GREMLIN Database
PNCB - Nicotinate phosphoribosyltransferase
UniProt: O32090 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 490 (446)
Sequences: 1948 (1166)
Seq/√Len: 55.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
193_G200_V4.9311.00
214_D217_T4.8501.00
262_I276_A4.5391.00
78_Q85_E4.2991.00
220_K252_E3.6731.00
65_E426_L3.5771.00
364_K415_D3.5731.00
251_K285_F3.5451.00
64_L100_L3.3291.00
71_D92_R3.2641.00
40_L132_L3.2411.00
60_A132_L3.1881.00
432_E435_D3.1591.00
466_E470_S3.1471.00
419_L422_E3.0821.00
61_I430_N3.0661.00
28_R84_H2.9571.00
163_T172_A2.9561.00
98_G120_A2.8831.00
216_Y220_K2.7731.00
233_V276_A2.7591.00
120_A125_A2.7251.00
233_V243_M2.6361.00
71_D75_S2.6251.00
233_V262_I2.5611.00
186_A201_S2.4141.00
103_M109_V2.3531.00
275_K278_K2.3471.00
233_V279_M2.3071.00
73_D194_K2.2321.00
89_E92_R2.2321.00
291_I313_V2.2101.00
70_T73_D2.2061.00
305_K314_W2.1981.00
51_Y147_A2.1891.00
117_R417_H2.1061.00
40_L60_A2.1031.00
25_T208_L2.1001.00
150_K185_S2.0671.00
99_S423_K2.0361.00
180_L437_Q2.0151.00
230_V261_G2.0001.00
60_A116_M1.9841.00
175_G321_I1.9821.00
301_I316_V1.9761.00
180_L198_I1.9581.00
158_A184_F1.9581.00
121_P124_E1.9391.00
51_Y151_G1.9161.00
289_K312_D1.9091.00
53_V440_V1.9051.00
438_Q442_D1.9011.00
431_P439_Y1.8951.00
254_G258_N1.8871.00
258_N287_D1.8670.99
104_K418_E1.8630.99
101_Y419_L1.8620.99
383_L471_N1.8350.99
156_E314_W1.8090.99
97_T124_E1.7980.99
61_I420_I1.7970.99
54_F57_L1.7960.99
220_K224_E1.7940.99
159_L201_S1.7920.99
216_Y248_R1.7810.99
180_L433_I1.7740.99
156_E305_K1.7670.99
107_E443_N1.7560.99
99_S119_E1.7470.99
250_A259_F1.7460.99
385_D467_D1.7420.99
180_L199_P1.7390.99
42_F45_L1.7360.99
64_L128_I1.7090.99
247_I279_M1.7080.99
65_E425_I1.7070.99
74_L88_I1.7000.99
40_L136_V1.6850.99
359_T472_K1.6820.99
278_K282_E1.6670.99
85_E89_E1.6650.99
277_R281_D1.6640.99
150_K158_A1.6500.99
63_Y131_A1.6500.99
276_A279_M1.6460.99
24_E84_H1.6460.99
89_E93_G1.6370.99
167_H171_A1.6220.98
273_S292_A1.6180.98
119_E362_R1.5850.98
157_V185_S1.5780.98
109_V115_I1.5410.98
304_L309_A1.5350.98
98_G124_E1.5330.98
23_A67_F1.5230.98
90_Y123_V1.5110.98
269_L311_I1.5100.98
434_S437_Q1.4780.97
470_S474_Q1.4780.97
237_D240_R1.4470.97
57_L420_I1.4430.97
150_K155_D1.4430.97
241_S244_P1.4420.97
420_I426_L1.4390.97
244_P279_M1.4260.97
35_K339_E1.4220.97
52_A140_T1.4040.96
75_S79_D1.4010.96
31_I34_K1.3900.96
369_I414_K1.3880.96
101_Y119_E1.3870.96
421_F426_L1.3830.96
103_M365_V1.3790.96
238_T264_L1.3780.96
285_F288_A1.3710.96
80_E200_V1.3710.96
35_K119_E1.3690.96
101_Y422_E1.3670.96
118_V132_L1.3400.95
146_A184_F1.3380.95
191_R194_K1.3370.95
420_I428_Y1.3370.95
61_I426_L1.3150.95
180_L434_S1.3130.95
371_Q412_Y1.3120.95
254_G287_D1.3110.94
386_E465_S1.3060.94
253_F256_R1.3020.94
149_I158_A1.3010.94
67_F94_L1.2980.94
418_E428_Y1.2960.94
72_S194_K1.2930.94
221_K224_E1.2890.94
366_Y382_A1.2860.94
159_L186_A1.2830.94
414_K443_N1.2680.93
159_L313_V1.2640.93
295_D330_G1.2640.93
24_E28_R1.2590.93
128_I132_L1.2580.93
216_Y245_N1.2560.93
53_V108_L1.2550.93
279_M283_A1.2490.93
243_M279_M1.2450.93
29_D221_K1.2440.93
429_Q435_D1.2440.93
453_R457_P1.2430.93
223_A249_V1.2420.93
438_Q441_Q1.2420.93
261_G288_A1.2410.93
61_I421_F1.2390.93
107_E416_L1.2290.92
17_L200_V1.2210.92
397_F469_W1.2190.92
23_A123_V1.2160.92
300_T303_N1.2070.92
230_V291_I1.2050.92
31_I211_A1.2010.91
78_Q92_R1.2000.91
319_K324_Y1.1990.91
297_D319_K1.1920.91
414_K446_L1.1800.91
220_K253_F1.1750.91
393_R409_T1.1580.90
76_Y200_V1.1570.90
426_L430_N1.1510.90
270_A307_Q1.1510.90
47_F323_A1.1500.90
431_P436_I1.1500.90
243_M276_A1.1480.89
254_G285_F1.1410.89
364_K417_H1.1390.89
228_D257_I1.1390.89
217_T221_K1.1320.89
138_Y142_I1.1190.88
28_R86_D1.1190.88
63_Y67_F1.1170.88
174_W196_F1.1160.88
94_L124_E1.1150.88
216_Y252_E1.1110.88
192_A198_I1.1040.88
439_Y443_N1.1020.87
277_R312_D1.0960.87
295_D318_T1.0930.87
17_L190_V1.0890.87
264_L272_L1.0880.87
431_P435_D1.0860.87
382_A465_S1.0800.86
76_Y193_G1.0800.86
39_E363_K1.0770.86
292_A311_I1.0740.86
181_I436_I1.0740.86
99_S422_E1.0720.86
17_L80_E1.0700.86
61_I427_C1.0650.86
50_G457_P1.0600.85
468_C471_N1.0570.85
24_E225_T1.0560.85
148_R298_E1.0510.85
243_M262_I1.0480.85
58_E430_N1.0480.85
45_L52_A1.0460.85
241_S245_N1.0450.85
323_A327_P1.0450.85
220_K249_V1.0430.84
421_F428_Y1.0410.84
131_A135_I1.0400.84
54_F103_M1.0390.84
242_G246_A1.0360.84
36_A122_L1.0350.84
212_Y221_K1.0330.84
318_T328_A1.0330.84
264_L292_A1.0280.84
97_T120_A1.0270.83
242_G249_V1.0260.83
185_S437_Q1.0170.83
193_G199_P1.0170.83
264_L311_I1.0150.83
37_I119_E1.0140.83
100_L128_I1.0080.82
271_Y458_E1.0050.82
104_K439_Y1.0050.82
251_K254_G1.0020.82
58_E62_E0.9970.82
274_K450_E0.9950.81
99_S419_L0.9880.81
419_L427_C0.9870.81
117_R384_Y0.9840.81
414_K418_E0.9800.80
302_M306_A0.9790.80
163_T189_N0.9770.80
443_N446_L0.9720.80
57_L431_P0.9690.80
64_L98_G0.9680.80
440_V443_N0.9680.80
100_L118_V0.9670.80
337_A340_E0.9640.79
137_N331_A0.9640.79
247_I283_A0.9640.79
34_K339_E0.9590.79
369_I446_L0.9550.79
24_E226_H0.9510.78
249_V252_E0.9480.78
101_Y117_R0.9470.78
100_L421_F0.9460.78
251_K255_D0.9440.78
449_E452_K0.9420.78
442_D445_S0.9420.78
450_E458_E0.9410.78
28_R225_T0.9400.78
248_R252_E0.9370.77
215_E269_L0.9350.77
139_Q178_A0.9310.77
437_Q441_Q0.9300.77
361_G471_N0.9290.77
76_Y80_E0.9280.77
138_Y175_G0.9270.77
60_A136_V0.9270.77
60_A131_A0.9220.76
434_S438_Q0.9210.76
215_E245_N0.9190.76
262_I279_M0.9190.76
113_E365_V0.9190.76
432_E438_Q0.9180.76
26_Y126_Q0.9170.76
122_L211_A0.9160.76
240_R244_P0.9120.75
260_I288_A0.9120.75
116_M136_V0.9120.75
250_A254_G0.9060.75
230_V288_A0.9050.75
364_K385_D0.9030.75
197_N434_S0.9020.75
223_A229_C0.9010.75
295_D328_A0.8910.74
191_R195_R0.8880.73
150_K154_G0.8880.73
292_A304_L0.8860.73
162_G166_A0.8840.73
245_N249_V0.8830.73
418_E434_S0.8820.73
150_K184_F0.8810.73
104_K107_E0.8800.73
281_D310_R0.8790.73
63_Y128_I0.8760.72
179_A320_L0.8760.72
117_R362_R0.8760.72
28_R224_E0.8750.72
346_D471_N0.8730.72
208_L212_Y0.8700.72
41_F112_N0.8680.72
21_N83_Y0.8680.72
259_F288_A0.8680.72
95_S98_G0.8650.72
40_L116_M0.8630.71
190_V200_V0.8610.71
29_D224_E0.8600.71
428_Y431_P0.8570.71
451_Y458_E0.8570.71
42_F331_A0.8570.71
80_E193_G0.8560.71
105_E177_R0.8530.70
447_L452_K0.8530.70
103_M416_L0.8510.70
213_R340_E0.8510.70
149_I316_V0.8480.70
61_I428_Y0.8480.70
26_Y36_A0.8460.70
158_A185_S0.8460.70
27_W87_F0.8430.70
366_Y415_D0.8320.69
469_W473_M0.8320.69
34_K340_E0.8310.68
233_V264_L0.8280.68
130_T163_T0.8240.68
259_F285_F0.8240.68
382_A386_E0.8200.67
300_T456_K0.8180.67
233_V244_P0.8150.67
423_K438_Q0.8130.67
445_S452_K0.8120.67
245_N248_R0.8090.66
443_N452_K0.8090.66
15_T20_I0.8070.66
213_R347_T0.8030.66
220_K248_R0.8020.66
376_S460_Y0.8020.66
448_W463_D0.8000.65
42_F52_A0.7970.65
440_V447_L0.7960.65
458_E463_D0.7950.65
279_M282_E0.7930.65
204_H209_V0.7910.65
55_A181_I0.7890.64
365_V417_H0.7890.64
253_F257_I0.7870.64
224_E256_R0.7840.64
182_G440_V0.7830.64
50_G458_E0.7790.63
201_S313_V0.7760.63
435_D438_Q0.7750.63
375_H449_E0.7740.63
109_V140_T0.7740.63
430_N436_I0.7720.63
92_R186_A0.7710.63
59_K139_Q0.7710.63
246_A249_V0.7680.62
458_E461_P0.7620.62
135_I169_M0.7620.62
135_I174_W0.7620.62
78_Q88_I0.7600.61
427_C431_P0.7600.61
74_L92_R0.7590.61
242_G245_N0.7590.61
20_I77_L0.7580.61
156_E312_D0.7540.61
94_L121_P0.7530.61
420_I427_C0.7500.60
65_E430_N0.7490.60
76_Y79_D0.7480.60
77_L193_G0.7430.60
62_E66_N0.7430.60
261_G289_K0.7410.60
370_N377_E0.7410.60
19_Q163_T0.7410.60
201_S291_I0.7400.59
432_E436_I0.7380.59
23_A87_F0.7370.59
304_L311_I0.7360.59
13_L115_I0.7330.59
154_G305_K0.7320.59
52_A329_L0.7270.58
368_I413_A0.7240.58
138_Y166_A0.7230.58
62_E430_N0.7230.58
142_I179_A0.7220.58
39_E117_R0.7210.57
211_A338_I0.7180.57
435_D439_Y0.7150.57
275_K282_E0.7150.57
387_Q390_D0.7140.57
19_Q190_V0.7120.56
75_S78_Q0.7110.56
451_Y460_Y0.7110.56
414_K439_Y0.7090.56
421_F427_C0.7070.56
244_P248_R0.7050.56
32_H123_V0.7040.56
25_T225_T0.7030.56
451_Y461_P0.7020.55
166_A321_I0.7020.55
216_Y249_V0.7010.55
170_D174_W0.6990.55
426_L467_D0.6990.55
55_A143_A0.6970.55
38_F335_L0.6950.55
108_L443_N0.6940.55
83_Y88_I0.6910.54
444_L452_K0.6900.54
60_A128_I0.6900.54
24_E447_L0.6900.54
25_T226_H0.6890.54
65_E421_F0.6850.54
60_A100_L0.6850.54
441_Q445_S0.6810.53
35_K121_P0.6800.53
133_L137_N0.6790.53
389_N393_R0.6730.52
159_L291_I0.6730.52
17_L82_G0.6690.52
20_I83_Y0.6670.52
102_S116_M0.6670.52
40_L331_A0.6660.52
262_I290_V0.6640.51
421_F448_W0.6640.51
15_T19_Q0.6630.51
144_T182_G0.6620.51
418_E467_D0.6610.51
95_S425_I0.6590.51
210_Q235_T0.6590.51
17_L76_Y0.6580.51
12_S63_Y0.6570.51
409_T474_Q0.6560.50
27_W326_Q0.6540.50
71_D89_E0.6520.50
271_Y453_R0.6510.50
247_I262_I0.6510.50
249_V253_F0.6490.50
454_I471_N0.6490.50
210_Q295_D0.6470.50
160_E330_G0.6470.50
152_V302_M0.6460.49
270_A304_L0.6460.49
348_I351_S0.6450.49
296_L301_I0.6450.49
435_D442_D0.6420.49
361_G365_V0.6420.49
391_Q395_R0.6400.49
57_L127_L0.6390.49
162_G321_I0.6380.49
72_S75_S0.6370.48
44_R292_A0.6360.48
142_I320_L0.6360.48
24_E294_S0.6330.48
467_D474_Q0.6330.48
257_I260_I0.6320.48
216_Y253_F0.6310.48
98_G423_K0.6300.48
453_R458_E0.6300.48
415_D419_L0.6290.48
171_A175_G0.6290.48
197_N442_D0.6290.48
393_R410_N0.6280.47
387_Q391_Q0.6270.47
53_V71_D0.6270.47
439_Y442_D0.6260.47
224_E253_F0.6260.47
146_A150_K0.6240.47
318_T325_D0.6240.47
47_F318_T0.6220.47
368_I380_Y0.6220.47
419_L423_K0.6210.47
13_L194_K0.6180.46
173_M191_R0.6180.46
153_I158_A0.6180.46
33_E124_E0.6170.46
41_F449_E0.6170.46
450_E463_D0.6150.46
14_H21_N0.6150.46
130_T330_G0.6140.46
453_R456_K0.6110.46
369_I376_S0.6110.46
368_I389_N0.6110.46
138_Y174_W0.6080.45
64_L222_Y0.6070.45
243_M282_E0.6060.45
442_D446_L0.6050.45
445_S467_D0.6050.45
160_E222_Y0.6040.45
303_N307_Q0.6030.45
371_Q390_D0.6020.45
57_L61_I0.6020.45
152_V184_F0.6000.44
149_I296_L0.6000.44
273_S310_R0.5980.44
452_K460_Y0.5980.44
96_F120_A0.5970.44
248_R256_R0.5950.44
376_S446_L0.5950.44
289_K313_V0.5950.44
14_H163_T0.5930.44
140_T329_L0.5930.44
217_T220_K0.5920.44
54_F115_I0.5920.44
29_D212_Y0.5900.43
442_D470_S0.5900.43
73_D253_F0.5880.43
300_T331_A0.5880.43
60_A135_I0.5880.43
140_T331_A0.5870.43
19_Q130_T0.5870.43
95_S124_E0.5870.43
467_D470_S0.5860.43
14_H107_E0.5860.43
383_L386_E0.5850.43
397_F463_D0.5850.43
388_V413_A0.5840.43
167_H327_P0.5810.42
171_A176_A0.5810.42
376_S447_L0.5780.42
151_G452_K0.5770.42
230_V260_I0.5760.42
59_K168_E0.5740.42
410_N413_A0.5730.42
89_E434_S0.5730.42
75_S85_E0.5720.42
194_K225_T0.5720.42
154_G243_M0.5710.41
233_V283_A0.5700.41
209_V230_V0.5700.41
210_Q460_Y0.5690.41
188_S318_T0.5680.41
414_K435_D0.5680.41
426_L431_P0.5680.41
363_K465_S0.5680.41
117_R295_D0.5680.41
57_L102_S0.5670.41
103_M113_E0.5660.41
223_A253_F0.5650.41
124_E353_N0.5650.41
291_I294_S0.5650.41
418_E442_D0.5640.41
357_V464_L0.5630.41
427_C442_D0.5630.41
302_M455_S0.5630.41
397_F465_S0.5620.40
147_A444_L0.5610.40
175_G179_A0.5610.40
14_H169_M0.5600.40
204_H295_D0.5600.40
57_L104_K0.5590.40
296_L316_V0.5590.40
254_G288_A0.5590.40
19_Q27_W0.5580.40
126_Q227_K0.5580.40
304_L307_Q0.5570.40
238_T300_T0.5560.40
430_N439_Y0.5550.40
39_E321_I0.5550.40
430_N442_D0.5550.40
294_S300_T0.5550.40
249_V254_G0.5550.40
77_L80_E0.5540.40
150_K182_G0.5540.40
116_M132_L0.5540.40
152_V155_D0.5540.40
92_R256_R0.5530.40
37_I314_W0.5530.40
59_K174_W0.5530.40
277_R290_V0.5530.40
397_F464_L0.5510.39
434_S445_S0.5510.39
73_D76_Y0.5510.39
97_T121_P0.5500.39
255_D285_F0.5490.39
420_I431_P0.5490.39
243_M283_A0.5490.39
86_D438_Q0.5490.39
436_I440_V0.5490.39
138_Y167_H0.5490.39
238_T313_V0.5480.39
166_A322_T0.5480.39
292_A357_V0.5480.39
27_W32_H0.5470.39
48_E323_A0.5470.39
41_F474_Q0.5460.39
280_L323_A0.5460.39
314_W325_D0.5450.39
97_T426_L0.5440.39
258_N288_A0.5440.39
271_Y307_Q0.5420.38
130_T172_A0.5420.38
425_I432_E0.5420.38
187_T198_I0.5400.38
295_D319_K0.5400.38
25_T212_Y0.5390.38
47_F325_D0.5390.38
182_G434_S0.5380.38
464_L472_K0.5380.38
97_T410_N0.5380.38
270_A274_K0.5370.38
448_W458_E0.5360.38
29_D225_T0.5360.38
295_D325_D0.5350.38
441_Q444_L0.5340.38
372_S410_N0.5340.38
77_L88_I0.5330.38
303_N306_A0.5330.38
423_K441_Q0.5320.37
99_S429_Q0.5320.37
409_T423_K0.5310.37
232_L235_T0.5310.37
258_N285_F0.5310.37
423_K426_L0.5300.37
152_V310_R0.5300.37
231_F259_F0.5280.37
362_R384_Y0.5270.37
471_N474_Q0.5260.37
464_L468_C0.5250.37
193_G198_I0.5230.37
89_E425_I0.5230.37
463_D469_W0.5230.37
42_F109_V0.5220.36
236_Y416_L0.5220.36
238_T272_L0.5220.36
205_A447_L0.5210.36
24_E81_L0.5210.36
74_L138_Y0.5210.36
27_W47_F0.5210.36
465_S469_W0.5190.36
47_F144_T0.5190.36
39_E336_V0.5190.36
380_Y413_A0.5190.36
19_Q26_Y0.5180.36
163_T188_S0.5180.36
415_D449_E0.5180.36
230_V313_V0.5180.36
139_Q177_R0.5170.36
173_M195_R0.5160.36
158_A320_L0.5160.36
52_A103_M0.5160.36
250_A285_F0.5150.36
418_E445_S0.5150.36
418_E423_K0.5140.36
426_L434_S0.5140.36
147_A151_G0.5130.36
172_A221_K0.5130.36
422_E438_Q0.5120.35
228_D260_I0.5120.35
45_L108_L0.5110.35
149_I184_F0.5110.35
33_E74_L0.5110.35
420_I429_Q0.5100.35
271_Y456_K0.5100.35
397_F466_E0.5100.35
137_N332_V0.5100.35
10_S13_L0.5090.35
74_L211_A0.5080.35
155_D351_S0.5080.35
433_I473_M0.5070.35
262_I273_S0.5060.35
188_S203_T0.5060.35
429_Q442_D0.5050.35
459_E471_N0.5050.35
217_T224_E0.5040.35
453_R463_D0.5040.35
354_P469_W0.5030.35
85_E385_D0.5030.35
364_K384_Y0.5020.34
250_A260_I0.5020.34
69_F74_L0.5020.34
146_A182_G0.5020.34
410_N414_K0.5020.34
425_I438_Q0.5010.34
433_I437_Q0.5010.34
31_I122_L0.5010.34
52_A109_V0.5000.34
161_F186_A0.5000.34
81_L84_H0.5000.34
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4yubA 2 0.9041 100 0.194 Contact Map
4mzyA 2 0.9878 100 0.258 Contact Map
4lvfA 2 0.8245 100 0.349 Contact Map
1ybeA 2 0.7143 100 0.415 Contact Map
3os4A 1 0.7224 100 0.419 Contact Map
1vlpA 1 0.7429 100 0.42 Contact Map
2im5A 1 0.7163 100 0.422 Contact Map
1yirA 1 0.7102 100 0.43 Contact Map
4hl7A 4 0.7408 100 0.444 Contact Map
2i14A 5 0.7714 100 0.477 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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