GREMLIN Database
GANA - Arabinogalactan endo-beta-1,4-galactanase
UniProt: O07013 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 429 (345)
Sequences: 378 (278)
Seq/√Len: 15.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
66_E126_R5.0251.00
196_T233_T4.1011.00
318_Y331_D3.9711.00
65_L70_V3.9441.00
206_T251_N3.9051.00
287_K342_A3.7621.00
93_Y347_F3.7571.00
159_L167_K3.7551.00
173_K221_E3.4650.99
220_R254_D3.2530.99
199_F208_M2.8300.97
98_I136_A2.7640.96
238_A279_A2.6700.96
169_Y221_E2.6380.95
161_F207_K2.5830.95
140_Y172_T2.5390.94
100_N171_Y2.5240.94
93_Y133_K2.4560.93
128_T186_I2.4390.93
217_R221_E2.3630.92
336_V343_G2.3570.91
238_A276_V2.3200.91
81_D126_R2.2800.90
84_T130_N2.2430.89
57_A91_V2.2370.89
223_N226_I2.2360.89
168_L215_G2.2080.88
96_V136_A2.1780.88
71_T79_R2.1560.87
227_L287_K2.1270.86
104_D108_N2.1200.86
237_T240_R2.1040.86
159_L163_A2.0930.86
281_A339_T2.0310.84
302_G406_F1.9910.82
303_D307_N1.9790.82
195_T212_F1.9700.82
74_N78_K1.9410.81
336_V346_V1.9060.80
273_L290_V1.8930.79
80_Q85_T1.8800.79
87_K132_M1.8740.78
74_N80_Q1.8380.77
161_F165_K1.8320.77
246_E283_T1.8070.76
96_V120_A1.7980.75
121_I182_E1.7890.75
161_F211_L1.7620.74
127_A134_V1.7520.73
245_A258_F1.7370.73
83_F130_N1.7260.72
62_V66_E1.7240.72
67_N119_K1.7190.72
246_E284_Y1.7190.72
334_E338_N1.7160.72
165_K214_E1.7110.72
165_K210_Q1.7010.71
117_V171_Y1.6940.71
323_Q327_T1.6910.71
96_V124_G1.6840.70
85_T410_P1.6730.70
89_A415_Q1.6700.70
117_V178_K1.6640.69
73_Y79_R1.6630.69
128_T187_G1.6560.69
160_S163_A1.6280.68
249_S255_Y1.6250.67
169_Y173_K1.6050.66
59_V83_F1.5940.66
65_L354_I1.5840.65
216_S228_V1.5810.65
62_V83_F1.5660.64
189_V219_V1.5500.64
328_A332_V1.5380.63
65_L72_F1.5270.62
62_V82_I1.5240.62
161_F210_Q1.5020.61
242_S283_T1.5010.61
67_N114_N1.5000.61
234_N241_Y1.4960.61
242_S279_A1.4900.60
110_Y154_K1.4840.60
174_Q178_K1.4750.60
121_I178_K1.4630.59
265_F401_Q1.4390.58
152_V200_A1.4080.56
303_D399_D1.4070.56
235_P276_V1.4000.56
133_K347_F1.3730.54
265_F294_S1.3670.54
99_W146_D1.3500.53
121_I179_M1.3430.52
326_A330_R1.3330.52
152_V203_T1.3080.50
172_T218_A1.2870.49
302_G399_D1.2770.49
166_A170_E1.2770.49
307_N399_D1.2640.48
220_R228_V1.2580.48
171_Y191_V1.2570.48
276_V279_A1.2480.47
213_N230_L1.2430.47
317_P320_I1.2310.46
169_Y217_R1.2290.46
241_Y260_S1.2210.46
173_K177_Q1.2190.45
79_R271_Q1.2160.45
277_L339_T1.2070.45
216_S220_R1.2040.45
110_Y377_A1.2020.44
98_I373_G1.1930.44
196_T399_D1.1920.44
99_W350_E1.1840.43
242_S276_V1.1800.43
122_E179_M1.1780.43
235_P339_T1.1760.43
185_D226_I1.1720.43
121_I184_V1.1690.43
135_L229_A1.1680.43
117_V121_I1.1610.42
410_P414_L1.1490.42
401_Q417_F1.1480.41
133_K187_G1.1410.41
169_Y218_A1.1280.40
117_V179_M1.1250.40
107_G110_Y1.1220.40
112_G397_A1.1190.40
99_W147_P1.1170.40
63_I119_K1.1150.40
181_K221_E1.0970.39
146_D190_Q1.0900.38
91_V414_L1.0900.38
259_A291_A1.0880.38
72_F354_I1.0870.38
103_Y114_N1.0870.38
295_Y307_N1.0770.38
281_A288_V1.0770.38
97_R230_L1.0750.38
238_A242_S1.0720.37
253_V307_N1.0720.37
116_D280_V1.0700.37
322_V413_S1.0670.37
217_R254_D1.0640.37
172_T262_Y1.0640.37
125_K180_I1.0610.37
218_A222_T1.0590.37
59_V347_F1.0540.36
232_F245_A1.0530.36
215_G266_W1.0470.36
132_M187_G1.0470.36
213_N253_V1.0380.36
59_V93_Y1.0350.36
62_V126_R1.0300.35
302_G355_P1.0230.35
241_Y276_V1.0220.35
158_N163_A1.0190.35
171_Y175_S1.0140.34
128_T185_D1.0090.34
98_I117_V1.0090.34
115_N343_G1.0070.34
292_E349_W1.0070.34
82_I130_N1.0060.34
294_S308_T1.0060.34
296_T299_A1.0050.34
86_L284_Y1.0030.34
211_L295_Y0.9940.33
229_A289_M0.9940.33
244_I248_L0.9910.33
215_G228_V0.9890.33
57_A94_V0.9870.33
196_T234_N0.9860.33
145_A332_V0.9830.33
134_V347_F0.9810.33
188_M229_A0.9740.32
100_N167_K0.9700.32
319_P357_G0.9690.32
212_F232_F0.9680.32
238_A283_T0.9670.32
163_A167_K0.9610.32
377_A393_Y0.9600.32
247_T250_K0.9580.32
168_L211_L0.9550.32
232_F241_Y0.9480.31
107_G154_K0.9440.31
298_T353_W0.9410.31
234_N270_L0.9370.31
191_V219_V0.9340.30
71_T334_E0.9340.30
53_F219_V0.9340.30
400_N404_F0.9310.30
272_N276_V0.9290.30
288_V339_T0.9280.30
103_Y154_K0.9260.30
274_T338_N0.9230.30
231_H349_W0.9200.30
176_L218_A0.9200.30
138_F176_L0.9140.30
291_A294_S0.9140.30
127_A130_N0.9130.30
310_P314_Q0.9110.29
403_L414_L0.9100.29
135_L190_Q0.9100.29
205_W248_L0.9090.29
57_A397_A0.9080.29
221_E254_D0.9070.29
127_A329_V0.8950.29
140_Y218_A0.8940.29
111_G154_K0.8940.29
301_D304_G0.8900.28
281_A343_G0.8890.28
76_N158_N0.8890.28
290_V336_V0.8840.28
66_E81_D0.8820.28
239_G260_S0.8770.28
277_L281_A0.8710.28
115_N119_K0.8710.28
229_A344_L0.8700.28
205_W247_T0.8680.27
199_F253_V0.8680.27
230_L248_L0.8670.27
127_A187_G0.8610.27
94_V127_A0.8600.27
136_A185_D0.8590.27
253_V295_Y0.8570.27
97_R136_A0.8570.27
109_G114_N0.8560.27
403_L413_S0.8550.27
229_A291_A0.8540.27
322_V411_L0.8540.27
95_R292_E0.8440.26
93_Y187_G0.8440.26
350_E353_W0.8410.26
336_V339_T0.8370.26
399_D406_F0.8350.26
218_A329_V0.8330.26
74_N89_A0.8320.26
398_V404_F0.8280.26
93_Y188_M0.8270.26
133_K217_R0.8250.26
97_R115_N0.8240.26
125_K182_E0.8230.25
57_A351_P0.8230.25
144_W292_E0.8210.25
67_N78_K0.8190.25
113_G353_W0.8180.25
117_V136_A0.8150.25
247_T251_N0.8130.25
235_P350_E0.8130.25
261_S292_E0.8130.25
173_K217_R0.8120.25
138_F175_S0.8080.25
179_M205_W0.8080.25
115_N319_P0.8060.25
135_L188_M0.8030.25
66_E417_F0.8020.25
60_S146_D0.8010.25
143_F297_Y0.8000.25
182_E321_S0.7990.24
374_S377_A0.7980.24
172_T258_F0.7960.24
170_E173_K0.7920.24
82_I91_V0.7900.24
191_V299_A0.7880.24
139_H147_P0.7860.24
144_W231_H0.7840.24
233_T402_A0.7820.24
144_W261_S0.7790.24
183_G338_N0.7780.24
231_H292_E0.7770.24
76_N160_S0.7770.24
326_A416_V0.7720.23
171_Y178_K0.7680.23
173_K218_A0.7670.23
377_A394_G0.7660.23
71_T130_N0.7640.23
112_G154_K0.7640.23
331_D334_E0.7620.23
176_L222_T0.7610.23
113_G376_W0.7610.23
217_R243_F0.7600.23
414_L418_Q0.7590.23
261_S349_W0.7590.23
171_Y230_L0.7580.23
95_R349_W0.7580.23
137_D146_D0.7580.23
413_S417_F0.7570.23
329_V403_L0.7560.23
99_W139_H0.7560.23
233_T307_N0.7460.22
274_T335_A0.7460.22
132_M300_E0.7440.22
135_L351_P0.7440.22
273_L351_P0.7420.22
99_W190_Q0.7420.22
137_D147_P0.7390.22
281_A342_A0.7360.22
119_K132_M0.7330.22
111_G114_N0.7310.22
281_A336_V0.7300.22
412_P415_Q0.7270.22
308_T398_V0.7220.21
181_K214_E0.7200.21
152_V211_L0.7200.21
144_W349_W0.7200.21
197_G243_F0.7190.21
69_G266_W0.7180.21
99_W353_W0.7140.21
99_W115_N0.7140.21
123_I126_R0.7130.21
52_D72_F0.7120.21
213_N254_D0.7080.21
72_F342_A0.7070.21
103_Y110_Y0.7050.21
305_H392_W0.7040.21
93_Y295_Y0.7040.21
62_V148_A0.7040.21
121_I208_M0.7040.21
310_P315_T0.7020.21
74_N171_Y0.6990.21
99_W343_G0.6980.21
205_W243_F0.6960.20
394_G399_D0.6950.20
115_N412_P0.6940.20
72_F116_D0.6940.20
214_E217_R0.6930.20
255_Y286_K0.6900.20
59_V91_V0.6900.20
299_A310_P0.6890.20
235_P245_A0.6880.20
99_W395_G0.6860.20
235_P290_V0.6840.20
82_I352_A0.6820.20
125_K282_N0.6810.20
300_E316_L0.6800.20
278_K282_N0.6800.20
294_S298_T0.6800.20
276_V283_T0.6800.20
136_A259_A0.6770.20
164_K168_L0.6760.20
95_R146_D0.6750.20
62_V81_D0.6750.20
246_E279_A0.6740.20
124_G164_K0.6740.20
118_Q129_A0.6720.20
83_F195_T0.6720.20
135_L171_Y0.6700.20
376_W404_F0.6690.19
99_W137_D0.6620.19
162_E213_N0.6600.19
274_T321_S0.6570.19
122_E203_T0.6570.19
139_H231_H0.6560.19
278_K340_G0.6530.19
213_N216_S0.6530.19
76_N334_E0.6510.19
146_D149_K0.6490.19
291_A332_V0.6480.19
76_N227_L0.6480.19
96_V189_V0.6470.19
232_F396_S0.6460.19
405_D409_H0.6440.19
152_V164_K0.6440.19
279_A283_T0.6410.19
46_V323_Q0.6410.19
199_F215_G0.6400.18
121_I191_V0.6390.18
234_N332_V0.6380.18
160_S225_N0.6380.18
185_D227_L0.6370.18
87_K180_I0.6360.18
167_K171_Y0.6340.18
134_V184_V0.6330.18
230_L253_V0.6280.18
170_E221_E0.6260.18
84_T160_S0.6250.18
59_V75_T0.6230.18
80_Q84_T0.6230.18
260_S280_V0.6190.18
86_L290_V0.6180.18
144_W147_P0.6170.18
95_R231_H0.6160.18
113_G124_G0.6160.18
69_G356_V0.6150.18
175_S226_I0.6150.18
179_M186_I0.6120.18
190_Q294_S0.6110.18
94_V227_L0.6090.17
95_R147_P0.6080.17
164_K211_L0.6080.17
166_A169_Y0.6070.17
98_I265_F0.6050.17
259_A328_A0.6030.17
195_T277_L0.6030.17
97_R292_E0.6030.17
198_G201_G0.6000.17
196_T307_N0.5990.17
87_K277_L0.5990.17
76_N177_Q0.5980.17
143_F377_A0.5960.17
409_H412_P0.5960.17
138_F167_K0.5960.17
283_T396_S0.5960.17
206_T247_T0.5950.17
405_D411_L0.5940.17
291_A406_F0.5920.17
175_S236_E0.5920.17
99_W145_A0.5920.17
138_F189_V0.5910.17
267_H273_L0.5910.17
95_R261_S0.5910.17
295_Y320_I0.5910.17
311_K407_N0.5900.17
165_K321_S0.5900.17
306_G310_P0.5880.17
128_T184_V0.5870.17
164_K284_Y0.5870.17
100_N200_A0.5860.17
139_H144_W0.5860.17
147_P268_G0.5840.17
145_A375_G0.5830.17
114_N138_F0.5820.17
99_W144_W0.5820.17
139_H190_Q0.5800.17
92_N187_G0.5800.17
199_F209_C0.5800.17
136_A216_S0.5800.17
114_N215_G0.5780.16
170_E311_K0.5770.16
49_M52_D0.5770.16
257_V287_K0.5760.16
242_S246_E0.5750.16
91_V206_T0.5750.16
399_D402_A0.5730.16
356_V375_G0.5720.16
95_R144_W0.5720.16
218_A226_I0.5720.16
157_A166_A0.5700.16
140_Y215_G0.5700.16
191_V262_Y0.5700.16
72_F351_P0.5690.16
165_K314_Q0.5680.16
321_S416_V0.5660.16
137_D149_K0.5650.16
257_V260_S0.5650.16
169_Y214_E0.5650.16
191_V228_V0.5620.16
64_A154_K0.5620.16
100_N340_G0.5610.16
145_A291_A0.5600.16
61_S352_A0.5600.16
111_G378_S0.5580.16
126_R284_Y0.5560.16
68_S374_S0.5530.16
273_L336_V0.5500.16
131_G176_L0.5460.16
139_H292_E0.5460.16
108_N409_H0.5450.15
158_N285_G0.5450.15
93_Y132_M0.5430.15
319_P328_A0.5430.15
161_F203_T0.5420.15
227_L257_V0.5410.15
160_S179_M0.5410.15
343_G353_W0.5380.15
92_N119_K0.5380.15
177_Q181_K0.5370.15
305_H397_A0.5360.15
86_L198_G0.5360.15
322_V416_V0.5350.15
294_S374_S0.5350.15
101_H415_Q0.5330.15
198_G292_E0.5330.15
176_L248_L0.5310.15
63_I122_E0.5310.15
61_S65_L0.5300.15
91_V94_V0.5300.15
118_Q180_I0.5290.15
93_Y121_I0.5290.15
71_T378_S0.5270.15
147_P190_Q0.5270.15
301_D307_N0.5260.15
196_T402_A0.5260.15
233_T303_D0.5230.15
171_Y215_G0.5230.15
148_A397_A0.5200.15
180_I226_I0.5200.15
96_V123_I0.5190.15
97_R349_W0.5190.15
296_T309_A0.5190.15
196_T240_R0.5180.15
129_A403_L0.5170.15
137_D268_G0.5160.15
350_E356_V0.5160.15
64_A258_F0.5160.15
59_V159_L0.5130.15
217_R252_K0.5130.15
190_Q347_F0.5130.15
98_I229_A0.5120.15
61_S374_S0.5110.15
214_E322_V0.5110.15
60_S99_W0.5100.14
121_I175_S0.5090.14
162_E210_Q0.5090.14
120_A179_M0.5090.14
231_H261_S0.5080.14
166_A228_V0.5070.14
236_E267_H0.5070.14
88_Q174_Q0.5060.14
95_R139_H0.5050.14
257_V281_A0.5050.14
176_L224_S0.5050.14
216_S299_A0.5030.14
81_D357_G0.5020.14
207_K237_T0.5010.14
97_R408_G0.5000.14
145_A190_Q0.5000.14
87_K166_A0.5000.14
213_N407_N0.5000.14
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1ur4A 1 0.9021 100 0.544 Contact Map
1fobA 1 0.7483 100 0.653 Contact Map
1hjsA 1 0.7459 100 0.657 Contact Map
4cd8A 1 0.697 100 0.812 Contact Map
4k68A 1 0.7413 100 0.819 Contact Map
4v2xA 2 0.7925 100 0.82 Contact Map
3ndzA 3 0.7296 100 0.828 Contact Map
1ur1A 1 0.7319 100 0.83 Contact Map
4pmuA 2 0.7529 100 0.831 Contact Map
4w8bA 1 0.7506 100 0.832 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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