GREMLIN Database
MAEN - Na(+)-malate symporter
UniProt: O05256 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 448 (421)
Sequences: 381 (252)
Seq/√Len: 12.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
99_F103_Y4.9931.00
287_F313_V4.5431.00
75_D79_R4.1481.00
95_F228_V4.0691.00
236_L240_L4.0581.00
167_I177_A3.8760.99
127_Y339_T3.6000.99
169_V370_S3.2940.98
227_N403_L3.2850.98
155_L400_H3.1060.97
400_H428_T2.9880.96
124_L194_G2.9840.96
170_G180_S2.9730.96
229_F369_I2.8810.95
395_I415_S2.7450.93
166_G185_V2.7370.93
409_V421_M2.7130.93
291_G306_M2.6410.92
118_M345_G2.5460.90
188_I226_G2.5230.90
332_K336_S2.5210.90
238_K242_D2.4530.89
376_I394_A2.4520.89
278_G337_S2.3100.85
245_P248_N2.2840.85
295_E302_G2.2530.84
106_L284_A2.2150.83
115_T349_L2.0790.78
293_L308_I2.0550.78
314_K324_E2.0410.77
56_P61_G2.0270.77
220_V225_I2.0250.77
248_N392_E2.0150.76
164_A366_I2.0130.76
374_A378_S1.9940.75
126_F430_L1.9900.75
395_I418_M1.9700.74
123_F342_L1.9690.74
147_G423_F1.9480.74
308_I312_A1.9410.73
221_P314_K1.9380.73
222_A403_L1.9180.72
293_L297_F1.9070.72
197_I223_A1.8930.71
159_T375_M1.8830.71
217_S430_L1.8820.71
117_L343_M1.8780.71
190_A428_T1.8730.71
139_M314_K1.8700.70
314_K422_P1.8610.70
44_V373_I1.8490.69
159_T400_H1.8280.69
339_T406_T1.8200.68
167_I185_V1.8160.68
348_I351_I1.8080.68
237_M351_I1.7990.67
297_F413_S1.7930.67
59_M127_Y1.7830.67
284_A394_A1.7770.66
278_G334_I1.7690.66
108_S112_D1.7680.66
99_F357_A1.7680.66
225_I368_C1.7440.65
301_P428_T1.7320.64
44_V312_A1.7270.64
76_I156_V1.7250.64
30_E446_F1.7070.63
126_F299_F1.7060.63
374_A402_G1.6980.63
245_P250_N1.6790.62
135_S291_G1.6570.61
171_F177_A1.6500.60
347_G399_C1.6470.60
94_L351_I1.6140.59
73_L96_V1.6130.58
282_L415_S1.5820.57
375_M400_H1.5820.57
357_A427_S1.5640.56
151_M155_L1.5580.56
203_A436_V1.5530.55
250_N419_G1.5520.55
59_M288_F1.5470.55
74_G340_W1.5440.55
133_V430_L1.5280.54
343_M371_V1.5270.54
103_Y353_L1.5080.53
165_A374_A1.4960.53
415_S419_G1.4840.52
34_I160_I1.4640.51
221_P423_F1.4630.51
84_K446_F1.4580.51
189_I197_I1.4440.50
277_M326_G1.4320.49
349_L406_T1.4310.49
373_I381_F1.4270.49
184_V314_K1.4130.48
401_S428_T1.4110.48
182_F436_V1.4100.48
44_V164_A1.3880.47
161_A374_A1.3800.47
150_R184_V1.3790.47
167_I181_F1.3540.45
83_L218_Q1.3490.45
40_T76_I1.3470.45
44_V168_L1.3330.44
124_L425_Q1.3230.44
53_N109_T1.3150.43
411_I428_T1.3060.43
384_K390_P1.3060.43
93_S197_I1.3040.43
281_V338_F1.2980.43
130_C330_L1.2790.42
173_F363_P1.2740.41
338_F437_I1.2620.41
290_F309_S1.2600.41
312_A394_A1.2550.40
59_M345_G1.2520.40
241_G245_P1.2520.40
98_S352_P1.2470.40
246_D250_N1.2460.40
53_N318_I1.2400.40
237_M391_V1.2380.40
151_M402_G1.2370.40
401_S408_D1.2340.39
114_V342_L1.2340.39
330_L392_E1.2290.39
210_S214_V1.2220.39
222_A341_P1.2130.38
173_F176_S1.2120.38
100_L105_V1.2060.38
386_M446_F1.1970.38
316_A327_A1.1950.38
83_L92_L1.1930.37
240_L247_L1.1920.37
24_Q427_S1.1820.37
220_V392_E1.1820.37
335_S343_M1.1810.37
411_I436_V1.1790.37
195_E303_A1.1760.37
410_A425_Q1.1710.36
426_I430_L1.1690.36
84_K208_L1.1640.36
216_V423_F1.1630.36
88_G425_Q1.1630.36
47_I51_Y1.1610.36
39_Y72_F1.1590.36
259_A416_G1.1580.36
248_N334_I1.1570.36
400_H411_I1.1540.36
94_L117_L1.1490.35
126_F281_V1.1460.35
30_E361_S1.1440.35
339_T342_L1.1380.35
193_I388_M1.1380.35
31_I225_I1.1310.35
197_I241_G1.1240.34
302_G352_P1.1160.34
329_Q337_S1.1140.34
217_S313_V1.1090.34
30_E76_I1.1060.33
89_P342_L1.1040.33
199_P425_Q1.0950.33
80_I241_G1.0940.33
65_I288_F1.0930.33
88_G131_L1.0910.33
237_M248_N1.0850.32
278_G307_I1.0840.32
132_V201_S1.0760.32
409_V433_A1.0760.32
39_Y392_E1.0760.32
189_I194_G1.0730.32
63_F76_I1.0710.32
114_V232_I1.0700.32
150_R314_K1.0700.32
205_S212_A1.0650.32
305_L440_T1.0520.31
293_L361_S1.0480.31
31_I36_L1.0480.31
107_N189_I1.0430.31
266_K373_I1.0410.31
188_I427_S1.0370.30
95_F206_Q1.0360.30
46_I391_V1.0340.30
76_I80_I1.0320.30
369_I373_I1.0300.30
151_M428_T1.0300.30
233_C373_I1.0300.30
119_K350_F1.0270.30
47_I100_L1.0250.30
184_V222_A1.0230.30
128_I306_M1.0210.30
226_G406_T1.0170.30
155_L428_T1.0160.29
91_I441_V1.0150.29
40_T160_I1.0150.29
159_T428_T1.0130.29
237_M241_G1.0110.29
206_Q381_F1.0040.29
30_E96_V1.0040.29
148_F154_P1.0030.29
378_S402_G1.0020.29
97_P304_I1.0000.29
44_V210_S1.0000.29
162_A230_A0.9990.29
413_S436_V0.9970.29
156_V274_F0.9950.29
34_I167_I0.9940.29
295_E312_A0.9900.28
139_M423_F0.9870.28
132_V426_I0.9810.28
31_I299_F0.9810.28
43_A91_I0.9800.28
221_P410_A0.9800.28
95_F216_V0.9780.28
325_E386_M0.9770.28
193_I346_L0.9750.28
301_P401_S0.9730.28
188_I408_D0.9730.28
160_I258_K0.9710.28
304_I411_I0.9700.28
158_G444_R0.9690.28
80_I366_I0.9690.28
34_I53_N0.9680.28
56_P345_G0.9680.28
318_I392_E0.9670.27
421_M433_A0.9670.27
190_A364_F0.9640.27
226_G425_Q0.9630.27
422_P426_I0.9620.27
335_S437_I0.9580.27
169_V430_L0.9570.27
335_S361_S0.9570.27
298_I365_V0.9560.27
69_L143_V0.9520.27
201_S209_G0.9470.27
46_I50_A0.9470.27
237_M364_F0.9440.27
99_F360_I0.9400.26
180_S184_V0.9370.26
313_V319_L0.9340.26
240_L244_R0.9330.26
298_I435_T0.9320.26
231_I392_E0.9300.26
114_V209_G0.9280.26
283_L366_I0.9230.26
100_L326_G0.9220.26
253_L426_I0.9180.26
138_G307_I0.9180.26
408_D428_T0.9180.26
170_G410_A0.9170.25
214_V247_L0.9160.25
306_M333_F0.9130.25
230_A351_I0.9120.25
180_S310_A0.9100.25
220_V413_S0.9100.25
400_H408_D0.9080.25
139_M234_A0.9080.25
212_A356_V0.9070.25
304_I436_V0.9040.25
301_P436_V0.9020.25
350_F356_V0.8990.25
294_L305_L0.8990.25
141_R145_I0.8940.25
400_H427_S0.8940.25
117_L193_I0.8930.25
48_L286_T0.8880.24
61_G118_M0.8870.24
204_Y234_A0.8860.24
126_F278_G0.8840.24
317_N324_E0.8820.24
45_I137_L0.8810.24
122_N125_Y0.8810.24
427_S430_L0.8750.24
157_A378_S0.8740.24
74_G367_I0.8740.24
303_A436_V0.8720.24
211_S297_F0.8700.24
97_P154_P0.8700.24
89_P153_V0.8680.24
312_A444_R0.8680.24
236_L268_A0.8670.24
250_N348_I0.8670.24
154_P379_G0.8660.24
75_D371_V0.8650.24
65_I298_I0.8640.23
345_G430_L0.8630.23
259_A389_Y0.8620.23
104_N419_G0.8620.23
140_N319_L0.8560.23
223_A388_M0.8540.23
92_L226_G0.8540.23
168_L287_F0.8520.23
319_L324_E0.8510.23
360_I393_S0.8490.23
193_I324_E0.8470.23
100_L214_V0.8470.23
196_G199_P0.8430.23
37_P41_V0.8430.23
255_K308_I0.8420.23
96_V394_A0.8410.23
70_G93_S0.8380.23
168_L172_I0.8370.23
209_G308_I0.8360.22
233_C376_I0.8350.22
405_G409_V0.8350.22
258_K266_K0.8310.22
164_A309_S0.8310.22
156_V164_A0.8300.22
266_K323_M0.8300.22
140_N277_M0.8260.22
181_F416_G0.8260.22
426_I435_T0.8230.22
158_G379_G0.8210.22
116_N236_L0.8140.22
200_L213_D0.8130.22
375_M428_T0.8070.21
223_A403_L0.8070.21
53_N105_V0.8050.21
168_L171_F0.8050.21
290_F294_L0.8040.21
313_V361_S0.8000.21
226_G235_A0.7990.21
155_L401_S0.7930.21
281_V307_I0.7930.21
97_P411_I0.7930.21
357_A361_S0.7930.21
35_P38_L0.7900.21
59_M354_D0.7890.21
220_V437_I0.7870.21
307_I341_P0.7860.21
186_V436_V0.7850.21
143_V151_M0.7850.21
114_V435_T0.7850.21
236_L318_I0.7840.21
128_I194_G0.7840.21
94_L343_M0.7830.21
44_V48_L0.7830.21
88_G227_N0.7830.21
173_F244_R0.7830.21
195_E436_V0.7800.21
408_D427_S0.7780.21
273_D307_I0.7780.21
375_M411_I0.7780.21
112_D357_A0.7740.20
74_G97_P0.7730.20
63_F242_D0.7720.20
126_F213_D0.7710.20
106_L348_I0.7710.20
216_V403_L0.7710.20
226_G403_L0.7690.20
193_I423_F0.7670.20
106_L409_V0.7670.20
47_I255_K0.7660.20
102_F354_D0.7660.20
141_R244_R0.7650.20
208_L225_I0.7650.20
172_I180_S0.7630.20
164_A168_L0.7630.20
203_A301_P0.7620.20
319_L382_V0.7620.20
237_M408_D0.7610.20
172_I243_K0.7610.20
373_I439_A0.7600.20
70_G231_I0.7600.20
224_I403_L0.7600.20
41_V313_V0.7580.20
221_P443_L0.7570.20
165_A378_S0.7560.20
114_V415_S0.7550.20
184_V219_L0.7550.20
117_L216_V0.7520.20
195_E402_G0.7520.20
39_Y58_N0.7470.19
346_L403_L0.7460.19
377_G446_F0.7440.19
140_N406_T0.7440.19
307_I440_T0.7440.19
342_L409_V0.7430.19
175_Y413_S0.7400.19
133_V306_M0.7390.19
180_S226_G0.7340.19
307_I410_A0.7320.19
362_I366_I0.7290.19
132_V231_I0.7280.19
399_C424_A0.7280.19
85_D215_F0.7270.19
216_V221_P0.7260.19
258_K262_I0.7250.19
177_A367_I0.7240.19
80_I211_S0.7220.19
303_A380_Y0.7210.19
59_M361_S0.7200.19
273_D370_S0.7190.19
45_I212_A0.7190.19
96_V304_I0.7190.19
157_A257_K0.7180.19
94_L403_L0.7180.19
230_A237_M0.7180.19
112_D171_F0.7160.19
132_V287_F0.7160.19
293_L298_I0.7140.18
307_I338_F0.7140.18
131_L427_S0.7140.18
73_L164_A0.7130.18
248_N295_E0.7130.18
58_N283_L0.7100.18
69_L76_I0.7080.18
221_P403_L0.7070.18
342_L433_A0.7070.18
43_A282_L0.7060.18
93_S349_L0.7050.18
105_V176_S0.7040.18
66_I346_L0.7020.18
254_V335_S0.7000.18
61_G347_G0.6980.18
56_P280_G0.6970.18
258_K389_Y0.6950.18
348_I442_L0.6950.18
246_D372_V0.6950.18
145_I204_Y0.6950.18
352_P396_V0.6900.18
61_G230_A0.6890.18
168_L323_M0.6880.18
35_P48_L0.6880.18
208_L293_L0.6880.18
219_L314_K0.6880.18
64_A282_L0.6860.18
116_N393_S0.6860.18
241_G391_V0.6850.18
380_Y411_I0.6850.18
281_V413_S0.6830.18
248_N351_I0.6820.18
46_I90_A0.6810.17
276_L319_L0.6800.17
271_K446_F0.6790.17
55_L231_I0.6790.17
195_E397_T0.6780.17
401_S427_S0.6760.17
203_A411_I0.6750.17
131_L307_I0.6740.17
92_L305_L0.6720.17
184_V413_S0.6720.17
104_N177_A0.6720.17
238_K381_F0.6720.17
94_L222_A0.6720.17
197_I216_V0.6700.17
193_I221_P0.6690.17
407_G432_G0.6680.17
47_I434_G0.6670.17
151_M400_H0.6620.17
190_A215_F0.6600.17
193_I216_V0.6590.17
143_V377_G0.6590.17
421_M425_Q0.6590.17
157_A400_H0.6590.17
363_P367_I0.6550.17
114_V171_F0.6540.17
41_V317_N0.6530.17
299_F444_R0.6520.17
38_L162_A0.6520.17
209_G215_F0.6520.17
387_N438_C0.6510.17
109_T394_A0.6490.17
44_V377_G0.6490.17
108_S209_G0.6490.17
157_A375_M0.6480.17
351_I391_V0.6480.17
152_F188_I0.6480.17
126_F334_I0.6400.16
151_M427_S0.6400.16
312_A316_A0.6380.16
98_S415_S0.6350.16
200_L436_V0.6350.16
195_E411_I0.6350.16
344_V440_T0.6350.16
97_P224_I0.6350.16
207_I443_L0.6330.16
26_I283_L0.6320.16
170_G414_A0.6310.16
314_K343_M0.6280.16
39_Y91_I0.6280.16
365_V392_E0.6270.16
217_S380_Y0.6240.16
98_S411_I0.6230.16
66_I350_F0.6230.16
40_T245_P0.6220.16
194_G425_Q0.6220.16
71_V340_W0.6220.16
288_F395_I0.6220.16
80_I92_L0.6210.16
49_A344_V0.6210.16
390_P424_A0.6190.16
199_P414_A0.6190.16
33_V153_V0.6190.16
55_L259_A0.6180.16
26_I165_A0.6170.16
402_G408_D0.6160.16
269_E286_T0.6150.16
152_F323_M0.6130.16
280_G383_G0.6130.16
227_N327_A0.6120.16
162_A408_D0.6110.16
73_L280_G0.6100.15
250_N286_T0.6080.15
150_R288_F0.6080.15
150_R414_A0.6080.15
412_L431_G0.6060.15
283_L324_E0.6060.15
307_I334_I0.6010.15
98_S428_T0.6010.15
159_T351_I0.6000.15
59_M384_K0.6000.15
65_I155_L0.5980.15
409_V423_F0.5970.15
301_P400_H0.5960.15
151_M401_S0.5960.15
206_Q298_I0.5950.15
255_K287_F0.5940.15
55_L70_G0.5940.15
188_I284_A0.5930.15
295_E299_F0.5930.15
139_M410_A0.5910.15
221_P425_Q0.5900.15
188_I439_A0.5890.15
152_F317_N0.5890.15
97_P402_G0.5880.15
294_L392_E0.5880.15
375_M396_V0.5870.15
101_V125_Y0.5870.15
146_Q319_L0.5870.15
287_F300_I0.5870.15
110_S362_I0.5850.15
211_S236_L0.5840.15
180_S323_M0.5820.15
131_L300_I0.5820.15
144_L395_I0.5820.15
127_Y409_V0.5810.15
35_P102_F0.5800.15
45_I228_V0.5790.15
339_T409_V0.5790.15
375_M427_S0.5770.15
380_Y383_G0.5740.15
226_G433_A0.5740.15
80_I446_F0.5740.15
34_I51_Y0.5730.15
53_N385_L0.5720.15
88_G397_T0.5720.15
139_M221_P0.5720.15
147_G324_E0.5710.14
133_V327_A0.5700.14
151_M396_V0.5700.14
353_L411_I0.5670.14
58_N122_N0.5670.14
73_L76_I0.5660.14
157_A385_L0.5660.14
408_D431_G0.5650.14
105_V115_T0.5650.14
222_A226_G0.5650.14
125_Y300_I0.5620.14
353_L371_V0.5620.14
120_T311_A0.5620.14
342_L350_F0.5610.14
42_L289_I0.5600.14
106_L444_R0.5600.14
185_V436_V0.5580.14
197_I401_S0.5580.14
42_L277_M0.5580.14
190_A436_V0.5580.14
396_V428_T0.5570.14
50_A64_A0.5570.14
111_L240_L0.5540.14
30_E271_K0.5540.14
200_L437_I0.5520.14
72_F113_A0.5510.14
294_L309_S0.5490.14
405_G425_Q0.5490.14
104_N318_I0.5470.14
48_L312_A0.5460.14
300_I384_K0.5460.14
165_A370_S0.5420.14
40_T44_V0.5400.14
48_L393_S0.5390.14
167_I171_F0.5390.14
410_A423_F0.5370.14
30_E69_L0.5360.14
40_T156_V0.5340.14
326_G391_V0.5340.14
224_I346_L0.5330.14
90_A390_P0.5310.13
89_P212_A0.5310.13
159_T401_S0.5310.13
138_G278_G0.5310.13
92_L274_F0.5290.13
320_P391_V0.5290.13
26_I36_L0.5280.13
319_L386_M0.5260.13
157_A389_Y0.5260.13
125_Y305_L0.5260.13
219_L371_V0.5250.13
231_I249_G0.5250.13
339_T433_A0.5230.13
66_I347_G0.5230.13
58_N248_N0.5220.13
98_S226_G0.5220.13
45_I156_V0.5210.13
202_I301_P0.5210.13
62_G113_A0.5200.13
133_V217_S0.5200.13
250_N384_K0.5190.13
125_Y352_P0.5150.13
240_L441_V0.5150.13
82_I156_V0.5140.13
189_I431_G0.5140.13
194_G342_L0.5130.13
70_G392_E0.5130.13
159_T411_I0.5130.13
56_P60_L0.5120.13
230_A402_G0.5110.13
73_L366_I0.5100.13
123_F350_F0.5090.13
224_I368_C0.5090.13
146_Q151_M0.5080.13
195_E301_P0.5070.13
44_V236_L0.5070.13
74_G146_Q0.5070.13
195_E352_P0.5060.13
97_P195_E0.5050.13
168_L355_D0.5050.13
24_Q393_S0.5040.13
84_K396_V0.5030.13
193_I403_L0.5010.13
63_F176_S0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4bwzA 2 0.7812 95.8 0.96 Contact Map
4czbA 2 0.3728 90.1 0.968 Contact Map
4cz8A 2 0.3661 84.9 0.97 Contact Map
3zuxA 1 0.4397 49.9 0.977 Contact Map
1zcdA 1 0.7299 48.5 0.977 Contact Map
4r0cA 3 0.8214 38.5 0.979 Contact Map
4f35D 2 0.8214 35.8 0.979 Contact Map
3qe7A 2 0.6652 19.4 0.982 Contact Map
4r1iA 2 0.8638 16.6 0.982 Contact Map
2ksfA 1 0.2388 9.5 0.984 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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