GREMLIN Database
CHIS - Chitosanase
UniProt: O07921 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 277 (233)
Sequences: 266 (156)
Seq/√Len: 10.2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
199_N205_S6.0431.00
93_E112_R4.1050.99
62_Y146_D3.7740.98
127_G131_A3.3590.96
184_D193_A3.2780.96
98_A147_K3.0800.94
103_K131_A2.8670.91
83_T88_A2.7840.90
107_Y127_G2.7320.89
96_T105_K2.7270.89
197_R226_D2.4490.83
94_V98_A2.3910.81
55_N64_Y2.3200.79
221_D250_R2.2430.77
142_R146_D2.2040.76
124_N129_A2.1120.72
224_Y247_D2.1110.72
124_N131_A2.0810.71
184_D192_Y2.0350.69
243_V247_D2.0040.68
225_D229_N2.0000.68
86_G115_A1.9800.67
44_R48_Q1.9450.66
94_V147_K1.9310.65
103_K134_S1.8930.63
47_E72_R1.8680.62
135_L138_D1.8670.62
139_K142_R1.8660.62
83_T194_L1.8660.62
113_R204_G1.8330.61
124_N127_G1.8300.61
194_L222_V1.8230.60
106_K131_A1.8190.60
167_K259_N1.7650.58
57_T60_I1.7450.57
171_A264_I1.7440.57
27_L30_S1.7150.56
107_Y134_S1.7000.55
107_Y131_A1.6680.53
99_V138_D1.6610.53
49_L166_L1.6190.51
130_S134_S1.6080.51
179_V191_F1.5920.50
197_R261_N1.5880.50
120_D123_S1.5780.49
72_R230_P1.5440.48
160_R169_A1.5060.46
193_A197_R1.4960.45
148_V152_L1.4800.45
143_A146_D1.4630.44
64_Y68_L1.4620.44
83_T135_L1.4620.44
206_P223_R1.4590.44
181_Q240_R1.4580.44
166_L215_W1.4400.43
72_R223_R1.4370.43
194_L253_A1.4180.42
72_R240_R1.4140.42
142_R179_V1.3850.40
181_Q230_P1.3820.40
187_D190_S1.3800.40
204_G208_D1.3720.40
107_Y124_N1.3710.40
82_T148_V1.3640.39
49_L56_G1.3520.39
189_D197_R1.3510.39
101_N179_V1.3320.38
190_S196_K1.3190.37
106_K159_K1.3060.37
159_K213_K1.3060.37
140_E144_A1.3050.37
139_K265_H1.3010.37
57_T91_V1.2880.36
143_A147_K1.2750.36
251_S255_E1.2740.36
197_R225_D1.2610.35
64_Y72_R1.2550.35
94_V139_K1.2430.34
65_V76_C1.2410.34
190_S226_D1.2370.34
63_G134_S1.2370.34
140_E167_K1.2360.34
39_N137_N1.2350.34
88_A141_F1.2310.34
27_L36_A1.1850.32
49_L252_I1.1730.31
86_G138_D1.1650.31
51_S86_G1.1600.31
31_E34_F1.1580.31
47_E61_Q1.1430.30
113_R196_K1.1320.30
189_D225_D1.1050.29
113_R267_R1.0960.28
142_R160_R1.0830.28
34_F63_G1.0830.28
52_I266_V1.0730.28
230_P240_R1.0670.27
40_K118_E1.0640.27
101_N105_K1.0550.27
27_L31_E1.0510.27
130_S159_K1.0510.27
43_K150_D1.0510.27
64_Y223_R1.0450.27
185_G192_Y1.0370.26
131_A146_D1.0310.26
126_K129_A1.0300.26
27_L32_T1.0270.26
39_N101_N1.0210.26
103_K107_Y1.0190.26
64_Y190_S1.0160.25
28_M31_E1.0130.25
31_E36_A1.0010.25
80_G87_D1.0000.25
148_V151_H0.9960.25
189_D229_N0.9960.25
29_M33_V0.9870.24
239_W242_S0.9830.24
73_G81_F0.9770.24
197_R201_K0.9760.24
179_V185_G0.9750.24
91_V155_Q0.9740.24
101_N121_D0.9720.24
76_C82_T0.9670.24
196_K267_R0.9640.24
196_K200_K0.9540.23
30_S39_N0.9530.23
42_Q181_Q0.9520.23
40_K63_G0.9500.23
92_V111_L0.9500.23
50_T122_T0.9480.23
72_R239_W0.9470.23
121_D208_D0.9460.23
253_A258_Y0.9450.23
86_G243_V0.9440.23
180_I195_I0.9430.23
193_A196_K0.9370.23
61_Q68_L0.9300.23
97_K139_K0.9290.23
97_K113_R0.9270.22
103_K106_K0.9270.22
82_T105_K0.9210.22
181_Q246_V0.9170.22
187_D229_N0.9110.22
75_T182_H0.9010.22
72_R181_Q0.8980.22
133_K197_R0.8920.21
61_Q135_L0.8900.21
47_E230_P0.8810.21
157_A255_E0.8720.21
155_Q161_S0.8710.21
238_E241_E0.8630.20
227_L239_W0.8620.20
97_K265_H0.8610.20
197_R200_K0.8610.20
86_G160_R0.8600.20
91_V152_L0.8560.20
199_N207_K0.8560.20
95_Y182_H0.8470.20
132_W219_F0.8470.20
101_N169_A0.8440.20
187_D217_N0.8420.20
230_P239_W0.8330.20
189_D226_D0.8320.20
73_G219_F0.8290.19
61_Q138_D0.8280.19
156_P176_Y0.8250.19
43_K268_S0.8210.19
28_M32_T0.8190.19
40_K121_D0.8190.19
201_K254_K0.8180.19
195_I210_I0.8150.19
174_V185_G0.8150.19
39_N94_V0.8120.19
114_L122_T0.8110.19
153_Y176_Y0.8110.19
47_E57_T0.8090.19
204_G207_K0.8010.19
47_E181_Q0.8010.19
163_N255_E0.7950.19
36_A40_K0.7940.18
83_T138_D0.7900.18
83_T222_V0.7870.18
77_G145_Q0.7800.18
36_A39_N0.7790.18
93_E113_R0.7730.18
185_G190_S0.7720.18
143_A259_N0.7710.18
139_K143_A0.7700.18
117_E133_K0.7690.18
160_R191_F0.7680.18
196_K228_M0.7680.18
178_T219_F0.7670.18
67_R175_M0.7640.18
103_K135_L0.7620.18
73_G87_D0.7550.17
108_L246_V0.7500.17
115_A129_A0.7490.17
99_V140_E0.7490.17
55_N268_S0.7470.17
140_E143_A0.7470.17
200_K224_Y0.7470.17
30_S34_F0.7430.17
75_T223_R0.7280.17
217_N250_R0.7260.17
95_Y223_R0.7240.17
132_W171_A0.7210.17
164_A172_R0.7190.16
184_D197_R0.7190.16
37_G140_E0.7180.16
83_T239_W0.7110.16
62_Y157_A0.7110.16
27_L33_V0.7040.16
51_S61_Q0.7040.16
179_V184_D0.7040.16
36_A163_N0.7030.16
88_A181_Q0.7030.16
196_K214_K0.6950.16
130_S254_K0.6950.16
35_A39_N0.6940.16
245_R260_L0.6940.16
86_G90_E0.6940.16
224_Y241_E0.6930.16
43_K47_E0.6910.16
144_A191_F0.6870.16
158_M180_I0.6850.16
34_F190_S0.6820.16
81_F132_W0.6810.16
52_I92_V0.6780.15
196_K254_K0.6770.15
106_K204_G0.6760.15
181_Q239_W0.6720.15
63_G130_S0.6700.15
34_F91_V0.6690.15
190_S193_A0.6650.15
64_Y206_P0.6580.15
102_N178_T0.6540.15
164_A175_M0.6520.15
113_R155_Q0.6500.15
31_E35_A0.6490.15
174_V179_V0.6490.15
107_Y146_D0.6480.15
218_K222_V0.6460.15
131_A242_S0.6460.15
47_E239_W0.6450.15
99_V103_K0.6410.15
173_A227_L0.6390.15
35_A101_N0.6370.14
130_S155_Q0.6370.14
82_T112_R0.6370.14
99_V106_K0.6310.14
201_K204_G0.6300.14
41_D256_N0.6290.14
42_Q246_V0.6260.14
31_E137_N0.6260.14
81_F87_D0.6210.14
127_G134_S0.6210.14
73_G80_G0.6190.14
118_E241_E0.6170.14
187_D255_E0.6170.14
107_Y126_K0.6150.14
142_R247_D0.6130.14
133_K200_K0.6100.14
46_A173_A0.6090.14
45_R181_Q0.6060.14
106_K130_S0.6030.14
65_V129_A0.5990.14
161_S166_L0.5970.14
84_A182_H0.5970.14
181_Q227_L0.5960.14
35_A205_S0.5950.14
87_D219_F0.5950.14
208_D254_K0.5950.14
135_L211_D0.5920.13
90_E254_K0.5920.13
192_Y196_K0.5900.13
175_M195_I0.5870.13
101_N137_N0.5840.13
175_M252_I0.5840.13
39_N42_Q0.5820.13
157_A222_V0.5820.13
70_D245_R0.5820.13
99_V142_R0.5800.13
90_E204_G0.5800.13
81_F141_F0.5800.13
80_G219_F0.5750.13
39_N82_T0.5750.13
134_S143_A0.5750.13
39_N144_A0.5750.13
71_G110_E0.5740.13
189_D265_H0.5730.13
70_D260_L0.5720.13
88_A135_L0.5710.13
146_D176_Y0.5700.13
145_Q156_P0.5690.13
200_K228_M0.5690.13
76_C132_W0.5660.13
174_V186_D0.5660.13
42_Q169_A0.5630.13
94_V201_K0.5630.13
105_K256_N0.5630.13
32_T91_V0.5620.13
62_Y98_A0.5610.13
81_F216_L0.5560.13
37_G214_K0.5460.13
247_D253_A0.5460.13
143_A188_P0.5430.12
60_I72_R0.5380.12
67_R198_T0.5380.12
62_Y193_A0.5370.12
127_G142_R0.5360.12
201_K217_N0.5350.12
133_K152_L0.5340.12
47_E60_I0.5320.12
121_D196_K0.5320.12
61_Q90_E0.5310.12
211_D214_K0.5280.12
62_Y226_D0.5280.12
141_F145_Q0.5260.12
98_A254_K0.5260.12
42_Q142_R0.5260.12
199_N208_D0.5250.12
196_K213_K0.5250.12
31_E228_M0.5230.12
94_V265_H0.5220.12
34_F62_Y0.5210.12
108_L136_A0.5210.12
103_K161_S0.5190.12
35_A196_K0.5170.12
50_T252_I0.5170.12
131_A244_A0.5150.12
61_Q83_T0.5150.12
66_E246_V0.5150.12
132_W216_L0.5120.12
186_D192_Y0.5120.12
134_S251_S0.5120.12
64_Y186_D0.5100.12
38_L247_D0.5100.12
27_L137_N0.5090.12
54_E223_R0.5090.12
39_N160_R0.5080.12
130_S214_K0.5060.12
74_Y175_M0.5050.12
100_P131_A0.5040.12
71_G105_K0.5040.12
66_E85_T0.5030.12
60_I267_R0.5030.12
121_D254_K0.5020.12
177_D245_R0.5010.12
32_T211_D0.5010.12
202_A218_K0.5000.12
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ilyA 1 0.8881 100 0.21 Contact Map
4oltA 1 0.8412 100 0.215 Contact Map
1qgiA 1 0.8917 100 0.29 Contact Map
4nsoA 1 0.6679 99.7 0.83 Contact Map
4nooA 1 0.6534 97.7 0.919 Contact Map
2nr7A 1 0.5126 73.6 0.958 Contact Map
4grhA 1 0.4332 26.5 0.969 Contact Map
1i1qA 2 0.4332 25.2 0.969 Contact Map
3h9mA 1 0.3538 23.4 0.97 Contact Map
1k0eA 1 0.4404 13.4 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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