YORJ - BACSU - GREMLIN Contacts

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YORJ - SPBc2 prophage-derived uncharacterized protein YorJ

UniProt:	O31904	Sequences: MKYDKDRVKESLTIEDIHKILKDLGSENNLWDQQGNPIYRTVCHNASGGSYKLYYYHEAKQFHCYTECGDNFDVFELVIRAKSQKGINISFNQAIEYVAKIAGRTFGFGNRETYINNDLIDDWEWMGKFKKKKKIHIELPSFNETVLDVFVPYPHQLWLGEGISHKTLKEFEIGYYFRPHTEGITIPHRDLNNRLIGIRRRSMIKEEVDAGYKYMPLKVGNILYNHQTMMNLYGLHKTKNSIERFKKVLIFESEKSVLKCQDFYGESNFTCAVCSSNISNFHRDILLSLGVEEVFIALDKYRPPKEHETEEKYQEKLVEYQKKILKLAAKFTPYVRVYVLWDYEGLLDYKDSPADKGKETLEELMRRKIEIGTDEGGI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	378 (333)
Sequences:	3906 (2676)
Seq/√Len:	146.6

ECOLI - DNAG
BACSU - YORJ DNAG

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
70_D	80_R	4.433	1.00
143_N	146_V	3.230	1.00
329_A	333_P	3.140	1.00
190_D	194_R	3.022	1.00
33_Q	38_I	2.979	1.00
26_S	101_I	2.924	1.00
199_R	254_E	2.777	1.00
196_I	231_N	2.717	1.00
14_I	17_I	2.653	1.00
41_T	68_C	2.557	1.00
38_I	54_Y	2.522	1.00
159_L	165_H	2.483	1.00
152_P	156_Q	2.468	1.00
143_N	147_L	2.410	1.00
167_T	262_D	2.405	1.00
351_D	359_E	2.358	1.00
62_F	80_R	2.349	1.00
48_G	66_T	2.331	1.00
18_H	102_A	2.328	1.00
329_A	337_V	2.298	1.00
273_V	298_L	2.159	1.00
35_G	58_E	2.136	1.00
127_G	130_K	2.124	1.00
158_W	184_I	2.088	1.00
89_I	97_Y	2.086	1.00
44_H	68_C	2.078	1.00
82_K	97_Y	2.053	1.00
63_H	69_G	2.045	1.00
21_L	37_P	2.045	1.00
189_R	236_H	2.036	1.00
247_K	269_F	2.016	1.00
124_E	130_K	2.009	1.00
90_S	93_Q	2.007	1.00
302_R	306_E	2.007	1.00
41_T	44_H	1.973	1.00
18_H	23_D	1.944	1.00
156_Q	160_G	1.937	1.00
96_E	108_F	1.931	1.00
248_V	286_L	1.929	1.00
292_E	321_Q	1.880	1.00
230_M	281_F	1.827	1.00
242_I	248_V	1.814	1.00
48_G	53_L	1.807	1.00
36_N	54_Y	1.790	1.00
30_L	55_Y	1.779	1.00
163_I	255_K	1.767	1.00
241_S	268_N	1.751	1.00
11_S	107_G	1.741	1.00
197_G	217_L	1.736	1.00
199_R	274_C	1.733	1.00
76_E	80_R	1.724	1.00
340_L	344_E	1.707	1.00
126_M	130_K	1.698	1.00
147_L	151_V	1.697	1.00
170_E	268_N	1.695	1.00
27_E	82_K	1.686	1.00
189_R	237_K	1.682	1.00
172_E	189_R	1.671	1.00
28_N	39_Y	1.666	1.00
63_H	71_N	1.666	1.00
334_Y	338_Y	1.659	1.00
226_H	274_C	1.650	1.00
15_E	73_D	1.638	1.00
127_G	225_N	1.620	1.00
73_D	76_E	1.613	1.00
165_H	168_L	1.609	1.00
64_C	68_C	1.605	1.00
202_S	206_E	1.599	1.00
78_V	97_Y	1.597	1.00
19_K	57_H	1.588	1.00
165_H	169_K	1.572	1.00
295_F	325_L	1.567	1.00
123_W	130_K	1.565	1.00
124_E	127_G	1.554	1.00
27_E	81_A	1.540	1.00
154_P	184_I	1.540	1.00
39_Y	55_Y	1.524	1.00
23_D	74_V	1.516	1.00
292_E	323_K	1.491	1.00
251_F	270_T	1.491	1.00
56_Y	59_A	1.490	1.00
164_S	262_D	1.489	1.00
186_I	233_Y	1.485	1.00
41_T	64_C	1.479	1.00
36_N	56_Y	1.468	1.00
62_F	70_D	1.465	1.00
217_L	223_L	1.465	1.00
164_S	167_T	1.456	1.00
41_T	66_T	1.454	1.00
44_H	66_T	1.444	1.00
91_F	95_I	1.435	1.00
344_E	348_D	1.427	1.00
247_K	292_E	1.404	1.00
300_K	329_A	1.382	1.00
168_L	173_I	1.379	1.00
345_G	349_Y	1.377	1.00
56_Y	61_Q	1.372	1.00
13_T	16_D	1.370	1.00
338_Y	346_L	1.366	1.00
229_M	281_F	1.361	1.00
21_L	57_H	1.359	1.00
153_Y	182_E	1.356	1.00
222_I	227_Q	1.353	1.00
15_E	74_V	1.336	1.00
89_I	94_A	1.320	1.00
339_V	342_D	1.315	1.00
123_W	225_N	1.297	1.00
212_Y	215_M	1.296	1.00
283_R	314_Q	1.294	1.00
273_V	282_H	1.291	1.00
234_G	261_Q	1.286	1.00
15_E	75_F	1.285	1.00
170_E	237_K	1.280	1.00
11_S	104_R	1.251	1.00
327_L	346_L	1.247	1.00
19_K	22_K	1.242	1.00
297_A	343_Y	1.240	1.00
251_F	260_C	1.237	1.00
131_K	195_L	1.235	1.00
297_A	327_L	1.233	1.00
163_I	168_L	1.232	1.00
306_E	310_E	1.227	1.00
204_I	207_E	1.225	1.00
332_T	336_R	1.216	1.00
155_H	165_H	1.215	1.00
123_W	127_G	1.209	1.00
227_Q	230_M	1.204	1.00
283_R	315_E	1.194	1.00
50_S	66_T	1.190	1.00
323_K	350_K	1.187	1.00
254_E	274_C	1.185	1.00
30_L	39_Y	1.184	1.00
143_N	151_V	1.178	1.00
158_W	168_L	1.177	1.00
155_H	168_L	1.161	1.00
234_G	257_V	1.154	1.00
325_L	346_L	1.147	1.00
122_D	125_W	1.146	1.00
92_N	96_E	1.141	0.99
218_K	222_I	1.129	0.99
171_F	261_Q	1.129	0.99
158_W	173_I	1.127	0.99
98_V	101_I	1.127	0.99
312_K	353_P	1.121	0.99
234_G	237_K	1.120	0.99
148_D	151_V	1.118	0.99
131_K	172_E	1.118	0.99
96_E	100_K	1.114	0.99
155_H	159_L	1.113	0.99
287_L	320_Y	1.112	0.99
309_T	357_G	1.111	0.99
27_E	77_L	1.102	0.99
44_H	64_C	1.098	0.99
97_Y	101_I	1.095	0.99
40_R	48_G	1.091	0.99
345_G	348_D	1.079	0.99
48_G	52_K	1.074	0.99
128_K	172_E	1.072	0.99
342_D	345_G	1.070	0.99
18_H	74_V	1.066	0.99
121_D	125_W	1.057	0.99
335_V	343_Y	1.054	0.99
144_E	172_E	1.051	0.99
341_W	345_G	1.036	0.99
329_A	338_Y	1.029	0.99
53_L	66_T	1.023	0.99
50_S	53_L	1.023	0.99
159_L	168_L	1.018	0.99
128_K	194_R	1.018	0.99
18_H	98_V	1.017	0.99
189_R	195_L	1.017	0.99
341_W	344_E	1.015	0.99
246_K	291_V	1.015	0.99
166_K	169_K	1.008	0.99
193_N	236_H	1.005	0.99
11_S	106_F	0.999	0.99
129_F	132_K	0.997	0.99
156_Q	159_L	0.994	0.99
12_L	95_I	0.985	0.99
175_Y	181_T	0.976	0.98
144_E	148_D	0.975	0.98
78_V	89_I	0.973	0.98
62_F	71_N	0.970	0.98
39_Y	53_L	0.970	0.98
173_I	258_L	0.968	0.98
152_P	155_H	0.966	0.98
44_H	52_K	0.964	0.98
25_G	29_N	0.963	0.98
207_E	212_Y	0.963	0.98
144_E	169_K	0.952	0.98
18_H	101_I	0.949	0.98
145_T	149_V	0.948	0.98
75_F	98_V	0.943	0.98
246_K	292_E	0.938	0.98
366_R	369_I	0.938	0.98
202_S	207_E	0.935	0.98
17_I	104_R	0.932	0.98
19_K	60_K	0.928	0.98
60_K	74_V	0.925	0.98
250_I	296_I	0.925	0.98
128_K	132_K	0.922	0.98
267_S	344_E	0.909	0.97
32_D	37_P	0.909	0.97
338_Y	342_D	0.908	0.97
229_M	282_H	0.905	0.97
27_E	97_Y	0.905	0.97
142_F	146_V	0.903	0.97
20_I	23_D	0.901	0.97
37_P	57_H	0.899	0.97
260_C	270_T	0.898	0.97
153_Y	156_Q	0.896	0.97
247_K	348_D	0.896	0.97
10_E	14_I	0.896	0.97
186_I	255_K	0.886	0.97
352_S	355_D	0.885	0.97
97_Y	100_K	0.881	0.97
124_E	131_K	0.880	0.97
76_E	79_I	0.878	0.97
36_N	61_Q	0.877	0.97
351_D	355_D	0.875	0.97
170_E	261_Q	0.873	0.97
61_Q	71_N	0.873	0.97
180_H	185_T	0.866	0.97
247_K	295_F	0.863	0.97
260_C	343_Y	0.863	0.97
247_K	290_G	0.861	0.97
18_H	22_K	0.859	0.96
291_V	320_Y	0.856	0.96
125_W	130_K	0.854	0.96
300_K	328_A	0.852	0.96
232_L	271_C	0.849	0.96
235_L	289_L	0.847	0.96
48_G	51_Y	0.845	0.96
298_L	311_E	0.843	0.96
278_I	311_E	0.841	0.96
362_E	367_R	0.841	0.96
249_L	260_C	0.837	0.96
294_V	315_E	0.837	0.96
75_F	95_I	0.837	0.96
334_Y	343_Y	0.832	0.96
242_I	290_G	0.828	0.96
63_H	70_D	0.827	0.96
49_G	66_T	0.823	0.95
188_H	199_R	0.822	0.95
45_N	48_G	0.822	0.95
123_W	129_F	0.821	0.95
146_V	149_V	0.820	0.95
202_S	205_K	0.815	0.95
168_L	184_I	0.814	0.95
126_M	129_F	0.813	0.95
125_W	128_K	0.810	0.95
99_A	106_F	0.808	0.95
128_K	131_K	0.808	0.95
124_E	194_R	0.807	0.95
229_M	279_S	0.806	0.95
315_E	322_K	0.805	0.95
162_G	336_R	0.803	0.95
24_L	55_Y	0.803	0.95
40_R	66_T	0.802	0.95
309_T	360_T	0.801	0.95
182_E	204_I	0.793	0.94
260_C	335_V	0.793	0.94
146_V	151_V	0.792	0.94
305_K	326_K	0.791	0.94
360_T	363_E	0.789	0.94
259_K	263_F	0.786	0.94
241_S	245_F	0.785	0.94
245_F	344_E	0.784	0.94
177_F	218_K	0.781	0.94
166_K	170_E	0.780	0.94
36_N	59_A	0.779	0.94
280_N	314_Q	0.778	0.94
280_N	317_L	0.777	0.94
185_T	200_R	0.775	0.94
273_V	311_E	0.774	0.94
33_Q	54_Y	0.769	0.94
93_Q	96_E	0.769	0.94
251_F	257_V	0.769	0.94
188_H	231_N	0.768	0.94
81_A	88_N	0.763	0.93
233_Y	254_E	0.761	0.93
231_N	274_C	0.758	0.93
40_R	51_Y	0.757	0.93
202_S	212_Y	0.756	0.93
273_V	296_I	0.754	0.93
287_L	318_V	0.754	0.93
176_Y	181_T	0.752	0.93
163_I	186_I	0.746	0.93
296_I	315_E	0.745	0.92
79_I	91_F	0.744	0.92
24_L	28_N	0.736	0.92
183_G	200_R	0.731	0.92
259_K	262_D	0.731	0.92
227_Q	281_F	0.730	0.92
54_Y	61_Q	0.730	0.92
278_I	283_R	0.730	0.92
263_F	340_L	0.727	0.92
333_P	336_R	0.724	0.91
143_N	149_V	0.724	0.91
287_L	291_V	0.723	0.91
120_I	124_E	0.723	0.91
249_L	267_S	0.723	0.91
313_Y	357_G	0.722	0.91
286_L	320_Y	0.721	0.91
305_K	360_T	0.717	0.91
11_S	14_I	0.716	0.91
120_I	127_G	0.715	0.91
277_N	310_E	0.712	0.91
88_N	243_E	0.711	0.91
171_F	258_L	0.708	0.90
362_E	365_M	0.700	0.90
359_E	363_E	0.698	0.90
206_E	212_Y	0.696	0.90
123_W	126_M	0.692	0.89
325_L	347_L	0.688	0.89
249_L	347_L	0.688	0.89
271_C	286_L	0.687	0.89
44_H	70_D	0.687	0.89
127_G	131_K	0.685	0.89
241_S	244_R	0.683	0.89
111_R	114_Y	0.683	0.89
58_E	243_E	0.680	0.89
154_P	158_W	0.679	0.89
215_M	226_H	0.679	0.89
269_F	344_E	0.679	0.89
73_D	79_I	0.676	0.88
177_F	215_M	0.672	0.88
257_V	270_T	0.670	0.88
49_G	53_L	0.670	0.88
80_R	88_N	0.669	0.88
78_V	81_A	0.668	0.88
199_R	215_M	0.667	0.88
145_T	150_F	0.667	0.88
261_Q	268_N	0.666	0.88
249_L	297_A	0.666	0.88
145_T	148_D	0.659	0.87
38_I	51_Y	0.658	0.87
256_S	304_P	0.657	0.87
154_P	157_L	0.657	0.87
112_E	115_I	0.656	0.87
20_I	62_F	0.656	0.87
120_I	123_W	0.653	0.87
355_D	359_E	0.653	0.87
14_I	104_R	0.650	0.86
260_C	297_A	0.647	0.86
63_H	66_T	0.646	0.86
116_N	119_L	0.644	0.86
285_I	289_L	0.643	0.86
359_E	362_E	0.642	0.86
148_D	168_L	0.641	0.86
143_N	150_F	0.640	0.85
188_H	197_G	0.634	0.85
78_V	82_K	0.633	0.85
216_P	228_T	0.633	0.85
24_L	81_A	0.628	0.84
44_H	51_Y	0.627	0.84
273_V	277_N	0.627	0.84
249_L	295_F	0.626	0.84
346_L	349_Y	0.624	0.84
247_K	350_K	0.624	0.84
125_W	129_F	0.623	0.84
246_K	290_G	0.621	0.84
41_T	70_D	0.620	0.84
29_N	40_R	0.619	0.83
186_I	258_L	0.617	0.83
257_V	272_A	0.617	0.83
332_T	335_V	0.616	0.83
99_A	102_A	0.612	0.83
52_K	66_T	0.611	0.83
40_R	49_G	0.611	0.83
161_E	174_G	0.608	0.82
252_E	299_D	0.607	0.82
21_L	25_G	0.604	0.82
361_L	364_L	0.604	0.82
276_S	307_H	0.602	0.82
261_Q	270_T	0.600	0.82
297_A	325_L	0.599	0.81
148_D	155_H	0.597	0.81
171_F	233_Y	0.597	0.81
17_I	102_A	0.597	0.81
98_V	102_A	0.597	0.81
18_H	78_V	0.596	0.81
184_I	203_M	0.594	0.81
122_D	127_G	0.594	0.81
44_H	48_G	0.594	0.81
334_Y	342_D	0.592	0.81
66_T	70_D	0.592	0.81
240_N	244_R	0.591	0.81
308_E	312_K	0.590	0.80
197_G	231_N	0.590	0.80
356_K	360_T	0.589	0.80
278_I	298_L	0.587	0.80
361_L	365_M	0.587	0.80
124_E	128_K	0.586	0.80
23_D	77_L	0.585	0.80
100_K	105_T	0.585	0.80
43_C	77_L	0.584	0.80
172_E	195_L	0.584	0.80
283_R	320_Y	0.582	0.80
247_K	344_E	0.580	0.79
197_G	227_Q	0.580	0.79
161_E	255_K	0.579	0.79
197_G	226_H	0.577	0.79
180_H	215_M	0.577	0.79
285_I	288_S	0.577	0.79
30_L	34_Q	0.577	0.79
354_A	371_I	0.576	0.79
226_H	254_E	0.576	0.79
338_Y	345_G	0.576	0.79
282_H	298_L	0.575	0.79
267_S	335_V	0.575	0.79
365_M	369_I	0.574	0.79
143_N	148_D	0.573	0.79
233_Y	257_V	0.573	0.79
115_I	118_D	0.572	0.78
82_K	239_K	0.572	0.78
226_H	282_H	0.572	0.78
188_H	254_E	0.570	0.78
245_F	269_F	0.569	0.78
61_Q	73_D	0.569	0.78
281_F	284_D	0.569	0.78
279_S	282_H	0.566	0.78
191_L	239_K	0.563	0.77
167_T	170_E	0.562	0.77
218_K	223_L	0.562	0.77
360_T	364_L	0.562	0.77
300_K	331_F	0.561	0.77
183_G	228_T	0.560	0.77
157_L	161_E	0.560	0.77
148_D	165_H	0.560	0.77
183_G	216_P	0.560	0.77
113_T	117_N	0.560	0.77
147_L	158_W	0.559	0.77
171_F	174_G	0.556	0.76
267_S	347_L	0.552	0.76
308_E	326_K	0.549	0.76
368_K	371_I	0.548	0.75
77_L	81_A	0.546	0.75
188_H	274_C	0.545	0.75
130_K	225_N	0.544	0.75
280_N	284_D	0.542	0.75
144_E	147_L	0.541	0.75
62_F	76_E	0.540	0.74
238_T	242_I	0.540	0.74
309_T	313_Y	0.539	0.74
114_Y	117_N	0.538	0.74
259_K	332_T	0.537	0.74
180_H	217_L	0.537	0.74
326_K	359_E	0.536	0.74
124_E	129_F	0.534	0.74
173_I	186_I	0.530	0.73
131_K	144_E	0.530	0.73
237_K	261_Q	0.529	0.73
62_F	72_F	0.529	0.73
43_C	72_F	0.528	0.73
334_Y	346_L	0.528	0.73
160_G	205_K	0.526	0.72
200_R	254_E	0.526	0.72
148_D	169_K	0.526	0.72
122_D	126_M	0.525	0.72
78_V	98_V	0.522	0.72
352_S	371_I	0.522	0.72
123_W	131_K	0.521	0.72
154_P	174_G	0.521	0.72
248_V	290_G	0.521	0.72
147_L	169_K	0.520	0.72
240_N	268_N	0.519	0.71
114_Y	118_D	0.519	0.71
180_H	200_R	0.518	0.71
251_F	282_H	0.518	0.71
249_L	269_F	0.518	0.71
40_R	50_S	0.518	0.71
40_R	53_L	0.517	0.71
10_E	13_T	0.517	0.71
294_V	320_Y	0.515	0.71
245_F	268_N	0.514	0.71
183_G	214_Y	0.512	0.70
216_P	254_E	0.511	0.70
24_L	77_L	0.511	0.70
213_K	228_T	0.510	0.70
146_V	150_F	0.510	0.70
125_W	131_K	0.509	0.70
196_I	235_L	0.509	0.70
191_L	289_L	0.509	0.70
255_K	332_T	0.508	0.70
308_E	324_I	0.507	0.70
278_I	314_Q	0.507	0.70
40_R	52_K	0.507	0.70
14_I	107_G	0.506	0.70
23_D	101_I	0.505	0.69
79_I	92_N	0.504	0.69
190_D	231_N	0.503	0.69
257_V	300_K	0.502	0.69
242_I	245_F	0.502	0.69
75_F	89_I	0.502	0.69
249_L	325_L	0.502	0.69
120_I	125_W	0.501	0.69
111_R	115_I	0.500	0.69
45_N	49_G	0.500	0.69
53_L	70_D	0.500	0.69

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2au3A	1	0.8836	100	0.262	Contact Map
1dd9A	1	0.627	100	0.518	Contact Map
4edgA	1	0.6429	100	0.534	Contact Map
1nuiA	1	0.627	100	0.622	Contact Map
1q57A	3	0.5688	100	0.667	Contact Map
1d0qA	2	0.2619	99.9	0.727	Contact Map
1t6t1	2	0.2857	99.5	0.813	Contact Map
2fcjA	2	0.3042	98.3	0.886	Contact Map
3vdpA	2	0.4868	83.5	0.945	Contact Map
1vddA	7	0.4921	82.9	0.945	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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