GREMLIN Database
K1PF - 1-phosphofructokinase
UniProt: O31714 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 303 (280)
Sequences: 22587 (16968)
Seq/√Len: 1014.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
51_V257_A3.2361.00
54_K77_E2.5881.00
130_V159_V2.5771.00
218_M224_L2.5641.00
225_L269_F2.5141.00
68_K83_V2.4531.00
258_G263_L2.4521.00
264_P267_E2.3151.00
223_A251_V2.2811.00
4_T126_G2.1961.00
149_E174_K2.1541.00
65_E83_V2.1161.00
207_V226_F2.0531.00
182_H218_M2.0521.00
151_C156_A2.0281.00
46_L51_V1.9801.00
258_G271_L1.9631.00
232_Y266_E1.9481.00
48_R74_E1.9211.00
159_V256_L1.9161.00
128_I157_R1.8921.00
5_V43_S1.8781.00
148_A158_V1.8721.00
72_R78_T1.8471.00
203_G226_F1.8421.00
44_R74_E1.8361.00
179_K252_V1.8221.00
250_S275_S1.8091.00
200_V231_V1.8081.00
157_R176_F1.8061.00
58_F115_A1.8061.00
45_L253_A1.7831.00
263_L267_E1.7731.00
224_L233_F1.7661.00
223_A273_V1.7391.00
276_G280_A1.7251.00
70_F73_E1.7141.00
176_F212_E1.7111.00
145_E170_A1.7061.00
225_L232_Y1.6941.00
254_G275_S1.6811.00
247_A280_A1.6651.00
255_F268_A1.6531.00
128_I159_V1.6521.00
177_L213_H1.6301.00
266_E270_R1.6231.00
69_T73_E1.6231.00
215_I255_F1.6161.00
68_K72_R1.6091.00
205_K209_Q1.5951.00
150_A154_Q1.5931.00
213_H227_T1.5891.00
145_E149_E1.5851.00
218_M233_F1.5831.00
237_P274_T1.5761.00
33_K66_Y1.5601.00
146_K149_E1.5541.00
9_P57_G1.5381.00
218_M221_D1.5261.00
234_A269_F1.5211.00
205_K208_E1.5191.00
225_L265_L1.5061.00
146_K150_A1.5001.00
128_I256_L1.4861.00
254_G271_L1.4831.00
46_L257_A1.4731.00
227_T230_A1.4621.00
243_N284_E1.4571.00
61_G65_E1.4541.00
35_P67_I1.4411.00
150_A153_Q1.4251.00
186_G192_A1.4191.00
157_R177_L1.4151.00
152_K176_F1.3911.00
234_A273_V1.3901.00
191_T205_K1.3891.00
237_P270_R1.3711.00
40_I71_L1.3661.00
11_V66_Y1.3601.00
198_E201_P1.3571.00
112_D115_A1.3551.00
87_T92_K1.3521.00
65_E69_T1.3501.00
162_I180_P1.3481.00
113_F146_K1.3361.00
261_K291_V1.3241.00
175_P178_M1.3171.00
200_V204_K1.3151.00
207_V228_N1.3111.00
204_K208_E1.3101.00
70_F74_E1.3081.00
16_H30_Y1.3061.00
35_P70_F1.3061.00
52_A77_E1.3031.00
257_A261_K1.3001.00
152_K174_K1.2971.00
124_Q154_Q1.2961.00
223_A269_F1.2911.00
161_D217_S1.2871.00
239_G281_F1.2681.00
169_K209_Q1.2511.00
87_T90_N1.2421.00
213_H265_L1.2261.00
90_N93_L1.2221.00
179_K217_S1.2091.00
68_K81_S1.2041.00
191_T201_P1.2011.00
204_K231_V1.2011.00
213_H255_F1.2001.00
66_Y69_T1.2001.00
157_R212_E1.2001.00
218_M222_G1.1991.00
255_F272_G1.1961.00
258_G268_A1.1951.00
13_Y31_D1.1911.00
254_G272_G1.1901.00
61_G83_V1.1851.00
204_K226_F1.1841.00
226_F231_V1.1841.00
213_H259_I1.1811.00
278_A282_S1.1731.00
255_F259_I1.1721.00
129_V151_C1.1691.00
51_V260_S1.1651.00
241_L280_A1.1631.00
43_S53_S1.1631.00
65_E68_K1.1581.00
117_L146_K1.1571.00
185_L216_V1.1541.00
149_E153_Q1.1471.00
278_A281_F1.1431.00
237_P273_V1.1381.00
258_G261_K1.1371.00
3_Y46_L1.1351.00
239_G280_A1.1341.00
88_R92_K1.1211.00
217_S251_V1.1201.00
61_G84_K1.1181.00
47_K76_L1.1181.00
267_E270_R1.1171.00
49_H254_G1.1131.00
131_L158_V1.1111.00
13_Y33_K1.1071.00
46_L53_S1.1071.00
61_G85_G1.1031.00
42_V130_V1.1031.00
177_L259_I1.0961.00
237_P277_S1.0941.00
177_L255_F1.0891.00
130_V256_L1.0871.00
78_T81_S1.0781.00
69_T72_R1.0741.00
44_R48_R1.0701.00
213_H225_L1.0681.00
51_V261_K1.0661.00
201_P204_K1.0651.00
241_L244_S1.0631.00
54_K126_G1.0601.00
47_K75_N1.0451.00
212_E228_N1.0391.00
129_V156_A1.0331.00
203_G216_V1.0231.00
138_S142_D1.0231.00
182_H221_D1.0211.00
18_E29_S1.0191.00
16_H32_T1.0111.00
54_K80_F1.0011.00
250_S279_T1.0001.00
66_Y70_F1.0001.00
130_V252_V0.9971.00
46_L253_A0.9961.00
5_V39_G0.9901.00
35_P66_Y0.9891.00
255_F265_L0.9861.00
179_K255_F0.9851.00
160_L175_P0.9821.00
142_D146_K0.9821.00
98_E102_N0.9771.00
68_K82_E0.9741.00
242_V283_E0.9671.00
273_V276_G0.9661.00
45_L250_S0.9621.00
185_L202_Y0.9581.00
9_P67_I0.9571.00
59_V64_G0.9501.00
270_R274_T0.9491.00
59_V68_K0.9481.00
131_L148_A0.9441.00
268_A272_G0.9421.00
263_L271_L0.9311.00
14_I34_Y0.9291.00
89_I92_K0.9281.00
47_K52_A0.9281.00
49_H275_S0.9271.00
236_V273_V0.9221.00
18_E94_K0.9201.00
181_N184_E0.9151.00
241_L281_F0.9151.00
68_K78_T0.9141.00
257_A271_L0.9071.00
13_Y63_T0.9061.00
126_G156_A0.9031.00
189_F202_Y0.8981.00
84_K87_T0.8941.00
259_I268_A0.8941.00
113_F142_D0.8891.00
182_H186_G0.8871.00
234_A270_R0.8811.00
59_V81_S0.8801.00
131_L144_Y0.8771.00
240_K281_F0.8771.00
42_V252_V0.8761.00
161_D179_K0.8691.00
230_A266_E0.8671.00
221_D233_F0.8671.00
28_S101_I0.8651.00
227_T265_L0.8631.00
239_G277_S0.8621.00
264_P268_A0.8501.00
201_P205_K0.8491.00
9_P40_I0.8471.00
222_G236_V0.8431.00
22_V97_D0.8411.00
204_K207_V0.8371.00
9_P55_A0.8371.00
149_E170_A0.8341.00
4_T56_L0.8311.00
234_A266_E0.8281.00
82_E115_A0.8271.00
203_G214_V0.8251.00
172_E175_P0.8231.00
40_I286_G0.8221.00
58_F82_E0.8181.00
251_V273_V0.8161.00
113_F143_T0.8121.00
54_K79_A0.8101.00
180_P185_L0.8071.00
254_G258_G0.8051.00
224_L231_V0.8041.00
79_A119_Q0.7951.00
6_T147_I0.7941.00
47_K53_S0.7941.00
161_D180_P0.7931.00
33_K63_T0.7911.00
89_I93_L0.7911.00
59_V83_V0.7811.00
145_E174_K0.7801.00
6_T129_V0.7761.00
288_E292_Q0.7731.00
159_V179_K0.7601.00
250_S288_E0.7591.00
250_S254_G0.7591.00
250_S276_G0.7581.00
128_I260_S0.7541.00
273_V277_S0.7531.00
43_S55_A0.7511.00
274_T278_A0.7481.00
19_D24_G0.7461.00
99_T102_N0.7461.00
148_A174_K0.7441.00
276_G279_T0.7411.00
232_Y269_F0.7371.00
141_H145_E0.7361.00
32_T283_E0.7351.00
170_A173_M0.7321.00
70_F286_G0.7321.00
123_L126_G0.7301.00
4_T54_K0.7281.00
199_A218_M0.7271.00
200_V226_F0.7181.00
58_F84_K0.7061.00
159_V177_L0.6991.00
271_L291_V0.6991.00
44_R286_G0.6981.00
16_H283_E0.6971.00
99_T103_G0.6931.00
200_V233_F0.6921.00
21_T24_G0.6921.00
227_T232_Y0.6911.00
222_G233_F0.6901.00
220_G238_K0.6891.00
215_I225_L0.6881.00
142_D145_E0.6871.00
200_V224_L0.6861.00
113_F117_L0.6771.00
49_H271_L0.6731.00
123_L156_A0.6721.00
80_F83_V0.6701.00
185_L206_L0.6701.00
11_V63_T0.6671.00
203_G231_V0.6661.00
207_V227_T0.6661.00
203_G207_V0.6641.00
143_T147_I0.6641.00
129_V147_I0.6601.00
79_A122_S0.6591.00
43_S76_L0.6571.00
251_V276_G0.6511.00
12_D36_G0.6471.00
191_T202_Y0.6441.00
88_R93_L0.6431.00
263_L268_A0.6421.00
159_V252_V0.6411.00
88_R91_V0.6401.00
251_V269_F0.6381.00
111_E115_A0.6371.00
87_T110_D0.6371.00
151_C158_V0.6351.00
251_V272_G0.6321.00
38_K163_S0.6321.00
223_A234_A0.6311.00
138_S141_H0.6281.00
49_H257_A0.6271.00
35_P286_G0.6261.00
185_L199_A0.6251.00
52_A127_D0.6251.00
113_F116_F0.6241.00
15_V91_V0.6181.00
3_Y260_S0.6141.00
40_I55_A0.6131.00
246_G288_E0.6121.00
262_Q268_A0.6101.00
145_E148_A0.6061.00
97_D100_E0.6001.00
234_A237_P0.6001.00
117_L150_A0.5991.00
199_A202_Y0.5991.00
86_D92_K0.5981.00
158_V175_P0.5971.00
52_A260_S0.5961.00
114_K117_L0.5931.00
115_A118_E0.5921.00
11_V33_K0.5911.00
85_G88_R0.5901.00
169_K173_M0.5891.00
13_Y89_I0.5891.00
15_V31_D0.5871.00
269_F273_V0.5861.00
128_I177_L0.5831.00
57_G81_S0.5811.00
117_L120_F0.5801.00
257_A291_V0.5791.00
253_A257_A0.5781.00
267_E271_L0.5771.00
3_Y128_I0.5771.00
36_G41_N0.5771.00
169_K172_E0.5761.00
15_V28_S0.5761.00
207_V214_V0.5751.00
100_E103_G0.5731.00
97_D103_G0.5731.00
191_T198_E0.5691.00
215_I251_V0.5681.00
139_L142_D0.5671.00
265_L268_A0.5661.00
122_S126_G0.5651.00
183_H187_E0.5641.00
173_M209_Q0.5641.00
65_E72_R0.5631.00
179_K215_I0.5611.00
127_D156_A0.5601.00
207_V210_G0.5601.00
49_H253_A0.5591.00
85_G90_N0.5581.00
42_V256_L0.5571.00
10_S107_T0.5561.00
101_I104_Q0.5551.00
172_E211_A0.5541.00
207_V229_E0.5511.00
173_M210_G0.5511.00
176_F213_H0.5511.00
185_L226_F0.5501.00
80_F122_S0.5491.00
211_A214_V0.5491.00
178_M214_V0.5491.00
71_L76_L0.5381.00
207_V211_A0.5371.00
116_F120_F0.5371.00
265_L269_F0.5361.00
120_F123_L0.5351.00
128_I259_I0.5341.00
202_Y206_L0.5341.00
98_E103_G0.5341.00
137_S160_L0.5341.00
86_D89_I0.5341.00
215_I269_F0.5331.00
160_L168_L0.5321.00
200_V203_G0.5311.00
219_A244_S0.5291.00
47_K74_E0.5281.00
258_G262_Q0.5271.00
136_P139_L0.5261.00
119_Q122_S0.5261.00
42_V253_A0.5261.00
148_A171_T0.5251.00
199_A203_G0.5241.00
175_P211_A0.5231.00
103_G106_P0.5211.00
171_T175_P0.5211.00
42_V46_L0.5211.00
71_L78_T0.5211.00
11_V67_I0.5201.00
161_D249_D0.5191.00
87_T93_L0.5181.00
114_K118_E0.5181.00
182_H219_A0.5161.00
282_S290_F0.5151.00
11_V35_P0.5151.00
245_V285_L0.5081.00
288_E291_V0.5071.00
104_Q107_T0.5071.00
151_C154_Q0.5071.00
220_G241_L0.5051.00
214_V226_F0.5051.00
115_A119_Q0.5051.00
160_L171_T0.5031.00
120_F124_Q0.5031.00
55_A71_L0.5021.00
23_G27_R0.5011.00
79_A82_E0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ajrA 2 0.9835 100 0.212 Contact Map
2jg5A 1 0.9868 100 0.212 Contact Map
2abqA 2 0.9868 100 0.215 Contact Map
2rbcA 2 0.9571 100 0.217 Contact Map
1rkdA 2 0.9802 100 0.219 Contact Map
3vasA 1 0.9637 100 0.22 Contact Map
2fv7A 2 0.9703 100 0.222 Contact Map
3iq0A 2 0.9604 100 0.23 Contact Map
3hj6A 2 0.8647 100 0.231 Contact Map
3ry7A 2 0.9637 100 0.232 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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