GREMLIN Database
SAFA - SpoIVD-associated factor A
UniProt: O32062 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 387 (313)
Sequences: 113 (109)
Seq/√Len: 6.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
272_G290_H3.5840.90
186_E189_V3.1090.82
248_G275_G2.7290.72
280_A296_Y2.7180.72
259_E268_H2.7160.71
285_G303_M2.6250.69
251_S285_G2.6020.68
231_Y250_V2.5370.66
231_Y295_P2.5350.66
216_V306_Y2.5020.65
4_H10_D2.4860.64
237_Y302_V2.4480.63
246_Q261_Q2.3530.60
265_N296_Y2.2640.57
309_A314_Y2.2290.55
13_W261_Q2.2160.55
285_G298_G2.1720.53
48_P302_V2.1330.52
237_Y265_N2.0750.50
281_S314_Y2.0650.50
15_I43_M2.0080.47
293_Y305_A1.9960.47
298_G303_M1.9750.46
273_S291_M1.9670.46
251_S298_G1.9650.46
233_P250_V1.9370.45
244_G259_E1.9280.45
21_V246_Q1.8800.43
255_D272_G1.8700.43
265_N302_V1.8680.42
235_Q267_H1.8470.42
4_H19_Y1.8350.41
214_C302_V1.8290.41
214_C285_G1.8110.40
6_V36_P1.8100.40
265_N280_A1.8000.40
48_P265_N1.7730.39
225_S235_Q1.7690.39
237_Y285_G1.7580.39
237_Y280_A1.7560.39
285_G302_V1.7300.38
272_G321_D1.7290.38
267_H281_S1.6920.36
272_G292_P1.6360.35
298_G301_Q1.6280.34
6_V15_I1.6110.34
243_H305_A1.5770.33
222_L309_A1.5720.32
216_V272_G1.5700.32
208_P275_G1.5670.32
171_N177_K1.5410.32
48_P296_Y1.5360.31
255_D292_P1.5270.31
243_H307_Q1.5220.31
48_P280_A1.5070.30
186_E309_A1.5030.30
272_G306_Y1.4980.30
151_M160_A1.4910.30
230_P233_P1.4840.30
7_Q21_V1.4650.29
204_Y234_A1.4570.29
236_A305_A1.4560.29
268_H281_S1.4480.29
189_V222_L1.4460.29
259_E270_H1.4410.28
215_P253_Q1.4390.28
230_P267_H1.4300.28
78_E175_M1.4230.28
214_C266_S1.4160.28
286_S316_P1.4140.28
245_Y293_Y1.3880.27
102_P105_N1.3880.27
301_Q318_K1.3840.27
230_P285_G1.3820.27
247_P268_H1.3600.26
7_Q10_D1.3590.26
216_V220_P1.3550.26
245_Y305_A1.3420.26
6_V9_G1.3300.25
236_A243_H1.3270.25
242_M306_Y1.3250.25
236_A293_Y1.3240.25
272_G320_H1.3190.25
217_P262_H1.3180.25
280_A302_V1.3170.25
271_Y293_Y1.3100.25
296_Y302_V1.3090.25
5_I132_P1.3060.25
267_H285_G1.3000.25
39_I252_P1.2960.24
269_G293_Y1.2960.24
250_V295_P1.2940.24
234_A250_V1.2850.24
269_G286_S1.2850.24
283_A289_G1.2840.24
308_P312_H1.2790.24
22_D237_Y1.2790.24
243_H293_Y1.2660.24
9_G36_P1.2580.23
216_V254_Y1.2570.23
255_D320_H1.2510.23
218_V297_Y1.2510.23
271_Y305_A1.2400.23
29_L47_V1.2350.23
45_I226_G1.2260.23
29_L263_H1.2240.23
292_P321_D1.2100.22
296_Y315_M1.2080.22
26_V163_N1.2050.22
216_V236_A1.2030.22
220_P307_Q1.1960.22
250_V298_G1.1940.22
17_E279_Y1.1920.22
233_P251_S1.1870.22
20_G66_A1.1870.22
251_S303_M1.1830.22
225_S228_C1.1780.21
15_I36_P1.1770.21
224_G315_M1.1720.21
13_W22_D1.1660.21
221_I315_M1.1650.21
256_P293_Y1.1650.21
39_I208_P1.1640.21
236_A306_Y1.1570.21
265_N303_M1.1530.21
241_P293_Y1.1500.21
242_M270_H1.1490.21
7_Q39_I1.1480.21
272_G314_Y1.1470.21
250_V301_Q1.1460.21
236_A289_G1.1410.21
256_P307_Q1.1410.21
19_Y31_T1.1350.20
9_G16_A1.1340.20
231_Y285_G1.1320.20
237_Y303_M1.1270.20
237_Y296_Y1.1250.20
89_T152_Q1.1230.20
193_V318_K1.1210.20
231_Y303_M1.1200.20
13_W246_Q1.1170.20
206_H287_P1.1160.20
244_G296_Y1.1110.20
35_N47_V1.1100.20
37_D253_Q1.1070.20
293_Y307_Q1.1070.20
22_D47_V1.1070.20
239_M258_Y1.1030.20
242_M304_G1.1010.20
47_V234_A1.0990.20
233_P318_K1.0980.20
214_C251_S1.0970.20
43_M264_E1.0880.19
236_A256_P1.0850.19
254_Y293_Y1.0810.19
254_Y306_Y1.0740.19
213_P230_P1.0710.19
214_C282_P1.0700.19
241_P283_A1.0670.19
241_P305_A1.0660.19
116_V122_M1.0660.19
236_A241_P1.0640.19
220_P256_P1.0550.19
224_G301_Q1.0490.18
4_H11_S1.0460.18
286_S289_G1.0460.18
289_G293_Y1.0440.18
235_Q250_V1.0440.18
228_C271_Y1.0410.18
10_D39_I1.0330.18
253_Q285_G1.0310.18
230_P303_M1.0290.18
289_G305_A1.0290.18
186_E202_Q1.0260.18
255_D258_Y1.0220.18
280_A315_M1.0210.18
241_P306_Y1.0210.18
277_P286_S1.0190.18
268_H314_Y1.0150.18
60_A150_H1.0140.18
281_S309_A1.0130.18
235_Q303_M1.0060.18
280_A298_G1.0020.17
233_P285_G1.0020.17
253_Q273_S0.9970.17
202_Q205_Y0.9940.17
191_H267_H0.9910.17
254_Y271_Y0.9910.17
227_L309_A0.9860.17
51_G153_D0.9790.17
13_W260_N0.9630.17
22_D253_Q0.9610.17
285_G301_Q0.9590.17
12_L312_H0.9590.17
236_A271_Y0.9570.17
256_P289_G0.9550.17
243_H256_P0.9380.16
253_Q315_M0.9310.16
83_V176_P0.9280.16
234_A295_P0.9270.16
245_Y256_P0.9270.16
48_P226_G0.9250.16
256_P305_A0.9240.16
216_V255_D0.9240.16
20_G203_P0.9200.16
251_S308_P0.9100.16
269_G316_P0.9060.16
221_I253_Q0.9020.15
236_A307_Q0.9000.15
110_V113_E0.9000.15
230_P298_G0.9000.15
268_H309_A0.8960.15
262_H269_G0.8930.15
168_N171_N0.8920.15
254_Y320_H0.8900.15
267_H303_M0.8840.15
42_G317_Y0.8840.15
41_P268_H0.8830.15
21_V308_P0.8820.15
215_P221_I0.8810.15
206_H274_Y0.8810.15
39_I308_P0.8810.15
252_P315_M0.8770.15
76_A128_P0.8770.15
215_P267_H0.8750.15
233_P266_S0.8720.15
298_G315_M0.8690.15
217_P270_H0.8650.15
181_V184_I0.8630.15
22_D303_M0.8630.15
78_E96_S0.8620.15
217_P269_G0.8620.15
280_A293_Y0.8620.15
170_P177_K0.8620.15
235_Q281_S0.8610.15
232_Y238_P0.8570.15
303_M315_M0.8560.15
281_S315_M0.8550.15
231_Y234_A0.8550.15
34_S64_P0.8510.15
189_V192_T0.8490.15
15_I26_V0.8490.15
216_V320_H0.8460.14
42_G213_P0.8450.14
215_P285_G0.8440.14
250_V267_H0.8380.14
9_G15_I0.8330.14
245_Y307_Q0.8300.14
260_N282_P0.8290.14
231_Y235_Q0.8280.14
211_F266_S0.8270.14
251_S315_M0.8270.14
14_K291_M0.8260.14
193_V297_Y0.8210.14
306_Y320_H0.8200.14
236_A320_H0.8190.14
250_V303_M0.8100.14
224_G318_K0.8090.14
92_K152_Q0.8080.14
230_P318_K0.8070.14
10_D30_N0.8020.14
279_Y313_G0.8010.14
285_G312_H0.8010.14
216_V290_H0.8000.14
249_F293_Y0.7990.14
39_I279_Y0.7990.14
15_I253_Q0.7990.14
21_V48_P0.7980.14
231_Y266_S0.7980.14
250_V285_G0.7980.14
37_D47_V0.7970.14
247_P309_A0.7960.14
44_K219_S0.7960.14
221_I224_G0.7940.14
299_T318_K0.7910.13
16_A272_G0.7900.13
254_Y272_G0.7880.13
272_G281_S0.7840.13
236_A249_F0.7830.13
256_P271_Y0.7830.13
256_P295_P0.7820.13
250_V253_Q0.7820.13
5_I33_L0.7770.13
216_V242_M0.7730.13
214_C234_A0.7700.13
215_P251_S0.7690.13
95_K98_P0.7690.13
40_M46_K0.7680.13
10_D15_I0.7670.13
313_G317_Y0.7670.13
13_W23_V0.7670.13
249_F286_S0.7660.13
258_Y314_Y0.7650.13
224_G303_M0.7650.13
294_G303_M0.7640.13
224_G230_P0.7630.13
251_S258_Y0.7630.13
256_P280_A0.7570.13
290_H321_D0.7560.13
253_Q291_M0.7540.13
265_N285_G0.7500.13
243_H271_Y0.7470.13
5_I10_D0.7470.13
249_F305_A0.7460.13
237_Y281_S0.7450.13
47_V215_P0.7440.13
285_G297_Y0.7420.13
303_M310_A0.7420.13
230_P250_V0.7410.13
230_P266_S0.7390.13
233_P252_P0.7380.13
259_E298_G0.7380.13
48_P237_Y0.7320.13
283_A293_Y0.7310.13
259_E285_G0.7260.12
225_S285_G0.7230.12
258_Y280_A0.7220.12
22_D45_I0.7210.12
269_G277_P0.7210.12
159_E172_M0.7180.12
13_W24_E0.7140.12
233_P303_M0.7140.12
22_D214_C0.7120.12
214_C303_M0.7110.12
237_Y258_Y0.7090.12
285_G318_K0.7070.12
227_L257_G0.7070.12
111_Y135_E0.7060.12
194_P235_Q0.7060.12
239_M309_A0.7040.12
236_A283_A0.7030.12
315_M318_K0.7020.12
269_G305_A0.7010.12
14_K47_V0.6990.12
251_S302_V0.6970.12
280_A285_G0.6940.12
230_P299_T0.6930.12
4_H15_I0.6900.12
262_H305_A0.6900.12
237_Y251_S0.6890.12
250_V315_M0.6890.12
219_S319_D0.6870.12
273_S301_Q0.6860.12
253_Q298_G0.6820.12
231_Y301_Q0.6820.12
270_H283_A0.6790.12
220_P306_Y0.6780.12
194_P226_G0.6690.12
241_P256_P0.6670.12
252_P274_Y0.6620.11
193_V315_M0.6600.11
14_K280_A0.6590.11
66_A163_N0.6570.11
9_G30_N0.6540.11
7_Q246_Q0.6520.11
148_M159_E0.6510.11
216_V283_A0.6500.11
226_G233_P0.6500.11
93_E128_P0.6490.11
42_G319_D0.6480.11
227_L307_Q0.6480.11
22_D285_G0.6440.11
175_M182_G0.6440.11
215_P296_Y0.6440.11
267_H302_V0.6430.11
250_V266_S0.6420.11
251_S282_P0.6420.11
191_H318_K0.6400.11
265_N298_G0.6360.11
253_Q303_M0.6360.11
16_A292_P0.6330.11
252_P308_P0.6300.11
30_N217_P0.6290.11
239_M317_Y0.6290.11
48_P214_C0.6270.11
11_S90_K0.6260.11
245_Y254_Y0.6260.11
46_K313_G0.6260.11
262_H293_Y0.6230.11
292_P303_M0.6230.11
256_P286_S0.6210.11
231_Y251_S0.6200.11
184_I313_G0.6180.11
39_I146_D0.6170.11
254_Y305_A0.6150.11
250_V268_H0.6120.11
48_P194_P0.6100.11
234_A318_K0.6100.11
298_G302_V0.6100.11
253_Q261_Q0.6090.11
285_G292_P0.6090.11
288_Y291_M0.6050.11
234_A315_M0.6010.11
250_V299_T0.5990.11
256_P304_G0.5990.11
243_H289_G0.5980.11
253_Q268_H0.5950.11
37_D309_A0.5940.11
292_P298_G0.5940.11
18_K174_N0.5930.10
236_A245_Y0.5920.10
237_Y274_Y0.5920.10
84_V116_V0.5910.10
230_P251_S0.5890.10
274_Y280_A0.5890.10
231_Y314_Y0.5890.10
216_V307_Q0.5880.10
47_V253_Q0.5860.10
68_S128_P0.5850.10
242_M283_A0.5830.10
161_M165_E0.5830.10
251_S274_Y0.5830.10
242_M277_P0.5810.10
94_K98_P0.5790.10
294_G301_Q0.5770.10
16_A255_D0.5740.10
281_S303_M0.5740.10
237_Y267_H0.5710.10
69_V154_Q0.5680.10
30_N220_P0.5670.10
22_D37_D0.5660.10
270_H307_Q0.5640.10
118_D130_S0.5620.10
253_Q267_H0.5600.10
231_Y318_K0.5580.10
264_E292_P0.5580.10
235_Q318_K0.5580.10
217_P277_P0.5560.10
237_Y263_H0.5550.10
22_D273_S0.5550.10
5_I14_K0.5510.10
48_P285_G0.5500.10
30_N262_H0.5480.10
261_Q268_H0.5470.10
241_P307_Q0.5460.10
36_P292_P0.5460.10
271_Y307_Q0.5450.10
283_A307_Q0.5420.10
267_H318_K0.5380.10
298_G318_K0.5380.10
251_S299_T0.5360.10
262_H307_Q0.5360.10
6_V30_N0.5350.10
13_W38_L0.5350.10
7_Q198_M0.5330.10
231_Y315_M0.5310.10
241_P271_Y0.5310.10
13_W37_D0.5270.10
289_G307_Q0.5270.10
235_Q302_V0.5250.10
230_P281_S0.5240.10
17_E302_V0.5240.10
308_P315_M0.5230.10
271_Y289_G0.5220.10
227_L258_Y0.5210.10
196_M315_M0.5210.10
264_E274_Y0.5210.10
270_H316_P0.5200.10
304_G313_G0.5160.09
277_P320_H0.5150.09
267_H307_Q0.5130.09
285_G315_M0.5130.09
289_G292_P0.5090.09
104_P166_N0.5070.09
250_V318_K0.5060.09
242_M246_Q0.5030.09
255_D270_H0.5020.09
234_A251_S0.5020.09
266_S272_G0.5010.09
262_H316_P0.5000.09
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2gu1A 1 0.7494 100 0.72 Contact Map
3sluA 1 0.8269 100 0.747 Contact Map
1qwyA 1 0.3592 100 0.769 Contact Map
4bh5A 4 0.2946 100 0.783 Contact Map
4rnzA 1 0.8424 100 0.791 Contact Map
2hsiA 1 0.4264 99.9 0.798 Contact Map
3tufB 1 0.3101 99.9 0.801 Contact Map
4qp5A 1 0.2817 99.9 0.802 Contact Map
4lxcA 4 0.4522 99.9 0.804 Contact Map
3nyyA 2 0.5116 99.9 0.824 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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