GREMLIN Database
PUCJ - Uric acid permease PucJ
UniProt: O32139 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 449 (394)
Sequences: 4328 (2239)
Seq/√Len: 112.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
174_L197_A5.0801.00
170_G201_A5.0161.00
101_I286_G4.9061.00
145_M168_S4.1301.00
132_L410_G3.9561.00
175_I194_G3.8491.00
218_F329_K3.7721.00
106_F241_L3.5201.00
200_A204_I3.5041.00
50_L325_G3.4941.00
211_S215_E3.3791.00
178_L193_I3.2121.00
94_H231_A3.1981.00
167_L202_A3.0911.00
381_L413_T3.0711.00
95_A231_A3.0201.00
98_G232_F2.8361.00
210_F213_V2.7531.00
167_L207_K2.7051.00
225_F287_L2.6971.00
53_G322_V2.6811.00
378_S414_A2.6041.00
170_G197_A2.5411.00
370_Y422_S2.4791.00
172_L348_V2.3681.00
216_A329_K2.3441.00
16_V320_I2.3331.00
12_S316_T2.3171.00
255_F274_G2.3111.00
101_I283_L2.2991.00
55_A281_A2.2961.00
167_L205_M2.2751.00
102_A237_I2.2491.00
196_A200_A2.2061.00
9_F320_I2.1761.00
8_V316_T2.1421.00
170_G174_L2.1341.00
69_G268_D2.1251.00
166_L170_G2.1121.00
51_T288_F2.0911.00
57_L315_V2.0841.00
120_L419_L2.0411.00
57_L318_G2.0401.00
200_A203_A2.0321.00
108_F276_R2.0291.00
213_V333_L2.0121.00
108_F283_L1.9851.00
111_A276_R1.9721.00
60_T315_V1.9681.00
105_V109_L1.9651.00
174_L201_A1.9631.00
308_V313_I1.9591.00
416_F420_F1.8851.00
174_L178_L1.8851.00
104_G283_L1.8751.00
110_F248_I1.8371.00
259_G266_L1.8351.00
162_L166_L1.8331.00
84_M96_I1.8191.00
163_E207_K1.8101.00
105_V283_L1.7991.00
214_T217_P1.7951.00
212_S336_A1.7941.00
30_V84_M1.7891.00
199_T336_A1.7671.00
50_L322_V1.7611.00
175_I345_A1.7581.00
262_C265_P1.7551.00
135_L354_I1.7451.00
178_L182_L1.7181.00
201_A205_M1.7091.00
100_I286_G1.7071.00
216_A332_A1.7021.00
177_V181_F1.6811.00
166_L169_V1.6731.00
204_I207_K1.6731.00
144_N339_A1.6561.00
319_C323_C1.6531.00
122_P418_N1.6511.00
211_S214_T1.6371.00
108_F279_G1.6131.00
115_G272_V1.5601.00
185_F189_L1.5561.00
270_D273_K1.5541.00
16_V317_A1.5541.00
172_L377_C1.5401.00
52_C73_M1.5251.00
213_V216_A1.4901.00
108_F280_I1.4761.00
267_T270_D1.4671.00
374_T417_L1.4611.00
199_T208_V1.4521.00
62_R69_G1.4511.00
224_P289_N1.4481.00
233_E236_P1.4451.00
378_S413_T1.4371.00
92_G95_A1.4311.00
212_S215_E1.4211.00
186_A193_I1.4191.00
264_R270_D1.4171.00
124_V375_I1.4131.00
198_G341_V1.4071.00
226_Y289_N1.3871.00
203_A210_F1.3831.00
201_A204_I1.3821.00
163_E205_M1.3691.00
95_A233_E1.3431.00
15_H299_N1.3431.00
85_I88_G1.3421.00
87_I236_P1.3361.00
34_L88_G1.3331.00
56_T73_M1.3301.00
381_L409_T1.3281.00
169_V173_V1.3271.00
61_L315_V1.3261.00
57_L60_T1.3261.00
182_L186_A1.3241.00
148_G160_G1.3171.00
303_L309_K1.3111.00
158_E161_S1.3040.99
260_K363_T1.2980.99
104_G282_I1.2980.99
101_I230_P1.2970.99
412_L416_F1.2860.99
400_R404_S1.2820.99
397_A400_R1.2800.99
197_A201_A1.2760.99
19_M23_A1.2710.99
81_V85_I1.2690.99
173_V177_V1.2620.99
182_L187_R1.2600.99
329_K332_A1.2320.99
91_Y233_E1.2300.99
57_L322_V1.2260.99
377_C413_T1.2240.99
6_F10_T1.2210.99
146_A159_Y1.2140.99
224_P287_L1.2020.99
364_A371_H1.2020.99
366_L375_I1.1970.99
50_L288_F1.1920.99
54_V322_V1.1840.99
258_I303_L1.1830.99
60_T314_V1.1680.99
189_L193_I1.1670.99
193_I197_A1.1660.99
232_F237_I1.1600.99
72_V258_I1.1600.99
254_V297_A1.1570.99
124_V128_T1.1560.99
75_G296_F1.1410.99
94_H228_G1.1380.99
104_G279_G1.1340.99
77_S247_I1.1300.99
132_L382_G1.1240.98
220_Q328_P1.1080.98
33_A223_K1.1020.98
142_V384_G1.0950.98
91_Y95_A1.0860.98
101_I287_L1.0850.98
107_I247_I1.0770.98
79_V247_I1.0720.98
147_G159_Y1.0630.98
109_L113_F1.0510.98
66_I280_I1.0460.98
74_L295_T1.0430.98
170_G205_M1.0400.97
18_A302_L1.0390.97
254_V300_A1.0280.97
399_I402_L1.0260.97
234_I237_I1.0250.97
379_I383_I1.0240.97
103_A244_G1.0220.97
250_E294_N1.0180.97
255_F278_E1.0170.97
111_A272_V1.0120.97
203_A208_V1.0120.97
83_P243_V1.0060.97
87_I240_M1.0020.97
166_L205_M1.0010.97
327_I331_A1.0010.97
163_E166_L1.0010.97
67_G273_K0.9980.97
399_I403_V0.9940.97
373_L377_C0.9910.97
285_G293_Y0.9910.97
251_S255_F0.9880.97
54_V284_I0.9870.97
62_R280_I0.9840.96
101_I232_F0.9840.96
98_G102_A0.9670.96
72_V295_T0.9590.96
159_Y388_A0.9590.96
312_N315_V0.9560.96
177_V420_F0.9520.96
143_K159_Y0.9500.96
73_M317_A0.9490.96
13_L320_I0.9450.96
9_F13_L0.9370.95
323_C327_I0.9360.95
26_V78_F0.9340.95
31_G36_V0.9330.95
225_F228_G0.9320.95
251_S274_G0.9220.95
138_V380_A0.9170.95
131_T379_I0.9130.95
15_H18_A0.9120.95
284_I288_F0.9110.95
57_L319_C0.9110.95
54_V58_L0.9110.95
183_K187_R0.8920.94
131_T358_V0.8860.94
354_I379_I0.8800.94
163_E167_L0.8740.93
209_S212_S0.8730.93
99_S236_P0.8720.93
315_V318_G0.8720.93
136_S407_T0.8640.93
99_S103_A0.8610.93
178_L186_A0.8580.93
59_Q70_L0.8530.92
355_A372_L0.8520.92
98_G230_P0.8510.92
202_A206_G0.8490.92
202_A207_K0.8430.92
58_L62_R0.8400.92
91_Y236_P0.8380.92
192_L196_A0.8370.92
217_P220_Q0.8370.92
15_H19_M0.8360.92
147_G160_G0.8350.92
101_I229_A0.8340.92
141_G171_V0.8320.92
330_I333_L0.8310.91
199_T210_F0.8300.91
172_L176_L0.8300.91
367_K371_H0.8280.91
148_G159_Y0.8280.91
218_F327_I0.8260.91
235_G239_T0.8260.91
9_F316_T0.8250.91
121_F411_S0.8220.91
199_T337_V0.8210.91
164_N340_A0.8200.91
53_G318_G0.8170.91
77_S294_N0.8160.91
225_F230_P0.8160.91
111_A279_G0.8160.91
141_G168_S0.8140.91
221_I326_L0.8110.90
107_I279_G0.8050.90
224_P228_G0.8050.90
28_L342_L0.8040.90
167_L201_A0.8040.90
200_A323_C0.8010.90
245_I249_V0.8010.90
72_V302_L0.8010.90
247_I294_N0.8000.90
72_V255_F0.7990.90
11_L15_H0.7990.90
51_T290_A0.7980.90
262_C309_K0.7970.90
51_T284_I0.7970.90
130_V254_V0.7960.90
86_A90_N0.7940.90
60_T311_R0.7940.90
178_L194_G0.7910.89
221_I224_P0.7890.89
28_L31_G0.7890.89
95_A236_P0.7860.89
280_I284_I0.7850.89
51_T285_G0.7850.89
251_S278_E0.7840.89
315_V319_C0.7820.89
252_T271_L0.7810.89
161_S164_N0.7800.89
73_M314_V0.7790.89
146_A160_G0.7720.88
202_A205_M0.7710.88
269_K273_K0.7680.88
144_N350_F0.7680.88
173_V176_L0.7670.88
370_Y374_T0.7670.88
81_V245_I0.7660.88
213_V336_A0.7640.88
179_N305_L0.7630.88
22_G350_F0.7600.87
105_V108_F0.7590.87
300_A303_L0.7590.87
26_V76_S0.7580.87
251_S294_N0.7570.87
324_L330_I0.7560.87
23_A52_C0.7510.87
22_G298_Q0.7510.87
21_A27_P0.7500.87
120_L415_I0.7500.87
205_M330_I0.7480.87
169_V205_M0.7480.87
71_P314_V0.7450.86
79_V349_M0.7430.86
223_K226_Y0.7420.86
306_T320_I0.7390.86
348_V373_L0.7390.86
210_F336_A0.7370.86
112_R115_G0.7370.86
377_C417_L0.7370.86
107_I244_G0.7360.86
174_L194_G0.7350.86
179_N373_L0.7340.85
146_A165_L0.7330.85
144_N342_L0.7300.85
48_D291_F0.7290.85
12_S317_A0.7290.85
174_L193_I0.7290.85
265_P269_K0.7260.85
128_T378_S0.7220.85
374_T422_S0.7200.84
355_A375_I0.7190.84
194_G197_A0.7170.84
19_M299_N0.7160.84
213_V329_K0.7150.84
376_A380_A0.7140.84
351_G355_A0.7140.84
89_S92_G0.7120.84
16_V324_L0.7110.84
76_S80_A0.7110.84
82_T85_I0.7110.84
282_I293_Y0.7080.83
101_I105_V0.7070.83
159_Y162_L0.7060.83
5_S9_F0.7040.83
234_I238_L0.7030.83
145_M347_V0.7030.83
164_N167_L0.7020.83
148_G158_E0.6960.82
229_A287_L0.6950.82
245_I257_A0.6930.82
381_L385_A0.6930.82
385_A409_T0.6910.82
74_L251_S0.6900.82
167_L340_A0.6900.82
48_D52_C0.6900.82
88_G96_I0.6890.82
23_A48_D0.6870.82
374_T378_S0.6870.82
195_I345_A0.6860.81
87_I99_S0.6840.81
107_I248_I0.6840.81
94_H97_Y0.6840.81
48_D76_S0.6840.81
58_L277_A0.6830.81
86_A89_S0.6820.81
19_M73_M0.6800.81
95_A99_S0.6740.80
188_T422_S0.6740.80
25_L144_N0.6730.80
175_I348_V0.6700.80
193_I196_A0.6680.80
415_I419_L0.6670.80
195_I249_V0.6660.79
258_I300_A0.6660.79
186_A190_S0.6650.79
77_S80_A0.6650.79
258_I295_T0.6640.79
221_I325_G0.6640.79
163_E201_A0.6630.79
366_L371_H0.6620.79
84_M100_I0.6570.79
297_A356_S0.6560.78
213_V332_A0.6560.78
5_S320_I0.6560.78
382_G406_G0.6560.78
382_G410_G0.6560.78
131_T361_L0.6560.78
218_F222_P0.6540.78
320_I324_L0.6530.78
102_A232_F0.6530.78
166_L201_A0.6530.78
319_C363_T0.6490.78
25_L28_L0.6490.78
15_H302_L0.6480.78
197_A204_I0.6480.78
93_I97_Y0.6440.77
191_V302_L0.6430.77
195_I342_L0.6420.77
50_L326_L0.6420.77
36_V327_I0.6420.77
178_L190_S0.6410.77
82_T86_A0.6390.77
130_V133_I0.6360.76
143_K146_A0.6350.76
52_C72_V0.6350.76
366_L372_L0.6320.76
180_R353_V0.6320.76
250_E278_E0.6310.76
124_V371_H0.6290.76
79_V133_I0.6280.75
241_L244_G0.6280.75
71_P262_C0.6280.75
247_I250_E0.6270.75
361_L364_A0.6260.75
25_L29_L0.6260.75
197_A200_A0.6240.75
304_Q359_K0.6230.75
398_P401_I0.6220.75
261_I304_Q0.6210.75
138_V383_I0.6200.74
84_M88_G0.6200.74
99_S237_I0.6200.74
182_L185_F0.6190.74
23_A26_V0.6180.74
116_K120_L0.6170.74
70_L274_G0.6160.74
165_L169_V0.6160.74
68_I270_D0.6150.74
9_F214_T0.6140.74
30_V195_I0.6130.74
174_L177_V0.6100.73
59_Q67_G0.6100.73
332_A335_S0.6090.73
124_V374_T0.6090.73
58_L108_F0.6080.73
13_L146_A0.6080.73
144_N346_T0.6070.73
116_K121_F0.6040.73
406_G410_G0.6040.73
266_L270_D0.6040.73
71_P99_S0.6020.72
180_R355_A0.6020.72
332_A336_A0.6010.72
55_A277_A0.6010.72
359_K363_T0.6000.72
244_G282_I0.5960.72
196_A333_L0.5940.71
227_F230_P0.5930.71
283_L286_G0.5920.71
51_T281_A0.5900.71
99_S241_L0.5880.71
254_V295_T0.5880.71
196_A420_F0.5880.71
308_V342_L0.5870.70
122_P301_G0.5870.70
140_T144_N0.5860.70
282_I285_G0.5860.70
204_I208_V0.5850.70
131_T175_I0.5840.70
200_A214_T0.5820.70
138_V376_A0.5820.70
74_L282_I0.5810.70
254_V356_S0.5810.70
240_M340_A0.5810.70
12_S130_V0.5800.70
19_M359_K0.5800.70
109_L169_V0.5800.70
221_I245_I0.5790.69
121_F184_G0.5790.69
13_L90_N0.5790.69
126_T328_P0.5790.69
278_E294_N0.5790.69
210_F333_L0.5780.69
299_N302_L0.5770.69
54_V288_F0.5760.69
283_L287_L0.5760.69
143_K149_E0.5750.69
199_T203_A0.5740.69
147_G165_L0.5730.69
97_Y101_I0.5730.69
203_A420_F0.5720.68
216_A328_P0.5690.68
27_P31_G0.5680.68
131_T354_I0.5680.68
298_Q350_F0.5670.68
184_G187_R0.5670.68
57_L61_L0.5650.68
274_G278_E0.5650.68
377_C383_I0.5640.67
351_G372_L0.5640.67
53_G57_L0.5630.67
257_A356_S0.5630.67
247_I255_F0.5630.67
223_K228_G0.5620.67
410_G414_A0.5620.67
136_S405_D0.5620.67
88_G91_Y0.5610.67
79_V294_N0.5610.67
311_R315_V0.5600.67
84_M240_M0.5600.67
11_L297_A0.5600.67
178_L181_F0.5600.67
175_I358_V0.5600.67
76_S294_N0.5570.66
380_A383_I0.5570.66
5_S8_V0.5550.66
129_V172_L0.5550.66
56_T294_N0.5540.66
122_P127_G0.5540.66
198_G206_G0.5540.66
23_A321_L0.5520.66
88_G93_I0.5520.66
66_I266_L0.5520.66
421_F424_R0.5510.66
87_I96_I0.5500.65
167_L195_I0.5490.65
252_T256_Y0.5480.65
106_F110_F0.5480.65
127_G357_G0.5470.65
136_S406_G0.5470.65
375_I379_I0.5460.65
161_S166_L0.5450.65
356_S359_K0.5440.65
365_D371_H0.5430.64
66_I130_V0.5400.64
106_F165_L0.5400.64
138_V347_V0.5400.64
205_M208_V0.5390.64
23_A76_S0.5380.64
83_P406_G0.5370.64
144_N168_S0.5370.64
83_P86_A0.5360.64
312_N316_T0.5330.63
59_Q311_R0.5330.63
359_K366_L0.5320.63
325_G331_A0.5320.63
177_V182_L0.5300.63
178_L197_A0.5300.63
95_A98_G0.5300.63
80_A282_I0.5290.62
87_I98_G0.5290.62
381_L386_S0.5280.62
76_S141_G0.5280.62
57_L310_T0.5280.62
226_Y230_P0.5280.62
133_I296_F0.5270.62
125_V418_N0.5270.62
128_T414_A0.5260.62
24_I334_A0.5250.62
26_V48_D0.5250.62
253_G382_G0.5250.62
109_L173_V0.5240.62
91_Y231_A0.5240.62
196_A425_D0.5240.62
74_L255_F0.5230.62
56_T318_G0.5230.62
36_V288_F0.5220.61
17_L374_T0.5220.61
370_Y417_L0.5210.61
77_S285_G0.5210.61
222_P348_V0.5210.61
186_A267_T0.5190.61
30_V68_I0.5190.61
239_T402_L0.5190.61
278_E376_A0.5190.61
245_I415_I0.5170.61
297_A300_A0.5170.61
297_A408_I0.5170.61
411_S414_A0.5170.61
251_S295_T0.5150.60
119_V419_L0.5140.60
148_G164_N0.5140.60
160_G164_N0.5140.60
280_I315_V0.5140.60
165_L386_S0.5130.60
256_Y259_G0.5120.60
173_V196_A0.5120.60
123_P372_L0.5120.60
134_G141_G0.5110.60
361_L366_L0.5110.60
20_Y48_D0.5110.60
121_F187_R0.5100.60
359_K367_K0.5100.60
98_G231_A0.5100.60
99_S107_I0.5090.60
121_F126_T0.5090.60
160_G383_I0.5090.60
259_G264_R0.5090.60
76_S254_V0.5080.59
280_I283_L0.5080.59
61_L319_C0.5070.59
221_I424_R0.5060.59
255_F266_L0.5050.59
342_L345_A0.5050.59
74_L254_V0.5050.59
7_K376_A0.5050.59
364_A372_L0.5040.59
32_R194_G0.5030.59
143_K196_A0.5020.59
69_G85_I0.5020.59
88_G328_P0.5020.59
18_A299_N0.5020.59
256_Y307_K0.5010.58
52_C243_V0.5000.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3qe7A 2 0.8731 100 0.421 Contact Map
3wdoA 1 0.882 95.3 0.946 Contact Map
4m64A 1 0.8552 94.6 0.948 Contact Map
4pypA 1 0.8864 94 0.95 Contact Map
1pw4A 1 0.8731 92.2 0.953 Contact Map
2cfqA 1 0.8619 91.7 0.953 Contact Map
4j05A 3 0.8508 90.9 0.954 Contact Map
4tphA 2 0.8463 83.9 0.959 Contact Map
4ikvA 1 0.882 82.8 0.96 Contact Map
4ldsA 2 0.8508 80.8 0.961 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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