q308_V0.5250.62
181_G184_R0.5250.62
378_L383_T0.5240.62
58_T291_T0.5230.62
219_P345_V0.5230.62
125_P305_M0.5230.62
81_T240_V0.5220.62
313_I317_I0.5220.62
101_A234_V0.5220.62
171_F190_L0.5190.61
193_I417_F0.5180.61
202_M206_D0.5180.61
78_C143_A0.5170.61
101_A238_L0.5160.61
209_E285_F0.5160.61
91_S97_A0.5150.61
197_A216_L0.5150.61
19_L371_I0.5130.60
277_L312_A0.5120.60
101_A109_V0.5120.60
108_I112_A0.5120.60
305_M339_L0.5120.60
63_W66_R0.5110.60
29_P33_G0.5110.60
301_Q407_G0.5100.60
242_I412_I0.5100.60
295_Q349_M0.5090.60
296_N299_I0.5090.60
165_G303_S0.5090.60
358_L363_L0.5080.60
89_I249_T0.5080.60
71_G87_I0.5080.60
82_A279_I0.5070.59
155_E204_K0.5070.59
275_E373_A0.5050.59
121_R416_A0.5050.59
13_L294_S0.5040.59
309_N313_I0.5030.59
351_I355_I0.5030.59
34_A191_G0.5020.59
356_K364_D0.5020.59
211_L409_L0.5020.59
253_F304_K0.5010.59
242_I395_A0.5010.59
201_F218_V0.5000.58
184_R363_L0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3qe7A 2 0.9163 100 0.396 Contact Map
2cfqA 1 0.9047 89.9 0.954 Contact Map
1pw4A 1 0.8977 89.5 0.954 Contact Map
3wdoA 1 0.886 81 0.959 Contact Map
4ikvA 1 0.9256 73.9 0.962 Contact Map
3o7qA 1 0.4047 73.7 0.962 Contact Map
4u4vA 1 0.8767 73.7 0.962 Contact Map
4m64A 1 0.8791 65.9 0.964 Contact Map
4xnjA 1 0.9093 61.6 0.965 Contact Map
4j05A 3 0.8535 54.2 0.966 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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