GREMLIN Database
TGT - Queuine tRNA-ribosyltransferase
UniProt: O32053 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 381 (362)
Sequences: 3221 (1652)
Seq/√Len: 86.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
21_L356_R4.0791.00
54_K90_D3.3481.00
159_Y199_D3.2861.00
79_E299_R3.2221.00
174_E178_N3.0371.00
74_Q140_A3.0241.00
113_E171_R2.9361.00
283_V336_T2.8731.00
309_D342_H2.8411.00
262_A351_V2.6321.00
163_K167_E2.6291.00
308_C313_C2.6261.00
353_Q357_E2.6091.00
23_K32_E2.6071.00
263_I364_R2.5981.00
52_E334_R2.5311.00
207_D210_S2.5121.00
78_K140_A2.4811.00
80_A301_F2.4311.00
23_K30_S2.4171.00
32_E59_G2.3651.00
14_C17_T2.3511.00
202_T206_K2.2781.00
170_S204_S2.2601.00
170_S207_D2.1951.00
54_K91_R2.1281.00
132_E175_R2.1251.00
309_D346_H2.1211.00
229_V232_R2.1171.00
159_Y163_K2.1131.00
302_R306_E2.0911.00
203_Q207_D2.0901.00
310_C313_C2.0311.00
9_E23_K2.0251.00
173_A213_F2.0161.00
263_I360_L2.0061.00
47_T330_T1.9961.00
287_E370_R1.9691.00
25_H30_S1.9641.00
235_E264_R1.9541.00
302_R318_R1.9541.00
295_A298_E1.9441.00
351_V360_L1.9351.00
133_K136_E1.9321.00
69_W127_L1.9031.00
130_S133_K1.9001.00
262_A348_M1.8881.00
205_A237_T1.8781.00
158_E161_Y1.8681.00
9_E25_H1.8511.00
218_I266_V1.8421.00
202_T240_L1.8301.00
11_I23_K1.8261.00
297_F300_D1.8161.00
198_E232_R1.8091.00
192_V241_L1.8081.00
162_M195_G1.8001.00
77_V141_L1.7671.00
163_K203_Q1.7641.00
151_E161_Y1.7571.00
167_E203_Q1.7381.00
136_E179_A1.7311.00
179_A182_R1.7231.00
29_G92_A1.6971.00
119_R126_K1.6831.00
143_S146_M1.6611.00
43_A87_M1.6501.00
342_H346_H1.6401.00
174_E211_L1.6401.00
350_Q363_F1.6381.00
286_A350_Q1.6271.00
56_M341_L1.6241.00
83_L93_I1.5961.00
346_H349_E1.5931.00
312_T338_Y1.5911.00
51_E316_Y1.5791.00
75_D140_A1.5751.00
173_A208_L1.5721.00
311_Y315_N1.5511.00
259_I351_V1.5511.00
153_P162_M1.5341.00
226_P229_V1.5341.00
283_V292_M1.5161.00
51_E55_A1.5131.00
21_L32_E1.4941.00
366_E370_R1.4860.99
120_N123_N1.4840.99
275_T337_T1.4820.99
190_G213_F1.4740.99
148_A176_C1.4690.99
49_S52_E1.4560.99
259_I364_R1.4510.99
260_D264_R1.4500.99
302_R317_T1.4170.99
347_L363_F1.4060.99
35_V345_L1.4040.99
198_E236_F1.3990.99
16_Q358_D1.3980.99
117_H126_K1.3810.99
24_V31_F1.3810.99
139_N183_Q1.3760.99
7_R25_H1.3700.99
202_T236_F1.3660.99
231_N260_D1.3500.99
50_P316_Y1.3500.99
88_N316_Y1.3460.99
283_V304_I1.3330.99
49_S334_R1.3250.99
84_H93_I1.3210.99
330_T333_L1.3180.99
76_I299_R1.3100.99
307_E370_R1.3070.99
325_I336_T1.3050.99
32_E356_R1.3050.99
60_I145_I1.2960.99
363_F367_F1.2910.99
282_T289_R1.2790.98
134_A138_Q1.2710.98
322_R326_R1.2630.98
346_H350_Q1.2500.98
258_L348_M1.2320.98
205_A240_L1.2320.98
311_Y338_Y1.2260.98
227_K257_A1.2230.98
15_K243_K1.2190.98
249_L266_V1.2070.98
117_H128_F1.2060.98
112_E128_F1.1980.98
148_A173_A1.1970.98
249_L261_G1.1950.98
286_A307_E1.1940.98
189_F268_M1.1860.97
277_I280_N1.1830.97
11_I21_L1.1760.97
139_N181_N1.1760.97
162_M197_Y1.1750.97
353_Q356_R1.1700.97
258_L351_V1.1700.97
349_E353_Q1.1690.97
151_E165_S1.1650.97
177_L211_L1.1610.97
325_I332_G1.1600.97
286_A346_H1.1530.97
88_N315_N1.1450.97
206_K210_S1.1370.97
18_G239_P1.1330.97
354_A359_R1.1310.97
208_L216_Y1.1280.97
15_K358_D1.1240.96
42_L327_C1.1160.96
135_M146_M1.1080.96
249_L252_V1.1020.96
69_W74_Q1.0990.96
308_C339_H1.0970.96
177_L181_N1.0920.96
359_R362_D1.0890.96
263_I361_G1.0880.96
233_V236_F1.0860.96
148_A169_T1.0810.96
203_Q206_K1.0800.96
26_T29_G1.0800.96
300_D318_R1.0790.96
342_H349_E1.0770.95
142_G186_Q1.0730.95
286_A370_R1.0670.95
160_D164_R1.0620.95
160_D163_K1.0600.95
174_E210_S1.0600.95
83_L141_L1.0580.95
212_D245_K1.0580.95
16_Q355_I1.0570.95
134_A149_F1.0540.95
313_C339_H1.0510.95
133_K137_I1.0490.95
205_A241_L1.0460.95
161_Y165_S1.0450.95
139_N180_H1.0440.95
312_T321_I1.0390.95
111_I149_F1.0380.94
48_M341_L1.0370.94
273_L284_F1.0350.94
136_E140_A1.0340.94
198_E202_T1.0310.94
155_Y226_P1.0280.94
135_M176_C1.0240.94
43_A63_S1.0190.94
35_V268_M1.0030.93
129_L134_A1.0010.93
69_W129_L1.0010.93
292_M325_I0.9990.93
159_Y197_Y0.9950.93
26_T31_F0.9950.93
285_T288_G0.9950.93
234_L261_G0.9900.93
157_A195_G0.9780.93
93_I143_S0.9780.93
53_L56_M0.9780.93
80_A319_A0.9770.92
260_D368_F0.9740.92
177_L214_P0.9740.92
58_A345_L0.9730.92
159_Y162_M0.9700.92
170_S208_L0.9680.92
153_P166_V0.9680.92
166_V170_S0.9670.92
177_L213_F0.9660.92
298_E322_R0.9610.92
112_E117_H0.9590.92
286_A305_D0.9570.92
76_I79_E0.9520.91
173_A211_L0.9510.91
293_K325_I0.9370.91
273_L344_L0.9340.91
290_L297_F0.9330.91
155_Y196_E0.9210.90
237_T240_L0.9200.90
278_A338_Y0.9190.90
209_I216_Y0.9150.90
138_Q143_S0.9130.90
87_M93_I0.9120.90
156_P195_G0.9100.90
259_I367_F0.9080.89
135_M149_F0.9070.89
66_Y121_H0.9020.89
49_S331_F0.9000.89
48_M338_Y0.8980.89
147_M191_I0.8940.89
30_S59_G0.8910.89
283_V321_I0.8900.88
312_T315_N0.8900.88
109_R151_E0.8900.88
59_G92_A0.8880.88
254_S273_L0.8840.88
204_S208_L0.8840.88
351_V354_A0.8810.88
45_V53_L0.8800.88
365_E369_E0.8790.88
309_D349_E0.8770.88
161_Y164_R0.8760.88
198_E233_V0.8550.86
63_S83_L0.8530.86
114_E128_F0.8510.86
206_K240_L0.8480.86
204_S207_D0.8410.86
290_L318_R0.8400.85
284_F344_L0.8400.85
16_Q361_G0.8360.85
263_I368_F0.8360.85
70_L122_L0.8360.85
256_D368_F0.8320.85
56_M345_L0.8310.85
300_D319_A0.8300.85
174_E207_D0.8250.84
359_R366_E0.8250.84
362_D366_E0.8230.84
85_K88_N0.8220.84
354_A360_L0.8220.84
207_D211_L0.8210.84
260_D364_R0.8210.84
286_A343_F0.8180.84
235_E238_T0.8170.84
102_V149_F0.8140.84
228_D232_R0.8140.84
187_G214_P0.8090.83
360_L364_R0.8080.83
192_V205_A0.8050.83
61_I87_M0.8040.83
182_R185_E0.8000.83
259_I363_F0.7960.82
85_K90_D0.7960.82
59_G91_R0.7960.82
218_I221_L0.7940.82
324_L331_F0.7920.82
227_K230_M0.7850.81
323_H329_E0.7840.81
192_V208_L0.7830.81
14_C358_D0.7820.81
166_V203_Q0.7820.81
301_F317_T0.7810.81
147_M250_M0.7800.81
301_F319_A0.7790.81
10_F21_L0.7780.81
166_V204_S0.7780.81
102_V116_V0.7760.81
343_F347_L0.7750.81
281_G325_I0.7730.80
292_M336_T0.7720.80
170_S211_L0.7670.80
205_A209_I0.7660.80
31_F35_V0.7640.80
258_L261_G0.7630.79
311_Y316_Y0.7610.79
48_M53_L0.7600.79
237_T241_L0.7600.79
361_G365_E0.7580.79
272_V276_R0.7580.79
76_I80_A0.7560.79
117_H130_S0.7550.79
277_I282_T0.7540.79
137_I141_L0.7510.78
320_Y323_H0.7500.78
135_M148_A0.7490.78
35_V60_I0.7490.78
351_V363_F0.7470.78
69_W133_K0.7440.78
81_G85_K0.7440.78
157_A162_M0.7420.78
283_V325_I0.7400.77
221_L225_E0.7400.77
48_M337_T0.7390.77
107_K119_R0.7350.77
135_M139_N0.7350.77
151_E168_R0.7290.76
196_E222_S0.7270.76
164_R168_R0.7240.76
50_P311_Y0.7230.76
277_I284_F0.7230.76
162_M200_L0.7200.75
310_C335_L0.7200.75
47_T334_R0.7190.75
238_T264_R0.7190.75
196_E229_V0.7180.75
170_S210_S0.7140.75
321_I325_I0.7130.75
50_P87_M0.7120.75
312_T316_Y0.7120.75
16_Q362_D0.7100.74
211_L214_P0.7090.74
196_E233_V0.7090.74
321_I336_T0.7040.74
328_N333_L0.7040.74
57_D349_E0.7030.74
313_C335_L0.7020.74
178_N182_R0.7010.73
51_E88_N0.6990.73
308_C335_L0.6970.73
116_V149_F0.6950.73
48_M334_R0.6950.73
74_Q136_E0.6940.73
177_L212_D0.6940.73
304_I336_T0.6940.73
164_R167_E0.6930.73
310_C339_H0.6920.73
21_L351_V0.6900.72
334_R338_Y0.6900.72
202_T239_P0.6890.72
177_L216_Y0.6870.72
11_I356_R0.6870.72
17_T361_G0.6860.72
332_G336_T0.6860.72
281_G292_M0.6830.72
205_A238_T0.6830.72
71_R122_L0.6820.71
7_R30_S0.6780.71
128_F133_K0.6770.71
254_S344_L0.6720.70
308_C315_N0.6710.70
26_T92_A0.6700.70
22_G33_T0.6690.70
57_D91_R0.6670.70
156_P196_E0.6670.70
18_G235_E0.6640.69
37_M60_I0.6630.69
236_F240_L0.6620.69
153_P196_E0.6600.69
234_L238_T0.6600.69
273_L277_I0.6590.69
21_L360_L0.6580.69
135_M188_L0.6570.69
42_L71_R0.6560.68
354_A363_F0.6540.68
23_K59_G0.6540.68
364_R368_F0.6530.68
350_Q353_Q0.6520.68
139_N146_M0.6520.68
259_I360_L0.6510.68
14_C361_G0.6510.68
181_N184_D0.6500.68
277_I283_V0.6440.67
192_V237_T0.6440.67
9_E32_E0.6430.67
254_S257_A0.6430.67
238_T247_R0.6420.67
325_I333_L0.6400.67
216_Y245_K0.6400.67
229_V233_V0.6400.67
153_P165_S0.6360.66
136_E182_R0.6280.65
291_N294_N0.6270.65
147_M248_Y0.6260.65
246_P268_M0.6240.65
26_T186_Q0.6240.65
308_C342_H0.6230.64
222_S225_E0.6220.64
27_P187_G0.6200.64
320_Y324_L0.6200.64
218_I237_T0.6190.64
47_T333_L0.6180.64
277_I336_T0.6180.64
74_Q77_V0.6180.64
134_A176_C0.6150.64
40_G45_V0.6130.63
259_I347_L0.6130.63
177_L210_S0.6110.63
36_F274_P0.6100.63
80_A317_T0.6090.63
9_E30_S0.6080.63
155_Y195_G0.6070.62
261_G269_F0.6060.62
146_M180_H0.6060.62
292_M321_I0.6040.62
129_L133_K0.6030.62
338_Y342_H0.6020.62
113_E168_R0.6010.62
290_L302_R0.6010.62
295_A322_R0.6010.62
231_N257_A0.6000.62
69_W137_I0.6000.62
312_T342_H0.5970.61
202_T205_A0.5960.61
76_I298_E0.5950.61
94_L147_M0.5940.61
281_G293_K0.5930.61
280_N330_T0.5930.61
286_A304_I0.5920.61
143_S148_A0.5910.60
43_A83_L0.5890.60
156_P226_P0.5890.60
53_L57_D0.5890.60
77_V299_R0.5880.60
233_V237_T0.5870.60
165_S168_R0.5860.60
28_H82_G0.5860.60
51_E315_N0.5840.60
35_V59_G0.5830.59
179_A183_Q0.5800.59
74_Q137_I0.5790.59
55_A314_K0.5790.59
283_V290_L0.5780.59
167_E171_R0.5770.59
208_L213_F0.5740.58
191_I250_M0.5720.58
320_Y329_E0.5720.58
308_C312_T0.5710.58
69_W125_D0.5690.58
111_I161_Y0.5680.57
287_E307_E0.5650.57
225_E248_Y0.5650.57
31_F59_G0.5640.57
256_D367_F0.5640.57
287_E306_E0.5620.57
262_A269_F0.5610.57
238_T241_L0.5610.57
18_G243_K0.5600.56
337_T344_L0.5600.56
146_M186_Q0.5600.56
56_M342_H0.5590.56
259_I262_A0.5590.56
259_I368_F0.5580.56
259_I343_F0.5580.56
37_M94_L0.5580.56
276_R279_R0.5580.56
148_A213_F0.5570.56
232_R236_F0.5550.56
162_M166_V0.5540.56
348_M351_V0.5520.55
218_I238_T0.5510.55
227_K231_N0.5500.55
350_Q354_A0.5490.55
37_M147_M0.5490.55
235_E239_P0.5480.55
129_L137_I0.5450.54
345_L349_E0.5450.54
227_K256_D0.5450.54
47_T278_A0.5450.54
159_Y200_L0.5420.54
170_S209_I0.5410.54
61_I93_I0.5400.54
146_M149_F0.5400.54
139_N179_A0.5390.54
82_G85_K0.5380.53
168_R171_R0.5380.53
219_G225_E0.5380.53
50_P88_N0.5370.53
79_E301_F0.5370.53
292_M332_G0.5360.53
31_F92_A0.5360.53
180_H186_Q0.5350.53
180_H214_P0.5340.53
79_E319_A0.5330.53
53_L58_A0.5330.53
282_T291_N0.5330.53
304_I312_T0.5320.53
313_C342_H0.5310.52
258_L344_L0.5310.52
208_L211_L0.5300.52
305_D346_H0.5300.52
354_A358_D0.5290.52
64_N68_L0.5280.52
310_C342_H0.5280.52
256_D289_R0.5270.52
51_E56_M0.5260.52
280_N325_I0.5260.52
277_I344_L0.5250.52
27_P180_H0.5250.52
103_F222_S0.5240.52
198_E240_L0.5240.52
335_L339_H0.5240.52
28_H186_Q0.5230.51
194_G201_R0.5230.51
108_F121_H0.5220.51
44_T331_F0.5220.51
109_R165_S0.5220.51
227_K278_A0.5210.51
177_L180_H0.5210.51
50_P312_T0.5210.51
343_F368_F0.5200.51
293_K332_G0.5180.51
113_E175_R0.5150.50
110_N117_H0.5150.50
94_L250_M0.5140.50
60_I94_L0.5140.50
96_D150_D0.5130.50
113_E165_S0.5110.50
106_S109_R0.5100.50
231_N264_R0.5100.50
32_E349_E0.5100.50
77_V80_A0.5100.50
212_D216_Y0.5100.50
238_T266_V0.5100.50
48_M330_T0.5090.50
354_A357_E0.5070.49
166_V169_T0.5060.49
20_R247_R0.5060.49
209_I240_L0.5060.49
155_Y162_M0.5060.49
213_F216_Y0.5040.49
228_D237_T0.5020.49
203_Q210_S0.5020.49
361_G364_R0.5010.48
14_C356_R0.5010.48
51_E334_R0.5000.48
122_L327_C0.5000.48
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4q8wA 2 0.9606 100 0.069 Contact Map
2ashA 3 0.9475 100 0.089 Contact Map
1iq8A 2 0.895 100 0.203 Contact Map
1ydnA 1 0.6798 89.6 0.956 Contact Map
3oixA 3 0.7087 87.2 0.958 Contact Map
3t7vA 1 0.7139 86.1 0.959 Contact Map
4xp7A 1 0.7979 85 0.959 Contact Map
4oqvA 1 0.7533 83.1 0.96 Contact Map
1ydoA 3 0.685 82.6 0.96 Contact Map
1vcfA 3 0.7218 82.1 0.96 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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