GREMLIN Database
ISPT - Isoprenyl transferase
UniProt: O31751 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 260 (232)
Sequences: 5046 (3229)
Seq/√Len: 212.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
33_E77_K3.2891.00
175_K194_Y3.1491.00
175_K180_S2.8011.00
233_V242_H2.7291.00
64_V81_L2.6841.00
118_N150_G2.6781.00
143_Q146_A2.5031.00
115_V144_D2.4371.00
43_G53_R2.4271.00
104_E133_H2.4001.00
206_L223_V2.3861.00
108_N137_A2.3771.00
51_L55_A2.3161.00
118_N149_D2.2921.00
70_L244_L2.2021.00
73_E240_E2.1831.00
54_I98_F2.1651.00
242_H245_Q2.1221.00
63_V66_R2.0201.00
203_P226_S2.0201.00
115_V148_N2.0181.00
93_K97_D1.9981.00
167_S196_M1.9891.00
166_V191_F1.9551.00
127_E139_E1.9441.00
126_D167_S1.9431.00
239_K242_H1.9371.00
65_K110_Y1.9361.00
72_N150_G1.9331.00
154_N200_L1.9261.00
135_L139_E1.9161.00
229_V249_E1.8881.00
215_L223_V1.8791.00
71_A243_F1.8561.00
229_V246_A1.7791.00
127_E142_V1.7351.00
78_V201_Q1.7251.00
78_V200_L1.7231.00
123_I138_I1.7131.00
162_R202_D1.7051.00
163_T167_S1.7031.00
31_I76_V1.6711.00
90_K259_G1.6711.00
182_N185_D1.6651.00
129_A158_N1.6431.00
78_V154_N1.6381.00
74_L244_L1.6111.00
48_K234_L1.6061.00
206_L226_S1.5871.00
171_S194_Y1.5641.00
35_I243_F1.5481.00
66_R240_E1.5461.00
122_R152_I1.5421.00
132_A136_R1.5401.00
69_K117_E1.5321.00
169_A191_F1.5311.00
59_E62_K1.5311.00
78_V152_I1.5081.00
55_A58_H1.5051.00
118_N148_N1.4861.00
174_E178_E1.4821.00
164_E196_M1.4791.00
163_T196_M1.4391.00
172_L190_L1.4351.00
37_I67_T1.4221.00
173_A176_V1.4101.00
187_D190_L1.4021.00
140_K144_D1.3981.00
162_R188_E1.3691.00
74_L247_L1.3681.00
172_L186_I1.3671.00
139_E143_Q1.3601.00
127_E135_L1.3521.00
80_T156_A1.3371.00
172_L194_Y1.3301.00
70_L73_E1.2891.00
161_G220_L1.2881.00
247_L251_Q1.2781.00
69_K113_E1.2691.00
37_I79_L1.2591.00
126_D163_T1.2441.00
130_L157_L1.2411.00
192_S202_D1.2211.00
136_R140_K1.2111.00
191_F194_Y1.2091.00
111_L141_A1.1911.00
132_A135_L1.1911.00
120_Q152_I1.1711.00
38_I206_L1.1641.00
177_K183_I1.1571.00
249_E252_Q1.1551.00
104_E137_A1.1481.00
177_K182_N1.1461.00
52_P55_A1.1421.00
126_D129_A1.1401.00
63_V67_T1.1401.00
124_I197_T1.1381.00
189_S193_T1.1371.00
167_S171_S1.1141.00
104_E134_T1.1121.00
80_T219_M1.1111.00
124_I156_A1.1051.00
205_L246_A1.1031.00
165_I191_F1.1011.00
173_A186_I1.0991.00
176_V181_L1.0971.00
81_L153_L1.0961.00
122_R142_V1.0951.00
63_V239_K1.0911.00
84_F89_W1.0861.00
84_F96_V1.0851.00
164_E222_Q1.0791.00
181_L190_L1.0741.00
61_M106_F1.0621.00
103_P157_L1.0531.00
124_I154_N1.0511.00
164_E167_S1.0481.00
223_V226_S1.0461.00
213_I230_F1.0451.00
137_A140_K1.0451.00
35_I247_L1.0421.00
111_L144_D1.0391.00
58_H105_E1.0361.00
119_V151_M1.0281.00
248_G251_Q0.9951.00
123_I127_E0.9931.00
171_S174_E0.9811.00
243_F247_L0.9661.00
35_I76_V0.9631.00
70_L243_F0.9621.00
176_V183_I0.9591.00
190_L194_Y0.9521.00
165_I169_A0.9511.00
174_E177_K0.9511.00
172_L181_L0.9420.99
112_P144_D0.9400.99
81_L155_F0.9280.99
58_H62_K0.9230.99
121_V141_A0.9210.99
68_T153_L0.9180.99
223_V228_F0.9120.99
72_N151_M0.9100.99
35_I71_A0.9100.99
112_P116_E0.9100.99
39_M83_A0.9080.99
134_T137_A0.9080.99
36_A203_P0.9020.99
108_N140_K0.8960.99
86_T160_G0.8910.99
176_V179_G0.8870.99
139_E142_V0.8790.99
122_R154_N0.8720.99
170_K184_E0.8680.99
36_A206_L0.8640.99
67_T70_L0.8640.99
215_L220_L0.8610.99
31_I251_Q0.8570.99
245_Q249_E0.8520.99
79_L151_M0.8460.99
174_E184_E0.8410.99
169_A173_A0.8400.99
68_T119_V0.8380.99
131_P134_T0.8380.99
166_V186_I0.8370.99
96_V100_M0.8210.99
240_E244_L0.8210.99
229_V242_H0.8210.99
76_V247_L0.8150.99
72_N117_E0.8090.98
244_L248_G0.8060.98
173_A177_K0.7980.98
172_L191_F0.7940.98
122_R199_S0.7880.98
166_V169_A0.7860.98
170_K173_A0.7850.98
70_L240_E0.7780.98
128_T135_L0.7760.98
66_R239_K0.7760.98
100_M130_L0.7750.98
210_S256_R0.7750.98
231_T246_A0.7730.98
94_M97_D0.7720.98
57_H95_E0.7700.98
123_I142_V0.7660.98
167_S170_K0.7660.98
74_L243_F0.7620.98
49_R237_D0.7610.98
161_G164_E0.7610.98
241_D244_L0.7600.98
166_V170_K0.7600.98
122_R200_L0.7580.98
55_A59_E0.7550.98
203_P223_V0.7500.97
67_T240_E0.7480.97
175_K193_T0.7470.97
209_T231_T0.7420.97
160_G217_N0.7410.97
158_N259_G0.7400.97
87_E160_G0.7370.97
174_E183_I0.7340.97
190_L193_T0.7330.97
78_V203_P0.7320.97
203_P206_L0.7320.97
89_W158_N0.7250.97
210_S213_I0.7250.97
45_W237_D0.7230.97
105_E109_T0.7190.97
126_D158_N0.7150.97
65_K113_E0.7150.97
213_I249_E0.7120.96
70_L74_L0.7090.96
185_D190_L0.7040.96
220_L224_A0.7040.96
169_A172_L0.7030.96
37_I207_I0.7030.96
191_F195_L0.6940.96
43_G92_P0.6940.96
234_L237_D0.6930.96
103_P106_F0.6910.96
123_I155_F0.6910.96
72_N119_V0.6900.96
192_S195_L0.6890.96
31_I247_L0.6870.96
103_P134_T0.6860.96
66_R69_K0.6790.95
175_K178_E0.6770.95
170_K183_I0.6770.95
173_A181_L0.6770.95
87_E217_N0.6730.95
101_K133_H0.6730.95
68_T151_M0.6730.95
31_I75_G0.6720.95
183_I187_D0.6720.95
77_K152_I0.6680.95
186_I190_L0.6660.95
161_G218_F0.6620.95
181_L186_I0.6600.95
32_P35_I0.6590.95
233_V237_D0.6570.95
193_T199_S0.6540.94
110_Y113_E0.6530.94
103_P155_F0.6520.94
48_K237_D0.6500.94
213_I232_D0.6460.94
161_G215_L0.6450.94
231_T242_H0.6450.94
137_A141_A0.6450.94
134_T138_I0.6440.94
168_A191_F0.6430.94
35_I207_I0.6420.94
69_K73_E0.6370.94
138_I155_F0.6370.94
138_I157_L0.6360.94
62_K109_T0.6320.93
59_E63_V0.6310.93
205_L229_V0.6240.93
212_E256_R0.6230.93
197_T222_Q0.6220.93
30_E251_Q0.6210.93
82_Y217_N0.6200.93
127_E138_I0.6180.93
192_S199_S0.6160.92
71_A79_L0.6150.92
97_D101_K0.6130.92
36_A218_F0.6100.92
180_S194_Y0.6070.92
101_K105_E0.6060.92
173_A183_I0.6030.92
183_I190_L0.6020.92
161_G165_I0.6010.92
31_I74_L0.5970.91
100_M131_P0.5940.91
80_T124_I0.5930.91
238_F246_A0.5920.91
152_I200_L0.5900.91
47_K53_R0.5880.91
57_H102_L0.5870.91
241_D245_Q0.5840.90
107_L111_L0.5830.90
188_E192_S0.5810.90
207_I246_A0.5790.90
215_L228_F0.5780.90
59_E66_R0.5780.90
66_R70_L0.5770.90
136_R139_E0.5750.90
209_T230_F0.5740.90
108_N112_P0.5730.89
103_P107_L0.5720.89
67_T243_F0.5690.89
229_V245_Q0.5680.89
231_T238_F0.5680.89
210_S234_L0.5670.89
113_E116_E0.5670.89
32_P251_Q0.5660.89
181_L185_D0.5650.89
248_G252_Q0.5650.89
170_K174_E0.5630.89
29_G251_Q0.5620.89
104_E108_N0.5610.88
49_R52_P0.5600.88
124_I219_M0.5590.88
183_I186_I0.5590.88
70_L239_K0.5590.88
63_V70_L0.5580.88
49_R53_R0.5540.88
235_W238_F0.5540.88
82_Y164_E0.5530.88
85_S160_G0.5530.88
186_I194_Y0.5510.88
121_V155_F0.5510.88
68_T79_L0.5500.88
225_Y255_R0.5480.87
166_V188_E0.5470.87
227_E255_R0.5440.87
170_K185_D0.5430.87
111_L137_A0.5410.87
115_V147_Q0.5410.87
69_K72_N0.5410.87
253_R256_R0.5400.87
111_L115_V0.5350.86
239_K245_Q0.5340.86
87_E225_Y0.5320.86
114_L153_L0.5310.86
204_E257_F0.5310.86
207_I238_F0.5290.85
192_S221_W0.5280.85
83_A155_F0.5270.85
114_L119_V0.5250.85
244_L251_Q0.5250.85
43_G54_I0.5200.84
193_T198_E0.5200.84
104_E131_P0.5180.84
128_T158_N0.5170.84
68_T72_N0.5160.84
57_H99_L0.5120.84
121_V153_L0.5120.84
173_A180_S0.5120.84
231_T249_E0.5110.83
172_L175_K0.5090.83
82_Y215_L0.5090.83
101_K104_E0.5070.83
89_W100_M0.5070.83
148_N151_M0.5060.83
173_A179_G0.5050.83
227_E253_R0.5030.83
107_L141_A0.5020.82
86_T217_N0.5000.82
176_V186_I0.5000.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1f75A 2 0.8346 100 0.116 Contact Map
2vg3A 2 1 100 0.121 Contact Map
4q9mA 2 0.8692 100 0.125 Contact Map
4h8eA 2 0.9038 100 0.125 Contact Map
3sgvB 2 0.8769 100 0.126 Contact Map
2d2rA 2 0.8346 100 0.143 Contact Map
3ugsB 2 0.8 100 0.157 Contact Map
2vg0A 2 0.8538 100 0.163 Contact Map
3wqlA 2 0.9 97.7 0.913 Contact Map
2infA 2 0.4962 22.5 0.968 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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