GREMLIN Database
MRPF - Na(+)/H(+) antiporter subunit F
UniProt: O05228 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 94 (88)
Sequences: 828 (543)
Seq/√Len: 57.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
8_I12_I4.7721.00
17_T40_I3.3961.00
4_L8_I2.6331.00
13_M50_L2.5681.00
22_I26_K2.1801.00
8_I11_G1.9521.00
16_S84_K1.9401.00
24_V36_A1.8971.00
50_L53_I1.8941.00
15_V19_L1.8801.00
52_S61_L1.8701.00
41_G67_L1.8160.99
3_T26_K1.7940.99
5_I9_A1.7900.99
80_K86_E1.7530.99
84_K89_E1.6590.99
20_Y40_I1.6190.99
67_L70_L1.6130.99
51_V55_L1.5120.98
20_Y66_L1.4560.97
36_A40_I1.4470.97
9_A50_L1.4440.97
65_L70_L1.4250.97
20_Y43_N1.3240.95
4_L7_Q1.3230.95
41_G70_L1.3020.95
38_D77_A1.2830.95
18_F21_V1.2750.94
52_S71_S1.2610.94
37_L74_G1.2380.93
67_L88_I1.2050.93
3_T7_Q1.1980.92
10_L54_L1.1790.92
41_G44_L1.1650.91
39_A43_N1.1410.90
65_L80_K1.1400.90
10_L47_I1.1230.90
49_A68_G1.0940.88
2_F59_A1.0820.88
46_A86_E1.0760.87
2_F6_L1.0620.87
43_N46_A1.0520.86
40_I69_I1.0410.86
24_V27_G1.0160.84
34_V78_F1.0130.84
62_D80_K1.0130.84
37_L79_S1.0110.84
38_D75_T1.0080.84
29_T66_L1.0050.84
65_L85_G0.9980.83
46_A51_V0.9790.82
2_F5_I0.9750.82
4_L9_A0.9540.80
26_K40_I0.9530.80
45_I80_K0.9330.79
60_F83_E0.9290.79
18_F68_G0.9150.77
23_R28_P0.9000.76
50_L87_I0.8870.75
19_L69_I0.8750.74
43_N83_E0.8660.73
23_R27_G0.8610.73
47_I54_L0.8600.73
72_F75_T0.8560.73
45_I71_S0.8350.71
7_Q14_A0.8310.70
34_V82_L0.8290.70
46_A81_F0.8150.69
61_L89_E0.8050.68
79_S88_I0.8030.68
34_V61_L0.8010.68
38_D70_L0.7930.67
16_S31_P0.7500.62
3_T14_A0.7480.62
31_P81_F0.7440.62
45_I67_L0.7210.59
6_L50_L0.7120.58
19_L25_I0.7120.58
9_A54_L0.7050.58
21_V66_L0.7050.58
53_I77_A0.6770.55
35_V61_L0.6760.54
34_V37_L0.6750.54
74_G80_K0.6690.54
7_Q54_L0.6610.53
44_L54_L0.6550.52
54_L87_I0.6480.51
16_S19_L0.6470.51
19_L50_L0.6430.51
59_A71_S0.6420.51
39_A42_I0.6410.51
48_T63_I0.6360.50
13_M70_L0.6330.50
53_I56_K0.6270.49
47_I88_I0.6210.48
66_L80_K0.6170.48
20_Y39_A0.6160.48
71_S74_G0.6110.47
10_L25_I0.5880.45
50_L69_I0.5850.44
82_L86_E0.5840.44
29_T49_A0.5820.44
31_P80_K0.5810.44
30_V87_I0.5770.44
18_F56_K0.5760.43
70_L75_T0.5730.43
17_T24_V0.5650.42
12_I69_I0.5630.42
10_L29_T0.5590.42
15_V21_V0.5570.41
73_I88_I0.5560.41
42_I50_L0.5530.41
74_G82_L0.5510.41
13_M16_S0.5500.41
54_L58_S0.5390.40
14_A18_F0.5380.39
14_A87_I0.5340.39
5_I24_V0.5310.39
30_V56_K0.5240.38
49_A76_I0.5240.38
61_L64_I0.5200.38
24_V81_F0.5190.37
35_V52_S0.5180.37
70_L74_G0.5150.37
35_V75_T0.5140.37
19_L22_I0.5110.37
39_A69_I0.5100.37
11_G31_P0.5090.36
41_G83_E0.5060.36
34_V86_E0.5020.36
9_A43_N0.5000.35
7_Q15_V0.5000.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3j08A 2 0.9468 16.2 0.919 Contact Map
4bbjA 1 0.9362 15.7 0.92 Contact Map
3j09A 2 0.9468 13.8 0.922 Contact Map
1oqwA 1 0.3936 13.4 0.922 Contact Map
2hi2A 1 0.383 10 0.927 Contact Map
3sokA 1 0.3936 8.2 0.929 Contact Map
1n2sA 2 0.3511 6.9 0.932 Contact Map
3st7A 2 0.3511 6.6 0.933 Contact Map
3iusA 1 0.3511 5.9 0.934 Contact Map
3eheA 2 0.3404 5.7 0.934 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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