YORG - BACSU - GREMLIN Contacts

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YORG - SPBc2 prophage-derived uncharacterized protein YorG

UniProt:	O31907	Sequences: MAIDIFNPQVSVVAKGLEGKVITIYGSNNLGKTKQSTRMKKPLYLPFEKGLNAIAGVQFMPINSWADFKKVNKQLTKNAEKAKEMYQTIIVDEVDAFAKYATRYVCEQYDVERIKDGNDGFGLWKEYETEVWEEINKLIGVGFTVIFIAHAAEDKKGKVYPKGDKRVLAPVIDNSDIVLYLSSNGVDEDRKVIKSSAWLAETEEHFARSRFDYIDTYLPEFTAENLEKAIIEAVERQEQAEGIVAVTYEEQKQNNASEELDFNSLMDQIKEIGMKLNEEGRLEEVNEITEKHLGKGVKVTECSRKQVGVMSVILDDLKDLLAE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	323 (286)
Sequences:	206 (181)
Seq/√Len:	10.7

BACSU - YORG

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
41_K	87_Q	5.296	1.00
29_N	208_R	5.031	1.00
45_L	89_I	4.242	0.99
20_K	53_A	3.990	0.99
101_A	131_V	3.946	0.99
94_V	166_R	3.575	0.98
94_V	171_V	3.119	0.95
113_R	126_E	3.015	0.94
53_A	211_F	2.935	0.93
35_Q	181_L	2.808	0.92
111_V	117_G	2.756	0.91
13_V	57_V	2.645	0.89
149_A	214_I	2.578	0.87
58_Q	86_Y	2.387	0.83
33_T	54_I	2.269	0.79
42_P	88_T	2.262	0.79
65_W	101_A	2.180	0.77
147_F	171_V	2.174	0.76
162_K	173_D	2.153	0.76
73_K	137_K	2.117	0.74
47_F	50_G	2.105	0.74
298_K	301_E	2.031	0.71
115_K	124_W	1.961	0.68
24_I	35_Q	1.923	0.67
18_E	52_N	1.916	0.66
134_E	164_D	1.909	0.66
134_E	187_D	1.904	0.66
121_F	134_E	1.865	0.64
235_E	250_E	1.841	0.63
35_Q	221_F	1.831	0.63
42_P	90_I	1.818	0.62
34_K	221_F	1.799	0.61
22_I	147_F	1.757	0.59
243_I	250_E	1.715	0.58
17_L	53_A	1.714	0.57
101_A	154_D	1.692	0.56
32_K	92_D	1.689	0.56
29_N	46_P	1.624	0.53
102_T	127_Y	1.624	0.53
78_N	84_E	1.611	0.53
24_I	194_K	1.605	0.52
118_N	160_Y	1.574	0.51
101_A	204_E	1.566	0.51
290_E	295_K	1.538	0.49
173_D	180_Y	1.516	0.48
105_V	127_Y	1.511	0.48
34_K	181_L	1.510	0.48
264_S	272_I	1.508	0.48
290_E	296_G	1.505	0.48
287_E	299_V	1.498	0.47
93_E	150_H	1.490	0.47
90_I	300_T	1.489	0.47
94_V	164_D	1.485	0.47
281_R	284_E	1.474	0.46
36_S	42_P	1.470	0.46
24_I	72_N	1.458	0.45
16_G	69_K	1.450	0.45
182_S	198_W	1.413	0.43
28_N	174_N	1.409	0.43
121_F	169_A	1.409	0.43
68_F	163_G	1.404	0.43
204_E	230_I	1.368	0.41
26_G	155_K	1.358	0.41
235_E	265_L	1.358	0.41
121_F	165_K	1.354	0.41
47_F	97_F	1.353	0.41
21_V	34_K	1.350	0.40
99_K	102_T	1.350	0.40
96_A	173_D	1.349	0.40
94_V	106_C	1.338	0.40
154_D	178_V	1.336	0.40
197_A	221_F	1.324	0.39
34_K	119_D	1.311	0.39
251_Q	277_N	1.294	0.38
43_L	284_E	1.292	0.38
150_H	155_K	1.291	0.38
118_N	122_G	1.288	0.38
245_A	268_Q	1.278	0.37
279_E	284_E	1.270	0.37
248_Y	258_E	1.257	0.36
28_N	162_K	1.249	0.36
292_H	301_E	1.247	0.36
85_M	165_K	1.227	0.35
284_E	298_K	1.226	0.35
29_N	93_E	1.224	0.35
79_A	85_M	1.221	0.35
20_K	211_F	1.209	0.34
40_K	88_T	1.209	0.34
36_S	90_I	1.207	0.34
95_D	114_I	1.204	0.34
288_I	298_K	1.203	0.34
147_F	161_P	1.198	0.34
91_V	145_V	1.182	0.33
277_N	281_R	1.176	0.33
184_N	217_Y	1.174	0.33
75_L	86_Y	1.171	0.33
117_G	122_G	1.160	0.32
36_S	88_T	1.156	0.32
251_Q	264_S	1.151	0.32
263_N	274_M	1.145	0.31
232_E	260_L	1.135	0.31
74_Q	86_Y	1.126	0.31
269_I	287_E	1.125	0.31
108_Q	112_E	1.125	0.31
115_K	122_G	1.124	0.31
227_E	230_I	1.119	0.30
197_A	218_L	1.118	0.30
294_G	298_K	1.118	0.30
55_A	171_V	1.114	0.30
69_K	104_Y	1.114	0.30
287_E	294_G	1.110	0.30
88_T	146_I	1.103	0.30
71_V	75_L	1.101	0.30
235_E	249_E	1.095	0.29
247_T	261_D	1.090	0.29
273_G	282_L	1.088	0.29
292_H	299_V	1.087	0.29
154_D	206_F	1.083	0.29
52_N	70_K	1.083	0.29
245_A	261_D	1.083	0.29
127_Y	130_E	1.082	0.29
106_C	113_R	1.078	0.29
249_E	258_E	1.076	0.29
165_K	169_A	1.075	0.29
95_D	206_F	1.073	0.29
61_P	264_S	1.070	0.29
24_I	73_K	1.067	0.28
114_I	210_R	1.065	0.28
16_G	21_V	1.063	0.28
244_V	264_S	1.063	0.28
147_F	166_R	1.058	0.28
290_E	293_L	1.054	0.28
260_L	263_N	1.053	0.28
44_Y	51_L	1.049	0.28
249_E	276_L	1.048	0.28
268_Q	272_I	1.043	0.27
39_M	88_T	1.040	0.27
10_V	60_M	1.039	0.27
31_G	92_D	1.038	0.27
177_I	193_I	1.037	0.27
31_G	195_S	1.028	0.27
275_K	281_R	1.019	0.27
186_V	191_K	1.016	0.26
37_T	221_F	1.013	0.26
35_Q	303_S	1.013	0.26
74_Q	218_L	1.010	0.26
235_E	263_N	1.009	0.26
29_N	150_H	1.008	0.26
46_P	209_S	1.008	0.26
279_E	286_N	1.007	0.26
111_V	122_G	0.999	0.26
255_N	268_Q	0.998	0.26
286_N	298_K	0.993	0.26
242_G	249_E	0.993	0.26
159_V	203_E	0.993	0.26
264_S	276_L	0.992	0.26
282_L	287_E	0.988	0.25
245_A	259_E	0.983	0.25
35_Q	236_R	0.980	0.25
278_E	281_R	0.976	0.25
268_Q	291_K	0.973	0.25
242_G	250_E	0.972	0.25
275_K	283_E	0.965	0.25
46_P	208_R	0.965	0.25
250_E	253_Q	0.958	0.24
19_G	54_I	0.958	0.24
111_V	128_E	0.954	0.24
63_N	273_G	0.950	0.24
24_I	31_G	0.946	0.24
140_G	287_E	0.945	0.24
18_E	70_K	0.945	0.24
279_E	285_V	0.942	0.24
65_W	131_V	0.937	0.24
248_Y	275_K	0.935	0.24
21_V	144_T	0.934	0.24
257_S	260_L	0.934	0.24
181_L	221_F	0.932	0.23
280_G	287_E	0.928	0.23
126_E	169_A	0.927	0.23
29_N	144_T	0.926	0.23
70_K	73_K	0.925	0.23
117_G	184_N	0.924	0.23
245_A	260_L	0.917	0.23
33_T	267_D	0.916	0.23
233_A	260_L	0.913	0.23
244_V	247_T	0.910	0.23
236_R	268_Q	0.906	0.23
280_G	284_E	0.894	0.22
107_E	244_V	0.894	0.22
147_F	150_H	0.892	0.22
289_T	294_G	0.889	0.22
236_R	288_I	0.886	0.22
23_T	102_T	0.885	0.22
65_W	197_A	0.883	0.22
154_D	160_Y	0.883	0.22
248_Y	266_M	0.880	0.22
70_K	266_M	0.879	0.22
121_F	171_V	0.878	0.22
274_M	292_H	0.875	0.22
104_Y	284_E	0.874	0.22
33_T	67_D	0.872	0.21
288_I	294_G	0.872	0.21
120_G	179_L	0.865	0.21
91_V	164_D	0.865	0.21
246_V	269_I	0.863	0.21
128_E	164_D	0.852	0.21
16_G	33_T	0.851	0.21
34_K	197_A	0.850	0.21
54_I	150_H	0.850	0.21
63_N	136_N	0.849	0.21
95_D	172_I	0.847	0.21
24_I	292_H	0.845	0.21
273_G	279_E	0.839	0.20
154_D	221_F	0.838	0.20
34_K	150_H	0.837	0.20
209_S	214_I	0.835	0.20
49_K	168_L	0.833	0.20
242_G	270_K	0.832	0.20
264_S	267_D	0.831	0.20
88_T	150_H	0.829	0.20
180_Y	207_A	0.829	0.20
53_A	74_Q	0.828	0.20
13_V	33_T	0.828	0.20
247_T	274_M	0.828	0.20
36_S	40_K	0.821	0.20
40_K	155_K	0.821	0.20
255_N	284_E	0.820	0.20
24_I	304_R	0.819	0.20
45_L	293_L	0.818	0.20
24_I	245_A	0.814	0.20
45_L	157_G	0.814	0.20
31_G	122_G	0.811	0.20
194_K	258_E	0.808	0.20
86_Y	169_A	0.802	0.19
28_N	150_H	0.796	0.19
286_N	304_R	0.795	0.19
162_K	180_Y	0.790	0.19
106_C	115_K	0.783	0.19
106_C	154_D	0.781	0.19
260_L	266_M	0.771	0.18
86_Y	165_K	0.770	0.18
254_N	272_I	0.769	0.18
252_K	271_E	0.768	0.18
48_E	129_T	0.764	0.18
34_K	142_G	0.761	0.18
62_I	68_F	0.757	0.18
10_V	77_K	0.755	0.18
20_K	231_I	0.754	0.18
29_N	36_S	0.753	0.18
43_L	71_V	0.753	0.18
75_L	143_F	0.752	0.18
29_N	88_T	0.748	0.18
28_N	173_D	0.747	0.18
117_G	229_A	0.747	0.18
284_E	287_E	0.743	0.18
35_Q	50_G	0.743	0.18
261_D	287_E	0.739	0.18
253_Q	286_N	0.738	0.18
174_N	180_Y	0.737	0.17
279_E	282_L	0.737	0.17
164_D	187_D	0.736	0.17
264_S	281_R	0.735	0.17
58_Q	132_W	0.735	0.17
290_E	297_V	0.733	0.17
50_G	96_A	0.733	0.17
262_F	266_M	0.732	0.17
264_S	269_I	0.731	0.17
91_V	159_V	0.730	0.17
285_V	288_I	0.726	0.17
26_G	32_K	0.725	0.17
151_A	180_Y	0.722	0.17
257_S	261_D	0.722	0.17
85_M	225_N	0.721	0.17
134_E	208_R	0.719	0.17
13_V	159_V	0.718	0.17
285_V	298_K	0.716	0.17
179_L	199_L	0.716	0.17
34_K	37_T	0.715	0.17
93_E	208_R	0.714	0.17
42_P	134_E	0.710	0.17
253_Q	276_L	0.710	0.17
94_V	110_D	0.709	0.17
153_E	187_D	0.706	0.17
141_V	233_A	0.704	0.17
49_K	96_A	0.697	0.16
220_E	304_R	0.696	0.16
65_W	97_F	0.692	0.16
289_T	297_V	0.690	0.16
268_Q	286_N	0.690	0.16
271_E	287_E	0.688	0.16
66_A	171_V	0.687	0.16
108_Q	261_D	0.682	0.16
262_F	270_K	0.681	0.16
44_Y	174_N	0.681	0.16
251_Q	263_N	0.681	0.16
259_E	272_I	0.680	0.16
246_V	279_E	0.679	0.16
136_N	149_A	0.677	0.16
271_E	280_G	0.675	0.16
50_G	149_A	0.664	0.16
272_I	279_E	0.664	0.16
297_V	302_C	0.660	0.15
136_N	207_A	0.659	0.15
253_Q	268_Q	0.658	0.15
141_V	171_V	0.654	0.15
33_T	51_L	0.653	0.15
256_A	266_M	0.652	0.15
282_L	285_V	0.650	0.15
69_K	130_E	0.647	0.15
265_L	278_E	0.645	0.15
293_L	296_G	0.645	0.15
45_L	71_V	0.644	0.15
26_G	174_N	0.643	0.15
37_T	44_Y	0.640	0.15
241_E	248_Y	0.639	0.15
143_F	208_R	0.638	0.15
125_K	179_L	0.636	0.15
132_W	136_N	0.636	0.15
46_P	88_T	0.633	0.15
120_G	199_L	0.632	0.15
40_K	144_T	0.631	0.15
50_G	67_D	0.631	0.15
236_R	248_Y	0.626	0.15
257_S	286_N	0.624	0.15
269_I	274_M	0.618	0.14
16_G	41_K	0.613	0.14
255_N	259_E	0.612	0.14
11_S	48_E	0.611	0.14
122_G	142_G	0.610	0.14
172_I	206_F	0.610	0.14
70_K	89_I	0.610	0.14
181_L	197_A	0.609	0.14
294_G	299_V	0.606	0.14
21_V	51_L	0.606	0.14
89_I	243_I	0.605	0.14
42_P	226_L	0.605	0.14
33_T	141_V	0.605	0.14
85_M	141_V	0.602	0.14
266_M	275_K	0.601	0.14
50_G	107_E	0.601	0.14
172_I	178_V	0.600	0.14
269_I	275_K	0.600	0.14
117_G	206_F	0.599	0.14
55_A	209_S	0.596	0.14
48_E	161_P	0.593	0.14
66_A	259_E	0.592	0.14
105_V	125_K	0.590	0.14
283_E	298_K	0.589	0.14
127_Y	164_D	0.588	0.14
73_K	257_S	0.587	0.14
120_G	176_D	0.587	0.14
194_K	220_E	0.586	0.14
180_Y	205_H	0.585	0.14
48_E	95_D	0.584	0.14
114_I	129_T	0.584	0.14
144_T	208_R	0.584	0.14
128_E	167_V	0.584	0.14
258_E	271_E	0.583	0.14
12_V	60_M	0.583	0.14
78_N	218_L	0.581	0.14
153_E	165_K	0.581	0.14
232_E	270_K	0.580	0.14
225_N	244_V	0.578	0.14
293_L	297_V	0.577	0.14
97_F	197_A	0.576	0.14
12_V	70_K	0.575	0.13
200_A	216_T	0.573	0.13
11_S	129_T	0.571	0.13
27_S	55_A	0.570	0.13
46_P	113_R	0.570	0.13
254_N	268_Q	0.569	0.13
233_A	262_F	0.569	0.13
116_D	124_W	0.566	0.13
21_V	150_H	0.563	0.13
225_N	254_N	0.562	0.13
17_L	54_I	0.561	0.13
102_T	111_V	0.558	0.13
235_E	241_E	0.558	0.13
281_R	288_I	0.557	0.13
11_S	14_A	0.556	0.13
227_E	231_I	0.556	0.13
28_N	184_N	0.555	0.13
24_I	266_M	0.551	0.13
99_K	104_Y	0.551	0.13
113_R	152_A	0.551	0.13
170_P	227_E	0.550	0.13
232_E	242_G	0.550	0.13
25_Y	29_N	0.548	0.13
281_R	286_N	0.547	0.13
102_T	109_Y	0.546	0.13
74_Q	108_Q	0.544	0.13
271_E	299_V	0.541	0.13
261_D	265_L	0.540	0.13
252_K	255_N	0.540	0.13
164_D	167_V	0.539	0.13
250_E	280_G	0.538	0.13
66_A	232_E	0.537	0.13
283_E	291_K	0.534	0.13
150_H	176_D	0.533	0.13
10_V	85_M	0.533	0.13
118_N	129_T	0.533	0.13
51_L	174_N	0.532	0.13
19_G	136_N	0.531	0.13
296_G	302_C	0.531	0.13
121_F	164_D	0.530	0.13
116_D	167_V	0.529	0.12
56_G	160_Y	0.529	0.12
105_V	267_D	0.528	0.12
249_E	281_R	0.528	0.12
253_Q	257_S	0.527	0.12
121_F	128_E	0.525	0.12
170_P	204_E	0.524	0.12
247_T	265_L	0.524	0.12
105_V	122_G	0.524	0.12
18_E	171_V	0.523	0.12
153_E	160_Y	0.522	0.12
222_T	259_E	0.518	0.12
99_K	225_N	0.517	0.12
251_Q	266_M	0.516	0.12
174_N	182_S	0.515	0.12
98_A	143_F	0.515	0.12
90_I	150_H	0.514	0.12
254_N	264_S	0.510	0.12
95_D	118_N	0.507	0.12
158_K	193_I	0.504	0.12
272_I	276_L	0.504	0.12
45_L	62_I	0.502	0.12
34_K	93_E	0.502	0.12
274_M	291_K	0.500	0.12

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4ppfA	1	0.7709	98.9	0.866	Contact Map
3hr8A	1	0.8607	98.7	0.877	Contact Map
1n0wA	1	0.5387	98.6	0.878	Contact Map
3ldaA	1	0.5573	98.6	0.879	Contact Map
2cvhA	1	0.6068	98.6	0.88	Contact Map
2q6tA	1	0.6285	98.6	0.88	Contact Map
1u94A	3	0.7585	98.5	0.881	Contact Map
2z43A	1	0.5511	98.5	0.884	Contact Map
3bgwA	3	0.644	98.4	0.885	Contact Map
1pznA	5	0.5697	98.4	0.885	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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