DAGK - BACSU - GREMLIN Contacts

Home
GREMLIN
- Submit - Monomer
- Submit - Complex
Contacts
Metagenomics
- Contacts
- Structures
Structures
- List
Old Projects
- Complexes (2014)
- PFAM (2013)

DAGK - Diacylglycerol kinase

UniProt:	O31502	Sequences: MKRARIIYNPTSGREIFKKHLAQVLQKFEQAGYETSTHATTCAGDATHAAKEAALREFDLIIAAGGDGTINEVVNGLAPLDNRPTLGVIPVGTTNDFARALGIPREDILKAADTVINGVARPIDIGQVNGQYFINIAGGGRLTELTYDVPSKLKTMLGQLAYYLKGMEMLPSLRPTEVEIEYDGKLFQGEIMLFLVTLTNSVGGFEKLAPDSSLNDGMFDLMILKKANLAEFIRVATMALRGEHINDQHIIYTKANRVKVNVSEKMQLNLDGEYGGMLPGEFVNLYRHIHVVMPKEKAEQLDD	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	303 (286)
Sequences:	7013 (5214)
Seq/√Len:	308.3

ECOLI - YEGS
BACSU - DAGK YTLR BMRU

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
126_G	133_F	4.436	1.00
259_K	281_E	3.704	1.00
257_R	281_E	3.605	1.00
257_R	283_V	3.448	1.00
127_Q	283_V	3.312	1.00
181_E	259_K	3.070	1.00
179_E	261_N	3.037	1.00
127_Q	285_L	2.924	1.00
122_P	290_H	2.870	1.00
63_A	73_V	2.802	1.00
124_D	217_G	2.557	1.00
42_C	45_D	2.514	1.00
25_L	29_E	2.464	1.00
255_A	258_V	2.430	1.00
225_K	251_I	2.377	1.00
220_D	254_K	2.367	1.00
218_M	256_N	2.344	1.00
218_M	254_K	2.344	1.00
120_A	290_H	2.321	1.00
182_Y	255_A	2.321	1.00
181_E	261_N	2.254	1.00
126_G	219_F	2.240	1.00
179_E	186_L	2.237	1.00
78_A	125_I	2.232	1.00
64_A	91_V	2.224	1.00
179_E	188_Q	2.121	1.00
137_A	282_F	2.075	1.00
60_L	85_T	2.059	1.00
261_N	279_P	2.053	1.00
259_K	279_P	2.031	1.00
121_R	215_N	1.953	1.00
138_G	269_N	1.938	1.00
125_I	288_H	1.926	1.00
231_E	249_H	1.921	1.00
197_T	209_A	1.920	1.00
85_T	120_A	1.884	1.00
45_D	49_A	1.787	1.00
97_F	123_I	1.758	1.00
107_D	110_K	1.745	1.00
183_D	256_N	1.724	1.00
285_L	288_H	1.697	1.00
40_T	46_A	1.694	1.00
70_I	88_V	1.691	1.00
221_L	255_A	1.658	1.00
138_G	142_L	1.658	1.00
27_K	113_D	1.658	1.00
44_G	72_E	1.647	1.00
45_D	48_H	1.627	1.00
124_D	216_D	1.617	1.00
51_K	79_P	1.606	1.00
60_L	292_V	1.573	1.00
127_Q	130_G	1.549	1.00
5_R	58_F	1.532	1.00
191_I	223_I	1.530	1.00
75_N	132_Y	1.508	1.00
177_E	190_E	1.507	1.00
103_I	111_A	1.506	1.00
148_D	169_M	1.486	1.00
221_L	258_V	1.485	1.00
104_P	114_T	1.484	1.00
122_P	287_R	1.465	1.00
181_E	186_L	1.447	1.00
71_N	75_N	1.432	1.00
127_Q	132_Y	1.424	1.00
41_T	45_D	1.419	1.00
120_A	292_V	1.401	1.00
40_T	72_E	1.400	1.00
78_A	132_Y	1.397	1.00
23_Q	109_L	1.387	1.00
196_V	219_F	1.385	1.00
71_N	131_Q	1.369	1.00
223_I	253_T	1.367	1.00
72_E	76_G	1.362	1.00
144_E	169_M	1.356	1.00
178_V	191_I	1.351	1.00
50_A	54_A	1.313	1.00
262_V	266_M	1.312	1.00
196_V	258_V	1.289	1.00
96_D	135_N	1.284	1.00
74_V	134_I	1.270	1.00
101_L	291_V	1.266	1.00
139_G	260_V	1.259	1.00
238_M	247_D	1.255	1.00
71_N	273_E	1.254	1.00
50_A	76_G	1.233	1.00
4_A	60_L	1.230	1.00
181_E	184_G	1.229	1.00
256_N	286_Y	1.219	1.00
52_E	56_R	1.218	1.00
46_A	50_A	1.217	1.00
182_Y	185_K	1.212	1.00
48_H	52_E	1.211	1.00
97_F	135_N	1.209	1.00
126_G	282_F	1.208	1.00
219_F	284_N	1.204	1.00
125_I	132_Y	1.202	1.00
147_Y	267_Q	1.190	1.00
36_S	58_F	1.189	1.00
221_L	253_T	1.188	1.00
100_A	200_N	1.177	1.00
110_K	113_D	1.153	1.00
177_E	188_Q	1.150	1.00
22_A	25_L	1.143	1.00
8_Y	39_A	1.139	1.00
5_R	38_H	1.133	1.00
26_Q	30_Q	1.133	1.00
63_A	86_L	1.131	1.00
131_Q	275_G	1.128	1.00
132_Y	285_L	1.124	1.00
61_I	77_L	1.119	1.00
104_P	110_K	1.119	1.00
83_R	122_P	1.119	1.00
63_A	69_T	1.109	1.00
48_H	51_K	1.107	1.00
113_D	117_N	1.107	1.00
228_N	231_E	1.106	1.00
97_F	101_L	1.103	1.00
231_E	234_R	1.103	1.00
6_I	62_I	1.100	1.00
4_A	62_I	1.099	1.00
64_A	89_I	1.097	1.00
223_I	251_I	1.094	1.00
27_K	112_A	1.090	1.00
146_T	269_N	1.082	1.00
108_I	112_A	1.075	1.00
216_D	220_D	1.073	1.00
96_D	204_G	1.069	1.00
182_Y	253_T	1.062	1.00
47_T	76_G	1.062	1.00
128_V	282_F	1.060	1.00
62_I	87_G	1.052	1.00
142_L	269_N	1.051	1.00
54_A	76_G	1.050	1.00
165_K	168_E	1.047	1.00
133_F	137_A	1.046	1.00
125_I	134_I	1.036	1.00
74_V	289_I	1.033	1.00
141_R	145_L	1.032	1.00
47_T	51_K	1.030	1.00
135_N	199_T	1.030	1.00
185_K	253_T	1.030	1.00
96_D	203_G	1.020	1.00
268_L	280_G	1.016	1.00
190_E	226_K	1.012	1.00
54_A	80_L	1.009	1.00
196_V	282_F	1.008	1.00
89_I	111_A	1.008	1.00
46_A	72_E	1.005	1.00
98_A	103_I	1.003	1.00
27_K	30_Q	1.002	1.00
38_H	52_E	1.002	1.00
136_I	195_L	1.001	1.00
50_A	53_A	1.001	1.00
142_L	202_V	0.999	1.00
128_V	270_L	0.994	1.00
99_R	201_S	0.974	1.00
265_K	277_M	0.968	1.00
74_V	86_L	0.962	1.00
222_M	252_Y	0.958	1.00
220_D	252_Y	0.957	1.00
89_I	108_I	0.955	1.00
184_G	257_R	0.954	1.00
115_V	292_V	0.947	1.00
8_Y	37_T	0.946	1.00
165_K	169_M	0.944	1.00
96_D	205_F	0.943	1.00
189_G	225_K	0.942	1.00
267_Q	274_Y	0.940	1.00
270_L	275_G	0.937	1.00
187_F	251_I	0.935	1.00
194_F	223_I	0.934	1.00
107_D	111_A	0.932	1.00
80_L	84_P	0.927	1.00
31_A	113_D	0.925	1.00
40_T	45_D	0.921	1.00
62_I	115_V	0.920	1.00
164_L	168_E	0.918	1.00
150_P	153_L	0.915	1.00
27_K	109_L	0.904	1.00
80_L	83_R	0.891	1.00
54_A	57_E	0.890	1.00
128_V	137_A	0.889	1.00
147_Y	274_Y	0.886	1.00
70_I	97_F	0.879	1.00
183_D	255_A	0.879	1.00
23_Q	26_Q	0.878	1.00
7_I	50_A	0.875	1.00
118_G	292_V	0.863	1.00
123_I	291_V	0.859	1.00
128_V	280_G	0.858	1.00
42_C	72_E	0.858	1.00
157_L	164_L	0.852	1.00
88_V	97_F	0.842	1.00
215_N	287_R	0.841	1.00
83_R	290_H	0.839	1.00
262_V	278_L	0.838	1.00
206_E	240_L	0.838	1.00
94_T	142_L	0.836	1.00
51_K	55_L	0.836	1.00
123_I	214_L	0.835	1.00
63_A	70_I	0.835	1.00
29_E	35_T	0.834	1.00
111_A	116_I	0.828	1.00
209_A	212_S	0.826	1.00
136_I	197_T	0.824	1.00
21_L	25_L	0.824	1.00
151_S	154_K	0.824	1.00
97_F	134_I	0.822	1.00
90_P	111_A	0.820	1.00
238_M	243_E	0.817	1.00
206_E	239_A	0.817	1.00
198_L	220_D	0.816	1.00
212_S	220_D	0.814	1.00
22_A	26_Q	0.811	1.00
158_G	162_Y	0.809	1.00
85_T	290_H	0.807	1.00
8_Y	64_A	0.803	1.00
112_A	116_I	0.802	1.00
151_S	155_T	0.801	1.00
104_P	111_A	0.801	1.00
87_G	292_V	0.800	1.00
58_F	84_P	0.798	1.00
94_T	162_Y	0.797	1.00
150_P	154_K	0.796	1.00
51_K	76_G	0.795	1.00
202_V	208_L	0.792	1.00
149_V	153_L	0.791	1.00
153_L	156_M	0.790	1.00
65_G	69_T	0.789	1.00
191_I	194_F	0.787	1.00
129_N	281_E	0.785	1.00
50_A	61_I	0.782	1.00
182_Y	258_V	0.782	1.00
135_N	269_N	0.780	0.99
200_N	207_K	0.779	0.99
128_V	131_Q	0.779	0.99
129_N	283_V	0.776	0.99
152_K	156_M	0.775	0.99
133_F	270_L	0.768	0.99
114_T	118_G	0.762	0.99
199_T	214_L	0.761	0.99
137_A	270_L	0.759	0.99
60_L	115_V	0.751	0.99
54_A	84_P	0.751	0.99
196_V	221_L	0.750	0.99
108_I	111_A	0.749	0.99
77_L	80_L	0.748	0.99
258_V	282_F	0.748	0.99
189_G	251_I	0.740	0.99
44_G	48_H	0.739	0.99
195_L	202_V	0.738	0.99
98_A	105_R	0.738	0.99
138_G	195_L	0.735	0.99
173_L	192_M	0.735	0.99
100_A	214_L	0.734	0.99
7_I	46_A	0.731	0.99
38_H	49_A	0.731	0.99
183_D	257_R	0.730	0.99
96_D	136_I	0.729	0.99
124_D	198_L	0.728	0.99
103_I	114_T	0.728	0.99
125_I	285_L	0.725	0.99
177_E	263_S	0.723	0.99
42_C	48_H	0.722	0.99
148_D	165_K	0.721	0.99
161_A	165_K	0.721	0.99
197_T	202_V	0.720	0.99
70_I	291_V	0.720	0.99
178_V	262_V	0.716	0.99
268_L	277_M	0.715	0.99
229_L	232_F	0.709	0.99
60_L	87_G	0.703	0.99
46_A	76_G	0.703	0.99
87_G	115_V	0.700	0.99
50_A	73_V	0.700	0.99
8_Y	17_F	0.699	0.99
149_V	152_K	0.699	0.99
141_R	192_M	0.697	0.99
99_R	105_R	0.695	0.99
44_G	47_T	0.695	0.99
47_T	75_N	0.692	0.99
51_K	80_L	0.692	0.99
47_T	79_P	0.692	0.99
124_D	215_N	0.688	0.99
267_Q	277_M	0.688	0.99
63_A	88_V	0.687	0.99
29_E	32_G	0.682	0.99
77_L	86_L	0.681	0.99
7_I	73_V	0.679	0.99
96_D	100_A	0.679	0.99
18_K	39_A	0.678	0.99
27_K	31_A	0.678	0.99
74_V	132_Y	0.677	0.99
97_F	198_L	0.671	0.98
78_A	288_H	0.669	0.98
136_I	269_N	0.669	0.98
31_A	116_I	0.668	0.98
8_Y	21_L	0.668	0.98
110_K	114_T	0.666	0.98
181_E	257_R	0.666	0.98
96_D	99_R	0.666	0.98
59_D	85_T	0.663	0.98
151_S	156_M	0.662	0.98
115_V	118_G	0.660	0.98
234_R	247_D	0.659	0.98
197_T	220_D	0.659	0.98
93_T	135_N	0.651	0.98
167_M	171_P	0.646	0.98
109_L	112_A	0.646	0.98
71_N	132_Y	0.643	0.98
158_G	161_A	0.643	0.98
96_D	142_L	0.640	0.98
139_G	194_F	0.639	0.98
139_G	268_L	0.638	0.98
55_L	80_L	0.638	0.98
236_A	244_H	0.637	0.98
154_K	161_A	0.634	0.98
208_L	222_M	0.634	0.98
193_L	197_T	0.634	0.98
18_K	22_A	0.633	0.98
143_T	269_N	0.632	0.98
21_L	39_A	0.631	0.98
61_I	73_V	0.629	0.98
243_E	246_N	0.628	0.98
75_N	131_Q	0.628	0.98
40_T	76_G	0.627	0.98
158_G	170_L	0.626	0.98
96_D	269_N	0.623	0.98
184_G	187_F	0.620	0.97
136_I	199_T	0.619	0.97
49_A	53_A	0.617	0.97
53_A	58_F	0.616	0.97
265_K	278_L	0.611	0.97
125_I	289_I	0.609	0.97
87_G	114_T	0.609	0.97
88_V	98_A	0.609	0.97
20_H	23_Q	0.609	0.97
101_L	214_L	0.606	0.97
28_F	31_A	0.605	0.97
175_P	226_K	0.603	0.97
230_A	233_I	0.602	0.97
196_V	260_V	0.602	0.97
237_T	241_R	0.601	0.97
123_I	289_I	0.599	0.97
180_I	260_V	0.597	0.97
46_A	73_V	0.597	0.97
78_A	285_L	0.594	0.97
109_L	113_D	0.593	0.97
94_T	205_F	0.591	0.97
96_D	146_T	0.591	0.97
5_R	36_S	0.590	0.97
169_M	173_L	0.586	0.96
71_N	74_V	0.586	0.96
100_A	199_T	0.586	0.96
239_A	244_H	0.585	0.96
49_A	52_E	0.584	0.96
268_L	278_L	0.583	0.96
234_R	238_M	0.583	0.96
225_K	249_H	0.582	0.96
233_I	241_R	0.581	0.96
154_K	157_L	0.579	0.96
46_A	49_A	0.576	0.96
179_E	263_S	0.575	0.96
44_G	76_G	0.574	0.96
87_G	111_A	0.571	0.96
17_F	20_H	0.569	0.96
69_T	73_V	0.567	0.96
139_G	266_M	0.567	0.96
135_N	146_T	0.567	0.96
176_T	266_M	0.567	0.96
144_E	267_Q	0.565	0.96
216_D	254_K	0.565	0.96
238_M	246_N	0.564	0.96
157_L	162_Y	0.564	0.96
178_V	194_F	0.563	0.96
168_E	171_P	0.561	0.95
124_D	220_D	0.561	0.95
53_A	57_E	0.560	0.95
77_L	84_P	0.558	0.95
137_A	268_L	0.557	0.95
153_L	157_L	0.553	0.95
121_R	213_S	0.551	0.95
7_I	49_A	0.549	0.95
6_I	35_T	0.547	0.95
234_R	248_Q	0.547	0.95
52_E	55_L	0.547	0.95
222_M	244_H	0.546	0.95
41_T	48_H	0.545	0.95
180_I	194_F	0.544	0.95
135_N	142_L	0.543	0.95
235_V	247_D	0.542	0.95
27_K	116_I	0.542	0.95
107_D	114_T	0.542	0.95
149_V	161_A	0.540	0.94
53_A	63_A	0.538	0.94
69_T	138_G	0.537	0.94
185_K	281_E	0.537	0.94
6_I	64_A	0.535	0.94
13_G	93_T	0.533	0.94
40_T	44_G	0.533	0.94
144_E	147_Y	0.530	0.94
153_L	161_A	0.530	0.94
114_T	117_N	0.528	0.94
177_E	189_G	0.527	0.94
149_V	154_K	0.525	0.94
97_F	199_T	0.524	0.94
38_H	45_D	0.524	0.94
21_L	35_T	0.521	0.93
94_T	204_G	0.520	0.93
142_L	145_L	0.519	0.93
28_F	116_I	0.519	0.93
227_A	231_E	0.517	0.93
160_L	163_Y	0.517	0.93
244_H	250_I	0.516	0.93
86_L	289_I	0.515	0.93
182_Y	186_L	0.514	0.93
16_I	20_H	0.512	0.93
187_F	253_T	0.512	0.93
148_D	168_E	0.510	0.93
135_N	203_G	0.508	0.92
229_L	233_I	0.507	0.92
45_D	72_E	0.507	0.92
183_D	186_L	0.505	0.92
211_D	245_I	0.504	0.92
139_G	262_V	0.503	0.92
101_L	199_T	0.502	0.92
150_P	156_M	0.501	0.92
90_P	103_I	0.500	0.92
233_I	236_A	0.500	0.92

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4werA	2	0.9307	100	0.181	Contact Map
4v24A	1	0.9604	100	0.187	Contact Map
2qv7A	2	0.9538	100	0.193	Contact Map
3s40A	3	0.9703	100	0.212	Contact Map
2bonA	1	0.9373	100	0.22	Contact Map
1u0tA	4	0.802	100	0.581	Contact Map
2an1A	4	0.8152	100	0.587	Contact Map
1z0sA	4	0.7525	100	0.593	Contact Map
1yt5A	4	0.7822	100	0.599	Contact Map
2i2cA	3	0.8086	100	0.608	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0097 seconds.