YUFN - BACSU - GREMLIN Contacts

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YUFN - Uncharacterized lipoprotein YufN

UniProt:	O05252	Sequences: MNKRKIGLAMSLVIAAGTILGACGNSEKSSGSGEGKNKFSVAMVTDVGGVDDKSFNQSAWEGIQAFGKENGLKKGKNGYDYLQSKSDADYTTNLNKLARENFDLIYGVGYLMEDSISEIADQRKNTNFAIIDAVVDKDNVASITFKEQEGSFLVGVAAALSSKSGKIGFVGGMESELIKKFEVGFRAGVQAVNPKAVVEVKYAGGFDKADVGKATAESMYKSGVDVIYHSAGATGTGVFTEAKNLKKEDPKRDVWVIGVDKDQYAEGQVEGTDDNVTLTSMVKKVDTVVEDVTKKASDGKFPGGETLTYGLDQDGVGISPSKQNLSDDVIKAVDKWKKKIIDGLEIPATEKELKTFKAE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	359 (315)
Sequences:	3738 (2764)
Seq/√Len:	155.8

BACSU - YUFN MED
HALSA - Q9HR20 Q9HRA0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
152_F	191_A	3.885	1.00
338_K	342_D	3.672	1.00
107_G	116_I	3.599	1.00
327_D	331_K	3.506	1.00
189_V	196_A	3.504	1.00
335_K	339_K	3.493	1.00
294_K	298_D	3.417	1.00
220_Y	254_V	3.065	1.00
165_G	193_N	2.821	1.00
91_T	118_E	2.752	1.00
156_V	336_W	2.750	1.00
167_I	189_V	2.694	1.00
337_K	341_I	2.657	1.00
95_N	122_Q	2.654	1.00
160_L	332_A	2.636	1.00
166_K	197_V	2.635	1.00
57_Q	61_E	2.579	1.00
69_E	290_E	2.576	1.00
203_A	234_T	2.563	1.00
162_S	165_G	2.540	1.00
187_A	347_P	2.538	1.00
166_K	199_E	2.456	1.00
51_D	57_Q	2.351	1.00
65_A	290_E	2.302	1.00
310_G	313_Q	2.232	1.00
332_A	335_K	2.219	1.00
326_S	329_V	2.157	1.00
174_E	179_K	2.139	1.00
162_S	225_D	2.127	1.00
98_A	128_F	2.101	1.00
334_D	337_K	2.099	1.00
161_S	326_S	2.081	1.00
64_Q	67_G	2.052	1.00
170_V	238_V	1.997	1.00
334_D	338_K	1.990	1.00
330_I	334_D	1.974	1.00
154_V	318_I	1.971	1.00
150_G	281_M	1.969	1.00
166_K	224_V	1.937	1.00
95_N	123_R	1.934	1.00
156_V	192_V	1.930	1.00
98_A	123_R	1.923	1.00
291_D	295_K	1.889	1.00
339_K	342_D	1.846	1.00
205_G	208_K	1.812	1.00
145_F	288_V	1.801	1.00
65_A	69_E	1.778	1.00
331_K	335_K	1.761	1.00
157_A	333_V	1.760	1.00
60_W	64_Q	1.752	1.00
317_G	337_K	1.742	1.00
327_D	330_I	1.737	1.00
156_V	191_A	1.728	1.00
191_A	336_W	1.721	1.00
64_Q	68_K	1.712	1.00
338_K	341_I	1.698	1.00
147_E	180_K	1.693	1.00
66_F	293_T	1.686	1.00
42_A	97_L	1.686	1.00
212_G	234_T	1.681	1.00
105_I	126_T	1.679	1.00
146_K	310_G	1.677	1.00
154_V	257_I	1.639	1.00
186_R	190_Q	1.639	1.00
339_K	344_L	1.588	1.00
182_E	186_R	1.573	1.00
221_K	252_R	1.562	1.00
311_L	341_I	1.548	1.00
161_S	329_V	1.536	1.00
213_K	244_N	1.532	1.00
159_A	162_S	1.524	1.00
295_K	300_K	1.506	1.00
209_A	234_T	1.472	1.00
159_A	196_A	1.448	1.00
213_K	217_E	1.435	1.00
42_A	102_F	1.426	1.00
332_A	336_W	1.416	1.00
220_Y	242_A	1.415	1.00
199_E	224_V	1.407	1.00
216_A	227_I	1.406	1.00
66_F	70_N	1.402	1.00
70_N	290_E	1.375	1.00
258_G	263_Q	1.370	1.00
39_F	296_A	1.362	1.00
331_K	334_D	1.348	1.00
314_D	317_G	1.343	1.00
94_L	107_G	1.340	1.00
70_N	294_K	1.334	1.00
213_K	240_T	1.309	1.00
164_S	225_D	1.301	1.00
149_E	312_D	1.288	1.00
146_K	149_E	1.285	1.00
135_V	142_S	1.285	1.00
59_A	289_V	1.255	1.00
153_L	337_K	1.247	1.00
162_S	226_V	1.239	1.00
336_W	339_K	1.238	1.00
217_E	221_K	1.229	1.00
203_A	215_T	1.226	1.00
113_E	133_A	1.226	1.00
69_E	294_K	1.215	1.00
284_K	287_T	1.200	1.00
95_N	99_R	1.196	1.00
152_F	336_W	1.191	1.00
16_A	19_I	1.188	1.00
65_A	286_D	1.182	1.00
153_L	333_V	1.177	1.00
154_V	281_M	1.176	1.00
89_D	92_T	1.169	1.00
40_S	102_F	1.164	1.00
19_I	22_A	1.153	1.00
95_N	119_I	1.142	1.00
82_L	97_L	1.139	1.00
15_A	18_T	1.135	1.00
104_L	293_T	1.130	1.00
50_V	81_Y	1.126	1.00
117_S	121_D	1.124	1.00
99_R	123_R	1.123	1.00
159_A	192_V	1.122	1.00
171_G	178_I	1.119	1.00
160_L	329_V	1.118	1.00
290_E	294_K	1.118	1.00
61_E	64_Q	1.114	1.00
157_A	279_T	1.113	1.00
48_G	56_N	1.110	1.00
168_G	224_V	1.109	1.00
101_N	126_T	1.109	1.00
150_G	316_V	1.109	1.00
166_K	223_G	1.090	0.99
20_L	23_C	1.086	0.99
253_D	276_V	1.074	0.99
141_A	301_F	1.074	0.99
91_T	95_N	1.073	0.99
214_A	217_E	1.065	0.99
255_W	324_N	1.064	0.99
162_S	189_V	1.062	0.99
130_I	133_A	1.060	0.99
239_F	277_T	1.056	0.99
243_K	275_N	1.055	0.99
41_V	104_L	1.050	0.99
39_F	297_S	1.048	0.99
108_V	131_I	1.041	0.99
291_D	294_K	1.037	0.99
97_L	102_F	1.036	0.99
279_T	319_S	1.029	0.99
94_L	119_I	1.027	0.99
50_V	60_W	1.027	0.99
279_T	320_P	1.019	0.99
284_K	317_G	1.013	0.99
255_W	278_L	1.005	0.99
220_Y	227_I	0.998	0.99
168_G	219_M	0.986	0.99
217_E	241_E	0.986	0.99
241_E	244_N	0.984	0.99
226_V	257_I	0.976	0.99
256_V	276_V	0.973	0.99
91_T	119_I	0.972	0.99
95_N	98_A	0.962	0.99
94_L	116_I	0.961	0.99
238_V	256_V	0.961	0.99
199_E	222_S	0.951	0.98
201_K	219_M	0.950	0.98
208_K	211_V	0.949	0.98
67_G	73_K	0.949	0.98
203_A	212_G	0.948	0.98
62_G	286_D	0.945	0.98
239_F	256_V	0.945	0.98
239_F	273_D	0.943	0.98
141_A	307_L	0.943	0.98
282_V	317_G	0.938	0.98
156_V	332_A	0.934	0.98
287_T	291_D	0.934	0.98
127_N	301_F	0.929	0.98
193_N	196_A	0.928	0.98
211_V	214_A	0.927	0.98
242_A	256_V	0.924	0.98
146_K	308_T	0.916	0.98
12_L	15_A	0.912	0.98
239_F	276_V	0.909	0.98
104_L	296_A	0.908	0.98
117_S	140_V	0.906	0.98
23_C	26_S	0.905	0.98
14_I	17_G	0.903	0.98
82_L	85_K	0.902	0.98
157_A	325_L	0.900	0.98
186_R	198_V	0.899	0.98
151_S	181_F	0.894	0.98
113_E	117_S	0.891	0.98
106_Y	129_A	0.882	0.97
84_S	93_N	0.881	0.97
228_Y	281_M	0.879	0.97
103_D	126_T	0.873	0.97
12_L	16_A	0.870	0.97
336_W	344_L	0.869	0.97
129_A	143_I	0.868	0.97
41_V	293_T	0.863	0.97
104_L	129_A	0.862	0.97
22_A	25_N	0.861	0.97
335_K	338_K	0.860	0.97
136_D	142_S	0.859	0.97
146_K	313_Q	0.848	0.97
264_Y	319_S	0.845	0.97
98_A	126_T	0.843	0.97
81_Y	84_S	0.841	0.97
86_S	89_D	0.838	0.97
309_Y	313_Q	0.835	0.97
151_S	281_M	0.834	0.96
201_K	215_T	0.832	0.96
219_M	224_V	0.831	0.96
256_V	277_T	0.829	0.96
328_D	331_K	0.827	0.96
48_G	52_D	0.826	0.96
80_D	102_F	0.824	0.96
258_G	280_S	0.823	0.96
156_V	160_L	0.819	0.96
50_V	57_Q	0.816	0.96
16_A	20_L	0.812	0.96
98_A	119_I	0.812	0.96
43_M	59_A	0.811	0.96
45_T	50_V	0.810	0.96
159_A	193_N	0.804	0.96
117_S	137_K	0.804	0.96
63_I	67_G	0.802	0.96
273_D	276_V	0.800	0.96
158_A	257_I	0.795	0.95
295_K	298_D	0.790	0.95
294_K	297_S	0.789	0.95
18_T	22_A	0.787	0.95
161_S	278_L	0.785	0.95
181_F	230_S	0.785	0.95
114_D	117_S	0.783	0.95
186_R	200_V	0.783	0.95
152_F	188_G	0.781	0.95
161_S	226_V	0.778	0.95
116_I	140_V	0.776	0.95
157_A	161_S	0.774	0.95
94_L	112_M	0.772	0.95
264_Y	274_D	0.771	0.95
39_F	103_D	0.768	0.94
43_M	63_I	0.765	0.94
240_T	243_K	0.765	0.94
169_F	230_S	0.761	0.94
260_D	283_K	0.760	0.94
165_G	225_D	0.759	0.94
65_A	287_T	0.756	0.94
127_N	296_A	0.753	0.94
45_T	60_W	0.753	0.94
149_E	310_G	0.750	0.94
162_S	196_A	0.747	0.94
217_E	252_R	0.747	0.94
157_A	257_I	0.745	0.94
188_G	191_A	0.744	0.93
172_G	232_G	0.743	0.93
314_D	341_I	0.740	0.93
19_I	23_C	0.739	0.93
145_F	283_K	0.736	0.93
45_T	81_Y	0.735	0.93
164_S	223_G	0.733	0.93
66_F	72_L	0.732	0.93
59_A	285_V	0.732	0.93
66_F	290_E	0.730	0.93
132_D	283_K	0.729	0.93
62_G	66_F	0.727	0.93
131_I	285_V	0.725	0.93
160_L	192_V	0.721	0.92
189_V	198_V	0.720	0.92
58_S	286_D	0.720	0.92
63_I	79_Y	0.717	0.92
328_D	332_A	0.717	0.92
13_V	19_I	0.716	0.92
230_S	259_V	0.714	0.92
65_A	68_K	0.713	0.92
94_L	98_A	0.711	0.92
340_I	344_L	0.710	0.92
113_E	135_V	0.705	0.92
169_F	182_E	0.702	0.91
181_F	259_V	0.701	0.91
156_V	189_V	0.701	0.91
141_A	292_V	0.699	0.91
37_N	103_D	0.698	0.91
53_K	266_E	0.695	0.91
259_V	283_K	0.693	0.91
47_V	207_D	0.693	0.91
152_F	156_V	0.692	0.91
216_A	220_Y	0.691	0.91
257_I	279_T	0.691	0.91
70_N	297_S	0.688	0.91
181_F	260_D	0.688	0.91
125_N	138_D	0.688	0.91
96_K	100_E	0.688	0.91
99_R	122_Q	0.688	0.91
102_F	126_T	0.687	0.90
231_A	235_G	0.685	0.90
311_L	340_I	0.685	0.90
211_V	215_T	0.685	0.90
106_Y	293_T	0.684	0.90
187_A	190_Q	0.684	0.90
118_E	122_Q	0.683	0.90
177_L	180_K	0.683	0.90
12_L	18_T	0.683	0.90
182_E	200_V	0.681	0.90
182_E	202_Y	0.678	0.90
59_A	108_V	0.678	0.90
91_T	115_S	0.677	0.90
156_V	333_V	0.676	0.90
148_Q	346_I	0.676	0.90
40_S	103_D	0.674	0.90
10_M	13_V	0.674	0.90
210_D	213_K	0.673	0.90
147_E	281_M	0.672	0.89
199_E	219_M	0.671	0.89
262_D	320_P	0.670	0.89
18_T	21_G	0.670	0.89
243_K	276_V	0.669	0.89
174_E	204_G	0.668	0.89
229_H	238_V	0.668	0.89
11_S	15_A	0.667	0.89
153_L	311_L	0.666	0.89
11_S	14_I	0.666	0.89
137_K	140_V	0.665	0.89
142_S	305_E	0.665	0.89
282_V	319_S	0.663	0.89
143_I	292_V	0.663	0.89
166_K	222_S	0.662	0.89
214_A	218_S	0.662	0.89
61_E	261_K	0.662	0.89
147_E	283_K	0.662	0.89
264_Y	321_S	0.659	0.89
226_V	278_L	0.657	0.88
11_S	18_T	0.657	0.88
151_S	230_S	0.657	0.88
159_A	165_G	0.655	0.88
170_V	216_A	0.652	0.88
242_A	253_D	0.652	0.88
141_A	302_P	0.650	0.88
217_E	244_N	0.648	0.88
23_C	27_E	0.646	0.88
163_K	195_K	0.646	0.88
325_L	329_V	0.644	0.87
231_A	237_G	0.643	0.87
277_T	280_S	0.642	0.87
190_Q	194_P	0.641	0.87
191_A	194_P	0.640	0.87
215_T	219_M	0.640	0.87
113_E	130_I	0.639	0.87
148_Q	180_K	0.639	0.87
314_D	337_K	0.639	0.87
329_V	333_V	0.634	0.87
60_W	63_I	0.633	0.87
235_G	259_V	0.632	0.86
161_S	255_W	0.628	0.86
292_V	295_K	0.628	0.86
119_I	122_Q	0.626	0.86
13_V	17_G	0.626	0.86
87_D	111_L	0.623	0.86
311_L	344_L	0.621	0.85
52_D	57_Q	0.619	0.85
134_V	142_S	0.618	0.85
220_Y	241_E	0.618	0.85
151_S	228_Y	0.617	0.85
165_G	195_K	0.616	0.85
52_D	56_N	0.615	0.85
129_A	292_V	0.614	0.85
275_N	323_Q	0.613	0.85
121_D	137_K	0.613	0.85
212_G	235_G	0.612	0.85
10_M	17_G	0.611	0.85
134_V	144_T	0.607	0.84
167_I	185_F	0.607	0.84
143_I	309_Y	0.602	0.84
60_W	81_Y	0.602	0.84
173_M	206_F	0.600	0.83
17_G	20_L	0.600	0.83
244_N	252_R	0.600	0.83
81_Y	85_K	0.599	0.83
310_G	315_G	0.599	0.83
40_S	73_K	0.598	0.83
103_D	125_N	0.597	0.83
212_G	237_G	0.595	0.83
191_A	339_K	0.592	0.83
67_G	72_L	0.591	0.83
168_G	216_A	0.589	0.82
302_P	305_E	0.588	0.82
151_S	177_L	0.587	0.82
171_G	182_E	0.586	0.82
192_V	196_A	0.585	0.82
154_V	279_T	0.585	0.82
84_S	89_D	0.584	0.82
325_L	330_I	0.584	0.82
47_V	111_L	0.584	0.82
291_D	313_Q	0.581	0.82
296_A	301_F	0.581	0.82
130_I	135_V	0.580	0.81
287_T	290_E	0.580	0.81
108_V	143_I	0.579	0.81
233_A	236_T	0.579	0.81
15_A	19_I	0.578	0.81
295_K	302_P	0.576	0.81
157_A	329_V	0.576	0.81
68_K	125_N	0.575	0.81
311_L	337_K	0.575	0.81
89_D	93_N	0.574	0.81
82_L	93_N	0.571	0.80
279_T	321_S	0.568	0.80
119_I	128_F	0.567	0.80
68_K	300_K	0.562	0.79
144_T	180_K	0.561	0.79
10_M	16_A	0.560	0.79
50_V	64_Q	0.558	0.79
20_L	27_E	0.556	0.79
157_A	160_L	0.555	0.79
20_L	25_N	0.553	0.78
213_K	241_E	0.552	0.78
170_V	229_H	0.552	0.78
318_I	333_V	0.551	0.78
262_D	280_S	0.549	0.78
187_A	191_A	0.548	0.78
66_F	297_S	0.548	0.78
295_K	307_L	0.546	0.77
167_I	196_A	0.546	0.77
266_E	274_D	0.546	0.77
55_F	181_F	0.545	0.77
55_F	132_D	0.545	0.77
14_I	18_T	0.545	0.77
275_N	324_N	0.542	0.77
171_G	202_Y	0.542	0.77
131_I	289_V	0.542	0.77
144_T	308_T	0.538	0.76
179_K	202_Y	0.537	0.76
13_V	16_A	0.537	0.76
189_V	192_V	0.535	0.76
185_F	188_G	0.535	0.76
10_M	14_I	0.533	0.76
177_L	228_Y	0.530	0.75
61_E	286_D	0.529	0.75
160_L	328_D	0.529	0.75
262_D	319_S	0.526	0.75
169_F	178_I	0.526	0.75
18_T	23_C	0.526	0.75
127_N	141_A	0.525	0.75
12_L	22_A	0.523	0.74
90_Y	112_M	0.523	0.74
58_S	261_K	0.523	0.74
183_V	349_T	0.521	0.74
185_F	228_Y	0.521	0.74
97_L	105_I	0.520	0.74
263_Q	266_E	0.520	0.74
207_D	234_T	0.519	0.74
162_S	167_I	0.519	0.74
184_G	283_K	0.518	0.74
291_D	309_Y	0.518	0.74
240_T	244_N	0.518	0.74
60_W	67_G	0.517	0.74
38_K	73_K	0.516	0.73
67_G	70_N	0.514	0.73
173_M	204_G	0.514	0.73
161_S	325_L	0.513	0.73
318_I	337_K	0.512	0.73
105_I	128_F	0.511	0.73
54_S	263_Q	0.511	0.73
66_F	79_Y	0.511	0.73
141_A	295_K	0.510	0.73
132_D	232_G	0.510	0.73
181_F	283_K	0.509	0.72
231_A	234_T	0.508	0.72
44_V	93_N	0.507	0.72
190_Q	197_V	0.507	0.72
154_V	157_A	0.505	0.72
229_H	235_G	0.501	0.71
153_L	318_I	0.501	0.71
53_K	57_Q	0.501	0.71
62_G	287_T	0.500	0.71

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4pevA	1	0.8579	100	0.418	Contact Map
4iilA	1	0.8134	100	0.449	Contact Map
3s99A	1	0.8329	100	0.45	Contact Map
4p98A	1	0.805	100	0.46	Contact Map
2fqxA	1	0.8663	100	0.472	Contact Map
2hqbA	1	0.7827	100	0.487	Contact Map
4ycsA	1	0.337	100	0.646	Contact Map
4wzzA	1	0.7967	99.9	0.713	Contact Map
4yv7A	1	0.7772	99.8	0.753	Contact Map
4z0nA	1	0.7716	99.8	0.759	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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