GLSA2 - BACSU - GREMLIN Contacts

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GLSA2 - Glutaminase 2

UniProt:	O07637	Sequences: MVCQHNDELEALVKKAKKVTDKGEVASYIPALAKADKHDLSVAIYYSNNVCLSAGDVEKTFTLQSISKVLSLALVLMEYGKDKVFSYVGQEPTGDPFNSIIKLETVNPSKPLNPMINAGALVVTSLIRGRTVKERLDYLLSFIRRLTNNQEITYCREVAESEYSTSMINRAMCYYMKQYGIFEDDVEAVMDLYTKQCAIEMNSLDLAKIGSVFALNGRHPETGEQVISKDVARICKTFMVTCGMYNASGEFAIKVGIPAKSGVSGGIMGISPYDFGIGIFGPALDEKGNSIAGVKLLEIMSEMYRLSIF	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	309 (299)
Sequences:	1602 (1075)
Seq/√Len:	62.2

ECOLI - GLSA1 GLSA2
BACSU - GLSA2 GLSA1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
155_C	200_E	6.183	1.00
145_R	224_E	4.863	1.00
167_M	174_Y	4.293	1.00
39_D	59_K	4.171	1.00
218_R	225_Q	3.407	1.00
166_S	194_T	3.395	1.00
70_L	209_I	3.351	1.00
45_Y	207_A	3.333	1.00
78_E	138_Y	3.297	1.00
41_S	203_S	3.025	1.00
6_N	10_E	2.976	1.00
210_G	268_M	2.909	1.00
15_K	299_I	2.804	1.00
11_A	303_M	2.604	1.00
40_L	296_L	2.574	1.00
257_I	269_G	2.535	1.00
24_E	27_S	2.533	1.00
53_S	57_V	2.469	1.00
88_V	123_V	2.454	1.00
247_A	285_D	2.393	1.00
254_K	298_E	2.371	1.00
60_T	205_D	2.261	1.00
66_I	266_G	2.251	1.00
19_V	295_K	2.218	1.00
14_K	17_K	2.210	1.00
35_A	39_D	2.124	1.00
46_Y	50_V	2.049	1.00
259_A	269_G	2.028	1.00
163_Y	191_D	2.027	1.00
137_D	141_S	2.013	1.00
19_V	22_K	1.998	1.00
26_A	29_I	1.944	1.00
240_V	252_A	1.916	1.00
31_A	34_K	1.910	1.00
279_I	293_G	1.902	1.00
58_E	204_L	1.894	1.00
63_L	67_S	1.890	1.00
83_K	86_S	1.890	1.00
10_E	54_A	1.881	1.00
140_L	199_I	1.875	1.00
43_A	204_L	1.863	1.00
45_Y	51_C	1.859	1.00
219_H	222_T	1.825	1.00
28_Y	263_V	1.807	1.00
172_M	176_M	1.777	1.00
13_V	40_L	1.763	0.99
31_A	157_E	1.760	0.99
67_S	196_Q	1.755	0.99
163_Y	194_T	1.747	0.99
31_A	161_S	1.733	0.99
42_V	296_L	1.725	0.99
77_M	228_S	1.720	0.99
71_S	139_L	1.706	0.99
93_T	116_I	1.669	0.99
163_Y	166_S	1.659	0.99
281_G	292_A	1.658	0.99
60_T	202_N	1.640	0.99
51_C	204_L	1.638	0.99
269_G	297_L	1.621	0.99
13_V	54_A	1.605	0.99
121_L	194_T	1.605	0.99
156_R	195_K	1.599	0.99
42_V	54_A	1.595	0.99
156_R	160_E	1.583	0.99
84_V	126_L	1.559	0.99
167_M	177_K	1.543	0.99
16_A	299_I	1.525	0.98
43_A	53_S	1.519	0.98
66_I	69_V	1.507	0.98
77_M	231_V	1.502	0.98
29_I	32_L	1.498	0.98
124_T	192_L	1.459	0.98
73_A	212_V	1.455	0.98
39_D	56_D	1.441	0.98
53_S	58_E	1.441	0.98
219_H	226_V	1.428	0.98
271_S	275_F	1.413	0.97
295_K	298_E	1.407	0.97
139_L	196_Q	1.380	0.97
244_M	259_A	1.374	0.97
299_I	303_M	1.373	0.97
58_E	202_N	1.368	0.97
35_A	264_S	1.362	0.97
208_K	219_H	1.361	0.97
172_M	175_Y	1.355	0.97
36_D	39_D	1.343	0.97
143_I	152_I	1.341	0.97
43_A	203_S	1.339	0.97
34_K	157_E	1.334	0.96
26_A	263_V	1.299	0.96
142_F	146_L	1.287	0.96
72_L	85_F	1.281	0.96
129_G	184_D	1.274	0.95
62_T	158_V	1.268	0.95
210_G	276_G	1.267	0.95
121_L	166_S	1.248	0.95
101_I	105_T	1.248	0.95
122_V	176_M	1.245	0.95
228_S	231_V	1.237	0.95
131_T	134_E	1.231	0.94
160_E	164_S	1.228	0.94
190_M	194_T	1.223	0.94
30_P	165_T	1.216	0.94
64_Q	162_E	1.215	0.94
211_S	220_P	1.215	0.94
39_D	280_F	1.213	0.94
92_P	242_C	1.190	0.93
136_L	154_Y	1.179	0.93
28_Y	32_L	1.177	0.93
185_D	188_A	1.164	0.93
71_S	143_I	1.162	0.93
91_E	246_N	1.160	0.92
47_S	215_L	1.155	0.92
213_F	235_C	1.151	0.92
122_V	181_I	1.144	0.92
25_V	37_K	1.132	0.92
51_C	207_A	1.131	0.91
12_L	296_L	1.125	0.91
212_V	219_H	1.119	0.91
53_S	204_L	1.116	0.91
275_F	306_L	1.106	0.91
101_I	176_M	1.104	0.90
26_A	32_L	1.101	0.90
71_S	124_T	1.101	0.90
121_L	189_V	1.094	0.90
60_T	182_F	1.093	0.90
8_E	303_M	1.092	0.90
129_G	134_E	1.091	0.90
206_L	278_G	1.090	0.90
30_P	161_S	1.083	0.90
257_I	270_I	1.081	0.90
267_I	290_S	1.080	0.89
29_I	162_E	1.076	0.89
71_S	196_Q	1.063	0.89
103_L	111_P	1.053	0.88
139_L	192_L	1.052	0.88
295_K	299_I	1.051	0.88
78_E	128_R	1.046	0.88
87_Y	183_E	1.035	0.87
47_S	211_S	1.028	0.87
170_R	187_E	1.028	0.87
46_Y	52_L	1.021	0.87
168_I	178_Q	1.019	0.87
121_L	190_M	1.016	0.86
66_I	206_L	1.015	0.86
144_R	147_T	1.012	0.86
250_E	254_K	1.005	0.86
29_I	161_S	0.983	0.85
129_G	135_R	0.982	0.84
10_E	14_K	0.981	0.84
159_A	163_Y	0.977	0.84
144_R	150_Q	0.974	0.84
208_K	212_V	0.970	0.84
209_I	213_F	0.967	0.84
212_V	226_V	0.961	0.83
184_D	189_V	0.956	0.83
37_K	283_A	0.952	0.83
13_V	17_K	0.950	0.82
87_Y	181_I	0.946	0.82
38_H	283_A	0.944	0.82
51_C	208_K	0.942	0.82
92_P	111_P	0.941	0.82
283_A	292_A	0.935	0.81
43_A	51_C	0.933	0.81
259_A	285_D	0.931	0.81
73_A	213_F	0.927	0.81
42_V	300_M	0.921	0.80
12_L	299_I	0.921	0.80
170_R	174_Y	0.914	0.80
71_S	127_I	0.906	0.79
93_T	112_L	0.905	0.79
144_R	152_I	0.898	0.79
270_I	276_G	0.897	0.79
85_F	234_I	0.890	0.78
67_S	71_S	0.886	0.78
234_I	253_I	0.879	0.77
47_S	214_A	0.876	0.77
8_E	304_Y	0.871	0.77
132_V	192_L	0.865	0.76
109_S	112_L	0.860	0.76
44_I	277_I	0.851	0.75
111_P	162_E	0.849	0.75
147_T	152_I	0.849	0.75
267_I	279_I	0.846	0.74
16_A	19_V	0.839	0.74
92_P	246_N	0.836	0.74
76_L	88_V	0.834	0.73
182_F	189_V	0.832	0.73
127_I	139_L	0.829	0.73
75_V	79_Y	0.825	0.73
225_Q	229_K	0.824	0.73
140_L	144_R	0.822	0.72
63_L	264_S	0.819	0.72
65_S	68_K	0.817	0.72
6_N	52_L	0.816	0.72
171_A	174_Y	0.813	0.71
237_T	307_S	0.811	0.71
147_T	209_I	0.808	0.71
29_I	33_A	0.798	0.70
252_A	271_S	0.792	0.70
116_I	242_C	0.791	0.69
130_R	134_E	0.789	0.69
91_E	242_C	0.788	0.69
41_S	57_V	0.788	0.69
91_E	94_G	0.785	0.69
250_E	266_G	0.784	0.69
150_Q	205_D	0.783	0.69
244_M	285_D	0.780	0.68
143_I	199_I	0.779	0.68
124_T	196_Q	0.775	0.68
154_Y	195_K	0.771	0.67
28_Y	161_S	0.769	0.67
158_V	161_S	0.768	0.67
23_G	32_L	0.768	0.67
137_D	140_L	0.761	0.66
122_V	125_S	0.761	0.66
103_L	112_L	0.759	0.66
13_V	296_L	0.758	0.66
60_T	151_E	0.755	0.66
271_S	274_D	0.752	0.65
131_T	135_R	0.748	0.65
92_P	103_L	0.747	0.65
25_V	33_A	0.747	0.65
62_T	280_F	0.745	0.65
240_V	309_F	0.744	0.65
45_Y	49_N	0.743	0.65
46_Y	213_F	0.742	0.64
42_V	279_I	0.741	0.64
62_T	159_A	0.740	0.64
141_S	145_R	0.738	0.64
227_I	232_A	0.737	0.64
188_A	191_D	0.734	0.64
153_T	200_E	0.733	0.64
9_L	248_S	0.727	0.63
230_D	302_E	0.725	0.63
12_L	16_A	0.724	0.63
32_L	161_S	0.724	0.63
104_E	109_S	0.724	0.63
37_K	281_G	0.723	0.62
240_V	308_I	0.718	0.62
65_S	245_Y	0.715	0.62
64_Q	198_A	0.713	0.61
117_N	169_N	0.712	0.61
68_K	245_Y	0.710	0.61
217_G	220_P	0.709	0.61
104_E	107_N	0.709	0.61
231_V	235_C	0.704	0.60
21_D	283_A	0.701	0.60
162_E	263_V	0.701	0.60
155_C	158_V	0.700	0.60
26_A	165_T	0.699	0.60
18_K	21_D	0.696	0.59
163_Y	190_M	0.695	0.59
83_K	134_E	0.693	0.59
222_T	238_F	0.692	0.59
215_L	220_P	0.691	0.59
176_M	182_F	0.690	0.59
56_D	280_F	0.689	0.59
9_L	13_V	0.688	0.59
140_L	151_E	0.685	0.58
25_V	283_A	0.681	0.58
247_A	294_V	0.681	0.58
100_I	172_M	0.680	0.58
117_N	260_K	0.678	0.58
272_P	281_G	0.678	0.58
129_G	138_Y	0.676	0.57
29_I	263_V	0.676	0.57
79_Y	126_L	0.673	0.57
31_A	158_V	0.672	0.57
75_V	255_V	0.671	0.57
251_F	255_V	0.666	0.56
167_M	187_E	0.665	0.56
98_N	116_I	0.665	0.56
132_V	135_R	0.665	0.56
146_L	227_I	0.664	0.56
168_I	179_Y	0.662	0.56
40_L	292_A	0.662	0.56
73_A	146_L	0.659	0.55
165_T	170_R	0.658	0.55
45_Y	208_K	0.658	0.55
17_K	56_D	0.657	0.55
170_R	190_M	0.653	0.55
296_L	300_M	0.648	0.54
148_N	211_S	0.647	0.54
26_A	162_E	0.642	0.53
32_L	62_T	0.640	0.53
236_K	270_I	0.639	0.53
263_V	288_G	0.638	0.53
241_T	252_A	0.636	0.53
141_S	151_E	0.633	0.52
92_P	252_A	0.633	0.52
63_L	108_P	0.632	0.52
7_D	14_K	0.632	0.52
291_I	294_V	0.632	0.52
245_Y	260_K	0.631	0.52
159_A	191_D	0.631	0.52
12_L	15_K	0.631	0.52
94_G	249_G	0.630	0.52
60_T	200_E	0.630	0.52
6_N	17_K	0.629	0.52
160_E	163_Y	0.626	0.52
255_V	301_S	0.625	0.52
73_A	227_I	0.624	0.51
260_K	263_V	0.623	0.51
16_A	295_K	0.623	0.51
162_E	198_A	0.621	0.51
95_D	241_T	0.618	0.51
11_A	18_K	0.618	0.51
76_L	231_V	0.617	0.51
13_V	292_A	0.614	0.50
218_R	222_T	0.613	0.50
159_A	198_A	0.613	0.50
124_T	139_L	0.611	0.50
182_F	196_Q	0.610	0.50
69_V	115_M	0.604	0.49
185_D	232_A	0.602	0.49
283_A	291_I	0.601	0.49
214_A	217_G	0.601	0.49
247_A	250_E	0.599	0.49
113_N	199_I	0.597	0.48
210_G	275_F	0.596	0.48
29_I	97_F	0.593	0.48
204_L	208_K	0.593	0.48
133_K	195_K	0.590	0.48
140_L	286_E	0.589	0.47
70_L	213_F	0.588	0.47
23_G	29_I	0.586	0.47
136_L	195_K	0.585	0.47
41_S	60_T	0.585	0.47
88_V	111_P	0.582	0.47
75_V	192_L	0.581	0.46
47_S	216_N	0.581	0.46
24_E	130_R	0.579	0.46
9_L	95_D	0.579	0.46
211_S	214_A	0.578	0.46
139_L	172_M	0.577	0.46
197_C	263_V	0.577	0.46
218_R	221_E	0.576	0.46
285_D	289_N	0.576	0.46
25_V	166_S	0.576	0.46
64_Q	263_V	0.575	0.46
267_I	293_G	0.574	0.46
45_Y	211_S	0.573	0.46
159_A	195_K	0.573	0.46
73_A	235_C	0.573	0.46
175_Y	248_S	0.573	0.46
105_T	175_Y	0.570	0.45
10_E	38_H	0.568	0.45
70_L	143_I	0.568	0.45
9_L	54_A	0.568	0.45
29_I	64_Q	0.568	0.45
217_G	226_V	0.567	0.45
44_I	271_S	0.566	0.45
115_M	244_M	0.565	0.45
227_I	231_V	0.565	0.45
234_I	264_S	0.565	0.45
173_C	182_F	0.563	0.44
94_G	246_N	0.563	0.44
67_S	98_N	0.562	0.44
25_V	194_T	0.560	0.44
74_L	127_I	0.560	0.44
152_I	235_C	0.559	0.44
298_E	302_E	0.559	0.44
47_S	208_K	0.559	0.44
92_P	116_I	0.559	0.44
152_I	274_D	0.558	0.44
10_E	22_K	0.557	0.44
211_S	215_L	0.556	0.44
44_I	275_F	0.555	0.44
23_G	162_E	0.555	0.44
134_E	138_Y	0.554	0.43
155_C	268_M	0.552	0.43
128_R	182_F	0.552	0.43
64_Q	168_I	0.551	0.43
74_L	142_F	0.551	0.43
123_V	126_L	0.551	0.43
15_K	303_M	0.550	0.43
175_Y	251_F	0.549	0.43
24_E	286_E	0.548	0.43
152_I	201_M	0.547	0.43
245_Y	263_V	0.547	0.43
73_A	231_V	0.545	0.42
238_F	246_N	0.543	0.42
25_V	186_V	0.543	0.42
64_Q	91_E	0.542	0.42
215_L	302_E	0.542	0.42
135_R	188_A	0.542	0.42
50_V	121_L	0.540	0.42
69_V	239_M	0.539	0.42
279_I	297_L	0.539	0.42
137_D	151_E	0.537	0.42
231_V	234_I	0.537	0.42
26_A	161_S	0.537	0.42
116_I	181_I	0.536	0.41
117_N	197_C	0.536	0.41
90_Q	253_I	0.535	0.41
62_T	115_M	0.535	0.41
173_C	186_V	0.534	0.41
31_A	164_S	0.534	0.41
65_S	117_N	0.534	0.41
9_L	44_I	0.533	0.41
72_L	235_C	0.532	0.41
36_D	223_G	0.531	0.41
268_M	278_G	0.531	0.41
108_P	235_C	0.529	0.41
56_D	59_K	0.529	0.41
127_I	207_A	0.529	0.41
57_V	60_T	0.528	0.41
18_K	22_K	0.526	0.40
242_C	297_L	0.525	0.40
69_V	152_I	0.525	0.40
139_L	147_T	0.524	0.40
217_G	222_T	0.524	0.40
34_K	204_L	0.523	0.40
206_L	266_G	0.522	0.40
78_E	129_G	0.521	0.40
10_E	137_D	0.521	0.40
85_F	264_S	0.519	0.40
97_F	260_K	0.517	0.39
159_A	298_E	0.517	0.39
11_A	15_K	0.517	0.39
59_K	202_N	0.517	0.39
237_T	240_V	0.517	0.39
145_R	226_V	0.515	0.39
17_K	86_S	0.514	0.39
73_A	194_T	0.514	0.39
174_Y	276_G	0.514	0.39
59_K	280_F	0.511	0.39
45_Y	214_A	0.510	0.39
92_P	241_T	0.510	0.39
97_F	117_N	0.510	0.39
237_T	253_I	0.509	0.38
11_A	14_K	0.508	0.38
24_E	160_E	0.508	0.38
166_S	246_N	0.507	0.38
228_S	270_I	0.506	0.38
147_T	150_Q	0.505	0.38
113_N	242_C	0.504	0.38
21_D	38_H	0.504	0.38
172_M	232_A	0.504	0.38
202_N	205_D	0.502	0.38
244_M	250_E	0.502	0.38
68_K	117_N	0.501	0.38
125_S	159_A	0.501	0.38
27_S	58_E	0.501	0.38
99_S	104_E	0.501	0.38
126_L	220_P	0.501	0.38
212_V	222_T	0.500	0.37

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
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HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2pbyA	3	0.945	100	-0.009	Contact Map
1u60A	4	0.9903	100	0.001	Contact Map
1mkiA	3	0.9579	100	0.006	Contact Map
4bqmA	3	0.9741	100	0.015	Contact Map
3uo9A	3	0.9871	100	0.017	Contact Map
3agdA	2	0.9871	100	0.042	Contact Map
1ghpA	1	0.754	98.6	0.915	Contact Map
4m3kA	1	0.7573	98.4	0.919	Contact Map
4ua6A	1	0.7702	98.1	0.925	Contact Map
1n9bA	1	0.7767	98	0.928	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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