TGT - BACSU - GREMLIN Contacts

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TGT - Queuine tRNA-ribosyltransferase

UniProt:	O32053	Sequences: MAEQPIRYEFIKECKQTGARLGKVHTPHGSFETPVFMPVGTLATVKTMSPEELKAMDAGIILSNTYHLWLRPGQDIVKEAGGLHKFMNWDRAILTDSGGFQVFSLSKFRNIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPAEYDYMKRSVERTSRWAERCLNAHNRQDEQGLFGIVQGGEYEDLRTQSAKDLISLDFPGYAIGGLSVGEPKDVMNRVLEFTTPLLPKDKPRYLMGVGSPDALIDGAIRGVDMFDCVLPTRIARNGTVFTAEGRLNMKNAKFERDFRPIDEECDCYTCKNYTRAYIRHLIRCNETFGLRLTTYHNLHFLLHLMEQVRQAIREDRLGDFREEFFERYGYNKPNAKSF	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	381 (362)
Sequences:	3221 (1652)
Seq/√Len:	86.8

ECOLI - TGT
BACSU - TGT
HALSA - ATGT
SULSO - ATGT

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
21_L	356_R	4.079	1.00
54_K	90_D	3.348	1.00
159_Y	199_D	3.286	1.00
79_E	299_R	3.222	1.00
174_E	178_N	3.037	1.00
74_Q	140_A	3.024	1.00
113_E	171_R	2.936	1.00
283_V	336_T	2.873	1.00
309_D	342_H	2.841	1.00
262_A	351_V	2.632	1.00
163_K	167_E	2.629	1.00
308_C	313_C	2.626	1.00
353_Q	357_E	2.609	1.00
23_K	32_E	2.607	1.00
263_I	364_R	2.598	1.00
52_E	334_R	2.531	1.00
207_D	210_S	2.512	1.00
78_K	140_A	2.481	1.00
80_A	301_F	2.431	1.00
23_K	30_S	2.417	1.00
32_E	59_G	2.365	1.00
14_C	17_T	2.351	1.00
202_T	206_K	2.278	1.00
170_S	204_S	2.260	1.00
170_S	207_D	2.195	1.00
54_K	91_R	2.128	1.00
132_E	175_R	2.125	1.00
309_D	346_H	2.121	1.00
229_V	232_R	2.117	1.00
159_Y	163_K	2.113	1.00
302_R	306_E	2.091	1.00
203_Q	207_D	2.090	1.00
310_C	313_C	2.031	1.00
9_E	23_K	2.025	1.00
173_A	213_F	2.016	1.00
263_I	360_L	2.006	1.00
47_T	330_T	1.996	1.00
287_E	370_R	1.969	1.00
25_H	30_S	1.964	1.00
235_E	264_R	1.954	1.00
302_R	318_R	1.954	1.00
295_A	298_E	1.944	1.00
351_V	360_L	1.935	1.00
133_K	136_E	1.932	1.00
69_W	127_L	1.903	1.00
130_S	133_K	1.900	1.00
262_A	348_M	1.888	1.00
205_A	237_T	1.878	1.00
158_E	161_Y	1.868	1.00
9_E	25_H	1.851	1.00
218_I	266_V	1.842	1.00
202_T	240_L	1.830	1.00
11_I	23_K	1.826	1.00
297_F	300_D	1.816	1.00
198_E	232_R	1.809	1.00
192_V	241_L	1.808	1.00
162_M	195_G	1.800	1.00
77_V	141_L	1.767	1.00
163_K	203_Q	1.764	1.00
151_E	161_Y	1.757	1.00
167_E	203_Q	1.738	1.00
136_E	179_A	1.731	1.00
179_A	182_R	1.723	1.00
29_G	92_A	1.697	1.00
119_R	126_K	1.683	1.00
143_S	146_M	1.661	1.00
43_A	87_M	1.650	1.00
342_H	346_H	1.640	1.00
174_E	211_L	1.640	1.00
350_Q	363_F	1.638	1.00
286_A	350_Q	1.627	1.00
56_M	341_L	1.624	1.00
83_L	93_I	1.596	1.00
346_H	349_E	1.593	1.00
312_T	338_Y	1.591	1.00
51_E	316_Y	1.579	1.00
75_D	140_A	1.575	1.00
173_A	208_L	1.572	1.00
311_Y	315_N	1.551	1.00
259_I	351_V	1.551	1.00
153_P	162_M	1.534	1.00
226_P	229_V	1.534	1.00
283_V	292_M	1.516	1.00
51_E	55_A	1.513	1.00
21_L	32_E	1.494	1.00
366_E	370_R	1.486	0.99
120_N	123_N	1.484	0.99
275_T	337_T	1.482	0.99
190_G	213_F	1.474	0.99
148_A	176_C	1.469	0.99
49_S	52_E	1.456	0.99
259_I	364_R	1.451	0.99
260_D	264_R	1.450	0.99
302_R	317_T	1.417	0.99
347_L	363_F	1.406	0.99
35_V	345_L	1.404	0.99
198_E	236_F	1.399	0.99
16_Q	358_D	1.398	0.99
117_H	126_K	1.381	0.99
24_V	31_F	1.381	0.99
139_N	183_Q	1.376	0.99
7_R	25_H	1.370	0.99
202_T	236_F	1.366	0.99
231_N	260_D	1.350	0.99
50_P	316_Y	1.350	0.99
88_N	316_Y	1.346	0.99
283_V	304_I	1.333	0.99
49_S	334_R	1.325	0.99
84_H	93_I	1.321	0.99
330_T	333_L	1.318	0.99
76_I	299_R	1.310	0.99
307_E	370_R	1.307	0.99
325_I	336_T	1.305	0.99
32_E	356_R	1.305	0.99
60_I	145_I	1.296	0.99
363_F	367_F	1.291	0.99
282_T	289_R	1.279	0.98
134_A	138_Q	1.271	0.98
322_R	326_R	1.263	0.98
346_H	350_Q	1.250	0.98
258_L	348_M	1.232	0.98
205_A	240_L	1.232	0.98
311_Y	338_Y	1.226	0.98
227_K	257_A	1.223	0.98
15_K	243_K	1.219	0.98
249_L	266_V	1.207	0.98
117_H	128_F	1.206	0.98
112_E	128_F	1.198	0.98
148_A	173_A	1.197	0.98
249_L	261_G	1.195	0.98
286_A	307_E	1.194	0.98
189_F	268_M	1.186	0.97
277_I	280_N	1.183	0.97
11_I	21_L	1.176	0.97
139_N	181_N	1.176	0.97
162_M	197_Y	1.175	0.97
353_Q	356_R	1.170	0.97
258_L	351_V	1.170	0.97
349_E	353_Q	1.169	0.97
151_E	165_S	1.165	0.97
177_L	211_L	1.161	0.97
325_I	332_G	1.160	0.97
286_A	346_H	1.153	0.97
88_N	315_N	1.145	0.97
206_K	210_S	1.137	0.97
18_G	239_P	1.133	0.97
354_A	359_R	1.131	0.97
208_L	216_Y	1.128	0.97
15_K	358_D	1.124	0.96
42_L	327_C	1.116	0.96
135_M	146_M	1.108	0.96
249_L	252_V	1.102	0.96
69_W	74_Q	1.099	0.96
308_C	339_H	1.097	0.96
177_L	181_N	1.092	0.96
359_R	362_D	1.089	0.96
263_I	361_G	1.088	0.96
233_V	236_F	1.086	0.96
148_A	169_T	1.081	0.96
203_Q	206_K	1.080	0.96
26_T	29_G	1.080	0.96
300_D	318_R	1.079	0.96
342_H	349_E	1.077	0.95
142_G	186_Q	1.073	0.95
286_A	370_R	1.067	0.95
160_D	164_R	1.062	0.95
160_D	163_K	1.060	0.95
174_E	210_S	1.060	0.95
83_L	141_L	1.058	0.95
212_D	245_K	1.058	0.95
16_Q	355_I	1.057	0.95
134_A	149_F	1.054	0.95
313_C	339_H	1.051	0.95
133_K	137_I	1.049	0.95
205_A	241_L	1.046	0.95
161_Y	165_S	1.045	0.95
139_N	180_H	1.044	0.95
312_T	321_I	1.039	0.95
111_I	149_F	1.038	0.94
48_M	341_L	1.037	0.94
273_L	284_F	1.035	0.94
136_E	140_A	1.034	0.94
198_E	202_T	1.031	0.94
155_Y	226_P	1.028	0.94
135_M	176_C	1.024	0.94
43_A	63_S	1.019	0.94
35_V	268_M	1.003	0.93
129_L	134_A	1.001	0.93
69_W	129_L	1.001	0.93
292_M	325_I	0.999	0.93
159_Y	197_Y	0.995	0.93
26_T	31_F	0.995	0.93
285_T	288_G	0.995	0.93
234_L	261_G	0.990	0.93
157_A	195_G	0.978	0.93
93_I	143_S	0.978	0.93
53_L	56_M	0.978	0.93
80_A	319_A	0.977	0.92
260_D	368_F	0.974	0.92
177_L	214_P	0.974	0.92
58_A	345_L	0.973	0.92
159_Y	162_M	0.970	0.92
170_S	208_L	0.968	0.92
153_P	166_V	0.968	0.92
166_V	170_S	0.967	0.92
177_L	213_F	0.966	0.92
298_E	322_R	0.961	0.92
112_E	117_H	0.959	0.92
286_A	305_D	0.957	0.92
76_I	79_E	0.952	0.91
173_A	211_L	0.951	0.91
293_K	325_I	0.937	0.91
273_L	344_L	0.934	0.91
290_L	297_F	0.933	0.91
155_Y	196_E	0.921	0.90
237_T	240_L	0.920	0.90
278_A	338_Y	0.919	0.90
209_I	216_Y	0.915	0.90
138_Q	143_S	0.913	0.90
87_M	93_I	0.912	0.90
156_P	195_G	0.910	0.90
259_I	367_F	0.908	0.89
135_M	149_F	0.907	0.89
66_Y	121_H	0.902	0.89
49_S	331_F	0.900	0.89
48_M	338_Y	0.898	0.89
147_M	191_I	0.894	0.89
30_S	59_G	0.891	0.89
283_V	321_I	0.890	0.88
312_T	315_N	0.890	0.88
109_R	151_E	0.890	0.88
59_G	92_A	0.888	0.88
254_S	273_L	0.884	0.88
204_S	208_L	0.884	0.88
351_V	354_A	0.881	0.88
45_V	53_L	0.880	0.88
365_E	369_E	0.879	0.88
309_D	349_E	0.877	0.88
161_Y	164_R	0.876	0.88
198_E	233_V	0.855	0.86
63_S	83_L	0.853	0.86
114_E	128_F	0.851	0.86
206_K	240_L	0.848	0.86
204_S	207_D	0.841	0.86
290_L	318_R	0.840	0.85
284_F	344_L	0.840	0.85
16_Q	361_G	0.836	0.85
263_I	368_F	0.836	0.85
70_L	122_L	0.836	0.85
256_D	368_F	0.832	0.85
56_M	345_L	0.831	0.85
300_D	319_A	0.830	0.85
174_E	207_D	0.825	0.84
359_R	366_E	0.825	0.84
362_D	366_E	0.823	0.84
85_K	88_N	0.822	0.84
354_A	360_L	0.822	0.84
207_D	211_L	0.821	0.84
260_D	364_R	0.821	0.84
286_A	343_F	0.818	0.84
235_E	238_T	0.817	0.84
102_V	149_F	0.814	0.84
228_D	232_R	0.814	0.84
187_G	214_P	0.809	0.83
360_L	364_R	0.808	0.83
192_V	205_A	0.805	0.83
61_I	87_M	0.804	0.83
182_R	185_E	0.800	0.83
259_I	363_F	0.796	0.82
85_K	90_D	0.796	0.82
59_G	91_R	0.796	0.82
218_I	221_L	0.794	0.82
324_L	331_F	0.792	0.82
227_K	230_M	0.785	0.81
323_H	329_E	0.784	0.81
192_V	208_L	0.783	0.81
14_C	358_D	0.782	0.81
166_V	203_Q	0.782	0.81
301_F	317_T	0.781	0.81
147_M	250_M	0.780	0.81
301_F	319_A	0.779	0.81
10_F	21_L	0.778	0.81
166_V	204_S	0.778	0.81
102_V	116_V	0.776	0.81
343_F	347_L	0.775	0.81
281_G	325_I	0.773	0.80
292_M	336_T	0.772	0.80
170_S	211_L	0.767	0.80
205_A	209_I	0.766	0.80
31_F	35_V	0.764	0.80
258_L	261_G	0.763	0.79
311_Y	316_Y	0.761	0.79
48_M	53_L	0.760	0.79
237_T	241_L	0.760	0.79
361_G	365_E	0.758	0.79
272_V	276_R	0.758	0.79
76_I	80_A	0.756	0.79
117_H	130_S	0.755	0.79
277_I	282_T	0.754	0.79
137_I	141_L	0.751	0.78
320_Y	323_H	0.750	0.78
135_M	148_A	0.749	0.78
35_V	60_I	0.749	0.78
351_V	363_F	0.747	0.78
69_W	133_K	0.744	0.78
81_G	85_K	0.744	0.78
157_A	162_M	0.742	0.78
283_V	325_I	0.740	0.77
221_L	225_E	0.740	0.77
48_M	337_T	0.739	0.77
107_K	119_R	0.735	0.77
135_M	139_N	0.735	0.77
151_E	168_R	0.729	0.76
196_E	222_S	0.727	0.76
164_R	168_R	0.724	0.76
50_P	311_Y	0.723	0.76
277_I	284_F	0.723	0.76
162_M	200_L	0.720	0.75
310_C	335_L	0.720	0.75
47_T	334_R	0.719	0.75
238_T	264_R	0.719	0.75
196_E	229_V	0.718	0.75
170_S	210_S	0.714	0.75
321_I	325_I	0.713	0.75
50_P	87_M	0.712	0.75
312_T	316_Y	0.712	0.75
16_Q	362_D	0.710	0.74
211_L	214_P	0.709	0.74
196_E	233_V	0.709	0.74
321_I	336_T	0.704	0.74
328_N	333_L	0.704	0.74
57_D	349_E	0.703	0.74
313_C	335_L	0.702	0.74
178_N	182_R	0.701	0.73
51_E	88_N	0.699	0.73
308_C	335_L	0.697	0.73
116_V	149_F	0.695	0.73
48_M	334_R	0.695	0.73
74_Q	136_E	0.694	0.73
177_L	212_D	0.694	0.73
304_I	336_T	0.694	0.73
164_R	167_E	0.693	0.73
310_C	339_H	0.692	0.73
21_L	351_V	0.690	0.72
334_R	338_Y	0.690	0.72
202_T	239_P	0.689	0.72
177_L	216_Y	0.687	0.72
11_I	356_R	0.687	0.72
17_T	361_G	0.686	0.72
332_G	336_T	0.686	0.72
281_G	292_M	0.683	0.72
205_A	238_T	0.683	0.72
71_R	122_L	0.682	0.71
7_R	30_S	0.678	0.71
128_F	133_K	0.677	0.71
254_S	344_L	0.672	0.70
308_C	315_N	0.671	0.70
26_T	92_A	0.670	0.70
22_G	33_T	0.669	0.70
57_D	91_R	0.667	0.70
156_P	196_E	0.667	0.70
18_G	235_E	0.664	0.69
37_M	60_I	0.663	0.69
236_F	240_L	0.662	0.69
153_P	196_E	0.660	0.69
234_L	238_T	0.660	0.69
273_L	277_I	0.659	0.69
21_L	360_L	0.658	0.69
135_M	188_L	0.657	0.69
42_L	71_R	0.656	0.68
354_A	363_F	0.654	0.68
23_K	59_G	0.654	0.68
364_R	368_F	0.653	0.68
350_Q	353_Q	0.652	0.68
139_N	146_M	0.652	0.68
259_I	360_L	0.651	0.68
14_C	361_G	0.651	0.68
181_N	184_D	0.650	0.68
277_I	283_V	0.644	0.67
192_V	237_T	0.644	0.67
9_E	32_E	0.643	0.67
254_S	257_A	0.643	0.67
238_T	247_R	0.642	0.67
325_I	333_L	0.640	0.67
216_Y	245_K	0.640	0.67
229_V	233_V	0.640	0.67
153_P	165_S	0.636	0.66
136_E	182_R	0.628	0.65
291_N	294_N	0.627	0.65
147_M	248_Y	0.626	0.65
246_P	268_M	0.624	0.65
26_T	186_Q	0.624	0.65
308_C	342_H	0.623	0.64
222_S	225_E	0.622	0.64
27_P	187_G	0.620	0.64
320_Y	324_L	0.620	0.64
218_I	237_T	0.619	0.64
47_T	333_L	0.618	0.64
277_I	336_T	0.618	0.64
74_Q	77_V	0.618	0.64
134_A	176_C	0.615	0.64
40_G	45_V	0.613	0.63
259_I	347_L	0.613	0.63
177_L	210_S	0.611	0.63
36_F	274_P	0.610	0.63
80_A	317_T	0.609	0.63
9_E	30_S	0.608	0.63
155_Y	195_G	0.607	0.62
261_G	269_F	0.606	0.62
146_M	180_H	0.606	0.62
292_M	321_I	0.604	0.62
129_L	133_K	0.603	0.62
338_Y	342_H	0.602	0.62
113_E	168_R	0.601	0.62
290_L	302_R	0.601	0.62
295_A	322_R	0.601	0.62
231_N	257_A	0.600	0.62
69_W	137_I	0.600	0.62
312_T	342_H	0.597	0.61
202_T	205_A	0.596	0.61
76_I	298_E	0.595	0.61
94_L	147_M	0.594	0.61
281_G	293_K	0.593	0.61
280_N	330_T	0.593	0.61
286_A	304_I	0.592	0.61
143_S	148_A	0.591	0.60
43_A	83_L	0.589	0.60
156_P	226_P	0.589	0.60
53_L	57_D	0.589	0.60
77_V	299_R	0.588	0.60
233_V	237_T	0.587	0.60
165_S	168_R	0.586	0.60
28_H	82_G	0.586	0.60
51_E	315_N	0.584	0.60
35_V	59_G	0.583	0.59
179_A	183_Q	0.580	0.59
74_Q	137_I	0.579	0.59
55_A	314_K	0.579	0.59
283_V	290_L	0.578	0.59
167_E	171_R	0.577	0.59
208_L	213_F	0.574	0.58
191_I	250_M	0.572	0.58
320_Y	329_E	0.572	0.58
308_C	312_T	0.571	0.58
69_W	125_D	0.569	0.58
111_I	161_Y	0.568	0.57
287_E	307_E	0.565	0.57
225_E	248_Y	0.565	0.57
31_F	59_G	0.564	0.57
256_D	367_F	0.564	0.57
287_E	306_E	0.562	0.57
262_A	269_F	0.561	0.57
238_T	241_L	0.561	0.57
18_G	243_K	0.560	0.56
337_T	344_L	0.560	0.56
146_M	186_Q	0.560	0.56
56_M	342_H	0.559	0.56
259_I	262_A	0.559	0.56
259_I	368_F	0.558	0.56
259_I	343_F	0.558	0.56
37_M	94_L	0.558	0.56
276_R	279_R	0.558	0.56
148_A	213_F	0.557	0.56
232_R	236_F	0.555	0.56
162_M	166_V	0.554	0.56
348_M	351_V	0.552	0.55
218_I	238_T	0.551	0.55
227_K	231_N	0.550	0.55
350_Q	354_A	0.549	0.55
37_M	147_M	0.549	0.55
235_E	239_P	0.548	0.55
129_L	137_I	0.545	0.54
345_L	349_E	0.545	0.54
227_K	256_D	0.545	0.54
47_T	278_A	0.545	0.54
159_Y	200_L	0.542	0.54
170_S	209_I	0.541	0.54
61_I	93_I	0.540	0.54
146_M	149_F	0.540	0.54
139_N	179_A	0.539	0.54
82_G	85_K	0.538	0.53
168_R	171_R	0.538	0.53
219_G	225_E	0.538	0.53
50_P	88_N	0.537	0.53
79_E	301_F	0.537	0.53
292_M	332_G	0.536	0.53
31_F	92_A	0.536	0.53
180_H	186_Q	0.535	0.53
180_H	214_P	0.534	0.53
79_E	319_A	0.533	0.53
53_L	58_A	0.533	0.53
282_T	291_N	0.533	0.53
304_I	312_T	0.532	0.53
313_C	342_H	0.531	0.52
258_L	344_L	0.531	0.52
208_L	211_L	0.530	0.52
305_D	346_H	0.530	0.52
354_A	358_D	0.529	0.52
64_N	68_L	0.528	0.52
310_C	342_H	0.528	0.52
256_D	289_R	0.527	0.52
51_E	56_M	0.526	0.52
280_N	325_I	0.526	0.52
277_I	344_L	0.525	0.52
27_P	180_H	0.525	0.52
103_F	222_S	0.524	0.52
198_E	240_L	0.524	0.52
335_L	339_H	0.524	0.52
28_H	186_Q	0.523	0.51
194_G	201_R	0.523	0.51
108_F	121_H	0.522	0.51
44_T	331_F	0.522	0.51
109_R	165_S	0.522	0.51
227_K	278_A	0.521	0.51
177_L	180_H	0.521	0.51
50_P	312_T	0.521	0.51
343_F	368_F	0.520	0.51
293_K	332_G	0.518	0.51
113_E	175_R	0.515	0.50
110_N	117_H	0.515	0.50
94_L	250_M	0.514	0.50
60_I	94_L	0.514	0.50
96_D	150_D	0.513	0.50
113_E	165_S	0.511	0.50
106_S	109_R	0.510	0.50
231_N	264_R	0.510	0.50
32_E	349_E	0.510	0.50
77_V	80_A	0.510	0.50
212_D	216_Y	0.510	0.50
238_T	266_V	0.510	0.50
48_M	330_T	0.509	0.50
354_A	357_E	0.507	0.49
166_V	169_T	0.506	0.49
20_R	247_R	0.506	0.49
209_I	240_L	0.506	0.49
155_Y	162_M	0.506	0.49
213_F	216_Y	0.504	0.49
228_D	237_T	0.502	0.49
203_Q	210_S	0.502	0.49
361_G	364_R	0.501	0.48
14_C	356_R	0.501	0.48
51_E	334_R	0.500	0.48
122_L	327_C	0.500	0.48

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4q8wA	2	0.9606	100	0.069	Contact Map
2ashA	3	0.9475	100	0.089	Contact Map
1iq8A	2	0.895	100	0.203	Contact Map
1ydnA	1	0.6798	89.6	0.956	Contact Map
3oixA	3	0.7087	87.2	0.958	Contact Map
3t7vA	1	0.7139	86.1	0.959	Contact Map
4xp7A	1	0.7979	85	0.959	Contact Map
4oqvA	1	0.7533	83.1	0.96	Contact Map
1ydoA	3	0.685	82.6	0.96	Contact Map
1vcfA	3	0.7218	82.1	0.96	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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