YFJQ - BACSU - GREMLIN Contacts

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YFJQ - Putative metal ion transporter YfjQ

UniProt:	O31543	Sequences: MINITAITTEHQLLKNIPIERVEQPDIAWYWVDFYGPEDTETALLRDFFHFHPLAIEDCFQHMQRPKLDHYDGYRFYVIHALNKETLETEEVDIFQGEKFVVTFHLHETPGIAKVRERLYASPDILKKGPGHISYMIMDQLVDEYFPLVYKIEDRLNEIEESRPHKTYGTLMNEVFDLRTDLLHLRRTIIPMRDLLYRILSLDHVKEQRETKAYFSDIYDHLLKLSEIVESNRDMTSDLRDSYVTLNSNRMNAIMMTLTIVSTIFIPLTFIAGVYGMNFDFMPELHWKYGYFAVLGLMAALVIGMLIWFVHKGWFNIFK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	319 (291)
Sequences:	5385 (3740)
Seq/√Len:	219.3

ECOLI - ZNTB CORA
BACSU - YFJQ CORA

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
280_D	286_H	9.318	1.00
97_G	100_F	4.075	1.00
284_E	291_Y	3.915	1.00
295_L	299_A	3.258	1.00
55_A	95_F	2.965	1.00
289_Y	293_A	2.937	1.00
31_W	103_T	2.862	1.00
292_F	296_G	2.858	1.00
293_A	297_L	2.729	1.00
274_V	295_L	2.714	1.00
303_I	307_I	2.603	1.00
94_I	101_V	2.533	1.00
307_I	311_H	2.445	1.00
33_D	105_H	2.436	1.00
282_M	285_L	2.390	1.00
162_S	174_E	2.389	1.00
79_I	138_M	2.388	1.00
279_F	285_L	2.378	1.00
43_A	47_D	2.366	1.00
290_G	294_V	2.264	1.00
94_I	103_T	2.244	1.00
271_I	294_V	2.229	1.00
289_Y	292_F	2.229	1.00
155_R	174_E	2.201	1.00
265_F	270_F	2.200	1.00
49_F	102_V	2.144	1.00
287_W	293_A	2.141	1.00
288_K	292_F	2.125	1.00
46_R	56_I	2.117	1.00
34_F	45_L	2.068	1.00
270_F	274_V	2.016	1.00
282_M	291_Y	2.002	1.00
101_V	133_I	1.997	1.00
142_V	218_I	1.984	1.00
252_N	255_M	1.928	1.00
55_A	74_Y	1.917	1.00
281_F	288_K	1.883	1.00
287_W	290_G	1.856	1.00
274_V	298_M	1.813	1.00
143_D	221_H	1.799	1.00
306_L	310_V	1.781	1.00
291_Y	295_L	1.770	1.00
279_F	282_M	1.705	1.00
292_F	295_L	1.697	1.00
304_G	308_W	1.687	1.00
77_Y	134_S	1.686	1.00
101_V	134_S	1.643	1.00
38_E	41_E	1.638	1.00
55_A	71_Y	1.632	1.00
186_R	230_E	1.604	1.00
262_S	266_I	1.600	1.00
287_W	291_Y	1.596	1.00
51_F	95_F	1.571	1.00
281_F	292_F	1.522	1.00
67_K	220_D	1.516	1.00
59_C	95_F	1.501	1.00
39_D	43_A	1.474	1.00
283_P	290_G	1.453	1.00
256_M	260_I	1.449	1.00
275_Y	285_L	1.445	1.00
156_L	236_T	1.439	1.00
294_V	297_L	1.435	1.00
275_Y	291_Y	1.430	1.00
139_D	221_H	1.428	1.00
294_V	298_M	1.422	1.00
40_T	43_A	1.411	1.00
64_Q	78_V	1.393	1.00
279_F	290_G	1.388	1.00
275_Y	284_E	1.371	1.00
82_L	185_L	1.371	1.00
279_F	291_Y	1.339	1.00
92_V	141_L	1.338	1.00
277_M	294_V	1.325	1.00
52_H	71_Y	1.323	1.00
271_I	275_Y	1.321	1.00
285_L	291_Y	1.316	1.00
284_E	287_W	1.310	1.00
305_M	309_F	1.307	1.00
135_Y	215_F	1.304	1.00
54_L	57_E	1.303	1.00
101_V	130_P	1.302	1.00
308_W	312_K	1.286	1.00
163_R	239_L	1.269	1.00
275_Y	281_F	1.267	1.00
76_F	95_F	1.258	1.00
96_Q	134_S	1.256	1.00
70_H	75_R	1.256	1.00
266_I	270_F	1.256	1.00
77_Y	94_I	1.252	1.00
139_D	143_D	1.248	1.00
253_A	256_M	1.244	1.00
58_D	67_K	1.233	1.00
197_Y	219_Y	1.207	1.00
219_Y	223_L	1.206	1.00
142_V	221_H	1.206	1.00
179_R	241_D	1.201	1.00
64_Q	91_E	1.197	1.00
248_S	251_M	1.195	1.00
254_I	259_T	1.188	1.00
302_V	306_L	1.181	1.00
267_P	270_F	1.164	1.00
293_A	296_G	1.164	1.00
152_I	178_L	1.153	1.00
37_P	41_E	1.145	1.00
43_A	46_R	1.144	1.00
268_L	298_M	1.142	1.00
306_L	315_F	1.131	1.00
29_W	133_I	1.124	1.00
284_E	292_F	1.124	1.00
295_L	298_M	1.123	1.00
188_T	192_M	1.120	1.00
282_M	292_F	1.119	1.00
259_T	262_S	1.113	1.00
200_L	216_S	1.111	1.00
193_R	223_L	1.109	1.00
115_V	140_Q	1.108	1.00
265_F	268_L	1.105	1.00
29_W	130_P	1.095	1.00
254_I	258_L	1.087	1.00
35_Y	108_E	1.085	1.00
217_D	220_D	1.083	1.00
118_R	136_M	1.081	1.00
280_D	285_L	1.080	1.00
296_G	300_A	1.074	1.00
172_M	243_Y	1.074	1.00
179_R	233_R	1.071	1.00
83_N	88_E	1.069	1.00
259_T	266_I	1.063	1.00
44_L	48_F	1.058	1.00
116_R	120_Y	1.055	1.00
51_F	102_V	1.043	1.00
255_M	262_S	1.041	1.00
274_V	291_Y	1.038	1.00
247_N	251_M	1.037	1.00
193_R	230_E	1.023	1.00
59_C	93_D	1.019	1.00
142_V	222_L	1.018	1.00
42_T	46_R	1.009	1.00
70_H	202_L	1.007	1.00
208_Q	211_T	1.007	1.00
268_L	294_V	1.007	1.00
290_G	293_A	1.007	1.00
57_E	143_D	0.999	1.00
279_F	294_V	0.997	1.00
182_L	232_N	0.995	1.00
183_L	235_M	0.982	1.00
282_M	287_W	0.982	1.00
160_E	180_T	0.981	1.00
115_V	133_I	0.980	1.00
62_H	106_L	0.979	1.00
279_F	284_E	0.975	1.00
274_V	277_M	0.973	1.00
65_R	227_E	0.966	1.00
268_L	271_I	0.964	1.00
179_R	240_R	0.962	1.00
28_A	99_K	0.956	1.00
155_R	181_D	0.954	1.00
272_A	295_L	0.953	1.00
284_E	290_G	0.952	1.00
196_L	222_L	0.951	1.00
117_E	120_Y	0.950	1.00
68_L	77_Y	0.948	1.00
275_Y	283_P	0.944	1.00
113_A	117_E	0.939	1.00
109_T	112_I	0.935	1.00
242_S	256_M	0.934	1.00
33_D	103_T	0.930	0.99
176_F	240_R	0.929	0.99
65_R	191_P	0.923	0.99
146_F	225_L	0.922	0.99
223_L	227_E	0.921	0.99
237_S	241_D	0.914	0.99
94_I	134_S	0.912	0.99
248_S	252_N	0.910	0.99
298_M	302_V	0.906	0.99
64_Q	217_D	0.902	0.99
118_R	122_S	0.901	0.99
124_D	127_K	0.898	0.99
263_T	266_I	0.897	0.99
57_E	139_D	0.891	0.99
44_L	47_D	0.887	0.99
275_Y	282_M	0.880	0.99
162_S	171_L	0.875	0.99
50_H	53_P	0.875	0.99
58_D	217_D	0.873	0.99
80_H	91_E	0.868	0.99
129_G	132_H	0.868	0.99
52_H	55_A	0.867	0.99
275_Y	294_V	0.864	0.99
78_V	93_D	0.864	0.99
262_S	265_F	0.863	0.99
156_L	232_N	0.858	0.99
69_D	216_S	0.854	0.99
246_L	249_N	0.854	0.99
310_V	315_F	0.854	0.99
300_A	304_G	0.854	0.99
25_P	28_A	0.854	0.99
99_K	130_P	0.854	0.99
178_L	236_T	0.850	0.99
131_G	211_T	0.846	0.99
272_A	291_Y	0.844	0.99
277_M	282_M	0.841	0.99
40_T	44_L	0.837	0.99
36_G	106_L	0.836	0.99
190_I	227_E	0.834	0.99
268_L	302_V	0.831	0.99
153_E	232_N	0.828	0.99
304_G	307_I	0.827	0.99
269_T	273_G	0.826	0.99
87_L	181_D	0.814	0.99
164_P	246_L	0.813	0.99
275_Y	278_N	0.812	0.99
269_T	274_V	0.811	0.99
282_M	290_G	0.810	0.99
52_H	58_D	0.808	0.99
52_H	74_Y	0.808	0.99
275_Y	309_F	0.807	0.99
189_I	225_L	0.807	0.99
90_E	105_H	0.806	0.99
27_I	30_Y	0.806	0.99
263_T	268_L	0.800	0.98
114_K	140_Q	0.798	0.98
253_A	266_I	0.798	0.98
271_I	298_M	0.797	0.98
149_V	152_I	0.796	0.98
81_A	109_T	0.796	0.98
287_W	292_F	0.796	0.98
203_D	207_E	0.795	0.98
281_F	290_G	0.794	0.98
112_I	137_I	0.793	0.98
270_F	298_M	0.786	0.98
114_K	117_E	0.780	0.98
68_L	75_R	0.775	0.98
275_Y	287_W	0.775	0.98
182_L	236_T	0.774	0.98
117_E	121_A	0.774	0.98
155_R	158_E	0.773	0.98
248_S	255_M	0.773	0.98
77_Y	96_Q	0.769	0.98
268_L	301_L	0.768	0.98
60_F	169_G	0.759	0.98
118_R	140_Q	0.756	0.98
255_M	273_G	0.755	0.98
283_P	286_H	0.755	0.98
261_V	267_P	0.753	0.98
268_L	272_A	0.750	0.98
115_V	136_M	0.749	0.98
80_H	89_T	0.747	0.98
58_D	221_H	0.744	0.98
89_T	184_H	0.743	0.98
79_I	94_I	0.742	0.98
282_M	294_V	0.737	0.97
260_I	267_P	0.734	0.97
264_I	302_V	0.726	0.97
289_Y	295_L	0.724	0.97
189_I	226_S	0.723	0.97
249_N	256_M	0.722	0.97
68_L	195_L	0.721	0.97
197_Y	223_L	0.721	0.97
250_R	259_T	0.721	0.97
139_D	142_V	0.713	0.97
128_K	210_E	0.711	0.97
275_Y	279_F	0.710	0.97
299_A	303_I	0.709	0.97
265_F	272_A	0.705	0.97
138_M	218_I	0.701	0.97
54_L	214_Y	0.697	0.96
301_L	304_G	0.697	0.96
58_D	220_D	0.694	0.96
251_M	255_M	0.689	0.96
283_P	291_Y	0.689	0.96
297_L	301_L	0.689	0.96
176_F	242_S	0.687	0.96
193_R	197_Y	0.684	0.96
116_R	119_L	0.683	0.96
250_R	254_I	0.682	0.96
135_Y	217_D	0.682	0.96
280_D	287_W	0.679	0.96
228_I	232_N	0.679	0.96
168_Y	171_L	0.672	0.96
54_L	69_D	0.671	0.96
230_E	234_D	0.670	0.96
35_Y	105_H	0.669	0.95
238_D	241_D	0.669	0.95
233_R	241_D	0.667	0.95
209_R	212_K	0.667	0.95
33_D	116_R	0.667	0.95
34_F	104_F	0.663	0.95
37_P	105_H	0.663	0.95
133_I	137_I	0.663	0.95
132_H	214_Y	0.657	0.95
284_E	289_Y	0.653	0.95
288_K	307_I	0.652	0.95
79_I	141_L	0.649	0.95
193_R	219_Y	0.649	0.95
54_L	58_D	0.648	0.95
77_Y	195_L	0.646	0.95
219_Y	222_L	0.644	0.94
34_F	41_E	0.644	0.94
58_D	69_D	0.641	0.94
307_I	313_G	0.639	0.94
272_A	298_M	0.636	0.94
81_A	110_P	0.636	0.94
267_P	301_L	0.632	0.94
125_I	128_K	0.631	0.94
186_R	193_R	0.631	0.94
193_R	226_S	0.630	0.94
277_M	298_M	0.630	0.94
281_F	291_Y	0.628	0.94
32_V	102_V	0.623	0.93
183_L	212_K	0.622	0.93
192_M	224_K	0.621	0.93
257_T	265_F	0.620	0.93
75_R	96_Q	0.619	0.93
92_V	112_I	0.619	0.93
65_R	143_D	0.619	0.93
113_A	174_E	0.618	0.93
59_C	104_F	0.616	0.93
271_I	301_L	0.614	0.93
182_L	229_V	0.614	0.93
190_I	226_S	0.614	0.93
151_K	155_R	0.614	0.93
298_M	309_F	0.612	0.93
37_P	104_F	0.608	0.93
269_T	294_V	0.604	0.92
257_T	283_P	0.604	0.92
260_I	306_L	0.602	0.92
165_H	168_Y	0.602	0.92
200_L	219_Y	0.602	0.92
279_F	287_W	0.601	0.92
82_L	148_L	0.598	0.92
200_L	212_K	0.598	0.92
264_I	268_L	0.597	0.92
284_E	295_L	0.597	0.92
65_R	223_L	0.596	0.92
173_N	246_L	0.596	0.92
218_I	221_H	0.594	0.92
135_Y	146_F	0.593	0.92
215_F	274_V	0.593	0.92
244_V	249_N	0.592	0.92
257_T	261_V	0.592	0.92
81_A	90_E	0.589	0.91
107_H	113_A	0.588	0.91
47_D	50_H	0.587	0.91
103_T	137_I	0.586	0.91
307_I	310_V	0.586	0.91
237_S	242_S	0.583	0.91
144_E	147_P	0.583	0.91
283_P	309_F	0.582	0.91
270_F	273_G	0.581	0.91
297_L	300_A	0.581	0.91
56_I	59_C	0.581	0.91
172_M	242_S	0.580	0.91
40_T	303_I	0.579	0.91
272_A	277_M	0.578	0.91
147_P	225_L	0.576	0.90
310_V	313_G	0.575	0.90
251_M	254_I	0.574	0.90
234_D	238_D	0.573	0.90
167_T	170_T	0.573	0.90
80_H	188_T	0.572	0.90
280_D	284_E	0.570	0.90
53_P	57_E	0.570	0.90
135_Y	214_Y	0.568	0.90
54_L	67_K	0.567	0.90
250_R	253_A	0.565	0.90
135_Y	211_T	0.562	0.89
42_T	104_F	0.561	0.89
63_M	67_K	0.561	0.89
189_I	229_V	0.560	0.89
25_P	189_I	0.560	0.89
290_G	297_L	0.560	0.89
308_W	311_H	0.559	0.89
285_L	295_L	0.559	0.89
81_A	105_H	0.558	0.89
149_V	232_N	0.556	0.89
274_V	282_M	0.554	0.89
85_E	177_D	0.554	0.89
59_C	74_Y	0.553	0.89
257_T	308_W	0.552	0.89
227_E	233_R	0.552	0.89
39_D	299_A	0.552	0.89
237_S	240_R	0.552	0.89
71_Y	216_S	0.552	0.89
52_H	214_Y	0.550	0.88
234_D	239_L	0.549	0.88
105_H	116_R	0.548	0.88
159_I	174_E	0.548	0.88
280_D	288_K	0.546	0.88
128_K	132_H	0.546	0.88
270_F	302_V	0.544	0.88
164_P	173_N	0.543	0.88
152_I	181_D	0.543	0.88
135_Y	218_I	0.541	0.88
157_N	187_R	0.538	0.87
241_D	244_V	0.538	0.87
57_E	67_K	0.536	0.87
291_Y	294_V	0.535	0.87
123_P	128_K	0.534	0.87
139_D	214_Y	0.531	0.87
180_T	235_M	0.530	0.87
32_V	100_F	0.530	0.87
250_R	256_M	0.526	0.86
149_V	198_R	0.525	0.86
148_L	185_L	0.525	0.86
160_E	239_L	0.525	0.86
157_N	193_R	0.524	0.86
110_P	117_E	0.524	0.86
93_D	104_F	0.523	0.86
175_V	187_R	0.523	0.86
273_G	276_G	0.521	0.86
278_N	283_P	0.521	0.86
99_K	120_Y	0.520	0.85
145_Y	225_L	0.520	0.85
301_L	305_M	0.520	0.85
55_A	159_I	0.519	0.85
263_T	267_P	0.519	0.85
64_Q	67_K	0.519	0.85
296_G	303_I	0.518	0.85
284_E	293_A	0.518	0.85
54_L	71_Y	0.516	0.85
61_Q	221_H	0.515	0.85
149_V	190_I	0.515	0.85
259_T	309_F	0.514	0.85
93_D	106_L	0.513	0.85
175_V	226_S	0.511	0.84
288_K	293_A	0.511	0.84
251_M	256_M	0.509	0.84
176_F	181_D	0.507	0.84
176_F	180_T	0.506	0.84
54_L	218_I	0.505	0.84
131_G	206_K	0.503	0.84
131_G	207_E	0.502	0.83
190_I	230_E	0.501	0.83
290_G	298_M	0.500	0.83
42_T	56_I	0.500	0.83

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4i0uA	4	0.9781	100	0.122	Contact Map
4ev6A	3	0.9812	100	0.128	Contact Map
4egwA	1	0.7712	100	0.456	Contact Map
3nvoA	2	0.7492	100	0.505	Contact Map
3ck6A	3	0.7398	100	0.506	Contact Map
2hn1A	2	0.7053	100	0.542	Contact Map
2bbhA	2	0.627	100	0.544	Contact Map
3rkgA	1	0.7085	95.1	0.913	Contact Map
4jo7E	2	0.279	40.1	0.951	Contact Map
3j9tb	1	0.5925	28.2	0.954	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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