GREMLIN Database
TSAE - tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
UniProt: O05515 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 158 (136)
Sequences: 4089 (2961)
Seq/√Len: 253.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
34_E121_Q2.5201.00
8_T14_T2.5051.00
45_T104_L2.3951.00
25_A48_F2.3081.00
10_N13_E2.2751.00
44_F48_F2.1571.00
9_V13_E2.1041.00
125_K133_E2.0641.00
48_F104_L2.0611.00
34_E109_H2.0251.00
125_K135_T2.0181.00
17_I134_I1.9961.00
127_A133_E1.9801.00
18_A44_F1.9451.00
34_E123_V1.9071.00
124_I134_I1.8911.00
33_L45_T1.8891.00
15_K50_E1.8051.00
15_K47_G1.7961.00
68_E77_Y1.7691.00
49_A76_L1.7681.00
19_K50_E1.7261.00
77_Y101_G1.6961.00
49_A58_V1.6771.00
32_T108_A1.6721.00
15_K46_K1.6671.00
92_G95_E1.6321.00
25_A31_L1.6101.00
81_V111_I1.5971.00
8_T134_I1.5861.00
59_N67_K1.5861.00
121_Q137_T1.5711.00
21_T44_F1.5491.00
34_E108_A1.5471.00
123_V135_T1.5351.00
18_A47_G1.4881.00
123_V137_T1.4421.00
11_P15_K1.4381.00
11_P47_G1.4141.00
26_K29_D1.4071.00
16_A20_L1.3881.00
8_T17_I1.3861.00
79_M93_L1.3781.00
127_A131_E1.3721.00
9_V131_E1.3651.00
88_S93_L1.3371.00
32_T119_R1.3371.00
7_R131_E1.3301.00
54_I58_V1.3171.00
47_G51_G1.3121.00
97_F103_C1.2971.00
49_A54_I1.2371.00
8_T13_E1.2181.00
44_F124_I1.2161.00
17_I136_F1.2051.00
7_R133_E1.2031.00
19_K23_A1.1741.00
22_A52_L1.1671.00
89_E94_D1.1671.00
81_V108_A1.1641.00
7_R127_A1.1381.00
85_E110_L1.1341.00
11_P43_T1.1341.00
66_I79_M1.1131.00
22_A51_G1.1091.00
71_D74_L1.1051.00
31_L104_L1.0961.00
74_L102_V1.0901.00
16_A19_K1.0821.00
119_R140_G1.0781.00
121_Q139_V1.0541.00
36_D126_R1.0501.00
19_K51_G1.0301.00
68_E96_Y1.0031.00
37_L82_Y0.9971.00
79_M103_C0.9891.00
76_L104_L0.9811.00
70_N75_P0.9791.00
75_P101_G0.9781.00
32_T121_Q0.9771.00
93_L97_F0.9701.00
14_T18_A0.9611.00
88_S111_I0.9571.00
97_F105_V0.9291.00
46_K57_I0.9121.00
115_L119_R0.9061.00
79_M96_Y0.9051.00
48_F102_V0.9021.00
20_L24_F0.8911.00
25_A29_D0.8811.00
77_Y103_C0.8781.00
87_E110_L0.8731.00
66_I77_Y0.8671.00
120_L138_A0.8671.00
53_G56_R0.8641.00
30_V105_V0.8630.99
14_T134_I0.8620.99
66_I96_Y0.8570.99
20_L23_A0.8540.99
18_A22_A0.8500.99
75_P99_G0.8390.99
14_T124_I0.8310.99
94_D98_H0.8180.99
88_S91_L0.8150.99
54_I76_L0.8140.99
31_L120_L0.8130.99
29_D102_V0.8110.99
21_T120_L0.8100.99
22_A48_F0.8090.99
128_G131_E0.8030.99
31_L122_I0.8000.99
14_T43_T0.7840.99
84_M88_S0.7830.99
45_T49_A0.7790.99
11_P46_K0.7690.99
68_E75_P0.7680.99
12_E16_A0.7640.99
21_T122_I0.7580.99
12_E15_K0.7570.99
87_E90_D0.7500.99
59_N65_I0.7460.99
13_E16_A0.7380.98
88_S114_Q0.7270.98
81_V110_L0.7170.98
66_I91_L0.7170.98
33_L104_L0.7130.98
32_T111_I0.7100.98
11_P18_A0.7080.98
71_D75_P0.7020.98
15_K19_K0.6990.98
8_T124_I0.6980.98
127_A130_D0.6930.98
72_G75_P0.6910.98
25_A102_V0.6890.98
32_T118_E0.6800.97
18_A48_F0.6740.97
39_A126_R0.6630.97
46_K49_A0.6610.97
59_N64_T0.6580.97
74_L101_G0.6580.97
45_T48_F0.6520.97
77_Y96_Y0.6510.97
14_T17_I0.6480.96
81_V105_V0.6440.96
76_L102_V0.6410.96
111_I115_L0.6400.96
84_M110_L0.6400.96
21_T48_F0.6370.96
32_T63_F0.6370.96
122_I136_F0.6350.96
84_M93_L0.6340.96
84_M91_L0.6340.96
119_R139_V0.6210.96
6_W20_L0.6090.95
87_E92_G0.6090.95
8_T43_T0.6080.95
87_E91_L0.6070.95
15_K43_T0.6040.95
27_P74_L0.6020.95
43_T46_K0.6020.95
64_T69_Y0.6000.95
116_P119_R0.5940.94
53_G73_V0.5920.94
81_V88_S0.5910.94
45_T76_L0.5890.94
28_G100_Q0.5880.94
17_I21_T0.5850.94
91_L96_Y0.5850.94
44_F47_G0.5790.94
66_I92_G0.5790.94
36_D125_K0.5770.93
64_T67_K0.5740.93
14_T47_G0.5720.93
63_F118_E0.5670.93
18_A51_G0.5660.93
122_I134_I0.5590.92
81_V84_M0.5590.92
46_K50_E0.5560.92
8_T47_G0.5490.92
51_G73_V0.5460.91
85_E114_Q0.5430.91
19_K47_G0.5420.91
79_M116_P0.5390.91
57_I67_K0.5380.91
75_P100_Q0.5370.91
36_D39_A0.5360.91
70_N74_L0.5340.90
109_H123_V0.5330.90
39_A128_G0.5320.90
55_T129_D0.5260.90
21_T51_G0.5260.90
120_L136_F0.5250.90
79_M92_G0.5240.90
49_A67_K0.5230.90
52_L102_V0.5230.90
105_V115_L0.5220.89
118_E140_G0.5160.89
22_A25_A0.5140.89
7_R10_N0.5130.89
23_A141_N0.5130.89
91_L94_D0.5070.88
92_G104_L0.5050.88
31_L48_F0.5010.88
8_T11_P0.5000.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1htwA 1 0.9557 100 0.323 Contact Map
1tueA 1 0.962 97.7 0.838 Contact Map
1svmA 3 0.981 97.3 0.848 Contact Map
2gnoA 1 0.8481 97.3 0.849 Contact Map
3tmkA 2 0.8165 97.3 0.849 Contact Map
2p5tB 1 0.9051 97.3 0.849 Contact Map
2c95A 1 0.8354 97.3 0.85 Contact Map
1a7jA 5 0.8228 97.2 0.85 Contact Map
2v54A 2 0.8101 97.2 0.851 Contact Map
2yogA 1 0.7658 97.1 0.853 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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